Effect of substitution of dietary fish meal by soybean meal on different sizes of gibel carp (Carassius auratus gibelio): digestive enzyme gene expressions and activities, and intestinal and hepatic histology
A 7-week growth trial was conducted to evaluate the effects of dietary soybean meal (SBM) on digestive enzyme activity of intestinal mucosa, mRNA levels of digestive enzymes in hepatopancreas, and the mid-intestinal and hepatopancreas histology of gibel carp CAS III (Carassius auratus gibelio). Four...
Published in: | Aquaculture Nutrition |
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WILEY-BLACKWELL
2017
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Online Access: | http://ir.ihb.ac.cn/handle/342005/33115 https://doi.org/10.1111/anu.12375 |
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ftchinacadsciihb:oai:ir.ihb.ac.cn:342005/33115 2023-05-15T15:32:50+02:00 Effect of substitution of dietary fish meal by soybean meal on different sizes of gibel carp (Carassius auratus gibelio): digestive enzyme gene expressions and activities, and intestinal and hepatic histology Liu, H. Jin, J. Zhu, X. Han, D. Yang, Y. Xie, S. 2017-02-01 http://ir.ihb.ac.cn/handle/342005/33115 https://doi.org/10.1111/anu.12375 英语 eng WILEY-BLACKWELL AQUACULTURE NUTRITION http://ir.ihb.ac.cn/handle/342005/33115 doi:10.1111/anu.12375 digestive enzyme gene expression gibel carp histology soybean meal Fisheries SALMON SALMO-SALAR TROUT ONCORHYNCHUS-MYKISS PLANT PROTEIN-SOURCES COD GADUS-MORHUA BASS DICENTRARCHUS-LABRAX SEABREAM SPARUS-AURATA BRUSH-BORDER MEMBRANE GILTHEAD SEA BREAM ATLANTIC SALMON RAINBOW-TROUT 期刊论文 2017 ftchinacadsciihb https://doi.org/10.1111/anu.12375 2019-10-18T00:04:21Z A 7-week growth trial was conducted to evaluate the effects of dietary soybean meal (SBM) on digestive enzyme activity of intestinal mucosa, mRNA levels of digestive enzymes in hepatopancreas, and the mid-intestinal and hepatopancreas histology of gibel carp CAS III (Carassius auratus gibelio). Four different growth phases of gibel carp (initial body weight: fry, 0.8 g; juvenile, 5.0 g; 1-year-old, 62.7 g; and broodstock, 135.6 g) were tested. Seven isonitrogenous and iso-energetic diets were formulated to contain different SBM replacement levels (0%, 20%, 40%, 60%, 80% and 100% of dietary fish meal protein), and another diet (SBMAA) contained all SBM protein and supplied crystalline amino acids. The results showed that the activities of mid-intestine trypsin, a-amylase and gamma glutamyl transpeptidase reduced with increased dietary SBM, while the chymotrypsin activity increased first and then decreased. The ultrastructures of intestinal epithelial cells and hepatopancreas cells in fry and broodstock fish were distinctly affected by 200 g kg(-1) dietary SBM. Supplementation of dietary amino acid to the highest replacement groups was not sufficient to improve digestive and absorptive capacities and growth performance. Gibel carp may be adapted to dietary SBM through increase in gene expression of hepatopancreas digestive enzymes and has potential to utilize proceeded SBM as feedstuffs. Report Atlantic salmon Gadus morhua Salmo salar Institute of Hydrobiology, Chinese Academy of Sciences: IHB OpenIR Aquaculture Nutrition 23 1 129 147 |
institution |
Open Polar |
collection |
Institute of Hydrobiology, Chinese Academy of Sciences: IHB OpenIR |
op_collection_id |
ftchinacadsciihb |
language |
English |
topic |
digestive enzyme gene expression gibel carp histology soybean meal Fisheries SALMON SALMO-SALAR TROUT ONCORHYNCHUS-MYKISS PLANT PROTEIN-SOURCES COD GADUS-MORHUA BASS DICENTRARCHUS-LABRAX SEABREAM SPARUS-AURATA BRUSH-BORDER MEMBRANE GILTHEAD SEA BREAM ATLANTIC SALMON RAINBOW-TROUT |
spellingShingle |
digestive enzyme gene expression gibel carp histology soybean meal Fisheries SALMON SALMO-SALAR TROUT ONCORHYNCHUS-MYKISS PLANT PROTEIN-SOURCES COD GADUS-MORHUA BASS DICENTRARCHUS-LABRAX SEABREAM SPARUS-AURATA BRUSH-BORDER MEMBRANE GILTHEAD SEA BREAM ATLANTIC SALMON RAINBOW-TROUT Liu, H. Jin, J. Zhu, X. Han, D. Yang, Y. Xie, S. Effect of substitution of dietary fish meal by soybean meal on different sizes of gibel carp (Carassius auratus gibelio): digestive enzyme gene expressions and activities, and intestinal and hepatic histology |
topic_facet |
digestive enzyme gene expression gibel carp histology soybean meal Fisheries SALMON SALMO-SALAR TROUT ONCORHYNCHUS-MYKISS PLANT PROTEIN-SOURCES COD GADUS-MORHUA BASS DICENTRARCHUS-LABRAX SEABREAM SPARUS-AURATA BRUSH-BORDER MEMBRANE GILTHEAD SEA BREAM ATLANTIC SALMON RAINBOW-TROUT |
description |
A 7-week growth trial was conducted to evaluate the effects of dietary soybean meal (SBM) on digestive enzyme activity of intestinal mucosa, mRNA levels of digestive enzymes in hepatopancreas, and the mid-intestinal and hepatopancreas histology of gibel carp CAS III (Carassius auratus gibelio). Four different growth phases of gibel carp (initial body weight: fry, 0.8 g; juvenile, 5.0 g; 1-year-old, 62.7 g; and broodstock, 135.6 g) were tested. Seven isonitrogenous and iso-energetic diets were formulated to contain different SBM replacement levels (0%, 20%, 40%, 60%, 80% and 100% of dietary fish meal protein), and another diet (SBMAA) contained all SBM protein and supplied crystalline amino acids. The results showed that the activities of mid-intestine trypsin, a-amylase and gamma glutamyl transpeptidase reduced with increased dietary SBM, while the chymotrypsin activity increased first and then decreased. The ultrastructures of intestinal epithelial cells and hepatopancreas cells in fry and broodstock fish were distinctly affected by 200 g kg(-1) dietary SBM. Supplementation of dietary amino acid to the highest replacement groups was not sufficient to improve digestive and absorptive capacities and growth performance. Gibel carp may be adapted to dietary SBM through increase in gene expression of hepatopancreas digestive enzymes and has potential to utilize proceeded SBM as feedstuffs. |
format |
Report |
author |
Liu, H. Jin, J. Zhu, X. Han, D. Yang, Y. Xie, S. |
author_facet |
Liu, H. Jin, J. Zhu, X. Han, D. Yang, Y. Xie, S. |
author_sort |
Liu, H. |
title |
Effect of substitution of dietary fish meal by soybean meal on different sizes of gibel carp (Carassius auratus gibelio): digestive enzyme gene expressions and activities, and intestinal and hepatic histology |
title_short |
Effect of substitution of dietary fish meal by soybean meal on different sizes of gibel carp (Carassius auratus gibelio): digestive enzyme gene expressions and activities, and intestinal and hepatic histology |
title_full |
Effect of substitution of dietary fish meal by soybean meal on different sizes of gibel carp (Carassius auratus gibelio): digestive enzyme gene expressions and activities, and intestinal and hepatic histology |
title_fullStr |
Effect of substitution of dietary fish meal by soybean meal on different sizes of gibel carp (Carassius auratus gibelio): digestive enzyme gene expressions and activities, and intestinal and hepatic histology |
title_full_unstemmed |
Effect of substitution of dietary fish meal by soybean meal on different sizes of gibel carp (Carassius auratus gibelio): digestive enzyme gene expressions and activities, and intestinal and hepatic histology |
title_sort |
effect of substitution of dietary fish meal by soybean meal on different sizes of gibel carp (carassius auratus gibelio): digestive enzyme gene expressions and activities, and intestinal and hepatic histology |
publisher |
WILEY-BLACKWELL |
publishDate |
2017 |
url |
http://ir.ihb.ac.cn/handle/342005/33115 https://doi.org/10.1111/anu.12375 |
genre |
Atlantic salmon Gadus morhua Salmo salar |
genre_facet |
Atlantic salmon Gadus morhua Salmo salar |
op_relation |
AQUACULTURE NUTRITION http://ir.ihb.ac.cn/handle/342005/33115 doi:10.1111/anu.12375 |
op_doi |
https://doi.org/10.1111/anu.12375 |
container_title |
Aquaculture Nutrition |
container_volume |
23 |
container_issue |
1 |
container_start_page |
129 |
op_container_end_page |
147 |
_version_ |
1766363316255457280 |