Dietary vitamin C deficiency depressed the gill physical barriers and immune barriers referring to Nrf2, apoptosis, MLCK, NF-kappa B and TOR signaling in grass carp (Ctenopharyngodon idella) under infection of Flavobacterium columnare
This study explored the effects of vitamin C on the physical barriers and immune barriers, and relative mRNA levels of signaling molecules in the gill of grass carp (Ctenopharyngodon idella) under infection of Flavobacterium columnare. The results indicated that compared with optimal vitamin C suppl...
Published in: | Fish & Shellfish Immunology |
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Main Authors: | , , , , , , , , , , |
Format: | Report |
Language: | English |
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ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD
2016
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Subjects: | |
Online Access: | http://ir.ihb.ac.cn/handle/342005/34107 http://ir.ihb.ac.cn/handle/342005/34108 https://doi.org/10.1016/j.fsi.2016.09.029 |
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ftchinacadsciihb:oai:ir.ihb.ac.cn:342005/34108 |
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record_format |
openpolar |
institution |
Open Polar |
collection |
Institute of Hydrobiology, Chinese Academy of Sciences: IHB OpenIR |
op_collection_id |
ftchinacadsciihb |
language |
English |
topic |
Vitamin C Physical barrier Immune barrier Gill Grass carp (Ctenopharyngodon idella) Fisheries Immunology Marine & Freshwater Biology Veterinary Sciences MESSENGER-RNA EXPRESSION TIGHT JUNCTION PROTEINS LIGHT-CHAIN KINASE SALMON SALMO-SALAR ANTIOXIDANT STATUS OXIDATIVE STRESS ATLANTIC SALMON JUVENILE JIAN VAR. JIAN MEGALOBRAMA-AMBLYCEPHALA |
spellingShingle |
Vitamin C Physical barrier Immune barrier Gill Grass carp (Ctenopharyngodon idella) Fisheries Immunology Marine & Freshwater Biology Veterinary Sciences MESSENGER-RNA EXPRESSION TIGHT JUNCTION PROTEINS LIGHT-CHAIN KINASE SALMON SALMO-SALAR ANTIOXIDANT STATUS OXIDATIVE STRESS ATLANTIC SALMON JUVENILE JIAN VAR. JIAN MEGALOBRAMA-AMBLYCEPHALA Xu, Hui-Jun Jiang, Wei-Dan Feng, Lin Liu, Yang Wu, Pei Jiang, Jun Kuang, Sheng-Yao Tang, Ling Tang, Wu-Neng Zhang, Yong-An Zhou, Xiao-Qiu Dietary vitamin C deficiency depressed the gill physical barriers and immune barriers referring to Nrf2, apoptosis, MLCK, NF-kappa B and TOR signaling in grass carp (Ctenopharyngodon idella) under infection of Flavobacterium columnare |
topic_facet |
Vitamin C Physical barrier Immune barrier Gill Grass carp (Ctenopharyngodon idella) Fisheries Immunology Marine & Freshwater Biology Veterinary Sciences MESSENGER-RNA EXPRESSION TIGHT JUNCTION PROTEINS LIGHT-CHAIN KINASE SALMON SALMO-SALAR ANTIOXIDANT STATUS OXIDATIVE STRESS ATLANTIC SALMON JUVENILE JIAN VAR. JIAN MEGALOBRAMA-AMBLYCEPHALA |
description |
This study explored the effects of vitamin C on the physical barriers and immune barriers, and relative mRNA levels of signaling molecules in the gill of grass carp (Ctenopharyngodon idella) under infection of Flavobacterium columnare. The results indicated that compared with optimal vitamin C supplementation, vitamin C deficiency (2.9 mg/kg diet) (1) increased reactive oxygen species, malondialdehyde and protein carbonyl (PC) contents (P < 0.05), decreased the copper/zinc superoxide dismutase, manganese superoxide dismutase, catalase, glutathione peroxidase and glutathione reductase activities and mRNA levels (P < 0.05), and glutathione and vitamin C contents (P < 0.05), down-regulated NF-E2-related factor 2 mRNA level (P < 0.05), and up-regulated Kelch-like ECH-associating protein (Keap) 1a (rather than Keap1b) mRNA level (P < 0.05) in the gill of grass carp under infection of F. columnare, suggesting that vitamin C deficiency induced oxidative injury in fish gill; (2) up-regulated caspase-3, -7, -8, -9, Fas ligand, B-cell lymphoma protein 2 associated X protein, apoptotic protease activating factor-1 mRNA levels (P < 0.05), and down-regulated inhibitor of apoptosis protein and B-cell lymphoma-2 (rather than myeloid cell leukemia-1) mRNA level (P < 0.05) in the gill of grass carp under infection of F columnare, suggesting that vitamin C deficiency aggravated cell apoptosis in fish gill; (3) up-regulated pore-forming TJs Claudin-12, 15a, -15b, and related signaling molecules myosin light chain kinase, p38 mitogen-activated protein kinase (rather than c-jun N-terminal kinases) mRNA levels (P < 0.05), and down regulated barrier-forming TJs Occludin, zonula occludens (ZO) 1, ZO-2, Claudin-c, -3c, -7a, -7b mRNA levels (P < 0.05) in the gill of grass carp under infection of F. columnare, suggesting that vitamin C deficiency disrupted tight junctional complexes in fish gill; (4) decreased lysozyme and acid phosphatase (ACP) activities, and complement 3 (C3), C4 and IgM contents (P < 0.05), down-regulated the mRNA levels of antimicrobial peptides liver expressed antimicrobial peptide (LEAP) 2A, LEAP-2B, Hepcidin, defensin mRNA levels (P < 0.05) in the gill of grass carp under infection of E columnare, suggesting that vitamin C deficiency decrease fish gill immune function; (5) down-regulated the mRNA levels of anti-inflammatory cytokines-related factors interleukin 10 (IL-10), IL-11, transforming growth factor (TGF) beta 1, TGF-beta 2, inhibitor of kappa Ba and eIF4E-binding protein 1 (4E-BP1) (rather than 4E-BP2) (P < 0.05), and up regulated pro-inflammatory cytokines-related factors interferon gamma 2, IL-beta, IL-6, IL-8, IL-12 P35, IL-12 P40, nuclear factor kappa B (NF-kappa B) p65 (rather than NF-kappa B p52), I kappa B kinases (IKK) (only IKK alpha and IKK gamma), target of rapamycin and ribosomal protein S6 kinase 1 mRNA levels (P < 0.05) in the gill of grass carp under infection of F columnare, suggesting that vitamin C deficiency aggravated fish gill inflammation. In conclusion, vitamin C deficiency disrupted physical barriers and immune barriers, and regulated relative mRNA levels of signaling molecules in fish gill. The vitamin C requirement for against gill rot morbidity of grass carp (264-1031 g) was estimated to be 156.0 mg/kg diet. In addition, based on the gill biochemical indices (antioxidant indices MDA, PC and vitamin C contents, and immune indices LA and ACP activity) the vitamin C requirements for grass carp (264-1031 g) were estimated to be 116.8, 156.6, 110.8, 57.8 and 134.9 mg/kg diet, respectively. (C) 2016 Elsevier Ltd. All rights reserved. |
format |
Report |
author |
Xu, Hui-Jun Jiang, Wei-Dan Feng, Lin Liu, Yang Wu, Pei Jiang, Jun Kuang, Sheng-Yao Tang, Ling Tang, Wu-Neng Zhang, Yong-An Zhou, Xiao-Qiu |
author_facet |
Xu, Hui-Jun Jiang, Wei-Dan Feng, Lin Liu, Yang Wu, Pei Jiang, Jun Kuang, Sheng-Yao Tang, Ling Tang, Wu-Neng Zhang, Yong-An Zhou, Xiao-Qiu |
author_sort |
Xu, Hui-Jun |
title |
Dietary vitamin C deficiency depressed the gill physical barriers and immune barriers referring to Nrf2, apoptosis, MLCK, NF-kappa B and TOR signaling in grass carp (Ctenopharyngodon idella) under infection of Flavobacterium columnare |
title_short |
Dietary vitamin C deficiency depressed the gill physical barriers and immune barriers referring to Nrf2, apoptosis, MLCK, NF-kappa B and TOR signaling in grass carp (Ctenopharyngodon idella) under infection of Flavobacterium columnare |
title_full |
Dietary vitamin C deficiency depressed the gill physical barriers and immune barriers referring to Nrf2, apoptosis, MLCK, NF-kappa B and TOR signaling in grass carp (Ctenopharyngodon idella) under infection of Flavobacterium columnare |
title_fullStr |
Dietary vitamin C deficiency depressed the gill physical barriers and immune barriers referring to Nrf2, apoptosis, MLCK, NF-kappa B and TOR signaling in grass carp (Ctenopharyngodon idella) under infection of Flavobacterium columnare |
title_full_unstemmed |
Dietary vitamin C deficiency depressed the gill physical barriers and immune barriers referring to Nrf2, apoptosis, MLCK, NF-kappa B and TOR signaling in grass carp (Ctenopharyngodon idella) under infection of Flavobacterium columnare |
title_sort |
dietary vitamin c deficiency depressed the gill physical barriers and immune barriers referring to nrf2, apoptosis, mlck, nf-kappa b and tor signaling in grass carp (ctenopharyngodon idella) under infection of flavobacterium columnare |
publisher |
ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD |
publishDate |
2016 |
url |
http://ir.ihb.ac.cn/handle/342005/34107 http://ir.ihb.ac.cn/handle/342005/34108 https://doi.org/10.1016/j.fsi.2016.09.029 |
genre |
Atlantic salmon Salmo salar |
genre_facet |
Atlantic salmon Salmo salar |
op_relation |
FISH & SHELLFISH IMMUNOLOGY http://ir.ihb.ac.cn/handle/342005/34107 http://ir.ihb.ac.cn/handle/342005/34108 doi:10.1016/j.fsi.2016.09.029 |
op_doi |
https://doi.org/10.1016/j.fsi.2016.09.029 |
container_title |
Fish & Shellfish Immunology |
container_volume |
58 |
container_start_page |
177 |
op_container_end_page |
192 |
_version_ |
1766363637754101760 |
spelling |
ftchinacadsciihb:oai:ir.ihb.ac.cn:342005/34108 2023-05-15T15:33:10+02:00 Dietary vitamin C deficiency depressed the gill physical barriers and immune barriers referring to Nrf2, apoptosis, MLCK, NF-kappa B and TOR signaling in grass carp (Ctenopharyngodon idella) under infection of Flavobacterium columnare Xu, Hui-Jun Jiang, Wei-Dan Feng, Lin Liu, Yang Wu, Pei Jiang, Jun Kuang, Sheng-Yao Tang, Ling Tang, Wu-Neng Zhang, Yong-An Zhou, Xiao-Qiu 2016-11-01 http://ir.ihb.ac.cn/handle/342005/34107 http://ir.ihb.ac.cn/handle/342005/34108 https://doi.org/10.1016/j.fsi.2016.09.029 英语 eng ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD FISH & SHELLFISH IMMUNOLOGY http://ir.ihb.ac.cn/handle/342005/34107 http://ir.ihb.ac.cn/handle/342005/34108 doi:10.1016/j.fsi.2016.09.029 Vitamin C Physical barrier Immune barrier Gill Grass carp (Ctenopharyngodon idella) Fisheries Immunology Marine & Freshwater Biology Veterinary Sciences MESSENGER-RNA EXPRESSION TIGHT JUNCTION PROTEINS LIGHT-CHAIN KINASE SALMON SALMO-SALAR ANTIOXIDANT STATUS OXIDATIVE STRESS ATLANTIC SALMON JUVENILE JIAN VAR. JIAN MEGALOBRAMA-AMBLYCEPHALA 期刊论文 2016 ftchinacadsciihb https://doi.org/10.1016/j.fsi.2016.09.029 2019-11-15T01:05:11Z This study explored the effects of vitamin C on the physical barriers and immune barriers, and relative mRNA levels of signaling molecules in the gill of grass carp (Ctenopharyngodon idella) under infection of Flavobacterium columnare. The results indicated that compared with optimal vitamin C supplementation, vitamin C deficiency (2.9 mg/kg diet) (1) increased reactive oxygen species, malondialdehyde and protein carbonyl (PC) contents (P < 0.05), decreased the copper/zinc superoxide dismutase, manganese superoxide dismutase, catalase, glutathione peroxidase and glutathione reductase activities and mRNA levels (P < 0.05), and glutathione and vitamin C contents (P < 0.05), down-regulated NF-E2-related factor 2 mRNA level (P < 0.05), and up-regulated Kelch-like ECH-associating protein (Keap) 1a (rather than Keap1b) mRNA level (P < 0.05) in the gill of grass carp under infection of F. columnare, suggesting that vitamin C deficiency induced oxidative injury in fish gill; (2) up-regulated caspase-3, -7, -8, -9, Fas ligand, B-cell lymphoma protein 2 associated X protein, apoptotic protease activating factor-1 mRNA levels (P < 0.05), and down-regulated inhibitor of apoptosis protein and B-cell lymphoma-2 (rather than myeloid cell leukemia-1) mRNA level (P < 0.05) in the gill of grass carp under infection of F columnare, suggesting that vitamin C deficiency aggravated cell apoptosis in fish gill; (3) up-regulated pore-forming TJs Claudin-12, 15a, -15b, and related signaling molecules myosin light chain kinase, p38 mitogen-activated protein kinase (rather than c-jun N-terminal kinases) mRNA levels (P < 0.05), and down regulated barrier-forming TJs Occludin, zonula occludens (ZO) 1, ZO-2, Claudin-c, -3c, -7a, -7b mRNA levels (P < 0.05) in the gill of grass carp under infection of F. columnare, suggesting that vitamin C deficiency disrupted tight junctional complexes in fish gill; (4) decreased lysozyme and acid phosphatase (ACP) activities, and complement 3 (C3), C4 and IgM contents (P < 0.05), down-regulated the mRNA levels of antimicrobial peptides liver expressed antimicrobial peptide (LEAP) 2A, LEAP-2B, Hepcidin, defensin mRNA levels (P < 0.05) in the gill of grass carp under infection of E columnare, suggesting that vitamin C deficiency decrease fish gill immune function; (5) down-regulated the mRNA levels of anti-inflammatory cytokines-related factors interleukin 10 (IL-10), IL-11, transforming growth factor (TGF) beta 1, TGF-beta 2, inhibitor of kappa Ba and eIF4E-binding protein 1 (4E-BP1) (rather than 4E-BP2) (P < 0.05), and up regulated pro-inflammatory cytokines-related factors interferon gamma 2, IL-beta, IL-6, IL-8, IL-12 P35, IL-12 P40, nuclear factor kappa B (NF-kappa B) p65 (rather than NF-kappa B p52), I kappa B kinases (IKK) (only IKK alpha and IKK gamma), target of rapamycin and ribosomal protein S6 kinase 1 mRNA levels (P < 0.05) in the gill of grass carp under infection of F columnare, suggesting that vitamin C deficiency aggravated fish gill inflammation. In conclusion, vitamin C deficiency disrupted physical barriers and immune barriers, and regulated relative mRNA levels of signaling molecules in fish gill. The vitamin C requirement for against gill rot morbidity of grass carp (264-1031 g) was estimated to be 156.0 mg/kg diet. In addition, based on the gill biochemical indices (antioxidant indices MDA, PC and vitamin C contents, and immune indices LA and ACP activity) the vitamin C requirements for grass carp (264-1031 g) were estimated to be 116.8, 156.6, 110.8, 57.8 and 134.9 mg/kg diet, respectively. (C) 2016 Elsevier Ltd. All rights reserved. Report Atlantic salmon Salmo salar Institute of Hydrobiology, Chinese Academy of Sciences: IHB OpenIR Fish & Shellfish Immunology 58 177 192 |