An efficient approach to eliminate steryl ethers and miscellaneous esters/ketones for gas chromatographic analysis of alkenones and alkenoates
Long-chain alkenones (LCAs) and alkenoates (LCEs) are highly valuable biomarkers for paleotemperature reconstructions. A major problem, however, for accurate quantification of these compounds using gas chromatography (GC) is co-elution with steryl ethers, wax esters, saturated ketones and other nume...
Published in: | Journal of Chromatography A |
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Language: | English |
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Online Access: | http://ir.ieecas.cn/handle/361006/13849 http://ir.ieecas.cn/handle/361006/13850 https://doi.org/10.1016/j.chroma.2019.02.064 |
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ftchinacascieeca:oai:ir.ieecas.cn:361006/13850 2023-06-11T04:14:13+02:00 An efficient approach to eliminate steryl ethers and miscellaneous esters/ketones for gas chromatographic analysis of alkenones and alkenoates Wang, Li Longo, William M. Dillon, James T. Zhao, Jiaju Zheng, Yinsui Moros, Matthias Huang, Yongsong 2019-07-05 http://ir.ieecas.cn/handle/361006/13849 http://ir.ieecas.cn/handle/361006/13850 https://doi.org/10.1016/j.chroma.2019.02.064 英语 eng ELSEVIER SCIENCE BV JOURNAL OF CHROMATOGRAPHY A http://ir.ieecas.cn/handle/361006/13849 http://ir.ieecas.cn/handle/361006/13850 doi:10.1016/j.chroma.2019.02.064 Alkenones Steryl ethers Clean-up Silver thiolate chromatographic material Paleotemperature reconstructions LONG-CHAIN ALKENONES POLYUNSATURATED FATTY-ACIDS NORDIC SEAS SEPARATION TEMPERATURE SEDIMENTS SURFACE PALEOTEMPERATURE IDENTIFICATION DISTRIBUTIONS Biochemistry & Molecular Biology Chemistry Biochemical Research Methods Analytical 期刊论文 2019 ftchinacascieeca https://doi.org/10.1016/j.chroma.2019.02.064 2023-05-08T13:24:31Z Long-chain alkenones (LCAs) and alkenoates (LCEs) are highly valuable biomarkers for paleotemperature reconstructions. A major problem, however, for accurate quantification of these compounds using gas chromatography (GC) is co-elution with steryl ethers, wax esters, saturated ketones and other numerous mid-polarity compounds frequently encountered in marginal marine and lake sediments. Co-elution during GC separation is prevalent, particularly if the full homologous series of alkenones and alkenoates are to be analyzed. Taking advantage of the presence of two or more double bonds in LCAs and LCEs, the conventional silica gel impregnated with silver nitrate has previously been used to remove co-eluting compounds for LCAs. However, this conventional argentation chromatography is hampered by the extreme instability of silver nitrate, poor reproducibility, low recovery and short lifetime. Here we demonstrate a highly efficient flash chromatographic approach based on silver thiolate chromatographic material (AgTCM) that overcomes the shortcomings of the traditional argentation chromatography and allows repeated sample preparation (up to 62 samples in one test) with little loss in separation efficiency. AgTCM selectively extracts LCAs and LCEs and effectively eliminates co-eluting compounds including steryl ethers and wax esters for the subsequent gas chromatography (GC) analysis. This new method, therefore, allows low-cost and high-throughput sample preparation for comprehensive quantification of the full homologous series of LCAs and LCEs in marine and lake sediments. (C) 2019 Elsevier B.V. All rights reserved. Report Nordic Seas Institute of Earth Environment: IEECAS OpenIR (Chinese Academy of Sciences) Journal of Chromatography A 1596 175 182 |
institution |
Open Polar |
collection |
Institute of Earth Environment: IEECAS OpenIR (Chinese Academy of Sciences) |
op_collection_id |
ftchinacascieeca |
language |
English |
topic |
Alkenones Steryl ethers Clean-up Silver thiolate chromatographic material Paleotemperature reconstructions LONG-CHAIN ALKENONES POLYUNSATURATED FATTY-ACIDS NORDIC SEAS SEPARATION TEMPERATURE SEDIMENTS SURFACE PALEOTEMPERATURE IDENTIFICATION DISTRIBUTIONS Biochemistry & Molecular Biology Chemistry Biochemical Research Methods Analytical |
spellingShingle |
Alkenones Steryl ethers Clean-up Silver thiolate chromatographic material Paleotemperature reconstructions LONG-CHAIN ALKENONES POLYUNSATURATED FATTY-ACIDS NORDIC SEAS SEPARATION TEMPERATURE SEDIMENTS SURFACE PALEOTEMPERATURE IDENTIFICATION DISTRIBUTIONS Biochemistry & Molecular Biology Chemistry Biochemical Research Methods Analytical Wang, Li Longo, William M. Dillon, James T. Zhao, Jiaju Zheng, Yinsui Moros, Matthias Huang, Yongsong An efficient approach to eliminate steryl ethers and miscellaneous esters/ketones for gas chromatographic analysis of alkenones and alkenoates |
topic_facet |
Alkenones Steryl ethers Clean-up Silver thiolate chromatographic material Paleotemperature reconstructions LONG-CHAIN ALKENONES POLYUNSATURATED FATTY-ACIDS NORDIC SEAS SEPARATION TEMPERATURE SEDIMENTS SURFACE PALEOTEMPERATURE IDENTIFICATION DISTRIBUTIONS Biochemistry & Molecular Biology Chemistry Biochemical Research Methods Analytical |
description |
Long-chain alkenones (LCAs) and alkenoates (LCEs) are highly valuable biomarkers for paleotemperature reconstructions. A major problem, however, for accurate quantification of these compounds using gas chromatography (GC) is co-elution with steryl ethers, wax esters, saturated ketones and other numerous mid-polarity compounds frequently encountered in marginal marine and lake sediments. Co-elution during GC separation is prevalent, particularly if the full homologous series of alkenones and alkenoates are to be analyzed. Taking advantage of the presence of two or more double bonds in LCAs and LCEs, the conventional silica gel impregnated with silver nitrate has previously been used to remove co-eluting compounds for LCAs. However, this conventional argentation chromatography is hampered by the extreme instability of silver nitrate, poor reproducibility, low recovery and short lifetime. Here we demonstrate a highly efficient flash chromatographic approach based on silver thiolate chromatographic material (AgTCM) that overcomes the shortcomings of the traditional argentation chromatography and allows repeated sample preparation (up to 62 samples in one test) with little loss in separation efficiency. AgTCM selectively extracts LCAs and LCEs and effectively eliminates co-eluting compounds including steryl ethers and wax esters for the subsequent gas chromatography (GC) analysis. This new method, therefore, allows low-cost and high-throughput sample preparation for comprehensive quantification of the full homologous series of LCAs and LCEs in marine and lake sediments. (C) 2019 Elsevier B.V. All rights reserved. |
format |
Report |
author |
Wang, Li Longo, William M. Dillon, James T. Zhao, Jiaju Zheng, Yinsui Moros, Matthias Huang, Yongsong |
author_facet |
Wang, Li Longo, William M. Dillon, James T. Zhao, Jiaju Zheng, Yinsui Moros, Matthias Huang, Yongsong |
author_sort |
Wang, Li |
title |
An efficient approach to eliminate steryl ethers and miscellaneous esters/ketones for gas chromatographic analysis of alkenones and alkenoates |
title_short |
An efficient approach to eliminate steryl ethers and miscellaneous esters/ketones for gas chromatographic analysis of alkenones and alkenoates |
title_full |
An efficient approach to eliminate steryl ethers and miscellaneous esters/ketones for gas chromatographic analysis of alkenones and alkenoates |
title_fullStr |
An efficient approach to eliminate steryl ethers and miscellaneous esters/ketones for gas chromatographic analysis of alkenones and alkenoates |
title_full_unstemmed |
An efficient approach to eliminate steryl ethers and miscellaneous esters/ketones for gas chromatographic analysis of alkenones and alkenoates |
title_sort |
efficient approach to eliminate steryl ethers and miscellaneous esters/ketones for gas chromatographic analysis of alkenones and alkenoates |
publisher |
ELSEVIER SCIENCE BV |
publishDate |
2019 |
url |
http://ir.ieecas.cn/handle/361006/13849 http://ir.ieecas.cn/handle/361006/13850 https://doi.org/10.1016/j.chroma.2019.02.064 |
genre |
Nordic Seas |
genre_facet |
Nordic Seas |
op_relation |
JOURNAL OF CHROMATOGRAPHY A http://ir.ieecas.cn/handle/361006/13849 http://ir.ieecas.cn/handle/361006/13850 doi:10.1016/j.chroma.2019.02.064 |
op_doi |
https://doi.org/10.1016/j.chroma.2019.02.064 |
container_title |
Journal of Chromatography A |
container_volume |
1596 |
container_start_page |
175 |
op_container_end_page |
182 |
_version_ |
1768392082984009728 |