Development and characterization of microsatellite markers for the Pacific Oyster Crassostrea gigas
Thirty-three polymorphic microsatellite DNA markers were developed and characterized for the Pacific Oyster Crassostrea gigas, a widespread oyster species with great economic and ecological importance, by the end-sequencing of an in-house fosmid library. The polymorphism of each locus was analyzed b...
Published in: | Conservation Genetics Resources |
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Main Authors: | , , , |
Format: | Article in Journal/Newspaper |
Language: | English |
Published: |
2009
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Subjects: | |
Online Access: | http://ir.qdio.ac.cn/handle/337002/10581 https://doi.org/10.1007/s12686-009-9104-x |
Summary: | Thirty-three polymorphic microsatellite DNA markers were developed and characterized for the Pacific Oyster Crassostrea gigas, a widespread oyster species with great economic and ecological importance, by the end-sequencing of an in-house fosmid library. The polymorphism of each locus was analyzed by screening 30 Pacific oysters from a natural population. The size of repeat motif ranged from 2 to 6 and the number of alleles per locus ranged from 3 to 9. The observed and expected heterozygosity ranged from 0.080 to 0.880 and from 0.174 to 0.876, respectively. These microsatellite markers will contribute to the increasing genetic studies in C. gigas. |
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