Intestinal disease of scattered mirror carp Cyprinus carpio caused by Thelohanellus kitauei and notes on the morphology and phylogeny of the myxosporean from Sichuan Province, southwest China
The mass mortality of pond-reared scattered mirror carp, Cyprinus carpio, caused by Thelohanellus kitauei, occurred at fish farms in Sichuan Province, southwest China. Morphological and molecular analyses were supplemented with histological evaluation of infected tissues to better understand the rou...
Published in: | Chinese Journal of Oceanology and Limnology |
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Language: | English |
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SCIENCE PRESS
2017
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Online Access: | http://ir.ihb.ac.cn/handle/342005/32786 https://doi.org/10.1007/s00343-017-5312-5 |
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ftchinacadsciihb:oai:ir.ihb.ac.cn:342005/32786 2023-05-15T18:15:53+02:00 Intestinal disease of scattered mirror carp Cyprinus carpio caused by Thelohanellus kitauei and notes on the morphology and phylogeny of the myxosporean from Sichuan Province, southwest China Ye Lingtong Lu Mingmiao Quan Keyan Li Wenxiang Zou Hong Wu Shangong Wang Jiangyong Wang Guitang 2017-05-01 http://ir.ihb.ac.cn/handle/342005/32786 https://doi.org/10.1007/s00343-017-5312-5 英语 eng SCIENCE PRESS CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY http://ir.ihb.ac.cn/handle/342005/32786 doi:10.1007/s00343-017-5312-5 intestinal disease scattered mirror carp Thelohanellus kitauei host-specific tropism morphology phylogeny Marine & Freshwater Biology Oceanography Limnology AURATUS-GIBELIO BLOCH TURBOT SCOPHTHALMUS-MAXIMUS N. SP MYXOSPOREA RDNA SEQUENCES MYXOBOLUS-AMPULLICAPSULATUS MYXOZOA PARASITE SEA TRANSMISSION BIVALVULIDA 期刊论文 2017 ftchinacadsciihb https://doi.org/10.1007/s00343-017-5312-5 2019-10-04T00:04:20Z The mass mortality of pond-reared scattered mirror carp, Cyprinus carpio, caused by Thelohanellus kitauei, occurred at fish farms in Sichuan Province, southwest China. Morphological and molecular analyses were supplemented with histological evaluation of infected tissues to better understand the route of infection and the pathological effects of T. kitauei on the fish host. The intestine of the diseased host was full of large cysts of the myxosporean. The cysts range from 2 cm to 3.6 cm in diameter. Histopathology indicated that T. kitauei first invaded the submucosa of the host intestine and then moved into the mucosa layers with the development of their spores, finally entering into the enteric cavity of the hosts after the disruption of mucosa layers. The pyriform spores of T. kitauei were surrounded by the transparent spore sheath, measuring 25.98 mu m +/- 0.95 mu m in body length, 8.72 mu m +/- 0.51 mu m in body width, and 7.86 mu m +/- 0.26 mu m in body thickness. The single polar capsule was pyriform, measuring 14.73 mu m +/- 0.92 mu m in length and 6.82 mu m +/- 0.45 mu m in width, with eight to 10 turns of filament coils winding inside. Phylogenetic analysis based on the 18S small-subunit ribosomal DNA sequences indicated that minimal genetic differences were present between T. kitauei samples from South Korea and from China. Close affinity was found between the genus Thelohanellus and Myxobolus. Additionally, two polar capsule nuclei were found at the anterior end of the single polar capsule in spores of T. kitauei stained with hematoxylin and eosin, which suggested the separation of the genus Thelohanellus from Myxobolus. Report Scophthalmus maximus Turbot Institute of Hydrobiology, Chinese Academy of Sciences: IHB OpenIR Chinese Journal of Oceanology and Limnology 35 3 587 596 |
institution |
Open Polar |
collection |
Institute of Hydrobiology, Chinese Academy of Sciences: IHB OpenIR |
op_collection_id |
ftchinacadsciihb |
language |
English |
topic |
intestinal disease scattered mirror carp Thelohanellus kitauei host-specific tropism morphology phylogeny Marine & Freshwater Biology Oceanography Limnology AURATUS-GIBELIO BLOCH TURBOT SCOPHTHALMUS-MAXIMUS N. SP MYXOSPOREA RDNA SEQUENCES MYXOBOLUS-AMPULLICAPSULATUS MYXOZOA PARASITE SEA TRANSMISSION BIVALVULIDA |
spellingShingle |
intestinal disease scattered mirror carp Thelohanellus kitauei host-specific tropism morphology phylogeny Marine & Freshwater Biology Oceanography Limnology AURATUS-GIBELIO BLOCH TURBOT SCOPHTHALMUS-MAXIMUS N. SP MYXOSPOREA RDNA SEQUENCES MYXOBOLUS-AMPULLICAPSULATUS MYXOZOA PARASITE SEA TRANSMISSION BIVALVULIDA Ye Lingtong Lu Mingmiao Quan Keyan Li Wenxiang Zou Hong Wu Shangong Wang Jiangyong Wang Guitang Intestinal disease of scattered mirror carp Cyprinus carpio caused by Thelohanellus kitauei and notes on the morphology and phylogeny of the myxosporean from Sichuan Province, southwest China |
topic_facet |
intestinal disease scattered mirror carp Thelohanellus kitauei host-specific tropism morphology phylogeny Marine & Freshwater Biology Oceanography Limnology AURATUS-GIBELIO BLOCH TURBOT SCOPHTHALMUS-MAXIMUS N. SP MYXOSPOREA RDNA SEQUENCES MYXOBOLUS-AMPULLICAPSULATUS MYXOZOA PARASITE SEA TRANSMISSION BIVALVULIDA |
description |
The mass mortality of pond-reared scattered mirror carp, Cyprinus carpio, caused by Thelohanellus kitauei, occurred at fish farms in Sichuan Province, southwest China. Morphological and molecular analyses were supplemented with histological evaluation of infected tissues to better understand the route of infection and the pathological effects of T. kitauei on the fish host. The intestine of the diseased host was full of large cysts of the myxosporean. The cysts range from 2 cm to 3.6 cm in diameter. Histopathology indicated that T. kitauei first invaded the submucosa of the host intestine and then moved into the mucosa layers with the development of their spores, finally entering into the enteric cavity of the hosts after the disruption of mucosa layers. The pyriform spores of T. kitauei were surrounded by the transparent spore sheath, measuring 25.98 mu m +/- 0.95 mu m in body length, 8.72 mu m +/- 0.51 mu m in body width, and 7.86 mu m +/- 0.26 mu m in body thickness. The single polar capsule was pyriform, measuring 14.73 mu m +/- 0.92 mu m in length and 6.82 mu m +/- 0.45 mu m in width, with eight to 10 turns of filament coils winding inside. Phylogenetic analysis based on the 18S small-subunit ribosomal DNA sequences indicated that minimal genetic differences were present between T. kitauei samples from South Korea and from China. Close affinity was found between the genus Thelohanellus and Myxobolus. Additionally, two polar capsule nuclei were found at the anterior end of the single polar capsule in spores of T. kitauei stained with hematoxylin and eosin, which suggested the separation of the genus Thelohanellus from Myxobolus. |
format |
Report |
author |
Ye Lingtong Lu Mingmiao Quan Keyan Li Wenxiang Zou Hong Wu Shangong Wang Jiangyong Wang Guitang |
author_facet |
Ye Lingtong Lu Mingmiao Quan Keyan Li Wenxiang Zou Hong Wu Shangong Wang Jiangyong Wang Guitang |
author_sort |
Ye Lingtong |
title |
Intestinal disease of scattered mirror carp Cyprinus carpio caused by Thelohanellus kitauei and notes on the morphology and phylogeny of the myxosporean from Sichuan Province, southwest China |
title_short |
Intestinal disease of scattered mirror carp Cyprinus carpio caused by Thelohanellus kitauei and notes on the morphology and phylogeny of the myxosporean from Sichuan Province, southwest China |
title_full |
Intestinal disease of scattered mirror carp Cyprinus carpio caused by Thelohanellus kitauei and notes on the morphology and phylogeny of the myxosporean from Sichuan Province, southwest China |
title_fullStr |
Intestinal disease of scattered mirror carp Cyprinus carpio caused by Thelohanellus kitauei and notes on the morphology and phylogeny of the myxosporean from Sichuan Province, southwest China |
title_full_unstemmed |
Intestinal disease of scattered mirror carp Cyprinus carpio caused by Thelohanellus kitauei and notes on the morphology and phylogeny of the myxosporean from Sichuan Province, southwest China |
title_sort |
intestinal disease of scattered mirror carp cyprinus carpio caused by thelohanellus kitauei and notes on the morphology and phylogeny of the myxosporean from sichuan province, southwest china |
publisher |
SCIENCE PRESS |
publishDate |
2017 |
url |
http://ir.ihb.ac.cn/handle/342005/32786 https://doi.org/10.1007/s00343-017-5312-5 |
genre |
Scophthalmus maximus Turbot |
genre_facet |
Scophthalmus maximus Turbot |
op_relation |
CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY http://ir.ihb.ac.cn/handle/342005/32786 doi:10.1007/s00343-017-5312-5 |
op_doi |
https://doi.org/10.1007/s00343-017-5312-5 |
container_title |
Chinese Journal of Oceanology and Limnology |
container_volume |
35 |
container_issue |
3 |
container_start_page |
587 |
op_container_end_page |
596 |
_version_ |
1766189139741376512 |