The relationship between dietary methionine and growth, digestion, absorption, and antioxidant status in intestinal and hepatopancreatic tissues of sub-adult grass carp (Ctenopharyngodon idella)
Background: Methionine is an essential amino acid for fish. The present study was conducted to investigate the effects of dietary methionine on growth performance, digestive and absorptive ability, as well as antioxidant capacity in the intestine and hepatopancreas of sub-adult grass carp (Ctenophar...
Published in: | Journal of Animal Science and Biotechnology |
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Language: | English |
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BIOMED CENTRAL LTD
2017
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Online Access: | http://ir.ihb.ac.cn/handle/342005/31416 http://ir.ihb.ac.cn/handle/342005/31417 https://doi.org/10.1186/s40104-017-0194-0 |
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ftchinacadsciihb:oai:ir.ihb.ac.cn:342005/31417 2023-05-15T18:10:03+02:00 The relationship between dietary methionine and growth, digestion, absorption, and antioxidant status in intestinal and hepatopancreatic tissues of sub-adult grass carp (Ctenopharyngodon idella) Wu, Pei Tang, Ling Jiang, Weidan Hu, Kai Liu, Yang Jiang, Jun Kuang, Shengyao Tang, Wuneng Zhang, Yongan Zhou, Xiaoqiu Feng, Lin 2017-08-01 http://ir.ihb.ac.cn/handle/342005/31416 http://ir.ihb.ac.cn/handle/342005/31417 https://doi.org/10.1186/s40104-017-0194-0 英语 eng BIOMED CENTRAL LTD JOURNAL OF ANIMAL SCIENCE AND BIOTECHNOLOGY http://ir.ihb.ac.cn/handle/342005/31416 http://ir.ihb.ac.cn/handle/342005/31417 doi:10.1186/s40104-017-0194-0 Antioxidant status Ctenopharyngodon idella Digestive and absorptive capacities Methionine Agriculture Dairy & Animal Science TROUT ONCORHYNCHUS-MYKISS SULFUR AMINO-ACIDS SALMON SALMO-SALAR PREVENTS OXIDATIVE DAMAGE BASS DICENTRARCHUS-LABRAX LARGE YELLOW CROAKER SEMI-PURIFIED DIETS JUVENILE JIAN VAR. JIAN ENZYME-ACTIVITIES 期刊论文 2017 ftchinacadsciihb https://doi.org/10.1186/s40104-017-0194-0 2019-08-23T00:03:47Z Background: Methionine is an essential amino acid for fish. The present study was conducted to investigate the effects of dietary methionine on growth performance, digestive and absorptive ability, as well as antioxidant capacity in the intestine and hepatopancreas of sub-adult grass carp (Ctenopharyngodon idella). Results: Dietary methionine deficiency significantly decreased percentage weight gain (PWG), feed intake, feed efficiency and protein efficiency ratio, as well as activities of hepatopancreatic glutamate-oxaloacetate transaminase and muscle glutamate-pyruvate transaminase in sub-adult grass carp (P < 0.05). Furthermore, methionine deficiency significantly reduced activities of trypsin, lipase and amylase in the intestine, Na+/K+-ATPase, alkaline phosphatase and.-glutamyl transpeptidase in three intestinal segments, and creatine kinase (CK) in the proximal intestine (P < 0.05). However, an unexplained and significant increase in CK activity in the mid intestine was associated with dietary methionine deficiency. Malondialdehyde and protein carbonyl contents in the intestine and hepatopancreas were significantly increased by methionine deficiency (P < 0.05), whereas anti-hydroxyl radical capacity in the hepatopancreas and intestine, and anti-superoxide anion capacity in the intestine, were significantly decreased by methionine deficiency (P < 0.05). Moreover, methionine deficiency significantly decreased superoxide dismutase and glutathione reductase activities, glutathione contents in the hepatopancreas and intestine, as well as glutathione peroxidase activity in the intestine (P < 0.05), whereas it significantly increased activities of catalase in the hepatopancreas and glutathione-S-transferase in the hepatopancreas and intestine (P < 0.05). Conclusions: The present results demonstrated that dietary methionine deficiency induced poor growth, and decreased digestive and absorptive function and antioxidant capacity in the hepatopancreas and intestine of sub-adult grass carp. Methionine requirements for sub-adult grass carp (450-1, 170 g) based on PWG, intestinal trypsin, and hepatopancreatic anti-hydroxyl radical activities were estimated to be 6.12 g/kg diet (21.80 g/kg protein), 6.99 g/kg diet (24.90 g/kg protein) and 5.42 g/kg diet (19.31 g/kg protein), respectively, in the presence of 1.50 g cysteine/kg (5.35 g/kg protein). Report Salmo salar Institute of Hydrobiology, Chinese Academy of Sciences: IHB OpenIR Journal of Animal Science and Biotechnology 8 1 |
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Open Polar |
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Institute of Hydrobiology, Chinese Academy of Sciences: IHB OpenIR |
op_collection_id |
ftchinacadsciihb |
language |
English |
topic |
Antioxidant status Ctenopharyngodon idella Digestive and absorptive capacities Methionine Agriculture Dairy & Animal Science TROUT ONCORHYNCHUS-MYKISS SULFUR AMINO-ACIDS SALMON SALMO-SALAR PREVENTS OXIDATIVE DAMAGE BASS DICENTRARCHUS-LABRAX LARGE YELLOW CROAKER SEMI-PURIFIED DIETS JUVENILE JIAN VAR. JIAN ENZYME-ACTIVITIES |
spellingShingle |
Antioxidant status Ctenopharyngodon idella Digestive and absorptive capacities Methionine Agriculture Dairy & Animal Science TROUT ONCORHYNCHUS-MYKISS SULFUR AMINO-ACIDS SALMON SALMO-SALAR PREVENTS OXIDATIVE DAMAGE BASS DICENTRARCHUS-LABRAX LARGE YELLOW CROAKER SEMI-PURIFIED DIETS JUVENILE JIAN VAR. JIAN ENZYME-ACTIVITIES Wu, Pei Tang, Ling Jiang, Weidan Hu, Kai Liu, Yang Jiang, Jun Kuang, Shengyao Tang, Wuneng Zhang, Yongan Zhou, Xiaoqiu Feng, Lin The relationship between dietary methionine and growth, digestion, absorption, and antioxidant status in intestinal and hepatopancreatic tissues of sub-adult grass carp (Ctenopharyngodon idella) |
topic_facet |
Antioxidant status Ctenopharyngodon idella Digestive and absorptive capacities Methionine Agriculture Dairy & Animal Science TROUT ONCORHYNCHUS-MYKISS SULFUR AMINO-ACIDS SALMON SALMO-SALAR PREVENTS OXIDATIVE DAMAGE BASS DICENTRARCHUS-LABRAX LARGE YELLOW CROAKER SEMI-PURIFIED DIETS JUVENILE JIAN VAR. JIAN ENZYME-ACTIVITIES |
description |
Background: Methionine is an essential amino acid for fish. The present study was conducted to investigate the effects of dietary methionine on growth performance, digestive and absorptive ability, as well as antioxidant capacity in the intestine and hepatopancreas of sub-adult grass carp (Ctenopharyngodon idella). Results: Dietary methionine deficiency significantly decreased percentage weight gain (PWG), feed intake, feed efficiency and protein efficiency ratio, as well as activities of hepatopancreatic glutamate-oxaloacetate transaminase and muscle glutamate-pyruvate transaminase in sub-adult grass carp (P < 0.05). Furthermore, methionine deficiency significantly reduced activities of trypsin, lipase and amylase in the intestine, Na+/K+-ATPase, alkaline phosphatase and.-glutamyl transpeptidase in three intestinal segments, and creatine kinase (CK) in the proximal intestine (P < 0.05). However, an unexplained and significant increase in CK activity in the mid intestine was associated with dietary methionine deficiency. Malondialdehyde and protein carbonyl contents in the intestine and hepatopancreas were significantly increased by methionine deficiency (P < 0.05), whereas anti-hydroxyl radical capacity in the hepatopancreas and intestine, and anti-superoxide anion capacity in the intestine, were significantly decreased by methionine deficiency (P < 0.05). Moreover, methionine deficiency significantly decreased superoxide dismutase and glutathione reductase activities, glutathione contents in the hepatopancreas and intestine, as well as glutathione peroxidase activity in the intestine (P < 0.05), whereas it significantly increased activities of catalase in the hepatopancreas and glutathione-S-transferase in the hepatopancreas and intestine (P < 0.05). Conclusions: The present results demonstrated that dietary methionine deficiency induced poor growth, and decreased digestive and absorptive function and antioxidant capacity in the hepatopancreas and intestine of sub-adult grass carp. Methionine requirements for sub-adult grass carp (450-1, 170 g) based on PWG, intestinal trypsin, and hepatopancreatic anti-hydroxyl radical activities were estimated to be 6.12 g/kg diet (21.80 g/kg protein), 6.99 g/kg diet (24.90 g/kg protein) and 5.42 g/kg diet (19.31 g/kg protein), respectively, in the presence of 1.50 g cysteine/kg (5.35 g/kg protein). |
format |
Report |
author |
Wu, Pei Tang, Ling Jiang, Weidan Hu, Kai Liu, Yang Jiang, Jun Kuang, Shengyao Tang, Wuneng Zhang, Yongan Zhou, Xiaoqiu Feng, Lin |
author_facet |
Wu, Pei Tang, Ling Jiang, Weidan Hu, Kai Liu, Yang Jiang, Jun Kuang, Shengyao Tang, Wuneng Zhang, Yongan Zhou, Xiaoqiu Feng, Lin |
author_sort |
Wu, Pei |
title |
The relationship between dietary methionine and growth, digestion, absorption, and antioxidant status in intestinal and hepatopancreatic tissues of sub-adult grass carp (Ctenopharyngodon idella) |
title_short |
The relationship between dietary methionine and growth, digestion, absorption, and antioxidant status in intestinal and hepatopancreatic tissues of sub-adult grass carp (Ctenopharyngodon idella) |
title_full |
The relationship between dietary methionine and growth, digestion, absorption, and antioxidant status in intestinal and hepatopancreatic tissues of sub-adult grass carp (Ctenopharyngodon idella) |
title_fullStr |
The relationship between dietary methionine and growth, digestion, absorption, and antioxidant status in intestinal and hepatopancreatic tissues of sub-adult grass carp (Ctenopharyngodon idella) |
title_full_unstemmed |
The relationship between dietary methionine and growth, digestion, absorption, and antioxidant status in intestinal and hepatopancreatic tissues of sub-adult grass carp (Ctenopharyngodon idella) |
title_sort |
relationship between dietary methionine and growth, digestion, absorption, and antioxidant status in intestinal and hepatopancreatic tissues of sub-adult grass carp (ctenopharyngodon idella) |
publisher |
BIOMED CENTRAL LTD |
publishDate |
2017 |
url |
http://ir.ihb.ac.cn/handle/342005/31416 http://ir.ihb.ac.cn/handle/342005/31417 https://doi.org/10.1186/s40104-017-0194-0 |
genre |
Salmo salar |
genre_facet |
Salmo salar |
op_relation |
JOURNAL OF ANIMAL SCIENCE AND BIOTECHNOLOGY http://ir.ihb.ac.cn/handle/342005/31416 http://ir.ihb.ac.cn/handle/342005/31417 doi:10.1186/s40104-017-0194-0 |
op_doi |
https://doi.org/10.1186/s40104-017-0194-0 |
container_title |
Journal of Animal Science and Biotechnology |
container_volume |
8 |
container_issue |
1 |
_version_ |
1766182776827019264 |