Decreased enteritis resistance ability by dietary low or excess levels of lipids through impairing the intestinal physical and immune barriers function of young grass carp (Ctenopharyngodon idella)
The current study explores the hypothesis that low or excess levels of lipids decrease the enteritis resistance ability through impairing the intestinal physical and immune barrier function of young grass carp (Ctenopharyngodon idella). A total of 540 young grass carp with an average initial weight...
Published in: | Fish & Shellfish Immunology |
---|---|
Main Authors: | , , , , , , , , , , , , |
Format: | Report |
Language: | English |
Published: |
ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD
2017
|
Subjects: | |
Online Access: | http://ir.ihb.ac.cn/handle/342005/31418 https://doi.org/10.1016/j.fsi.2017.06.041 |
id |
ftchinacadsciihb:oai:ir.ihb.ac.cn:342005/31418 |
---|---|
record_format |
openpolar |
spelling |
ftchinacadsciihb:oai:ir.ihb.ac.cn:342005/31418 2023-05-15T15:33:07+02:00 Decreased enteritis resistance ability by dietary low or excess levels of lipids through impairing the intestinal physical and immune barriers function of young grass carp (Ctenopharyngodon idella) Feng, Lin Ni, Pei-Jun Jiang, Wei-Dan Wu, Pei Liu, Yang Jiang, Jun Kuang, Sheng-Yao Tang, Ling Tang, Wu-Neng Zhang, Yong-An Tang, Xu Shi, He-Qun Zhou, Xiao-Qiu 2017-08-01 http://ir.ihb.ac.cn/handle/342005/31418 https://doi.org/10.1016/j.fsi.2017.06.041 英语 eng ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD FISH & SHELLFISH IMMUNOLOGY http://ir.ihb.ac.cn/handle/342005/31418 doi:10.1016/j.fsi.2017.06.041 Ctenopharyngodon idella Lipids Intestine Bacterial challenge Physical barrier Immune barrier Fisheries Immunology Marine & Freshwater Biology Veterinary Sciences NF-KAPPA-B MESSENGER-RNA EXPRESSION TIGHT JUNCTION PROTEINS SALMON SALMO-SALAR PROLIFERATOR-ACTIVATED RECEPTORS SALVELINUS-ALPINUS L AEROMONAS-HYDROPHILA DISEASE RESISTANCE CYPRINUS-CARPIO ATLANTIC SALMON 期刊论文 2017 ftchinacadsciihb https://doi.org/10.1016/j.fsi.2017.06.041 2019-08-02T00:03:28Z The current study explores the hypothesis that low or excess levels of lipids decrease the enteritis resistance ability through impairing the intestinal physical and immune barrier function of young grass carp (Ctenopharyngodon idella). A total of 540 young grass carp with an average initial weight of 261.41 +/- 0.53 g were fed diets containing six graded levels of lipids at 5.9, 21.4, 36.0, 50.2, 66.6 and 80.1 g/kg for 8 weeks. After that, a challenge trial was conducted by injection of Aeromonas hydrophila over 2 weeks. The results show that compared with optimal lipids level, low or excess levels of lipids impair fish immune barrier function through declining humoral compounds and down-regulating the mRNA levels of interleukin 4/13A (IL-4(13)A) [not in the proximal intestine (P0], IL-4(13)B, IL-6, IL-10, transforming growth factor beta 1 (TGF-beta 1), inhibitor of KB alpha (I kappa B alpha), TOR and ribosomal p70S6 kinase (S6K1) (P < 0.05), and up-regulating tumor necrosis factor alpha (TNF-alpha), interferon gamma 2 (IFN-gamma 2), IL-1 beta, IL-8, IL-12 p40 (not p35), nuclear factor kappa B p65 (NF-kappa B p65), I kappa B kinase alpha (IKK alpha), IKK beta, IKK gamma, and elF4E-binding protein (4EBP) in the intestine of young grass carp (P < 0.05). In addition, low or excess levels of lipids also decrease young grass carp physical barrier function through down-regulating the mRNA levels of zonula occludens (ZO-1), ZO-2b [only in the distal intestine (DI)], Claudin b, c, 3, 12, 15a, 15b (only in the DI), 7b (not 7a) and Occludin by MAPKK 6/p38 MAPK (not JNK)/MLCK signaling molecules, down-regulating B-cell lymphoma-2 (Bcl-2) and inhibitor of apoptosis protein (IAP) and up-regulating the mRNA levels of apoptotic protease activating factor-1 (Apaf-1), Caspase-3, -8 and -9 and Fas ligand (FasL) (not in the DI), and decreasing antioxidant ability via Kelch-like ECH-associating protein 1 (Keap1)/NF-E2-related factor 2 (Nrf2) signaling molecules in the intestine. Based on the quadratic regression analysis for the enteritis resistance ability, LA activities and GSH content in the MI were established to be 54.5, 49.91 and 47.83 g lipid/kg diets, respectively. (C) 2017 Elsevier Ltd. All rights reserved. Report Atlantic salmon Salmo salar Salvelinus alpinus Institute of Hydrobiology, Chinese Academy of Sciences: IHB OpenIR Fish & Shellfish Immunology 67 493 512 |
institution |
Open Polar |
collection |
Institute of Hydrobiology, Chinese Academy of Sciences: IHB OpenIR |
op_collection_id |
ftchinacadsciihb |
language |
English |
topic |
Ctenopharyngodon idella Lipids Intestine Bacterial challenge Physical barrier Immune barrier Fisheries Immunology Marine & Freshwater Biology Veterinary Sciences NF-KAPPA-B MESSENGER-RNA EXPRESSION TIGHT JUNCTION PROTEINS SALMON SALMO-SALAR PROLIFERATOR-ACTIVATED RECEPTORS SALVELINUS-ALPINUS L AEROMONAS-HYDROPHILA DISEASE RESISTANCE CYPRINUS-CARPIO ATLANTIC SALMON |
spellingShingle |
Ctenopharyngodon idella Lipids Intestine Bacterial challenge Physical barrier Immune barrier Fisheries Immunology Marine & Freshwater Biology Veterinary Sciences NF-KAPPA-B MESSENGER-RNA EXPRESSION TIGHT JUNCTION PROTEINS SALMON SALMO-SALAR PROLIFERATOR-ACTIVATED RECEPTORS SALVELINUS-ALPINUS L AEROMONAS-HYDROPHILA DISEASE RESISTANCE CYPRINUS-CARPIO ATLANTIC SALMON Feng, Lin Ni, Pei-Jun Jiang, Wei-Dan Wu, Pei Liu, Yang Jiang, Jun Kuang, Sheng-Yao Tang, Ling Tang, Wu-Neng Zhang, Yong-An Tang, Xu Shi, He-Qun Zhou, Xiao-Qiu Decreased enteritis resistance ability by dietary low or excess levels of lipids through impairing the intestinal physical and immune barriers function of young grass carp (Ctenopharyngodon idella) |
topic_facet |
Ctenopharyngodon idella Lipids Intestine Bacterial challenge Physical barrier Immune barrier Fisheries Immunology Marine & Freshwater Biology Veterinary Sciences NF-KAPPA-B MESSENGER-RNA EXPRESSION TIGHT JUNCTION PROTEINS SALMON SALMO-SALAR PROLIFERATOR-ACTIVATED RECEPTORS SALVELINUS-ALPINUS L AEROMONAS-HYDROPHILA DISEASE RESISTANCE CYPRINUS-CARPIO ATLANTIC SALMON |
description |
The current study explores the hypothesis that low or excess levels of lipids decrease the enteritis resistance ability through impairing the intestinal physical and immune barrier function of young grass carp (Ctenopharyngodon idella). A total of 540 young grass carp with an average initial weight of 261.41 +/- 0.53 g were fed diets containing six graded levels of lipids at 5.9, 21.4, 36.0, 50.2, 66.6 and 80.1 g/kg for 8 weeks. After that, a challenge trial was conducted by injection of Aeromonas hydrophila over 2 weeks. The results show that compared with optimal lipids level, low or excess levels of lipids impair fish immune barrier function through declining humoral compounds and down-regulating the mRNA levels of interleukin 4/13A (IL-4(13)A) [not in the proximal intestine (P0], IL-4(13)B, IL-6, IL-10, transforming growth factor beta 1 (TGF-beta 1), inhibitor of KB alpha (I kappa B alpha), TOR and ribosomal p70S6 kinase (S6K1) (P < 0.05), and up-regulating tumor necrosis factor alpha (TNF-alpha), interferon gamma 2 (IFN-gamma 2), IL-1 beta, IL-8, IL-12 p40 (not p35), nuclear factor kappa B p65 (NF-kappa B p65), I kappa B kinase alpha (IKK alpha), IKK beta, IKK gamma, and elF4E-binding protein (4EBP) in the intestine of young grass carp (P < 0.05). In addition, low or excess levels of lipids also decrease young grass carp physical barrier function through down-regulating the mRNA levels of zonula occludens (ZO-1), ZO-2b [only in the distal intestine (DI)], Claudin b, c, 3, 12, 15a, 15b (only in the DI), 7b (not 7a) and Occludin by MAPKK 6/p38 MAPK (not JNK)/MLCK signaling molecules, down-regulating B-cell lymphoma-2 (Bcl-2) and inhibitor of apoptosis protein (IAP) and up-regulating the mRNA levels of apoptotic protease activating factor-1 (Apaf-1), Caspase-3, -8 and -9 and Fas ligand (FasL) (not in the DI), and decreasing antioxidant ability via Kelch-like ECH-associating protein 1 (Keap1)/NF-E2-related factor 2 (Nrf2) signaling molecules in the intestine. Based on the quadratic regression analysis for the enteritis resistance ability, LA activities and GSH content in the MI were established to be 54.5, 49.91 and 47.83 g lipid/kg diets, respectively. (C) 2017 Elsevier Ltd. All rights reserved. |
format |
Report |
author |
Feng, Lin Ni, Pei-Jun Jiang, Wei-Dan Wu, Pei Liu, Yang Jiang, Jun Kuang, Sheng-Yao Tang, Ling Tang, Wu-Neng Zhang, Yong-An Tang, Xu Shi, He-Qun Zhou, Xiao-Qiu |
author_facet |
Feng, Lin Ni, Pei-Jun Jiang, Wei-Dan Wu, Pei Liu, Yang Jiang, Jun Kuang, Sheng-Yao Tang, Ling Tang, Wu-Neng Zhang, Yong-An Tang, Xu Shi, He-Qun Zhou, Xiao-Qiu |
author_sort |
Feng, Lin |
title |
Decreased enteritis resistance ability by dietary low or excess levels of lipids through impairing the intestinal physical and immune barriers function of young grass carp (Ctenopharyngodon idella) |
title_short |
Decreased enteritis resistance ability by dietary low or excess levels of lipids through impairing the intestinal physical and immune barriers function of young grass carp (Ctenopharyngodon idella) |
title_full |
Decreased enteritis resistance ability by dietary low or excess levels of lipids through impairing the intestinal physical and immune barriers function of young grass carp (Ctenopharyngodon idella) |
title_fullStr |
Decreased enteritis resistance ability by dietary low or excess levels of lipids through impairing the intestinal physical and immune barriers function of young grass carp (Ctenopharyngodon idella) |
title_full_unstemmed |
Decreased enteritis resistance ability by dietary low or excess levels of lipids through impairing the intestinal physical and immune barriers function of young grass carp (Ctenopharyngodon idella) |
title_sort |
decreased enteritis resistance ability by dietary low or excess levels of lipids through impairing the intestinal physical and immune barriers function of young grass carp (ctenopharyngodon idella) |
publisher |
ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD |
publishDate |
2017 |
url |
http://ir.ihb.ac.cn/handle/342005/31418 https://doi.org/10.1016/j.fsi.2017.06.041 |
genre |
Atlantic salmon Salmo salar Salvelinus alpinus |
genre_facet |
Atlantic salmon Salmo salar Salvelinus alpinus |
op_relation |
FISH & SHELLFISH IMMUNOLOGY http://ir.ihb.ac.cn/handle/342005/31418 doi:10.1016/j.fsi.2017.06.041 |
op_doi |
https://doi.org/10.1016/j.fsi.2017.06.041 |
container_title |
Fish & Shellfish Immunology |
container_volume |
67 |
container_start_page |
493 |
op_container_end_page |
512 |
_version_ |
1766363596687671296 |