Functional characterization of a group II interferon, IFNc in the perciform fish, Nile tilapia (Oreochromis niloticus)
Interferons are a family of class II alpha-helical cytokines playing vital roles in antiviral immune response, and little information is available to date regarding the interferon system of tilapia. In this study, a type I IFN gene, named On-IFNc, was identified in Nile tilapia, Oreochromis niloticu...
Published in: | Fish & Shellfish Immunology |
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Online Access: | http://ir.ihb.ac.cn/handle/342005/38892 https://doi.org/10.1016/j.fsi.2020.06.036 |
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ftchinacadsciihb:oai:ir.ihb.ac.cn:342005/38892 2023-05-15T15:32:38+02:00 Functional characterization of a group II interferon, IFNc in the perciform fish, Nile tilapia (Oreochromis niloticus) Gan, Zhen Cheng, Jun Chen, Shannan Laghari, Zubair Ahmed Hou, Jing Xia, Liqun Lu, Yishan Nie, Pin 2020-10-01 http://ir.ihb.ac.cn/handle/342005/38892 https://doi.org/10.1016/j.fsi.2020.06.036 英语 eng ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD FISH & SHELLFISH IMMUNOLOGY http://ir.ihb.ac.cn/handle/342005/38892 doi:10.1016/j.fsi.2020.06.036 Oreochromis niloticus Type I interferon Interferon-stimulated genes Tyrosine phosphorylation Antiviral activity Fisheries Immunology Marine & Freshwater Biology Veterinary Sciences KIDNEY NECROSIS VIRUS ATLANTIC SALMON MOLECULAR CHARACTERIZATION INFECTIOUS SPLEEN IMMUNE-RESPONSE RAINBOW-TROUT EXPRESSION GENES IDENTIFICATION DISTINCT 期刊论文 2020 ftchinacadsciihb https://doi.org/10.1016/j.fsi.2020.06.036 2020-12-04T01:07:43Z Interferons are a family of class II alpha-helical cytokines playing vital roles in antiviral immune response, and little information is available to date regarding the interferon system of tilapia. In this study, a type I IFN gene, named On-IFNc, was identified in Nile tilapia, Oreochromis niloticus. The predicted protein of On-IFNc contains several structural features known in type I IFNs, and On-IFNc was clustered together with the known IFNc in fish into a separated Glade in the phylogenetic tree. On-IFNc gene was constitutively expressed in all tissues examined, with the highest expression level observed in liver, and was rapidly induced in all organs/tissues tested following the stimulation of poly(I:C). In addition, recombinant On-IFNc has been proven to markedly induce the expression of the antiviral effectors, Mx and viperin, the signalling components, STAT1, STAT2, and IRF9, and the transcription factors, IRF3 and IRF7, as well as the tyrosine phosphorylation of STAT1 and STAT2 in fish cells. Furthermore, recombinant On-IFNc has been proven to possess antiviral activity against ISKNV. The present study thus contributes to a better understanding of the functional properties of the type I IFN system in tilapia. Report Atlantic salmon Institute of Hydrobiology, Chinese Academy of Sciences: IHB OpenIR Fish & Shellfish Immunology 105 86 94 |
institution |
Open Polar |
collection |
Institute of Hydrobiology, Chinese Academy of Sciences: IHB OpenIR |
op_collection_id |
ftchinacadsciihb |
language |
English |
topic |
Oreochromis niloticus Type I interferon Interferon-stimulated genes Tyrosine phosphorylation Antiviral activity Fisheries Immunology Marine & Freshwater Biology Veterinary Sciences KIDNEY NECROSIS VIRUS ATLANTIC SALMON MOLECULAR CHARACTERIZATION INFECTIOUS SPLEEN IMMUNE-RESPONSE RAINBOW-TROUT EXPRESSION GENES IDENTIFICATION DISTINCT |
spellingShingle |
Oreochromis niloticus Type I interferon Interferon-stimulated genes Tyrosine phosphorylation Antiviral activity Fisheries Immunology Marine & Freshwater Biology Veterinary Sciences KIDNEY NECROSIS VIRUS ATLANTIC SALMON MOLECULAR CHARACTERIZATION INFECTIOUS SPLEEN IMMUNE-RESPONSE RAINBOW-TROUT EXPRESSION GENES IDENTIFICATION DISTINCT Gan, Zhen Cheng, Jun Chen, Shannan Laghari, Zubair Ahmed Hou, Jing Xia, Liqun Lu, Yishan Nie, Pin Functional characterization of a group II interferon, IFNc in the perciform fish, Nile tilapia (Oreochromis niloticus) |
topic_facet |
Oreochromis niloticus Type I interferon Interferon-stimulated genes Tyrosine phosphorylation Antiviral activity Fisheries Immunology Marine & Freshwater Biology Veterinary Sciences KIDNEY NECROSIS VIRUS ATLANTIC SALMON MOLECULAR CHARACTERIZATION INFECTIOUS SPLEEN IMMUNE-RESPONSE RAINBOW-TROUT EXPRESSION GENES IDENTIFICATION DISTINCT |
description |
Interferons are a family of class II alpha-helical cytokines playing vital roles in antiviral immune response, and little information is available to date regarding the interferon system of tilapia. In this study, a type I IFN gene, named On-IFNc, was identified in Nile tilapia, Oreochromis niloticus. The predicted protein of On-IFNc contains several structural features known in type I IFNs, and On-IFNc was clustered together with the known IFNc in fish into a separated Glade in the phylogenetic tree. On-IFNc gene was constitutively expressed in all tissues examined, with the highest expression level observed in liver, and was rapidly induced in all organs/tissues tested following the stimulation of poly(I:C). In addition, recombinant On-IFNc has been proven to markedly induce the expression of the antiviral effectors, Mx and viperin, the signalling components, STAT1, STAT2, and IRF9, and the transcription factors, IRF3 and IRF7, as well as the tyrosine phosphorylation of STAT1 and STAT2 in fish cells. Furthermore, recombinant On-IFNc has been proven to possess antiviral activity against ISKNV. The present study thus contributes to a better understanding of the functional properties of the type I IFN system in tilapia. |
format |
Report |
author |
Gan, Zhen Cheng, Jun Chen, Shannan Laghari, Zubair Ahmed Hou, Jing Xia, Liqun Lu, Yishan Nie, Pin |
author_facet |
Gan, Zhen Cheng, Jun Chen, Shannan Laghari, Zubair Ahmed Hou, Jing Xia, Liqun Lu, Yishan Nie, Pin |
author_sort |
Gan, Zhen |
title |
Functional characterization of a group II interferon, IFNc in the perciform fish, Nile tilapia (Oreochromis niloticus) |
title_short |
Functional characterization of a group II interferon, IFNc in the perciform fish, Nile tilapia (Oreochromis niloticus) |
title_full |
Functional characterization of a group II interferon, IFNc in the perciform fish, Nile tilapia (Oreochromis niloticus) |
title_fullStr |
Functional characterization of a group II interferon, IFNc in the perciform fish, Nile tilapia (Oreochromis niloticus) |
title_full_unstemmed |
Functional characterization of a group II interferon, IFNc in the perciform fish, Nile tilapia (Oreochromis niloticus) |
title_sort |
functional characterization of a group ii interferon, ifnc in the perciform fish, nile tilapia (oreochromis niloticus) |
publisher |
ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD |
publishDate |
2020 |
url |
http://ir.ihb.ac.cn/handle/342005/38892 https://doi.org/10.1016/j.fsi.2020.06.036 |
genre |
Atlantic salmon |
genre_facet |
Atlantic salmon |
op_relation |
FISH & SHELLFISH IMMUNOLOGY http://ir.ihb.ac.cn/handle/342005/38892 doi:10.1016/j.fsi.2020.06.036 |
op_doi |
https://doi.org/10.1016/j.fsi.2020.06.036 |
container_title |
Fish & Shellfish Immunology |
container_volume |
105 |
container_start_page |
86 |
op_container_end_page |
94 |
_version_ |
1766363132035334144 |