Threonine deficiency decreased intestinal immunity and aggravated inflammation associated with NF-kappa B and i signalling pathways in juvenile grass carp (Ctenopharyngodon idella) after infection with Aeromonas hydrophila
This study aimed to investigate the impacts of dietary threonine on intestinal immunity and inflammation in juvenile grass carp. Six isonitrogenous semi-purified diets containing graded levels of threonine (3.99-21.66 g threonine/kg) were formulated and fed to fishes for 8 weeks, and then challenged...
Published in: | British Journal of Nutrition |
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Language: | English |
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CAMBRIDGE UNIV PRESS
2017
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Online Access: | http://ir.ihb.ac.cn/handle/342005/31514 https://doi.org/10.1017/S0007114517001830 |
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ftchinacadsciihb:oai:ir.ihb.ac.cn:342005/31514 2023-05-15T18:10:01+02:00 Threonine deficiency decreased intestinal immunity and aggravated inflammation associated with NF-kappa B and i signalling pathways in juvenile grass carp (Ctenopharyngodon idella) after infection with Aeromonas hydrophila Dong, Yu-Wen Jiang, Wei-Dan Liu, Yang Wu, Pei Jiang, Jun Kuang, Sheng-Yao Tang, Ling Tang, Wu-Neng Zhang, Yong-An Zhou, Xiao-Qiu Feng, Lin 2017-07-01 http://ir.ihb.ac.cn/handle/342005/31514 https://doi.org/10.1017/S0007114517001830 英语 eng CAMBRIDGE UNIV PRESS BRITISH JOURNAL OF NUTRITION http://ir.ihb.ac.cn/handle/342005/31514 doi:10.1017/S0007114517001830 Threonine deficiency Intestine Immunity Inflammation NF-kappa B and target of rapamycin signalling Juvenile grass carp (Ctenopharyngodon idella) Nutrition & Dietetics BREAM MEGALOBRAMA-AMBLYCEPHALA BASS DICENTRARCHUS-LABRAX TIGHT JUNCTION PROTEINS SALMON SALMO-SALAR GENE-EXPRESSION ANTIOXIDANT STATUS DIETARY THREONINE GROWTH-PERFORMANCE BARRIER FUNCTION NONSPECIFIC IMMUNITY 期刊论文 2017 ftchinacadsciihb https://doi.org/10.1017/S0007114517001830 2019-08-02T00:03:31Z This study aimed to investigate the impacts of dietary threonine on intestinal immunity and inflammation in juvenile grass carp. Six isonitrogenous semi-purified diets containing graded levels of threonine (3.99-21.66 g threonine/kg) were formulated and fed to fishes for 8 weeks, and then challenged with Aeromonas hydrophila for 14 d. Results showed that, compared with optimum threonine supplementation, threonine deficiency (1) decreased the ability of fish against enteritis, intestinal lysozyme activities (except in the distal intestine), acid phosphatase activities, complement 3 (C3) and C4 contents and IgM contents (except in the proximal intestine (PI)), and it down-regulated the transcript abundances of liver-expressed antimicrobial peptide (LEAP)-2A, LEAP-2B, hepcidin, IgZ, IgM and beta-defensin1 (except in the PI) (P < 0.05); (2) could up-regulate intestinal pro-inflammatory cytokines TNF-alpha, IL-1 beta, IL-6, IL-8 and IL-17D mRNA levels partly related to NF-kappa B signalling; (3) could down-regulate intestinal anti-inflammatory cytokine transforming growth factor (TGF)-beta(1), TGF-beta(2), IL-4/13A (not IL-4/13B) and IL-10 mRNA levels partly by target of rapamycin signalling. Finally, on the basis of the specific growth rate, against the enteritis morbidity and IgM contents, the optimum threonine requirements were estimated to be 14.53 g threonine/kg diet (4.48 g threonine/100 g protein), 15.05 g threonine/kg diet (4.64 g threonine/100 g protein) and 15.17 g threonine/kg diet (4.68 g threonine/100 g protein), respectively. Report Salmo salar Institute of Hydrobiology, Chinese Academy of Sciences: IHB OpenIR British Journal of Nutrition 118 2 92 108 |
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Open Polar |
collection |
Institute of Hydrobiology, Chinese Academy of Sciences: IHB OpenIR |
op_collection_id |
ftchinacadsciihb |
language |
English |
topic |
Threonine deficiency Intestine Immunity Inflammation NF-kappa B and target of rapamycin signalling Juvenile grass carp (Ctenopharyngodon idella) Nutrition & Dietetics BREAM MEGALOBRAMA-AMBLYCEPHALA BASS DICENTRARCHUS-LABRAX TIGHT JUNCTION PROTEINS SALMON SALMO-SALAR GENE-EXPRESSION ANTIOXIDANT STATUS DIETARY THREONINE GROWTH-PERFORMANCE BARRIER FUNCTION NONSPECIFIC IMMUNITY |
spellingShingle |
Threonine deficiency Intestine Immunity Inflammation NF-kappa B and target of rapamycin signalling Juvenile grass carp (Ctenopharyngodon idella) Nutrition & Dietetics BREAM MEGALOBRAMA-AMBLYCEPHALA BASS DICENTRARCHUS-LABRAX TIGHT JUNCTION PROTEINS SALMON SALMO-SALAR GENE-EXPRESSION ANTIOXIDANT STATUS DIETARY THREONINE GROWTH-PERFORMANCE BARRIER FUNCTION NONSPECIFIC IMMUNITY Dong, Yu-Wen Jiang, Wei-Dan Liu, Yang Wu, Pei Jiang, Jun Kuang, Sheng-Yao Tang, Ling Tang, Wu-Neng Zhang, Yong-An Zhou, Xiao-Qiu Feng, Lin Threonine deficiency decreased intestinal immunity and aggravated inflammation associated with NF-kappa B and i signalling pathways in juvenile grass carp (Ctenopharyngodon idella) after infection with Aeromonas hydrophila |
topic_facet |
Threonine deficiency Intestine Immunity Inflammation NF-kappa B and target of rapamycin signalling Juvenile grass carp (Ctenopharyngodon idella) Nutrition & Dietetics BREAM MEGALOBRAMA-AMBLYCEPHALA BASS DICENTRARCHUS-LABRAX TIGHT JUNCTION PROTEINS SALMON SALMO-SALAR GENE-EXPRESSION ANTIOXIDANT STATUS DIETARY THREONINE GROWTH-PERFORMANCE BARRIER FUNCTION NONSPECIFIC IMMUNITY |
description |
This study aimed to investigate the impacts of dietary threonine on intestinal immunity and inflammation in juvenile grass carp. Six isonitrogenous semi-purified diets containing graded levels of threonine (3.99-21.66 g threonine/kg) were formulated and fed to fishes for 8 weeks, and then challenged with Aeromonas hydrophila for 14 d. Results showed that, compared with optimum threonine supplementation, threonine deficiency (1) decreased the ability of fish against enteritis, intestinal lysozyme activities (except in the distal intestine), acid phosphatase activities, complement 3 (C3) and C4 contents and IgM contents (except in the proximal intestine (PI)), and it down-regulated the transcript abundances of liver-expressed antimicrobial peptide (LEAP)-2A, LEAP-2B, hepcidin, IgZ, IgM and beta-defensin1 (except in the PI) (P < 0.05); (2) could up-regulate intestinal pro-inflammatory cytokines TNF-alpha, IL-1 beta, IL-6, IL-8 and IL-17D mRNA levels partly related to NF-kappa B signalling; (3) could down-regulate intestinal anti-inflammatory cytokine transforming growth factor (TGF)-beta(1), TGF-beta(2), IL-4/13A (not IL-4/13B) and IL-10 mRNA levels partly by target of rapamycin signalling. Finally, on the basis of the specific growth rate, against the enteritis morbidity and IgM contents, the optimum threonine requirements were estimated to be 14.53 g threonine/kg diet (4.48 g threonine/100 g protein), 15.05 g threonine/kg diet (4.64 g threonine/100 g protein) and 15.17 g threonine/kg diet (4.68 g threonine/100 g protein), respectively. |
format |
Report |
author |
Dong, Yu-Wen Jiang, Wei-Dan Liu, Yang Wu, Pei Jiang, Jun Kuang, Sheng-Yao Tang, Ling Tang, Wu-Neng Zhang, Yong-An Zhou, Xiao-Qiu Feng, Lin |
author_facet |
Dong, Yu-Wen Jiang, Wei-Dan Liu, Yang Wu, Pei Jiang, Jun Kuang, Sheng-Yao Tang, Ling Tang, Wu-Neng Zhang, Yong-An Zhou, Xiao-Qiu Feng, Lin |
author_sort |
Dong, Yu-Wen |
title |
Threonine deficiency decreased intestinal immunity and aggravated inflammation associated with NF-kappa B and i signalling pathways in juvenile grass carp (Ctenopharyngodon idella) after infection with Aeromonas hydrophila |
title_short |
Threonine deficiency decreased intestinal immunity and aggravated inflammation associated with NF-kappa B and i signalling pathways in juvenile grass carp (Ctenopharyngodon idella) after infection with Aeromonas hydrophila |
title_full |
Threonine deficiency decreased intestinal immunity and aggravated inflammation associated with NF-kappa B and i signalling pathways in juvenile grass carp (Ctenopharyngodon idella) after infection with Aeromonas hydrophila |
title_fullStr |
Threonine deficiency decreased intestinal immunity and aggravated inflammation associated with NF-kappa B and i signalling pathways in juvenile grass carp (Ctenopharyngodon idella) after infection with Aeromonas hydrophila |
title_full_unstemmed |
Threonine deficiency decreased intestinal immunity and aggravated inflammation associated with NF-kappa B and i signalling pathways in juvenile grass carp (Ctenopharyngodon idella) after infection with Aeromonas hydrophila |
title_sort |
threonine deficiency decreased intestinal immunity and aggravated inflammation associated with nf-kappa b and i signalling pathways in juvenile grass carp (ctenopharyngodon idella) after infection with aeromonas hydrophila |
publisher |
CAMBRIDGE UNIV PRESS |
publishDate |
2017 |
url |
http://ir.ihb.ac.cn/handle/342005/31514 https://doi.org/10.1017/S0007114517001830 |
genre |
Salmo salar |
genre_facet |
Salmo salar |
op_relation |
BRITISH JOURNAL OF NUTRITION http://ir.ihb.ac.cn/handle/342005/31514 doi:10.1017/S0007114517001830 |
op_doi |
https://doi.org/10.1017/S0007114517001830 |
container_title |
British Journal of Nutrition |
container_volume |
118 |
container_issue |
2 |
container_start_page |
92 |
op_container_end_page |
108 |
_version_ |
1766182731864080384 |