Functional characterization of a group II interferon, IFNc in the perciform fish, Nile tilapia (Oreochromis niloticus)

Interferons are a family of class II alpha-helical cytokines playing vital roles in antiviral immune response, and little information is available to date regarding the interferon system of tilapia. In this study, a type I IFN gene, named On-IFNc, was identified in Nile tilapia, Oreochromis niloticu...

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Bibliographic Details
Published in:Fish & Shellfish Immunology
Main Authors: Gan, Zhen, Cheng, Jun, Chen, Shannan, Laghari, Zubair Ahmed, Hou, Jing, Xia, Liqun, Lu, Yishan, Nie, Pin
Format: Report
Language:English
Published: ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD 2020
Subjects:
Oreochromis niloticus
Type I interferon
Interferon-stimulated genes
Tyrosine phosphorylation
Antiviral activity
Fisheries
Immunology
Marine & Freshwater Biology
Veterinary Sciences
KIDNEY NECROSIS VIRUS
ATLANTIC SALMON
MOLECULAR CHARACTERIZATION
INFECTIOUS SPLEEN
IMMUNE-RESPONSE
RAINBOW-TROUT
EXPRESSION
GENES
IDENTIFICATION
DISTINCT
Atlantic salmon
Online Access:http://ir.ihb.ac.cn/handle/342005/38892
https://doi.org/10.1016/j.fsi.2020.06.036
Description
Summary:Interferons are a family of class II alpha-helical cytokines playing vital roles in antiviral immune response, and little information is available to date regarding the interferon system of tilapia. In this study, a type I IFN gene, named On-IFNc, was identified in Nile tilapia, Oreochromis niloticus. The predicted protein of On-IFNc contains several structural features known in type I IFNs, and On-IFNc was clustered together with the known IFNc in fish into a separated Glade in the phylogenetic tree. On-IFNc gene was constitutively expressed in all tissues examined, with the highest expression level observed in liver, and was rapidly induced in all organs/tissues tested following the stimulation of poly(I:C). In addition, recombinant On-IFNc has been proven to markedly induce the expression of the antiviral effectors, Mx and viperin, the signalling components, STAT1, STAT2, and IRF9, and the transcription factors, IRF3 and IRF7, as well as the tyrosine phosphorylation of STAT1 and STAT2 in fish cells. Furthermore, recombinant On-IFNc has been proven to possess antiviral activity against ISKNV. The present study thus contributes to a better understanding of the functional properties of the type I IFN system in tilapia.

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