Vitamin E deficiency depressed fish growth, disease resistance, and the immunity and structural integrity of immune organs in grass carp (Ctenopharyngodon idella): Referring to NF-kappa B, TOR and Nrf2 signaling
This study investigated the effects of dietary vitamin E on growth, disease resistance and the immunity and structural integrity of head kidney, spleen and skin in grass carp (Ctenopharyngodon idella). The fish were fed six diets containing graded levels of vitamin E (0, 45, 90, 135, 180 and 225 mg/...
Published in: | Fish & Shellfish Immunology |
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Language: | English |
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ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD
2017
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Online Access: | http://ir.ihb.ac.cn/handle/342005/33090 https://doi.org/10.1016/j.fsi.2016.11.044 |
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ftchinacadsciihb:oai:ir.ihb.ac.cn:342005/33090 2023-05-15T18:15:54+02:00 Vitamin E deficiency depressed fish growth, disease resistance, and the immunity and structural integrity of immune organs in grass carp (Ctenopharyngodon idella): Referring to NF-kappa B, TOR and Nrf2 signaling Pan, Jia-Hong Feng, Lin Jiang, Wei-Dan Wu, Pei Kuang, Sheng-Yao Tang, Ling Zhang, Yong-An Zhou, Xiao-Qiu Liu, Yang 2017 http://ir.ihb.ac.cn/handle/342005/33090 https://doi.org/10.1016/j.fsi.2016.11.044 英语 eng ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD FISH & SHELLFISH IMMUNOLOGY http://ir.ihb.ac.cn/handle/342005/33090 doi:10.1016/j.fsi.2016.11.044 Vitamin E Grass carp (Ctenopharyngodon idella) Immunity Antioxidant capacity Apoptosis Immune organs Fisheries Immunology Marine & Freshwater Biology Veterinary Sciences TURBOT SCOPHTHALMUS-MAXIMUS PRO-INFLAMMATORY CYTOKINES SPLEEN FOLLOWING INFECTION METHIONINE HYDROXY ANALOG HEAD KIDNEY ANTIOXIDANT STATUS ALPHA-TOCOPHEROL CYPRINUS-CARPIO GENE-EXPRESSION JUVENILE JIAN 期刊论文 2017 ftchinacadsciihb https://doi.org/10.1016/j.fsi.2016.11.044 2019-10-18T00:04:21Z This study investigated the effects of dietary vitamin E on growth, disease resistance and the immunity and structural integrity of head kidney, spleen and skin in grass carp (Ctenopharyngodon idella). The fish were fed six diets containing graded levels of vitamin E (0, 45, 90, 135, 180 and 225 mg/kg diet) for 10 weeks. Subsequently, a challenge test was conducted by injection of Aeromonas hydrophila. The results showed that compared with optimal vitamin E supplementation, vitamin E deficiency caused depressed growth, poor survival rates and increased skin lesion morbidity in grass carp. Meanwhile, vitamin E deficiency decreased lysozyme and acid phosphatase activities, complement component 3 and complement component 4 contents in the head kidney, spleen and skin of grass carp (P < 0.05). Moreover, vitamin E deficiency down-regulated antimicrobial peptides (Hepcidin, liver-expressed antimicrobial peptide-2A,-2B, beta-defensin), IL-10, TGF beta 1, I kappa B alpha, TOR and S6K1 mRNA levels (P < 0.05) and up-regulated IL-1 beta, IL-6, IL-8, IFN-gamma 2 and TNF alpha, NF-kappa B p65, IKK alpha, IKK beta and 4EBP1 (not in the head kidney) mRNA levels (P < 0.05). In addition, vitamin E deficiency caused oxidative damage, decreased superoxide dismutase (SOD), glutathione peroxidase (GPx), catalase (CAT) and glutathione reductase (GR) activities, and down regulated the mRNA levels of antioxidant enzymes and signaling molecules Nrf2 (P < 0.05). Vitamin E deficiency also induced apoptosis by up-regulating capase-2,-3,-7, and-8 mRNA levels in the head kidney, spleen and skin of grass carp. In conclusion, this study indicated that dietary vitamin E deficiency depressed fish growth, impaired the immune function and disturbed the structural integrity of the head kidney, spleen and skin in grass carp, but optimal vitamin E supplementation can reverse those negative effects in fish. The optimal vitamin E requirements for young grass carp (266.39-1026.63 g) to achieve optimal growth performance and disease resistance based on the percent weight gain (PWG) and skin lesion morbidity were estimated to be 116.2 and 130.9 mg/kg diet, respectively. Meanwhile, based on immune indicator (LA activity in the head kidney) and antioxidant indicator (protection of spleen against MDA), the optimal vitamin E requirements for young grass carp were estimated to be 123.8 and 136.4 mg/kg diet, respectively. (C) 2016 Elsevier Ltd. All rights reserved. Report Scophthalmus maximus Turbot Institute of Hydrobiology, Chinese Academy of Sciences: IHB OpenIR Fish & Shellfish Immunology 60 219 236 |
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Open Polar |
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Institute of Hydrobiology, Chinese Academy of Sciences: IHB OpenIR |
op_collection_id |
ftchinacadsciihb |
language |
English |
topic |
Vitamin E Grass carp (Ctenopharyngodon idella) Immunity Antioxidant capacity Apoptosis Immune organs Fisheries Immunology Marine & Freshwater Biology Veterinary Sciences TURBOT SCOPHTHALMUS-MAXIMUS PRO-INFLAMMATORY CYTOKINES SPLEEN FOLLOWING INFECTION METHIONINE HYDROXY ANALOG HEAD KIDNEY ANTIOXIDANT STATUS ALPHA-TOCOPHEROL CYPRINUS-CARPIO GENE-EXPRESSION JUVENILE JIAN |
spellingShingle |
Vitamin E Grass carp (Ctenopharyngodon idella) Immunity Antioxidant capacity Apoptosis Immune organs Fisheries Immunology Marine & Freshwater Biology Veterinary Sciences TURBOT SCOPHTHALMUS-MAXIMUS PRO-INFLAMMATORY CYTOKINES SPLEEN FOLLOWING INFECTION METHIONINE HYDROXY ANALOG HEAD KIDNEY ANTIOXIDANT STATUS ALPHA-TOCOPHEROL CYPRINUS-CARPIO GENE-EXPRESSION JUVENILE JIAN Pan, Jia-Hong Feng, Lin Jiang, Wei-Dan Wu, Pei Kuang, Sheng-Yao Tang, Ling Zhang, Yong-An Zhou, Xiao-Qiu Liu, Yang Vitamin E deficiency depressed fish growth, disease resistance, and the immunity and structural integrity of immune organs in grass carp (Ctenopharyngodon idella): Referring to NF-kappa B, TOR and Nrf2 signaling |
topic_facet |
Vitamin E Grass carp (Ctenopharyngodon idella) Immunity Antioxidant capacity Apoptosis Immune organs Fisheries Immunology Marine & Freshwater Biology Veterinary Sciences TURBOT SCOPHTHALMUS-MAXIMUS PRO-INFLAMMATORY CYTOKINES SPLEEN FOLLOWING INFECTION METHIONINE HYDROXY ANALOG HEAD KIDNEY ANTIOXIDANT STATUS ALPHA-TOCOPHEROL CYPRINUS-CARPIO GENE-EXPRESSION JUVENILE JIAN |
description |
This study investigated the effects of dietary vitamin E on growth, disease resistance and the immunity and structural integrity of head kidney, spleen and skin in grass carp (Ctenopharyngodon idella). The fish were fed six diets containing graded levels of vitamin E (0, 45, 90, 135, 180 and 225 mg/kg diet) for 10 weeks. Subsequently, a challenge test was conducted by injection of Aeromonas hydrophila. The results showed that compared with optimal vitamin E supplementation, vitamin E deficiency caused depressed growth, poor survival rates and increased skin lesion morbidity in grass carp. Meanwhile, vitamin E deficiency decreased lysozyme and acid phosphatase activities, complement component 3 and complement component 4 contents in the head kidney, spleen and skin of grass carp (P < 0.05). Moreover, vitamin E deficiency down-regulated antimicrobial peptides (Hepcidin, liver-expressed antimicrobial peptide-2A,-2B, beta-defensin), IL-10, TGF beta 1, I kappa B alpha, TOR and S6K1 mRNA levels (P < 0.05) and up-regulated IL-1 beta, IL-6, IL-8, IFN-gamma 2 and TNF alpha, NF-kappa B p65, IKK alpha, IKK beta and 4EBP1 (not in the head kidney) mRNA levels (P < 0.05). In addition, vitamin E deficiency caused oxidative damage, decreased superoxide dismutase (SOD), glutathione peroxidase (GPx), catalase (CAT) and glutathione reductase (GR) activities, and down regulated the mRNA levels of antioxidant enzymes and signaling molecules Nrf2 (P < 0.05). Vitamin E deficiency also induced apoptosis by up-regulating capase-2,-3,-7, and-8 mRNA levels in the head kidney, spleen and skin of grass carp. In conclusion, this study indicated that dietary vitamin E deficiency depressed fish growth, impaired the immune function and disturbed the structural integrity of the head kidney, spleen and skin in grass carp, but optimal vitamin E supplementation can reverse those negative effects in fish. The optimal vitamin E requirements for young grass carp (266.39-1026.63 g) to achieve optimal growth performance and disease resistance based on the percent weight gain (PWG) and skin lesion morbidity were estimated to be 116.2 and 130.9 mg/kg diet, respectively. Meanwhile, based on immune indicator (LA activity in the head kidney) and antioxidant indicator (protection of spleen against MDA), the optimal vitamin E requirements for young grass carp were estimated to be 123.8 and 136.4 mg/kg diet, respectively. (C) 2016 Elsevier Ltd. All rights reserved. |
format |
Report |
author |
Pan, Jia-Hong Feng, Lin Jiang, Wei-Dan Wu, Pei Kuang, Sheng-Yao Tang, Ling Zhang, Yong-An Zhou, Xiao-Qiu Liu, Yang |
author_facet |
Pan, Jia-Hong Feng, Lin Jiang, Wei-Dan Wu, Pei Kuang, Sheng-Yao Tang, Ling Zhang, Yong-An Zhou, Xiao-Qiu Liu, Yang |
author_sort |
Pan, Jia-Hong |
title |
Vitamin E deficiency depressed fish growth, disease resistance, and the immunity and structural integrity of immune organs in grass carp (Ctenopharyngodon idella): Referring to NF-kappa B, TOR and Nrf2 signaling |
title_short |
Vitamin E deficiency depressed fish growth, disease resistance, and the immunity and structural integrity of immune organs in grass carp (Ctenopharyngodon idella): Referring to NF-kappa B, TOR and Nrf2 signaling |
title_full |
Vitamin E deficiency depressed fish growth, disease resistance, and the immunity and structural integrity of immune organs in grass carp (Ctenopharyngodon idella): Referring to NF-kappa B, TOR and Nrf2 signaling |
title_fullStr |
Vitamin E deficiency depressed fish growth, disease resistance, and the immunity and structural integrity of immune organs in grass carp (Ctenopharyngodon idella): Referring to NF-kappa B, TOR and Nrf2 signaling |
title_full_unstemmed |
Vitamin E deficiency depressed fish growth, disease resistance, and the immunity and structural integrity of immune organs in grass carp (Ctenopharyngodon idella): Referring to NF-kappa B, TOR and Nrf2 signaling |
title_sort |
vitamin e deficiency depressed fish growth, disease resistance, and the immunity and structural integrity of immune organs in grass carp (ctenopharyngodon idella): referring to nf-kappa b, tor and nrf2 signaling |
publisher |
ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD |
publishDate |
2017 |
url |
http://ir.ihb.ac.cn/handle/342005/33090 https://doi.org/10.1016/j.fsi.2016.11.044 |
genre |
Scophthalmus maximus Turbot |
genre_facet |
Scophthalmus maximus Turbot |
op_relation |
FISH & SHELLFISH IMMUNOLOGY http://ir.ihb.ac.cn/handle/342005/33090 doi:10.1016/j.fsi.2016.11.044 |
op_doi |
https://doi.org/10.1016/j.fsi.2016.11.044 |
container_title |
Fish & Shellfish Immunology |
container_volume |
60 |
container_start_page |
219 |
op_container_end_page |
236 |
_version_ |
1766189162083385344 |