Metagenomic insights into the abundance and composition of resistance genes in aquatic environments: Influence of stratification and geography
A global survey was performed with 122 aquatic metagenomic DNA datasets (92 lake water and 30 seawater) obtained from the Sequence Read Archive (SRA). Antibiotic resistance genes (ARGs) and metal resistance genes (MRGs) were derived from the dataset sequences via bioinformatic analysis. The relative...
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Online Access: | http://ir.ihb.ac.cn/handle/342005/31194 https://doi.org/10.1016/j.envint.2019.03.062 |
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ftchinacadsciihb:oai:ir.ihb.ac.cn:342005/31194 2023-05-15T14:02:07+02:00 Metagenomic insights into the abundance and composition of resistance genes in aquatic environments: Influence of stratification and geography Yang, Yuyi Li, Zan Song, Wenjuan Du, Linna Ye, Chen Zhao, Bo Liu, Wenzhi Deng, Danli Pan, Yongtai Lin, Hui Cao, Xinhua 2019-06-01 http://ir.ihb.ac.cn/handle/342005/31194 https://doi.org/10.1016/j.envint.2019.03.062 英语 eng PERGAMON-ELSEVIER SCIENCE LTD ENVIRONMENT INTERNATIONAL http://ir.ihb.ac.cn/handle/342005/31194 doi:10.1016/j.envint.2019.03.062 Metagenomic analysis Bacitracin resistance genes Mercury resistance genes Stratification Principal coordinates analysis Environmental Sciences & Ecology Environmental Sciences ANTIBIOTIC-RESISTANCE WASTE-WATER BACTERIAL COMMUNITY HEAVY-METALS BOHAI BAY CHINA LAKES COSELECTION RESISTOME RESERVOIR 期刊论文 2019 ftchinacadsciihb https://doi.org/10.1016/j.envint.2019.03.062 2019-07-26T00:03:57Z A global survey was performed with 122 aquatic metagenomic DNA datasets (92 lake water and 30 seawater) obtained from the Sequence Read Archive (SRA). Antibiotic resistance genes (ARGs) and metal resistance genes (MRGs) were derived from the dataset sequences via bioinformatic analysis. The relative abundances of ARGs and MRGs in lake samples were in the ranges ND (not detected)-1.34x10(0) and 1.22x10(-3) -1.98x10(-1) copies per 16S rRNA, which were higher than those in seawater samples. Among ARGs, multidrug resistance genes and bacitracin resistance genes had high relative abundances in both lake and sea water samples. Multimetal resistance genes, mercury resistance genes and copper resistance genes had the greatest relative abundance for MRGs. No significant difference was found between epilimnion and hypolimnion in abundance or the Shannon diversity index for ARGs and MRGs. Principal coordinates analysis and permutational multivariate analysis of variance (PERMANOVA) test showed that stratification and geography had significant influence on the composition of ARGs and MRGs in lakes (p < 0.05, PERMANOVA). Coastal seawater samples had significantly greater relative abundance and a higher Shannon index for both ARGs and MRGs than deep ocean and Antarctic seawater samples (p < 0.05, Kruskal-Wallis one-way ANOVA), suggesting that human activity may exert more selective pressure on ARGs and MRGs in coastal areas than those in deep ocean and Antarctic seawater. Report Antarc* Antarctic Institute of Hydrobiology, Chinese Academy of Sciences: IHB OpenIR Antarctic Environment International 127 371 380 |
institution |
Open Polar |
collection |
Institute of Hydrobiology, Chinese Academy of Sciences: IHB OpenIR |
op_collection_id |
ftchinacadsciihb |
language |
English |
topic |
Metagenomic analysis Bacitracin resistance genes Mercury resistance genes Stratification Principal coordinates analysis Environmental Sciences & Ecology Environmental Sciences ANTIBIOTIC-RESISTANCE WASTE-WATER BACTERIAL COMMUNITY HEAVY-METALS BOHAI BAY CHINA LAKES COSELECTION RESISTOME RESERVOIR |
spellingShingle |
Metagenomic analysis Bacitracin resistance genes Mercury resistance genes Stratification Principal coordinates analysis Environmental Sciences & Ecology Environmental Sciences ANTIBIOTIC-RESISTANCE WASTE-WATER BACTERIAL COMMUNITY HEAVY-METALS BOHAI BAY CHINA LAKES COSELECTION RESISTOME RESERVOIR Yang, Yuyi Li, Zan Song, Wenjuan Du, Linna Ye, Chen Zhao, Bo Liu, Wenzhi Deng, Danli Pan, Yongtai Lin, Hui Cao, Xinhua Metagenomic insights into the abundance and composition of resistance genes in aquatic environments: Influence of stratification and geography |
topic_facet |
Metagenomic analysis Bacitracin resistance genes Mercury resistance genes Stratification Principal coordinates analysis Environmental Sciences & Ecology Environmental Sciences ANTIBIOTIC-RESISTANCE WASTE-WATER BACTERIAL COMMUNITY HEAVY-METALS BOHAI BAY CHINA LAKES COSELECTION RESISTOME RESERVOIR |
description |
A global survey was performed with 122 aquatic metagenomic DNA datasets (92 lake water and 30 seawater) obtained from the Sequence Read Archive (SRA). Antibiotic resistance genes (ARGs) and metal resistance genes (MRGs) were derived from the dataset sequences via bioinformatic analysis. The relative abundances of ARGs and MRGs in lake samples were in the ranges ND (not detected)-1.34x10(0) and 1.22x10(-3) -1.98x10(-1) copies per 16S rRNA, which were higher than those in seawater samples. Among ARGs, multidrug resistance genes and bacitracin resistance genes had high relative abundances in both lake and sea water samples. Multimetal resistance genes, mercury resistance genes and copper resistance genes had the greatest relative abundance for MRGs. No significant difference was found between epilimnion and hypolimnion in abundance or the Shannon diversity index for ARGs and MRGs. Principal coordinates analysis and permutational multivariate analysis of variance (PERMANOVA) test showed that stratification and geography had significant influence on the composition of ARGs and MRGs in lakes (p < 0.05, PERMANOVA). Coastal seawater samples had significantly greater relative abundance and a higher Shannon index for both ARGs and MRGs than deep ocean and Antarctic seawater samples (p < 0.05, Kruskal-Wallis one-way ANOVA), suggesting that human activity may exert more selective pressure on ARGs and MRGs in coastal areas than those in deep ocean and Antarctic seawater. |
format |
Report |
author |
Yang, Yuyi Li, Zan Song, Wenjuan Du, Linna Ye, Chen Zhao, Bo Liu, Wenzhi Deng, Danli Pan, Yongtai Lin, Hui Cao, Xinhua |
author_facet |
Yang, Yuyi Li, Zan Song, Wenjuan Du, Linna Ye, Chen Zhao, Bo Liu, Wenzhi Deng, Danli Pan, Yongtai Lin, Hui Cao, Xinhua |
author_sort |
Yang, Yuyi |
title |
Metagenomic insights into the abundance and composition of resistance genes in aquatic environments: Influence of stratification and geography |
title_short |
Metagenomic insights into the abundance and composition of resistance genes in aquatic environments: Influence of stratification and geography |
title_full |
Metagenomic insights into the abundance and composition of resistance genes in aquatic environments: Influence of stratification and geography |
title_fullStr |
Metagenomic insights into the abundance and composition of resistance genes in aquatic environments: Influence of stratification and geography |
title_full_unstemmed |
Metagenomic insights into the abundance and composition of resistance genes in aquatic environments: Influence of stratification and geography |
title_sort |
metagenomic insights into the abundance and composition of resistance genes in aquatic environments: influence of stratification and geography |
publisher |
PERGAMON-ELSEVIER SCIENCE LTD |
publishDate |
2019 |
url |
http://ir.ihb.ac.cn/handle/342005/31194 https://doi.org/10.1016/j.envint.2019.03.062 |
geographic |
Antarctic |
geographic_facet |
Antarctic |
genre |
Antarc* Antarctic |
genre_facet |
Antarc* Antarctic |
op_relation |
ENVIRONMENT INTERNATIONAL http://ir.ihb.ac.cn/handle/342005/31194 doi:10.1016/j.envint.2019.03.062 |
op_doi |
https://doi.org/10.1016/j.envint.2019.03.062 |
container_title |
Environment International |
container_volume |
127 |
container_start_page |
371 |
op_container_end_page |
380 |
_version_ |
1766272213349040128 |