Dietary myo-inositol deficiency decreased the growth performances and impaired intestinal physical barrier function partly relating to nrf2, jnk, e2f4 and mlck signaling in young grass carp (Ctenopharyngodon idella)
In this study, we investigated the effects of dietary myo-inositol on the growth and intestinal physical barrier functions of young grass carp (Ctenopharyngodon idella). A total of 540 young grass carp (221.83 +/- 0.84 g) were fed six diets containing graded levels of myo-inositol (27.0, 137.9, 286....
Published in: | Fish & Shellfish Immunology |
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Language: | English |
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ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD
2017
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Online Access: | http://ir.ihb.ac.cn/handle/342005/31373 http://ir.ihb.ac.cn/handle/342005/31374 https://doi.org/10.1016/j.fsi.2017.06.032 |
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ftchinacadsciihb:oai:ir.ihb.ac.cn:342005/31374 2023-05-15T15:33:07+02:00 Dietary myo-inositol deficiency decreased the growth performances and impaired intestinal physical barrier function partly relating to nrf2, jnk, e2f4 and mlck signaling in young grass carp (Ctenopharyngodon idella) Li, Shuang-An Jiang, Wei-Dan Feng, Lin Liu, Yang Wu, Pei Jiang, Jun Kuang, Sheng-Yao Tang, Ling Tang, Wu-Neng Zhang, Yong-An Tang, Xu Shi, He-Qun Zhou, Xiao-Qiu 2017-08-01 http://ir.ihb.ac.cn/handle/342005/31373 http://ir.ihb.ac.cn/handle/342005/31374 https://doi.org/10.1016/j.fsi.2017.06.032 英语 eng ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD FISH & SHELLFISH IMMUNOLOGY http://ir.ihb.ac.cn/handle/342005/31373 http://ir.ihb.ac.cn/handle/342005/31374 doi:10.1016/j.fsi.2017.06.032 Myo-inositol Grass carp (Ctenopharyngodon idella) Intestine Antioxidant Apoptosis Proliferation Tight junction Fisheries Immunology Marine & Freshwater Biology Veterinary Sciences NF-KAPPA-B MESSENGER-RNA EXPRESSION TIGHT JUNCTION PROTEINS ANTIOXIDANT ENZYME-ACTIVITIES SALMON SALMO-SALAR AEROMONAS-HYDROPHILA IMMUNE-RESPONSE OXIDATIVE STRESS ATLANTIC SALMON ACID DEFICIENCY 期刊论文 2017 ftchinacadsciihb https://doi.org/10.1016/j.fsi.2017.06.032 2019-08-23T00:03:47Z In this study, we investigated the effects of dietary myo-inositol on the growth and intestinal physical barrier functions of young grass carp (Ctenopharyngodon idella). A total of 540 young grass carp (221.83 +/- 0.84 g) were fed six diets containing graded levels of myo-inositol (27.0, 137.9, 286.8, 438.6, 587.7 and 737.3 mg/kg) for 10 weeks. After the growth trial, fish were challenged with Aeromonas hydrophila for 14 days. The results indicated that compared with optimal myo-inositol levels, myo-inositol deficiency (27.0 mg/kg diet): (1) decreased glutathione (GSH) contents and antioxidant enzymes activities, and down-regulated the mRNA levels of antioxidant enzymes [not glutathione-S-transferase (gst) pl and gstp2] and NF-E2-related factor 2 (nrf2), whereas up-regulated the reactive oxygen species (ROS), malondialdehyde (MDA) and protein carbonyl (PC) contents, and the mRNA levels of Kelchlike-ECH-associated protein 1 (keap1) in three intestinal segments of young grass carp (P < 0.05). (2) Up-regulated cysteinyl aspartic acid-protease (caspase)-2, -3, -7, -8, -9, apoptotic protease activating factor-1 (apaf-1), Bcl2-associated X protein (bax), fas ligand (fasl), gen-activated protein kinase (p38mapk) and c-Jun N-terminal protein kinase (ink) mRNA levels, whereas down-regulated B-cell lymphoma-2 (bcl-2), inhibitor of apoptosis proteins (iap) and myeloid cell leukemia-1 (mcl-1) mRNA levels in three intestinal segments of young grass carp (P < 0.05). (3) Down-regulated mRNA levels of cell cycle proteins cyclin b, cyclin d, cyclin e and E2F transcription factor 4 (e2f4) in three intestinal segments of young grass carp (P < 0.05). (4) Down-regulated the mRNA levels of zonula occludens (zo) 1, zo-2, occludin, claudin-b, -c, -f, -3c, -7a, -7b as well as -11, and up-regulated the mRNA levels of claudin-12, -15a (not -15b) and myosin light chain kinase (mlck) in three intestinal segments of young grass carp (P < 0.05). All above data indicated that dietary myo-inositol deficiency could damage physical barrier function in three intestinal segments of fish. Finally, the myo-inositol requirements based on the percent weight gain (PWG), reactive oxygen species (ROS) contents in the proximal intestine (PI), relative mRNA levels of caspase-2 (PI), cyclin b (MI) as well as claudin-b (PI) were estimated to be 276.7, 304.1, 327.9, 416.7 and 313.2 mg/kg diet, respectively. (C) 2017 Elsevier Ltd. All rights reserved. Report Atlantic salmon Salmo salar Institute of Hydrobiology, Chinese Academy of Sciences: IHB OpenIR Fish & Shellfish Immunology 67 475 492 |
institution |
Open Polar |
collection |
Institute of Hydrobiology, Chinese Academy of Sciences: IHB OpenIR |
op_collection_id |
ftchinacadsciihb |
language |
English |
topic |
Myo-inositol Grass carp (Ctenopharyngodon idella) Intestine Antioxidant Apoptosis Proliferation Tight junction Fisheries Immunology Marine & Freshwater Biology Veterinary Sciences NF-KAPPA-B MESSENGER-RNA EXPRESSION TIGHT JUNCTION PROTEINS ANTIOXIDANT ENZYME-ACTIVITIES SALMON SALMO-SALAR AEROMONAS-HYDROPHILA IMMUNE-RESPONSE OXIDATIVE STRESS ATLANTIC SALMON ACID DEFICIENCY |
spellingShingle |
Myo-inositol Grass carp (Ctenopharyngodon idella) Intestine Antioxidant Apoptosis Proliferation Tight junction Fisheries Immunology Marine & Freshwater Biology Veterinary Sciences NF-KAPPA-B MESSENGER-RNA EXPRESSION TIGHT JUNCTION PROTEINS ANTIOXIDANT ENZYME-ACTIVITIES SALMON SALMO-SALAR AEROMONAS-HYDROPHILA IMMUNE-RESPONSE OXIDATIVE STRESS ATLANTIC SALMON ACID DEFICIENCY Li, Shuang-An Jiang, Wei-Dan Feng, Lin Liu, Yang Wu, Pei Jiang, Jun Kuang, Sheng-Yao Tang, Ling Tang, Wu-Neng Zhang, Yong-An Tang, Xu Shi, He-Qun Zhou, Xiao-Qiu Dietary myo-inositol deficiency decreased the growth performances and impaired intestinal physical barrier function partly relating to nrf2, jnk, e2f4 and mlck signaling in young grass carp (Ctenopharyngodon idella) |
topic_facet |
Myo-inositol Grass carp (Ctenopharyngodon idella) Intestine Antioxidant Apoptosis Proliferation Tight junction Fisheries Immunology Marine & Freshwater Biology Veterinary Sciences NF-KAPPA-B MESSENGER-RNA EXPRESSION TIGHT JUNCTION PROTEINS ANTIOXIDANT ENZYME-ACTIVITIES SALMON SALMO-SALAR AEROMONAS-HYDROPHILA IMMUNE-RESPONSE OXIDATIVE STRESS ATLANTIC SALMON ACID DEFICIENCY |
description |
In this study, we investigated the effects of dietary myo-inositol on the growth and intestinal physical barrier functions of young grass carp (Ctenopharyngodon idella). A total of 540 young grass carp (221.83 +/- 0.84 g) were fed six diets containing graded levels of myo-inositol (27.0, 137.9, 286.8, 438.6, 587.7 and 737.3 mg/kg) for 10 weeks. After the growth trial, fish were challenged with Aeromonas hydrophila for 14 days. The results indicated that compared with optimal myo-inositol levels, myo-inositol deficiency (27.0 mg/kg diet): (1) decreased glutathione (GSH) contents and antioxidant enzymes activities, and down-regulated the mRNA levels of antioxidant enzymes [not glutathione-S-transferase (gst) pl and gstp2] and NF-E2-related factor 2 (nrf2), whereas up-regulated the reactive oxygen species (ROS), malondialdehyde (MDA) and protein carbonyl (PC) contents, and the mRNA levels of Kelchlike-ECH-associated protein 1 (keap1) in three intestinal segments of young grass carp (P < 0.05). (2) Up-regulated cysteinyl aspartic acid-protease (caspase)-2, -3, -7, -8, -9, apoptotic protease activating factor-1 (apaf-1), Bcl2-associated X protein (bax), fas ligand (fasl), gen-activated protein kinase (p38mapk) and c-Jun N-terminal protein kinase (ink) mRNA levels, whereas down-regulated B-cell lymphoma-2 (bcl-2), inhibitor of apoptosis proteins (iap) and myeloid cell leukemia-1 (mcl-1) mRNA levels in three intestinal segments of young grass carp (P < 0.05). (3) Down-regulated mRNA levels of cell cycle proteins cyclin b, cyclin d, cyclin e and E2F transcription factor 4 (e2f4) in three intestinal segments of young grass carp (P < 0.05). (4) Down-regulated the mRNA levels of zonula occludens (zo) 1, zo-2, occludin, claudin-b, -c, -f, -3c, -7a, -7b as well as -11, and up-regulated the mRNA levels of claudin-12, -15a (not -15b) and myosin light chain kinase (mlck) in three intestinal segments of young grass carp (P < 0.05). All above data indicated that dietary myo-inositol deficiency could damage physical barrier function in three intestinal segments of fish. Finally, the myo-inositol requirements based on the percent weight gain (PWG), reactive oxygen species (ROS) contents in the proximal intestine (PI), relative mRNA levels of caspase-2 (PI), cyclin b (MI) as well as claudin-b (PI) were estimated to be 276.7, 304.1, 327.9, 416.7 and 313.2 mg/kg diet, respectively. (C) 2017 Elsevier Ltd. All rights reserved. |
format |
Report |
author |
Li, Shuang-An Jiang, Wei-Dan Feng, Lin Liu, Yang Wu, Pei Jiang, Jun Kuang, Sheng-Yao Tang, Ling Tang, Wu-Neng Zhang, Yong-An Tang, Xu Shi, He-Qun Zhou, Xiao-Qiu |
author_facet |
Li, Shuang-An Jiang, Wei-Dan Feng, Lin Liu, Yang Wu, Pei Jiang, Jun Kuang, Sheng-Yao Tang, Ling Tang, Wu-Neng Zhang, Yong-An Tang, Xu Shi, He-Qun Zhou, Xiao-Qiu |
author_sort |
Li, Shuang-An |
title |
Dietary myo-inositol deficiency decreased the growth performances and impaired intestinal physical barrier function partly relating to nrf2, jnk, e2f4 and mlck signaling in young grass carp (Ctenopharyngodon idella) |
title_short |
Dietary myo-inositol deficiency decreased the growth performances and impaired intestinal physical barrier function partly relating to nrf2, jnk, e2f4 and mlck signaling in young grass carp (Ctenopharyngodon idella) |
title_full |
Dietary myo-inositol deficiency decreased the growth performances and impaired intestinal physical barrier function partly relating to nrf2, jnk, e2f4 and mlck signaling in young grass carp (Ctenopharyngodon idella) |
title_fullStr |
Dietary myo-inositol deficiency decreased the growth performances and impaired intestinal physical barrier function partly relating to nrf2, jnk, e2f4 and mlck signaling in young grass carp (Ctenopharyngodon idella) |
title_full_unstemmed |
Dietary myo-inositol deficiency decreased the growth performances and impaired intestinal physical barrier function partly relating to nrf2, jnk, e2f4 and mlck signaling in young grass carp (Ctenopharyngodon idella) |
title_sort |
dietary myo-inositol deficiency decreased the growth performances and impaired intestinal physical barrier function partly relating to nrf2, jnk, e2f4 and mlck signaling in young grass carp (ctenopharyngodon idella) |
publisher |
ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD |
publishDate |
2017 |
url |
http://ir.ihb.ac.cn/handle/342005/31373 http://ir.ihb.ac.cn/handle/342005/31374 https://doi.org/10.1016/j.fsi.2017.06.032 |
genre |
Atlantic salmon Salmo salar |
genre_facet |
Atlantic salmon Salmo salar |
op_relation |
FISH & SHELLFISH IMMUNOLOGY http://ir.ihb.ac.cn/handle/342005/31373 http://ir.ihb.ac.cn/handle/342005/31374 doi:10.1016/j.fsi.2017.06.032 |
op_doi |
https://doi.org/10.1016/j.fsi.2017.06.032 |
container_title |
Fish & Shellfish Immunology |
container_volume |
67 |
container_start_page |
475 |
op_container_end_page |
492 |
_version_ |
1766363596874317824 |