The immunological capacity in the larvae of Pacific oyster Crassostrea gigas
As the immune system has not fully developed during early developmental stages, bivalve larvae are more susceptible for pathogens, which frequently leads to the significant mortality in hatcheries. In the present study, the development of immune system and its response against bacteria challenge wer...
Published in: | Fish & Shellfish Immunology |
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ftchinacasciocas:oai:ir.qdio.ac.cn:337002/112487 2023-05-15T15:57:55+02:00 The immunological capacity in the larvae of Pacific oyster Crassostrea gigas Song, Xiaorui Wang, Hao Xin, Lusheng Xu, Jiachao Jia, Zhihao Wang, Lingling Song, Linsheng 2016-02-01 http://ir.qdio.ac.cn/handle/337002/112487 https://doi.org/10.1016/j.fsi.2016.01.009 英语 eng FISH & SHELLFISH IMMUNOLOGY Song, Xiaorui,Wang, Hao,Xin, Lusheng,et al. The immunological capacity in the larvae of Pacific oyster Crassostrea gigas[J]. FISH & SHELLFISH IMMUNOLOGY,2016,49:461-469. http://ir.qdio.ac.cn/handle/337002/112487 doi:10.1016/j.fsi.2016.01.009 Pacific Oyster Crassostrea Gigas Phagocytosis Immune System Hatching Trochophore Stage Umbo Larvae Article 期刊论文 2016 ftchinacasciocas https://doi.org/10.1016/j.fsi.2016.01.009 2022-06-27T05:37:29Z As the immune system has not fully developed during early developmental stages, bivalve larvae are more susceptible for pathogens, which frequently leads to the significant mortality in hatcheries. In the present study, the development of immune system and its response against bacteria challenge were investigated in order to characterize the repertoire of immunological capacity of Pacific oyster Crassostrea gigas during the ontogenesis. The phagocytosis was firstly observed in the early D-veliger larvae (17 hpf), especially in their velum site, which indicated the appearance of functional hemocytes during early Dveliger larvae stage. The whole-mount immunofluorescence assay of three pattern recognition receptors (integrin beta-1, caspase-3 and C-type lectin 3) and one immune effector gene (IL17-5) was performed in blastula, early D-veliger and umbo larvae, suggested that velum and digestive gland were the potential sites of immune system in the larvae. The lowest activities of antioxidant enzymes (superoxide dismutase and catalase) and hydrolytic enzyme (lysozyme), as well as descended expression levels of 12 immune genes at the transition between embryogenesis and planktonic, indicated that the larvae at hatching (9 hpf) were in hypo-immunity. While the ascending activities of enzymes and expression levels of seven immune genes during the trochophore stage (15 hpf) suggested the initiation of immune system. The steadily increasing trend of all the 12 candidate genes at the early umbo larvae (120 h) hinted that the immune system was well developed at this stage. After bacterial challenge, some immune recognition (TLR4) and immune effector (1L17-5 and defh2) genes were activated in blastula stage (4 hpf), and other immune genes were up regulated in D-veliger larvae, indicating that the zygotic immune system could respond earlier against the bacterial challenge during its development. These results indicated that the cellular and humoral immune components appeared at trochophore stage, and the cellular immune ... Article in Journal/Newspaper Crassostrea gigas Pacific oyster Institute of Oceanology, Chinese Academy of Sciences: IOCAS-IR Pacific Fish & Shellfish Immunology 49 461 469 |
institution |
Open Polar |
collection |
Institute of Oceanology, Chinese Academy of Sciences: IOCAS-IR |
op_collection_id |
ftchinacasciocas |
language |
English |
topic |
Pacific Oyster Crassostrea Gigas Phagocytosis Immune System Hatching Trochophore Stage Umbo Larvae |
spellingShingle |
Pacific Oyster Crassostrea Gigas Phagocytosis Immune System Hatching Trochophore Stage Umbo Larvae Song, Xiaorui Wang, Hao Xin, Lusheng Xu, Jiachao Jia, Zhihao Wang, Lingling Song, Linsheng The immunological capacity in the larvae of Pacific oyster Crassostrea gigas |
topic_facet |
Pacific Oyster Crassostrea Gigas Phagocytosis Immune System Hatching Trochophore Stage Umbo Larvae |
description |
As the immune system has not fully developed during early developmental stages, bivalve larvae are more susceptible for pathogens, which frequently leads to the significant mortality in hatcheries. In the present study, the development of immune system and its response against bacteria challenge were investigated in order to characterize the repertoire of immunological capacity of Pacific oyster Crassostrea gigas during the ontogenesis. The phagocytosis was firstly observed in the early D-veliger larvae (17 hpf), especially in their velum site, which indicated the appearance of functional hemocytes during early Dveliger larvae stage. The whole-mount immunofluorescence assay of three pattern recognition receptors (integrin beta-1, caspase-3 and C-type lectin 3) and one immune effector gene (IL17-5) was performed in blastula, early D-veliger and umbo larvae, suggested that velum and digestive gland were the potential sites of immune system in the larvae. The lowest activities of antioxidant enzymes (superoxide dismutase and catalase) and hydrolytic enzyme (lysozyme), as well as descended expression levels of 12 immune genes at the transition between embryogenesis and planktonic, indicated that the larvae at hatching (9 hpf) were in hypo-immunity. While the ascending activities of enzymes and expression levels of seven immune genes during the trochophore stage (15 hpf) suggested the initiation of immune system. The steadily increasing trend of all the 12 candidate genes at the early umbo larvae (120 h) hinted that the immune system was well developed at this stage. After bacterial challenge, some immune recognition (TLR4) and immune effector (1L17-5 and defh2) genes were activated in blastula stage (4 hpf), and other immune genes were up regulated in D-veliger larvae, indicating that the zygotic immune system could respond earlier against the bacterial challenge during its development. These results indicated that the cellular and humoral immune components appeared at trochophore stage, and the cellular immune ... |
format |
Article in Journal/Newspaper |
author |
Song, Xiaorui Wang, Hao Xin, Lusheng Xu, Jiachao Jia, Zhihao Wang, Lingling Song, Linsheng |
author_facet |
Song, Xiaorui Wang, Hao Xin, Lusheng Xu, Jiachao Jia, Zhihao Wang, Lingling Song, Linsheng |
author_sort |
Song, Xiaorui |
title |
The immunological capacity in the larvae of Pacific oyster Crassostrea gigas |
title_short |
The immunological capacity in the larvae of Pacific oyster Crassostrea gigas |
title_full |
The immunological capacity in the larvae of Pacific oyster Crassostrea gigas |
title_fullStr |
The immunological capacity in the larvae of Pacific oyster Crassostrea gigas |
title_full_unstemmed |
The immunological capacity in the larvae of Pacific oyster Crassostrea gigas |
title_sort |
immunological capacity in the larvae of pacific oyster crassostrea gigas |
publishDate |
2016 |
url |
http://ir.qdio.ac.cn/handle/337002/112487 https://doi.org/10.1016/j.fsi.2016.01.009 |
geographic |
Pacific |
geographic_facet |
Pacific |
genre |
Crassostrea gigas Pacific oyster |
genre_facet |
Crassostrea gigas Pacific oyster |
op_relation |
FISH & SHELLFISH IMMUNOLOGY Song, Xiaorui,Wang, Hao,Xin, Lusheng,et al. The immunological capacity in the larvae of Pacific oyster Crassostrea gigas[J]. FISH & SHELLFISH IMMUNOLOGY,2016,49:461-469. http://ir.qdio.ac.cn/handle/337002/112487 doi:10.1016/j.fsi.2016.01.009 |
op_doi |
https://doi.org/10.1016/j.fsi.2016.01.009 |
container_title |
Fish & Shellfish Immunology |
container_volume |
49 |
container_start_page |
461 |
op_container_end_page |
469 |
_version_ |
1766393617941790720 |