Identification and analysis of the tissue factor pathway inhibitor 2 of Sciaenops ocellatus
Tissue factor pathway inhibitor 2 (TFPI-2) is a structural homologue of TFPI, a potent inhibitor of tissue factor (TF)-mediated coagulation. Although TFPI-2 has been identified at sequence level in several fish species, no study on piscine TFPI-2 has been documented. In this report, we identified an...
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ftchinacasciocas:oai:ir.qdio.ac.cn:337002/11869 2023-05-15T18:06:06+02:00 Identification and analysis of the tissue factor pathway inhibitor 2 of Sciaenops ocellatus Zhang, Min Xiao, Zhi-zhong Sun, Li 2011 http://ir.qdio.ac.cn/handle/337002/11870 https://doi.org/10.1016/j.fsi.2010.10.003 英语 eng FISH & SHELLFISH IMMUNOLOGY Zhang, Min; Xiao, Zhizhong; Sun, Li.Identification and analysis of the tissue factor pathway inhibitor 2 of Sciaenops ocellatus,FISH & SHELLFISH IMMUNOLOGY,2011,30(1):209-214 http://ir.qdio.ac.cn/handle/337002/11870 doi:10.1016/j.fsi.2010.10.003 Tissue Factor Pathway Inhibitor 2 Sciaenops Ocellatus Bactericidal Activity Fisheries Immunology Marine & Freshwater Biology Veterinary Sciences Article 期刊论文 2011 ftchinacasciocas https://doi.org/10.1016/j.fsi.2010.10.003 2022-06-27T05:34:13Z Tissue factor pathway inhibitor 2 (TFPI-2) is a structural homologue of TFPI, a potent inhibitor of tissue factor (TF)-mediated coagulation. Although TFPI-2 has been identified at sequence level in several fish species, no study on piscine TFPI-2 has been documented. In this report, we identified and analyzed a TFPI-2 homologue, SoTFPI2, from red drum Sciaenops ocellatus. The open reading frame of SoTFPI2 is 681 bp, which encodes a 226-residue protein that shares 59.2-82.3% overall sequence identities with known fish TFPI-2. SoTFPI2 possesses three tandem Kunitz domains and is negatively charged at the N-terminus and positively charged at the C-terminus. Expression of SoTFPI2 was detected, in increasing order, in spleen, muscle, gill, brain, liver, kidney, blood, and heart. Bacterial challenge and lipopolysaccharide treatment significantly upregulated SoTFPI2 expression in kidney in time-dependent manners. Recombinant SoTFPI2 purified from Escherichia colt inhibits the proteolytic activity of trypsin and exhibits bactericidal effect on a fish pathogen. Take together, these results indicate that SoTFPI2 is a biologically active serine protease inhibitor with antibacterial property and is likely to play a role in anti-bacterial infection. (C) 2010 Elsevier Ltd. All rights reserved. Tissue factor pathway inhibitor 2 (TFPI-2) is a structural homologue of TFPI, a potent inhibitor of tissue factor (TF)-mediated coagulation. Although TFPI-2 has been identified at sequence level in several fish species, no study on piscine TFPI-2 has been documented. In this report, we identified and analyzed a TFPI-2 homologue, SoTFPI2, from red drum Sciaenops ocellatus. The open reading frame of SoTFPI2 is 681 bp, which encodes a 226-residue protein that shares 59.2-82.3% overall sequence identities with known fish TFPI-2. SoTFPI2 possesses three tandem Kunitz domains and is negatively charged at the N-terminus and positively charged at the C-terminus. Expression of SoTFPI2 was detected, in increasing order, in spleen, muscle, gill, ... Article in Journal/Newspaper Red drum Sciaenops ocellatus Institute of Oceanology, Chinese Academy of Sciences: IOCAS-IR Fish & Shellfish Immunology 30 1 209 214 |
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Open Polar |
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Institute of Oceanology, Chinese Academy of Sciences: IOCAS-IR |
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ftchinacasciocas |
language |
English |
topic |
Tissue Factor Pathway Inhibitor 2 Sciaenops Ocellatus Bactericidal Activity Fisheries Immunology Marine & Freshwater Biology Veterinary Sciences |
spellingShingle |
Tissue Factor Pathway Inhibitor 2 Sciaenops Ocellatus Bactericidal Activity Fisheries Immunology Marine & Freshwater Biology Veterinary Sciences Zhang, Min Xiao, Zhi-zhong Sun, Li Identification and analysis of the tissue factor pathway inhibitor 2 of Sciaenops ocellatus |
topic_facet |
Tissue Factor Pathway Inhibitor 2 Sciaenops Ocellatus Bactericidal Activity Fisheries Immunology Marine & Freshwater Biology Veterinary Sciences |
description |
Tissue factor pathway inhibitor 2 (TFPI-2) is a structural homologue of TFPI, a potent inhibitor of tissue factor (TF)-mediated coagulation. Although TFPI-2 has been identified at sequence level in several fish species, no study on piscine TFPI-2 has been documented. In this report, we identified and analyzed a TFPI-2 homologue, SoTFPI2, from red drum Sciaenops ocellatus. The open reading frame of SoTFPI2 is 681 bp, which encodes a 226-residue protein that shares 59.2-82.3% overall sequence identities with known fish TFPI-2. SoTFPI2 possesses three tandem Kunitz domains and is negatively charged at the N-terminus and positively charged at the C-terminus. Expression of SoTFPI2 was detected, in increasing order, in spleen, muscle, gill, brain, liver, kidney, blood, and heart. Bacterial challenge and lipopolysaccharide treatment significantly upregulated SoTFPI2 expression in kidney in time-dependent manners. Recombinant SoTFPI2 purified from Escherichia colt inhibits the proteolytic activity of trypsin and exhibits bactericidal effect on a fish pathogen. Take together, these results indicate that SoTFPI2 is a biologically active serine protease inhibitor with antibacterial property and is likely to play a role in anti-bacterial infection. (C) 2010 Elsevier Ltd. All rights reserved. Tissue factor pathway inhibitor 2 (TFPI-2) is a structural homologue of TFPI, a potent inhibitor of tissue factor (TF)-mediated coagulation. Although TFPI-2 has been identified at sequence level in several fish species, no study on piscine TFPI-2 has been documented. In this report, we identified and analyzed a TFPI-2 homologue, SoTFPI2, from red drum Sciaenops ocellatus. The open reading frame of SoTFPI2 is 681 bp, which encodes a 226-residue protein that shares 59.2-82.3% overall sequence identities with known fish TFPI-2. SoTFPI2 possesses three tandem Kunitz domains and is negatively charged at the N-terminus and positively charged at the C-terminus. Expression of SoTFPI2 was detected, in increasing order, in spleen, muscle, gill, ... |
format |
Article in Journal/Newspaper |
author |
Zhang, Min Xiao, Zhi-zhong Sun, Li |
author_facet |
Zhang, Min Xiao, Zhi-zhong Sun, Li |
author_sort |
Zhang, Min |
title |
Identification and analysis of the tissue factor pathway inhibitor 2 of Sciaenops ocellatus |
title_short |
Identification and analysis of the tissue factor pathway inhibitor 2 of Sciaenops ocellatus |
title_full |
Identification and analysis of the tissue factor pathway inhibitor 2 of Sciaenops ocellatus |
title_fullStr |
Identification and analysis of the tissue factor pathway inhibitor 2 of Sciaenops ocellatus |
title_full_unstemmed |
Identification and analysis of the tissue factor pathway inhibitor 2 of Sciaenops ocellatus |
title_sort |
identification and analysis of the tissue factor pathway inhibitor 2 of sciaenops ocellatus |
publishDate |
2011 |
url |
http://ir.qdio.ac.cn/handle/337002/11870 https://doi.org/10.1016/j.fsi.2010.10.003 |
genre |
Red drum Sciaenops ocellatus |
genre_facet |
Red drum Sciaenops ocellatus |
op_relation |
FISH & SHELLFISH IMMUNOLOGY Zhang, Min; Xiao, Zhizhong; Sun, Li.Identification and analysis of the tissue factor pathway inhibitor 2 of Sciaenops ocellatus,FISH & SHELLFISH IMMUNOLOGY,2011,30(1):209-214 http://ir.qdio.ac.cn/handle/337002/11870 doi:10.1016/j.fsi.2010.10.003 |
op_doi |
https://doi.org/10.1016/j.fsi.2010.10.003 |
container_title |
Fish & Shellfish Immunology |
container_volume |
30 |
container_issue |
1 |
container_start_page |
209 |
op_container_end_page |
214 |
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1766177683360710656 |