Decreased enteritis resistance ability by dietary low or excess levels of lipids through impairing the intestinal physical and immune barriers function of young grass carp (Ctenopharyngodon idella)

The current study explores the hypothesis that low or excess levels of lipids decrease the enteritis resistance ability through impairing the intestinal physical and immune barrier function of young grass carp (Ctenopharyngodon idella). A total of 540 young grass carp with an average initial weight...

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Bibliographic Details
Published in:Fish & Shellfish Immunology
Main Authors: Feng, Lin, Ni, Pei-Jun, Jiang, Wei-Dan, Wu, Pei, Liu, Yang, Jiang, Jun, Kuang, Sheng-Yao, Tang, Ling, Tang, Wu-Neng, Zhang, Yong-An, Tang, Xu, Shi, He-Qun, Zhou, Xiao-Qiu
Format: Report
Language:English
Published: ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD 2017
Subjects:
Ctenopharyngodon idella
Lipids
Intestine
Bacterial challenge
Physical barrier
Immune barrier
Fisheries
Immunology
Marine & Freshwater Biology
Veterinary Sciences
NF-KAPPA-B
MESSENGER-RNA EXPRESSION
TIGHT JUNCTION PROTEINS
SALMON SALMO-SALAR
PROLIFERATOR-ACTIVATED RECEPTORS
SALVELINUS-ALPINUS L
AEROMONAS-HYDROPHILA
DISEASE RESISTANCE
CYPRINUS-CARPIO
ATLANTIC SALMON
Atlantic salmon
Salmo salar
Salvelinus alpinus
Online Access:http://ir.ihb.ac.cn/handle/342005/31418
https://doi.org/10.1016/j.fsi.2017.06.041
Description
Summary:The current study explores the hypothesis that low or excess levels of lipids decrease the enteritis resistance ability through impairing the intestinal physical and immune barrier function of young grass carp (Ctenopharyngodon idella). A total of 540 young grass carp with an average initial weight of 261.41 +/- 0.53 g were fed diets containing six graded levels of lipids at 5.9, 21.4, 36.0, 50.2, 66.6 and 80.1 g/kg for 8 weeks. After that, a challenge trial was conducted by injection of Aeromonas hydrophila over 2 weeks. The results show that compared with optimal lipids level, low or excess levels of lipids impair fish immune barrier function through declining humoral compounds and down-regulating the mRNA levels of interleukin 4/13A (IL-4(13)A) [not in the proximal intestine (P0], IL-4(13)B, IL-6, IL-10, transforming growth factor beta 1 (TGF-beta 1), inhibitor of KB alpha (I kappa B alpha), TOR and ribosomal p70S6 kinase (S6K1) (P < 0.05), and up-regulating tumor necrosis factor alpha (TNF-alpha), interferon gamma 2 (IFN-gamma 2), IL-1 beta, IL-8, IL-12 p40 (not p35), nuclear factor kappa B p65 (NF-kappa B p65), I kappa B kinase alpha (IKK alpha), IKK beta, IKK gamma, and elF4E-binding protein (4EBP) in the intestine of young grass carp (P < 0.05). In addition, low or excess levels of lipids also decrease young grass carp physical barrier function through down-regulating the mRNA levels of zonula occludens (ZO-1), ZO-2b [only in the distal intestine (DI)], Claudin b, c, 3, 12, 15a, 15b (only in the DI), 7b (not 7a) and Occludin by MAPKK 6/p38 MAPK (not JNK)/MLCK signaling molecules, down-regulating B-cell lymphoma-2 (Bcl-2) and inhibitor of apoptosis protein (IAP) and up-regulating the mRNA levels of apoptotic protease activating factor-1 (Apaf-1), Caspase-3, -8 and -9 and Fas ligand (FasL) (not in the DI), and decreasing antioxidant ability via Kelch-like ECH-associating protein 1 (Keap1)/NF-E2-related factor 2 (Nrf2) signaling molecules in the intestine. Based on the quadratic regression analysis for the enteritis resistance ability, LA activities and GSH content in the MI were established to be 54.5, 49.91 and 47.83 g lipid/kg diets, respectively. (C) 2017 Elsevier Ltd. All rights reserved.

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