Effects of dietary glycinin on the growth performance, digestion, intestinal morphology and bacterial community of juvenile turbot, Scophthalmus maximus L.
This study was run to investigate effects of dietary glycinin on the growth performance, digestion, intestinal morphology and bacterial community of juvenile turbot (Scophthalmus maximus L.). Graded levels (0%, 3%, 6% and 12%) of purified glycinin were added to the basal diet to formulate four exper...
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Language: | English |
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Online Access: | http://ir.ihb.ac.cn/handle/342005/31527 http://ir.ihb.ac.cn/handle/342005/31528 https://doi.org/10.1016/j.aquaculture.2017.05.008 |
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ftchinacadsciihb:oai:ir.ihb.ac.cn:342005/31528 2023-05-15T16:19:21+02:00 Effects of dietary glycinin on the growth performance, digestion, intestinal morphology and bacterial community of juvenile turbot, Scophthalmus maximus L. Li, Yanxian Yang, Pei Zhang, Yanjiao Ai, Qinghui Xu, Wei Zhang, Wenbing Zhang, Yongan Hu, Haibin Liu, Jintao Mai, Kangsen 2017-10-01 http://ir.ihb.ac.cn/handle/342005/31527 http://ir.ihb.ac.cn/handle/342005/31528 https://doi.org/10.1016/j.aquaculture.2017.05.008 英语 eng ELSEVIER SCIENCE BV AQUACULTURE http://ir.ihb.ac.cn/handle/342005/31527 http://ir.ihb.ac.cn/handle/342005/31528 doi:10.1016/j.aquaculture.2017.05.008 Glycinin Growth performance Digestibility Intestinal morphology Microbiota Turbot Fisheries Marine & Freshwater Biology SOYBEAN BETA-CONGLYCININ GADUS-MORHUA L IN-VITRO RAINBOW-TROUT ONCORHYNCHUS-MYKISS PRERUMINANT CALVES FOOD ALLERGENS MEAL PROTEINS METABOLISM 期刊论文 2017 ftchinacadsciihb https://doi.org/10.1016/j.aquaculture.2017.05.008 2019-08-23T00:03:47Z This study was run to investigate effects of dietary glycinin on the growth performance, digestion, intestinal morphology and bacterial community of juvenile turbot (Scophthalmus maximus L.). Graded levels (0%, 3%, 6% and 12%) of purified glycinin were added to the basal diet to formulate four experimental diets containing 0%, 2.18%, 4.17% and 8.31% immunologically active glycinin, respectively. Triplicate groups of 30 fish were fed to apparent satiation twice daily for 12 weeks. Dietary inclusion of glycinin did not affect feed intake, feed efficiency ratio or weight gain of turbot, however, fish fed 12% glycinin showed a significant decrease in the apparent digestibility of dry matter and protein, as well as the whole-body lipid content. Meanwhile, slightly increased infiltration of mixed leukocytes in the lamina propria was observed in turbot fed 12% glycinin, so was specific IgM against glycinin in the serum. Sequencing of bacterial 16s rRNA V-4 region showed that Proteobacteria (90.64%), Bacteroidetes (6.01%) and Firmicutes (1.73%) were the dominant bacteria in the intestine of turbot. Dietary inclusion of glycinin had no significant effect on the overall structure of bacterial community but dramatically reduced the relative abundance of the Vibrio genus, one of the core microbes, in a dose-independent manner. Our results suggest that turbot can tolerate a relatively high level of immunologically active glycinin (8.31%) in the diet without showing notable impairments of growth performance and gut function. Report Gadus morhua Scophthalmus maximus Turbot Institute of Hydrobiology, Chinese Academy of Sciences: IHB OpenIR Aquaculture 479 125 133 |
institution |
Open Polar |
collection |
Institute of Hydrobiology, Chinese Academy of Sciences: IHB OpenIR |
op_collection_id |
ftchinacadsciihb |
language |
English |
topic |
Glycinin Growth performance Digestibility Intestinal morphology Microbiota Turbot Fisheries Marine & Freshwater Biology SOYBEAN BETA-CONGLYCININ GADUS-MORHUA L IN-VITRO RAINBOW-TROUT ONCORHYNCHUS-MYKISS PRERUMINANT CALVES FOOD ALLERGENS MEAL PROTEINS METABOLISM |
spellingShingle |
Glycinin Growth performance Digestibility Intestinal morphology Microbiota Turbot Fisheries Marine & Freshwater Biology SOYBEAN BETA-CONGLYCININ GADUS-MORHUA L IN-VITRO RAINBOW-TROUT ONCORHYNCHUS-MYKISS PRERUMINANT CALVES FOOD ALLERGENS MEAL PROTEINS METABOLISM Li, Yanxian Yang, Pei Zhang, Yanjiao Ai, Qinghui Xu, Wei Zhang, Wenbing Zhang, Yongan Hu, Haibin Liu, Jintao Mai, Kangsen Effects of dietary glycinin on the growth performance, digestion, intestinal morphology and bacterial community of juvenile turbot, Scophthalmus maximus L. |
topic_facet |
Glycinin Growth performance Digestibility Intestinal morphology Microbiota Turbot Fisheries Marine & Freshwater Biology SOYBEAN BETA-CONGLYCININ GADUS-MORHUA L IN-VITRO RAINBOW-TROUT ONCORHYNCHUS-MYKISS PRERUMINANT CALVES FOOD ALLERGENS MEAL PROTEINS METABOLISM |
description |
This study was run to investigate effects of dietary glycinin on the growth performance, digestion, intestinal morphology and bacterial community of juvenile turbot (Scophthalmus maximus L.). Graded levels (0%, 3%, 6% and 12%) of purified glycinin were added to the basal diet to formulate four experimental diets containing 0%, 2.18%, 4.17% and 8.31% immunologically active glycinin, respectively. Triplicate groups of 30 fish were fed to apparent satiation twice daily for 12 weeks. Dietary inclusion of glycinin did not affect feed intake, feed efficiency ratio or weight gain of turbot, however, fish fed 12% glycinin showed a significant decrease in the apparent digestibility of dry matter and protein, as well as the whole-body lipid content. Meanwhile, slightly increased infiltration of mixed leukocytes in the lamina propria was observed in turbot fed 12% glycinin, so was specific IgM against glycinin in the serum. Sequencing of bacterial 16s rRNA V-4 region showed that Proteobacteria (90.64%), Bacteroidetes (6.01%) and Firmicutes (1.73%) were the dominant bacteria in the intestine of turbot. Dietary inclusion of glycinin had no significant effect on the overall structure of bacterial community but dramatically reduced the relative abundance of the Vibrio genus, one of the core microbes, in a dose-independent manner. Our results suggest that turbot can tolerate a relatively high level of immunologically active glycinin (8.31%) in the diet without showing notable impairments of growth performance and gut function. |
format |
Report |
author |
Li, Yanxian Yang, Pei Zhang, Yanjiao Ai, Qinghui Xu, Wei Zhang, Wenbing Zhang, Yongan Hu, Haibin Liu, Jintao Mai, Kangsen |
author_facet |
Li, Yanxian Yang, Pei Zhang, Yanjiao Ai, Qinghui Xu, Wei Zhang, Wenbing Zhang, Yongan Hu, Haibin Liu, Jintao Mai, Kangsen |
author_sort |
Li, Yanxian |
title |
Effects of dietary glycinin on the growth performance, digestion, intestinal morphology and bacterial community of juvenile turbot, Scophthalmus maximus L. |
title_short |
Effects of dietary glycinin on the growth performance, digestion, intestinal morphology and bacterial community of juvenile turbot, Scophthalmus maximus L. |
title_full |
Effects of dietary glycinin on the growth performance, digestion, intestinal morphology and bacterial community of juvenile turbot, Scophthalmus maximus L. |
title_fullStr |
Effects of dietary glycinin on the growth performance, digestion, intestinal morphology and bacterial community of juvenile turbot, Scophthalmus maximus L. |
title_full_unstemmed |
Effects of dietary glycinin on the growth performance, digestion, intestinal morphology and bacterial community of juvenile turbot, Scophthalmus maximus L. |
title_sort |
effects of dietary glycinin on the growth performance, digestion, intestinal morphology and bacterial community of juvenile turbot, scophthalmus maximus l. |
publisher |
ELSEVIER SCIENCE BV |
publishDate |
2017 |
url |
http://ir.ihb.ac.cn/handle/342005/31527 http://ir.ihb.ac.cn/handle/342005/31528 https://doi.org/10.1016/j.aquaculture.2017.05.008 |
genre |
Gadus morhua Scophthalmus maximus Turbot |
genre_facet |
Gadus morhua Scophthalmus maximus Turbot |
op_relation |
AQUACULTURE http://ir.ihb.ac.cn/handle/342005/31527 http://ir.ihb.ac.cn/handle/342005/31528 doi:10.1016/j.aquaculture.2017.05.008 |
op_doi |
https://doi.org/10.1016/j.aquaculture.2017.05.008 |
container_title |
Aquaculture |
container_volume |
479 |
container_start_page |
125 |
op_container_end_page |
133 |
_version_ |
1766005727670829056 |