Transcriptomic analysis of oligochaete immune responses to myxosporeans infection: Branchiura sowerbyi infected with Myxobolus cultus
The Myxozoa are endoparasites characterized by a two-host life cycle that typically involves invertebrates and vertebrates as definitive and intermediate hosts, respectively. However, little is known about invertebrate-myxosporean interactions, particularly about patterns of host immune defense. We...
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ftchinacadsciihb:oai:ir.ihb.ac.cn:342005/35486 2023-05-15T18:15:52+02:00 Transcriptomic analysis of oligochaete immune responses to myxosporeans infection: Branchiura sowerbyi infected with Myxobolus cultus Zhao, Yuanli Liu, Xinhua Xi, Bingwen Zhang, Qianqian Li, Aihua Zhang, Jinyong 2020 http://ir.ihb.ac.cn/handle/342005/35486 https://doi.org/10.1016/j.jip.2019.107283 英语 eng ACADEMIC PRESS INC ELSEVIER SCIENCE JOURNAL OF INVERTEBRATE PATHOLOGY http://ir.ihb.ac.cn/handle/342005/35486 doi:10.1016/j.jip.2019.107283 Branchiura sowerbyi Myxobolus cultus Innate immune response Transcriptomics Invertebrate-myxosporean interaction Zoology SHRIMP LITOPENAEUS-VANNAMEI SCOPHTHALMUS-MAXIMUS CYSTEINE-PROTEASES CAUSATIVE AGENT INNATE IMMUNITY LIFE-CYCLE GENE CEREBRALIS MYXOZOA INSIGHTS 期刊论文 2020 ftchinacadsciihb https://doi.org/10.1016/j.jip.2019.107283 2020-05-15T00:06:24Z The Myxozoa are endoparasites characterized by a two-host life cycle that typically involves invertebrates and vertebrates as definitive and intermediate hosts, respectively. However, little is known about invertebrate-myxosporean interactions, particularly about patterns of host immune defense. We used RNA-sequencing to identify genes that are possibly involved in the immune responses of the oligochaete Branchiura sowerbyi naturally infected with Myxobolus cilium. De novo assembly of the B. sowerbyi transcriptome yielded 119,031 unigenes, with an average length of 896 bp and an N50 length of 1754 bp. Comparative transcriptome analysis revealed 4059 differentially expressed genes (DEGs) between M. cultus-infected and uninfected B. sowerbyi groups, including 3802 upregulated genes and 257 downregulated genes. Among the B. sowerbyi immune factors implicated in the responses to M. cultus infection, DEGs related to lectins, ubiquitin-mediated proteolysis, phagocytosis, oxidative-antioxidative responses, proteases, and protease inhibitors were upregulated. The expression of some immune-related molecules such as calmodulin, heat shock proteins, antimicrobial peptides, lysenin, and serum amyoid A protein were also significantly upregulated. The expression patterns of 14 immune-related DEGs identified by RNA-seq were validated by quantitative real-time polymerase chain reaction. This study is the first attempt to characterize the B. sowerbyi transcriptome and identify immune-related molecules possibly associated with M. cultus infection. It is also the first report of invertebrate host-myxosporean interactions at the transcriptomic level. Our results will facilitate the elucidation of adaptive evolution mechanisms of myxosporean parasites in the definitive host and the genetic basis for differences in resistance of invertebrate hosts of different genotypes to a myxosporean species. Report Scophthalmus maximus Institute of Hydrobiology, Chinese Academy of Sciences: IHB OpenIR Journal of Invertebrate Pathology 169 107283 |
institution |
Open Polar |
collection |
Institute of Hydrobiology, Chinese Academy of Sciences: IHB OpenIR |
op_collection_id |
ftchinacadsciihb |
language |
English |
topic |
Branchiura sowerbyi Myxobolus cultus Innate immune response Transcriptomics Invertebrate-myxosporean interaction Zoology SHRIMP LITOPENAEUS-VANNAMEI SCOPHTHALMUS-MAXIMUS CYSTEINE-PROTEASES CAUSATIVE AGENT INNATE IMMUNITY LIFE-CYCLE GENE CEREBRALIS MYXOZOA INSIGHTS |
spellingShingle |
Branchiura sowerbyi Myxobolus cultus Innate immune response Transcriptomics Invertebrate-myxosporean interaction Zoology SHRIMP LITOPENAEUS-VANNAMEI SCOPHTHALMUS-MAXIMUS CYSTEINE-PROTEASES CAUSATIVE AGENT INNATE IMMUNITY LIFE-CYCLE GENE CEREBRALIS MYXOZOA INSIGHTS Zhao, Yuanli Liu, Xinhua Xi, Bingwen Zhang, Qianqian Li, Aihua Zhang, Jinyong Transcriptomic analysis of oligochaete immune responses to myxosporeans infection: Branchiura sowerbyi infected with Myxobolus cultus |
topic_facet |
Branchiura sowerbyi Myxobolus cultus Innate immune response Transcriptomics Invertebrate-myxosporean interaction Zoology SHRIMP LITOPENAEUS-VANNAMEI SCOPHTHALMUS-MAXIMUS CYSTEINE-PROTEASES CAUSATIVE AGENT INNATE IMMUNITY LIFE-CYCLE GENE CEREBRALIS MYXOZOA INSIGHTS |
description |
The Myxozoa are endoparasites characterized by a two-host life cycle that typically involves invertebrates and vertebrates as definitive and intermediate hosts, respectively. However, little is known about invertebrate-myxosporean interactions, particularly about patterns of host immune defense. We used RNA-sequencing to identify genes that are possibly involved in the immune responses of the oligochaete Branchiura sowerbyi naturally infected with Myxobolus cilium. De novo assembly of the B. sowerbyi transcriptome yielded 119,031 unigenes, with an average length of 896 bp and an N50 length of 1754 bp. Comparative transcriptome analysis revealed 4059 differentially expressed genes (DEGs) between M. cultus-infected and uninfected B. sowerbyi groups, including 3802 upregulated genes and 257 downregulated genes. Among the B. sowerbyi immune factors implicated in the responses to M. cultus infection, DEGs related to lectins, ubiquitin-mediated proteolysis, phagocytosis, oxidative-antioxidative responses, proteases, and protease inhibitors were upregulated. The expression of some immune-related molecules such as calmodulin, heat shock proteins, antimicrobial peptides, lysenin, and serum amyoid A protein were also significantly upregulated. The expression patterns of 14 immune-related DEGs identified by RNA-seq were validated by quantitative real-time polymerase chain reaction. This study is the first attempt to characterize the B. sowerbyi transcriptome and identify immune-related molecules possibly associated with M. cultus infection. It is also the first report of invertebrate host-myxosporean interactions at the transcriptomic level. Our results will facilitate the elucidation of adaptive evolution mechanisms of myxosporean parasites in the definitive host and the genetic basis for differences in resistance of invertebrate hosts of different genotypes to a myxosporean species. |
format |
Report |
author |
Zhao, Yuanli Liu, Xinhua Xi, Bingwen Zhang, Qianqian Li, Aihua Zhang, Jinyong |
author_facet |
Zhao, Yuanli Liu, Xinhua Xi, Bingwen Zhang, Qianqian Li, Aihua Zhang, Jinyong |
author_sort |
Zhao, Yuanli |
title |
Transcriptomic analysis of oligochaete immune responses to myxosporeans infection: Branchiura sowerbyi infected with Myxobolus cultus |
title_short |
Transcriptomic analysis of oligochaete immune responses to myxosporeans infection: Branchiura sowerbyi infected with Myxobolus cultus |
title_full |
Transcriptomic analysis of oligochaete immune responses to myxosporeans infection: Branchiura sowerbyi infected with Myxobolus cultus |
title_fullStr |
Transcriptomic analysis of oligochaete immune responses to myxosporeans infection: Branchiura sowerbyi infected with Myxobolus cultus |
title_full_unstemmed |
Transcriptomic analysis of oligochaete immune responses to myxosporeans infection: Branchiura sowerbyi infected with Myxobolus cultus |
title_sort |
transcriptomic analysis of oligochaete immune responses to myxosporeans infection: branchiura sowerbyi infected with myxobolus cultus |
publisher |
ACADEMIC PRESS INC ELSEVIER SCIENCE |
publishDate |
2020 |
url |
http://ir.ihb.ac.cn/handle/342005/35486 https://doi.org/10.1016/j.jip.2019.107283 |
genre |
Scophthalmus maximus |
genre_facet |
Scophthalmus maximus |
op_relation |
JOURNAL OF INVERTEBRATE PATHOLOGY http://ir.ihb.ac.cn/handle/342005/35486 doi:10.1016/j.jip.2019.107283 |
op_doi |
https://doi.org/10.1016/j.jip.2019.107283 |
container_title |
Journal of Invertebrate Pathology |
container_volume |
169 |
container_start_page |
107283 |
_version_ |
1766189106315919360 |