Dietary low or excess levels of lipids reduced growth performance, and impaired immune function and structure of head kidney, spleen and skin in young grass carp (Ctenopharyngodon idella) under the infection of Aeromonas hydrophila
Our study explored the effect of dietary lipids on growth and immunity and structure (head kidney, spleen and skin) of young grass, carp (Ctenopharyngodon idella). A total of 540 young grass carp with an average initial weight of 261.41 +/- 0.53 g were fed diets containing six graded levels of lipid...
Published in: | Fish & Shellfish Immunology |
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ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD
2016
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Online Access: | http://ir.ihb.ac.cn/handle/342005/34688 https://doi.org/10.1016/j.fsi.2016.03.163 |
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ftchinacadsciihb:oai:ir.ihb.ac.cn:342005/34688 2023-05-15T18:10:03+02:00 Dietary low or excess levels of lipids reduced growth performance, and impaired immune function and structure of head kidney, spleen and skin in young grass carp (Ctenopharyngodon idella) under the infection of Aeromonas hydrophila Ni, Pei-Jun Jiang, Wei-Dan Wu, Pei Liu, Yang Kuang, Sheng-Yao Tang, Ling Tang, Wu-Neng Zhang, Yong-An Zhou, Xiao-Qiu Feng, Lin 2016-08-01 http://ir.ihb.ac.cn/handle/342005/34688 https://doi.org/10.1016/j.fsi.2016.03.163 英语 eng ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD FISH & SHELLFISH IMMUNOLOGY http://ir.ihb.ac.cn/handle/342005/34688 doi:10.1016/j.fsi.2016.03.163 Ctenopharyngodon idella Lipids Immune organs Bacterial challenge Structure integrity Immune function Fisheries Immunology Marine & Freshwater Biology Veterinary Sciences NF-KAPPA-B TIGHT JUNCTION PROTEINS SALMON SALMO-SALAR ANTIOXIDANT ENZYME-ACTIVITIES SOLEA-SENEGALENSIS JUVENILES INTESTINAL MUCOSAL IMMUNE MESSENGER-RNA EXPRESSION OXIDATIVE STRESS CYPRINUS-CARPIO GENE-EXPRESSION 期刊论文 2016 ftchinacadsciihb https://doi.org/10.1016/j.fsi.2016.03.163 2020-01-17T01:05:56Z Our study explored the effect of dietary lipids on growth and immunity and structure (head kidney, spleen and skin) of young grass, carp (Ctenopharyngodon idella). A total of 540 young grass carp with an average initial weight of 261.41 +/- 0.53 g were fed diets containing six graded levels of lipids at 5.9 -80.1 WIT diet for 8 weeks. After that, a challenge trial was conducted by injection of Aeromonas hydrophila over 2 weeks. The results indicated that compared with optimal lipids supplementation, low and excess levels of lipids down-regulated the mRNA levels of antimicrobial peptides, anti-inflammatory cytokines, inhibitor of kappa B alpha (I kappa B alpha) and ribosomal p70S6 kinase (S6K1), and up-regulated pro-inflammatory cytokines, nuclear factor kappa B p65 (NF-kappa B p65), NF-kappa B c-Rel (not p52), I kappa B kinase alpha (IKK alpha), IKK beta, IKK gamma, and eIF4E-binding protein (4EBP) mRNA levels in the head kidney and spleen of young grass carp (P < 0.05). Low or excess levels of lipids also increased reactive oxygen species (ROS) production and malondialdehyde (MDA) and protein carbonyl (PC) contents, reduced the activities of antioxidant enzymes (P < 0.05), down-regulate the relative mRNA levels of antioxidant enzymes and NF-E2-related factor 2 (Nrf2), and up-regulated the expression levels of Kelch-like ECH-associating protein la (Keap1a) and Keap1b in the head kidney and spleen. In addition, low or excess levels of lipids down regulated the mRNA levels of B-cell lymphoma-2 (Bcl-2) and inhibitor of apoptosis protein (IAP) in the head kidney and spleen, whereas up-regulated the mRNA levels of apoptotic protease activating factor-1 (Apaf-1), caspase 3, 7, 8 and 9 mRNA levels in the head kidney and spleen and Fas ligand (FasL) mRNA levels in the spleen of young grass carp, suggesting that low or excess levels of lipids could decrease the head kidney and spleen immune function, induce oxidative damage and apoptosis and impair antioxidant system of young grass carp. At last, low or excess levels of lipids also impaired the immune function and structure in the skin of young grass carp. Based on the quadratic regression analysis for PWG, skin haemorrhage and lesions morbidity and IgM content, the dietary lipids requirements for young grass carp were estimated to be 43.7, 60.2, 55.0 and 52.1 g/kg diet, respectively. (C) 2016 Published by Elsevier Ltd. Report Salmo salar Institute of Hydrobiology, Chinese Academy of Sciences: IHB OpenIR Fish & Shellfish Immunology 55 28 47 |
institution |
Open Polar |
collection |
Institute of Hydrobiology, Chinese Academy of Sciences: IHB OpenIR |
op_collection_id |
ftchinacadsciihb |
language |
English |
topic |
Ctenopharyngodon idella Lipids Immune organs Bacterial challenge Structure integrity Immune function Fisheries Immunology Marine & Freshwater Biology Veterinary Sciences NF-KAPPA-B TIGHT JUNCTION PROTEINS SALMON SALMO-SALAR ANTIOXIDANT ENZYME-ACTIVITIES SOLEA-SENEGALENSIS JUVENILES INTESTINAL MUCOSAL IMMUNE MESSENGER-RNA EXPRESSION OXIDATIVE STRESS CYPRINUS-CARPIO GENE-EXPRESSION |
spellingShingle |
Ctenopharyngodon idella Lipids Immune organs Bacterial challenge Structure integrity Immune function Fisheries Immunology Marine & Freshwater Biology Veterinary Sciences NF-KAPPA-B TIGHT JUNCTION PROTEINS SALMON SALMO-SALAR ANTIOXIDANT ENZYME-ACTIVITIES SOLEA-SENEGALENSIS JUVENILES INTESTINAL MUCOSAL IMMUNE MESSENGER-RNA EXPRESSION OXIDATIVE STRESS CYPRINUS-CARPIO GENE-EXPRESSION Ni, Pei-Jun Jiang, Wei-Dan Wu, Pei Liu, Yang Kuang, Sheng-Yao Tang, Ling Tang, Wu-Neng Zhang, Yong-An Zhou, Xiao-Qiu Feng, Lin Dietary low or excess levels of lipids reduced growth performance, and impaired immune function and structure of head kidney, spleen and skin in young grass carp (Ctenopharyngodon idella) under the infection of Aeromonas hydrophila |
topic_facet |
Ctenopharyngodon idella Lipids Immune organs Bacterial challenge Structure integrity Immune function Fisheries Immunology Marine & Freshwater Biology Veterinary Sciences NF-KAPPA-B TIGHT JUNCTION PROTEINS SALMON SALMO-SALAR ANTIOXIDANT ENZYME-ACTIVITIES SOLEA-SENEGALENSIS JUVENILES INTESTINAL MUCOSAL IMMUNE MESSENGER-RNA EXPRESSION OXIDATIVE STRESS CYPRINUS-CARPIO GENE-EXPRESSION |
description |
Our study explored the effect of dietary lipids on growth and immunity and structure (head kidney, spleen and skin) of young grass, carp (Ctenopharyngodon idella). A total of 540 young grass carp with an average initial weight of 261.41 +/- 0.53 g were fed diets containing six graded levels of lipids at 5.9 -80.1 WIT diet for 8 weeks. After that, a challenge trial was conducted by injection of Aeromonas hydrophila over 2 weeks. The results indicated that compared with optimal lipids supplementation, low and excess levels of lipids down-regulated the mRNA levels of antimicrobial peptides, anti-inflammatory cytokines, inhibitor of kappa B alpha (I kappa B alpha) and ribosomal p70S6 kinase (S6K1), and up-regulated pro-inflammatory cytokines, nuclear factor kappa B p65 (NF-kappa B p65), NF-kappa B c-Rel (not p52), I kappa B kinase alpha (IKK alpha), IKK beta, IKK gamma, and eIF4E-binding protein (4EBP) mRNA levels in the head kidney and spleen of young grass carp (P < 0.05). Low or excess levels of lipids also increased reactive oxygen species (ROS) production and malondialdehyde (MDA) and protein carbonyl (PC) contents, reduced the activities of antioxidant enzymes (P < 0.05), down-regulate the relative mRNA levels of antioxidant enzymes and NF-E2-related factor 2 (Nrf2), and up-regulated the expression levels of Kelch-like ECH-associating protein la (Keap1a) and Keap1b in the head kidney and spleen. In addition, low or excess levels of lipids down regulated the mRNA levels of B-cell lymphoma-2 (Bcl-2) and inhibitor of apoptosis protein (IAP) in the head kidney and spleen, whereas up-regulated the mRNA levels of apoptotic protease activating factor-1 (Apaf-1), caspase 3, 7, 8 and 9 mRNA levels in the head kidney and spleen and Fas ligand (FasL) mRNA levels in the spleen of young grass carp, suggesting that low or excess levels of lipids could decrease the head kidney and spleen immune function, induce oxidative damage and apoptosis and impair antioxidant system of young grass carp. At last, low or excess levels of lipids also impaired the immune function and structure in the skin of young grass carp. Based on the quadratic regression analysis for PWG, skin haemorrhage and lesions morbidity and IgM content, the dietary lipids requirements for young grass carp were estimated to be 43.7, 60.2, 55.0 and 52.1 g/kg diet, respectively. (C) 2016 Published by Elsevier Ltd. |
format |
Report |
author |
Ni, Pei-Jun Jiang, Wei-Dan Wu, Pei Liu, Yang Kuang, Sheng-Yao Tang, Ling Tang, Wu-Neng Zhang, Yong-An Zhou, Xiao-Qiu Feng, Lin |
author_facet |
Ni, Pei-Jun Jiang, Wei-Dan Wu, Pei Liu, Yang Kuang, Sheng-Yao Tang, Ling Tang, Wu-Neng Zhang, Yong-An Zhou, Xiao-Qiu Feng, Lin |
author_sort |
Ni, Pei-Jun |
title |
Dietary low or excess levels of lipids reduced growth performance, and impaired immune function and structure of head kidney, spleen and skin in young grass carp (Ctenopharyngodon idella) under the infection of Aeromonas hydrophila |
title_short |
Dietary low or excess levels of lipids reduced growth performance, and impaired immune function and structure of head kidney, spleen and skin in young grass carp (Ctenopharyngodon idella) under the infection of Aeromonas hydrophila |
title_full |
Dietary low or excess levels of lipids reduced growth performance, and impaired immune function and structure of head kidney, spleen and skin in young grass carp (Ctenopharyngodon idella) under the infection of Aeromonas hydrophila |
title_fullStr |
Dietary low or excess levels of lipids reduced growth performance, and impaired immune function and structure of head kidney, spleen and skin in young grass carp (Ctenopharyngodon idella) under the infection of Aeromonas hydrophila |
title_full_unstemmed |
Dietary low or excess levels of lipids reduced growth performance, and impaired immune function and structure of head kidney, spleen and skin in young grass carp (Ctenopharyngodon idella) under the infection of Aeromonas hydrophila |
title_sort |
dietary low or excess levels of lipids reduced growth performance, and impaired immune function and structure of head kidney, spleen and skin in young grass carp (ctenopharyngodon idella) under the infection of aeromonas hydrophila |
publisher |
ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD |
publishDate |
2016 |
url |
http://ir.ihb.ac.cn/handle/342005/34688 https://doi.org/10.1016/j.fsi.2016.03.163 |
genre |
Salmo salar |
genre_facet |
Salmo salar |
op_relation |
FISH & SHELLFISH IMMUNOLOGY http://ir.ihb.ac.cn/handle/342005/34688 doi:10.1016/j.fsi.2016.03.163 |
op_doi |
https://doi.org/10.1016/j.fsi.2016.03.163 |
container_title |
Fish & Shellfish Immunology |
container_volume |
55 |
container_start_page |
28 |
op_container_end_page |
47 |
_version_ |
1766182795303976960 |