Vitamin A deficiency suppresses fish immune function with differences in different intestinal segments: the role of transcriptional factor NF-kappa B and p38 mitogen-activated protein kinase signalling pathways
The present study investigated the effects of dietary vitamin A on immune function in the proximal intestine (PI), mid intestine (MI) and distal intestine (DI) of young grass carp (Ctenopharyngodon idella). Fish were fed graded levels of dietary vitamin A for 10 weeks, and then a challenge test usin...
Published in: | British Journal of Nutrition |
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Language: | English |
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CAMBRIDGE UNIV PRESS
2017
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Online Access: | http://ir.ihb.ac.cn/handle/342005/32973 http://ir.ihb.ac.cn/handle/342005/32974 https://doi.org/10.1017/S0007114516003342 |
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ftchinacadsciihb:oai:ir.ihb.ac.cn:342005/32974 2023-05-15T15:33:03+02:00 Vitamin A deficiency suppresses fish immune function with differences in different intestinal segments: the role of transcriptional factor NF-kappa B and p38 mitogen-activated protein kinase signalling pathways Zhang, Li Feng, Lin Jiang, Wei-Dan Liu, Yang Wu, Pei Kuang, Sheng-Yao Tang, Ling Tang, Wu-Neng Zhang, Yong-An Zhou, Xiao-Qiu 2017 http://ir.ihb.ac.cn/handle/342005/32973 http://ir.ihb.ac.cn/handle/342005/32974 https://doi.org/10.1017/S0007114516003342 英语 eng CAMBRIDGE UNIV PRESS BRITISH JOURNAL OF NUTRITION http://ir.ihb.ac.cn/handle/342005/32973 http://ir.ihb.ac.cn/handle/342005/32974 doi:10.1017/S0007114516003342 Vitamin A deficiency Intestinal segments Immune responses NF-kappa B canonical signalling p38 Mitogen-activated protein kinase Grass carp Nutrition & Dietetics CARP CTENOPHARYNGODON-IDELLA SALMON SALMO-SALAR TIGHT JUNCTION PROTEINS TILAPIA OREOCHROMIS-NILOTICUS JUVENILE JIAN DISEASE RESISTANCE ATLANTIC SALMON RETINOIC ACID VAR. JIAN 期刊论文 2017 ftchinacadsciihb https://doi.org/10.1017/S0007114516003342 2019-10-04T00:04:23Z The present study investigated the effects of dietary vitamin A on immune function in the proximal intestine (PI), mid intestine (MI) and distal intestine (DI) of young grass carp (Ctenopharyngodon idella). Fish were fed graded levels of dietary vitamin A for 10 weeks, and then a challenge test using an injection of Aeromonas hydrophila was conducted for 14 d. The results showed that, compared with the optimum vitamin A level, vitamin A deficiency significantly decreased fish growth performance, increased enteritis morbidity, decreased intestinal innate humoral immune response and aggravated intestinal inflammation. However, liver-expressed antimicrobial peptide 2A/B mRNA in the DI and IL-6, IL-17D, IL-10, transforming growth factor (TGF)-beta 1 and TGF-beta 2 mRNA in the PI were not affected by vitamin A levels. Meanwhile, vitamin A deficiency disturbed inflammatory cytokines in the PI, MI and DI, which might be partly linked to p38 mitogen-activated protein kinase (p38MAPK) signalling and NF-kappa B canonical signalling pathway (I kappa B kinase beta (IKK beta), IKK gamma, inhibitor of kappa B alpha, NF-kappa B p65 and c-Rel) rather than NF-kappa B non-canonical signalling pathway (NF-kappa B p52 and IKK alpha). However, the signalling molecules NF-kappa B p65 and p38MAPK did not participate in regulating cytokines in the PI. These results suggested that vitamin A deficiency decreased fish growth and impaired intestinal immune function, and that different immune responses in the PI, MI and DI were mediated partly by NF-kappa B canonical signalling and p38MAPK signalling pathways. On the basis of percentage of weight gain, to protect fish against enteritis morbidity and acid phosphatase activity, the optimum dietary vitamin A levels were estimated to be 0.664, 0.707 and 0.722 mg/kg, respectively. Report Atlantic salmon Salmo salar Institute of Hydrobiology, Chinese Academy of Sciences: IHB OpenIR British Journal of Nutrition 117 1 67 82 |
institution |
Open Polar |
collection |
Institute of Hydrobiology, Chinese Academy of Sciences: IHB OpenIR |
op_collection_id |
ftchinacadsciihb |
language |
English |
topic |
Vitamin A deficiency Intestinal segments Immune responses NF-kappa B canonical signalling p38 Mitogen-activated protein kinase Grass carp Nutrition & Dietetics CARP CTENOPHARYNGODON-IDELLA SALMON SALMO-SALAR TIGHT JUNCTION PROTEINS TILAPIA OREOCHROMIS-NILOTICUS JUVENILE JIAN DISEASE RESISTANCE ATLANTIC SALMON RETINOIC ACID VAR. JIAN |
spellingShingle |
Vitamin A deficiency Intestinal segments Immune responses NF-kappa B canonical signalling p38 Mitogen-activated protein kinase Grass carp Nutrition & Dietetics CARP CTENOPHARYNGODON-IDELLA SALMON SALMO-SALAR TIGHT JUNCTION PROTEINS TILAPIA OREOCHROMIS-NILOTICUS JUVENILE JIAN DISEASE RESISTANCE ATLANTIC SALMON RETINOIC ACID VAR. JIAN Zhang, Li Feng, Lin Jiang, Wei-Dan Liu, Yang Wu, Pei Kuang, Sheng-Yao Tang, Ling Tang, Wu-Neng Zhang, Yong-An Zhou, Xiao-Qiu Vitamin A deficiency suppresses fish immune function with differences in different intestinal segments: the role of transcriptional factor NF-kappa B and p38 mitogen-activated protein kinase signalling pathways |
topic_facet |
Vitamin A deficiency Intestinal segments Immune responses NF-kappa B canonical signalling p38 Mitogen-activated protein kinase Grass carp Nutrition & Dietetics CARP CTENOPHARYNGODON-IDELLA SALMON SALMO-SALAR TIGHT JUNCTION PROTEINS TILAPIA OREOCHROMIS-NILOTICUS JUVENILE JIAN DISEASE RESISTANCE ATLANTIC SALMON RETINOIC ACID VAR. JIAN |
description |
The present study investigated the effects of dietary vitamin A on immune function in the proximal intestine (PI), mid intestine (MI) and distal intestine (DI) of young grass carp (Ctenopharyngodon idella). Fish were fed graded levels of dietary vitamin A for 10 weeks, and then a challenge test using an injection of Aeromonas hydrophila was conducted for 14 d. The results showed that, compared with the optimum vitamin A level, vitamin A deficiency significantly decreased fish growth performance, increased enteritis morbidity, decreased intestinal innate humoral immune response and aggravated intestinal inflammation. However, liver-expressed antimicrobial peptide 2A/B mRNA in the DI and IL-6, IL-17D, IL-10, transforming growth factor (TGF)-beta 1 and TGF-beta 2 mRNA in the PI were not affected by vitamin A levels. Meanwhile, vitamin A deficiency disturbed inflammatory cytokines in the PI, MI and DI, which might be partly linked to p38 mitogen-activated protein kinase (p38MAPK) signalling and NF-kappa B canonical signalling pathway (I kappa B kinase beta (IKK beta), IKK gamma, inhibitor of kappa B alpha, NF-kappa B p65 and c-Rel) rather than NF-kappa B non-canonical signalling pathway (NF-kappa B p52 and IKK alpha). However, the signalling molecules NF-kappa B p65 and p38MAPK did not participate in regulating cytokines in the PI. These results suggested that vitamin A deficiency decreased fish growth and impaired intestinal immune function, and that different immune responses in the PI, MI and DI were mediated partly by NF-kappa B canonical signalling and p38MAPK signalling pathways. On the basis of percentage of weight gain, to protect fish against enteritis morbidity and acid phosphatase activity, the optimum dietary vitamin A levels were estimated to be 0.664, 0.707 and 0.722 mg/kg, respectively. |
format |
Report |
author |
Zhang, Li Feng, Lin Jiang, Wei-Dan Liu, Yang Wu, Pei Kuang, Sheng-Yao Tang, Ling Tang, Wu-Neng Zhang, Yong-An Zhou, Xiao-Qiu |
author_facet |
Zhang, Li Feng, Lin Jiang, Wei-Dan Liu, Yang Wu, Pei Kuang, Sheng-Yao Tang, Ling Tang, Wu-Neng Zhang, Yong-An Zhou, Xiao-Qiu |
author_sort |
Zhang, Li |
title |
Vitamin A deficiency suppresses fish immune function with differences in different intestinal segments: the role of transcriptional factor NF-kappa B and p38 mitogen-activated protein kinase signalling pathways |
title_short |
Vitamin A deficiency suppresses fish immune function with differences in different intestinal segments: the role of transcriptional factor NF-kappa B and p38 mitogen-activated protein kinase signalling pathways |
title_full |
Vitamin A deficiency suppresses fish immune function with differences in different intestinal segments: the role of transcriptional factor NF-kappa B and p38 mitogen-activated protein kinase signalling pathways |
title_fullStr |
Vitamin A deficiency suppresses fish immune function with differences in different intestinal segments: the role of transcriptional factor NF-kappa B and p38 mitogen-activated protein kinase signalling pathways |
title_full_unstemmed |
Vitamin A deficiency suppresses fish immune function with differences in different intestinal segments: the role of transcriptional factor NF-kappa B and p38 mitogen-activated protein kinase signalling pathways |
title_sort |
vitamin a deficiency suppresses fish immune function with differences in different intestinal segments: the role of transcriptional factor nf-kappa b and p38 mitogen-activated protein kinase signalling pathways |
publisher |
CAMBRIDGE UNIV PRESS |
publishDate |
2017 |
url |
http://ir.ihb.ac.cn/handle/342005/32973 http://ir.ihb.ac.cn/handle/342005/32974 https://doi.org/10.1017/S0007114516003342 |
genre |
Atlantic salmon Salmo salar |
genre_facet |
Atlantic salmon Salmo salar |
op_relation |
BRITISH JOURNAL OF NUTRITION http://ir.ihb.ac.cn/handle/342005/32973 http://ir.ihb.ac.cn/handle/342005/32974 doi:10.1017/S0007114516003342 |
op_doi |
https://doi.org/10.1017/S0007114516003342 |
container_title |
British Journal of Nutrition |
container_volume |
117 |
container_issue |
1 |
container_start_page |
67 |
op_container_end_page |
82 |
_version_ |
1766363529859825664 |