Threonine deficiency decreased intestinal immunity and aggravated inflammation associated with NF-kappa B and i signalling pathways in juvenile grass carp (Ctenopharyngodon idella) after infection with Aeromonas hydrophila

This study aimed to investigate the impacts of dietary threonine on intestinal immunity and inflammation in juvenile grass carp. Six isonitrogenous semi-purified diets containing graded levels of threonine (3.99-21.66 g threonine/kg) were formulated and fed to fishes for 8 weeks, and then challenged...

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Bibliographic Details
Published in:British Journal of Nutrition
Main Authors: Dong, Yu-Wen, Jiang, Wei-Dan, Liu, Yang, Wu, Pei, Jiang, Jun, Kuang, Sheng-Yao, Tang, Ling, Tang, Wu-Neng, Zhang, Yong-An, Zhou, Xiao-Qiu, Feng, Lin
Format: Report
Language:English
Published: CAMBRIDGE UNIV PRESS 2017
Subjects:
Threonine deficiency
Intestine
Immunity
Inflammation
NF-kappa B and target of rapamycin signalling
Juvenile grass carp (Ctenopharyngodon idella)
Nutrition & Dietetics
BREAM MEGALOBRAMA-AMBLYCEPHALA
BASS DICENTRARCHUS-LABRAX
TIGHT JUNCTION PROTEINS
SALMON SALMO-SALAR
GENE-EXPRESSION
ANTIOXIDANT STATUS
DIETARY THREONINE
GROWTH-PERFORMANCE
BARRIER FUNCTION
NONSPECIFIC IMMUNITY
Salmo salar
Online Access:http://ir.ihb.ac.cn/handle/342005/31515
http://ir.ihb.ac.cn/handle/342005/31516
https://doi.org/10.1017/S0007114517001830
Description
Summary:This study aimed to investigate the impacts of dietary threonine on intestinal immunity and inflammation in juvenile grass carp. Six isonitrogenous semi-purified diets containing graded levels of threonine (3.99-21.66 g threonine/kg) were formulated and fed to fishes for 8 weeks, and then challenged with Aeromonas hydrophila for 14 d. Results showed that, compared with optimum threonine supplementation, threonine deficiency (1) decreased the ability of fish against enteritis, intestinal lysozyme activities (except in the distal intestine), acid phosphatase activities, complement 3 (C3) and C4 contents and IgM contents (except in the proximal intestine (PI)), and it down-regulated the transcript abundances of liver-expressed antimicrobial peptide (LEAP)-2A, LEAP-2B, hepcidin, IgZ, IgM and beta-defensin1 (except in the PI) (P < 0.05); (2) could up-regulate intestinal pro-inflammatory cytokines TNF-alpha, IL-1 beta, IL-6, IL-8 and IL-17D mRNA levels partly related to NF-kappa B signalling; (3) could down-regulate intestinal anti-inflammatory cytokine transforming growth factor (TGF)-beta(1), TGF-beta(2), IL-4/13A (not IL-4/13B) and IL-10 mRNA levels partly by target of rapamycin signalling. Finally, on the basis of the specific growth rate, against the enteritis morbidity and IgM contents, the optimum threonine requirements were estimated to be 14.53 g threonine/kg diet (4.48 g threonine/100 g protein), 15.05 g threonine/kg diet (4.64 g threonine/100 g protein) and 15.17 g threonine/kg diet (4.68 g threonine/100 g protein), respectively.

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