Pathogenic characterization ofAeromonas salmonicidasubsp.masoucidaturbot isolate from China
Aeromonas salmonicidais a gram-negative bacterium that is the causative agent of furunculosis. AnA. salmonicidastrain was isolated from diseased turbot (Scophthalmus maximus) with the sign of furunculosis from North China. Based onvapAgene, the strain was further classified asA. salmonicidasubsp.mas...
Published in: | Journal of Fish Diseases |
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ftchinacadsciihb:oai:ir.ihb.ac.cn:342005/36883 2023-05-15T18:15:50+02:00 Pathogenic characterization ofAeromonas salmonicidasubsp.masoucidaturbot isolate from China Wang, Ping Jie, Li He, Tian Tian Li, Nan Mo, Zhao Lan Nie, Pin Xie, Hai Xia 2020-07-27 http://ir.ihb.ac.cn/handle/342005/36882 http://ir.ihb.ac.cn/handle/342005/36883 https://doi.org/10.1111/jfd.13224 英语 eng WILEY JOURNAL OF FISH DISEASES http://ir.ihb.ac.cn/handle/342005/36882 http://ir.ihb.ac.cn/handle/342005/36883 doi:10.1111/jfd.13224 Aeromonas salmonicidasubsp masoucida apoptosis identification invasion virulence Fisheries Marine & Freshwater Biology Veterinary Sciences ATYPICAL AEROMONAS-SALMONICIDA III SECRETION SYSTEM SUBSP-SALMONICIDA EFFECTOR PROTEIN INFECTION GENES 期刊论文 2020 ftchinacadsciihb https://doi.org/10.1111/jfd.13224 2020-11-20T01:07:24Z Aeromonas salmonicidais a gram-negative bacterium that is the causative agent of furunculosis. AnA. salmonicidastrain was isolated from diseased turbot (Scophthalmus maximus) with the sign of furunculosis from North China. Based onvapAgene, the strain was further classified asA. salmonicidasubsp.masoucidaRZ6S-1. Culturing RZ6S-1 strain at high temperature (28 degrees C) obtained the virulence attenuated strain RZ6S. Genome sequence comparison between the two strains revealed the loss of the type IV secretion system (T4SS) and type III secretion system (T3SS) from the native plasmid pAsmB-1 and pAsmC-1 of wild-type strain RZ6S-1, respectively. Further study demonstrated that the wild-type strain RZ6S-1, but not its derivative mutant RZ6S, can stimulate apoptosis. Elevated protein level of cleaved caspase-3 was detected from epithelioma papulosum cyprinid (EPC) cells infected with wild-type strain RZ6S-1 as compared with that infected with RZ6S strain. Meanwhile, the invasion of the mutant strain RZ6S was about 17-fold higher than the wild-type strain RZ6S-1, suggesting that some protein(s) fromA. salmonicidasubsp.masoucidaRZ6S-1 suppress its invasion. The RZ6S mutant strain was attenuated, since its LD(50)is over 10,000 times higher compared to the wild-type strain as revealed in the turbot infection model. Report Scophthalmus maximus Turbot Institute of Hydrobiology, Chinese Academy of Sciences: IHB OpenIR Journal of Fish Diseases 43 10 1145 1154 |
institution |
Open Polar |
collection |
Institute of Hydrobiology, Chinese Academy of Sciences: IHB OpenIR |
op_collection_id |
ftchinacadsciihb |
language |
English |
topic |
Aeromonas salmonicidasubsp masoucida apoptosis identification invasion virulence Fisheries Marine & Freshwater Biology Veterinary Sciences ATYPICAL AEROMONAS-SALMONICIDA III SECRETION SYSTEM SUBSP-SALMONICIDA EFFECTOR PROTEIN INFECTION GENES |
spellingShingle |
Aeromonas salmonicidasubsp masoucida apoptosis identification invasion virulence Fisheries Marine & Freshwater Biology Veterinary Sciences ATYPICAL AEROMONAS-SALMONICIDA III SECRETION SYSTEM SUBSP-SALMONICIDA EFFECTOR PROTEIN INFECTION GENES Wang, Ping Jie, Li He, Tian Tian Li, Nan Mo, Zhao Lan Nie, Pin Xie, Hai Xia Pathogenic characterization ofAeromonas salmonicidasubsp.masoucidaturbot isolate from China |
topic_facet |
Aeromonas salmonicidasubsp masoucida apoptosis identification invasion virulence Fisheries Marine & Freshwater Biology Veterinary Sciences ATYPICAL AEROMONAS-SALMONICIDA III SECRETION SYSTEM SUBSP-SALMONICIDA EFFECTOR PROTEIN INFECTION GENES |
description |
Aeromonas salmonicidais a gram-negative bacterium that is the causative agent of furunculosis. AnA. salmonicidastrain was isolated from diseased turbot (Scophthalmus maximus) with the sign of furunculosis from North China. Based onvapAgene, the strain was further classified asA. salmonicidasubsp.masoucidaRZ6S-1. Culturing RZ6S-1 strain at high temperature (28 degrees C) obtained the virulence attenuated strain RZ6S. Genome sequence comparison between the two strains revealed the loss of the type IV secretion system (T4SS) and type III secretion system (T3SS) from the native plasmid pAsmB-1 and pAsmC-1 of wild-type strain RZ6S-1, respectively. Further study demonstrated that the wild-type strain RZ6S-1, but not its derivative mutant RZ6S, can stimulate apoptosis. Elevated protein level of cleaved caspase-3 was detected from epithelioma papulosum cyprinid (EPC) cells infected with wild-type strain RZ6S-1 as compared with that infected with RZ6S strain. Meanwhile, the invasion of the mutant strain RZ6S was about 17-fold higher than the wild-type strain RZ6S-1, suggesting that some protein(s) fromA. salmonicidasubsp.masoucidaRZ6S-1 suppress its invasion. The RZ6S mutant strain was attenuated, since its LD(50)is over 10,000 times higher compared to the wild-type strain as revealed in the turbot infection model. |
format |
Report |
author |
Wang, Ping Jie, Li He, Tian Tian Li, Nan Mo, Zhao Lan Nie, Pin Xie, Hai Xia |
author_facet |
Wang, Ping Jie, Li He, Tian Tian Li, Nan Mo, Zhao Lan Nie, Pin Xie, Hai Xia |
author_sort |
Wang, Ping |
title |
Pathogenic characterization ofAeromonas salmonicidasubsp.masoucidaturbot isolate from China |
title_short |
Pathogenic characterization ofAeromonas salmonicidasubsp.masoucidaturbot isolate from China |
title_full |
Pathogenic characterization ofAeromonas salmonicidasubsp.masoucidaturbot isolate from China |
title_fullStr |
Pathogenic characterization ofAeromonas salmonicidasubsp.masoucidaturbot isolate from China |
title_full_unstemmed |
Pathogenic characterization ofAeromonas salmonicidasubsp.masoucidaturbot isolate from China |
title_sort |
pathogenic characterization ofaeromonas salmonicidasubsp.masoucidaturbot isolate from china |
publisher |
WILEY |
publishDate |
2020 |
url |
http://ir.ihb.ac.cn/handle/342005/36882 http://ir.ihb.ac.cn/handle/342005/36883 https://doi.org/10.1111/jfd.13224 |
genre |
Scophthalmus maximus Turbot |
genre_facet |
Scophthalmus maximus Turbot |
op_relation |
JOURNAL OF FISH DISEASES http://ir.ihb.ac.cn/handle/342005/36882 http://ir.ihb.ac.cn/handle/342005/36883 doi:10.1111/jfd.13224 |
op_doi |
https://doi.org/10.1111/jfd.13224 |
container_title |
Journal of Fish Diseases |
container_volume |
43 |
container_issue |
10 |
container_start_page |
1145 |
op_container_end_page |
1154 |
_version_ |
1766189061101322240 |