Expression analysis of immune genes in goldfish (Carassius auratus) infected with the monogenean parasite Gyrodactylus kobayashii
Heavy infection with the ectoparasite Gyrodactylus kobayashii commonly leads to high mortality of ornamental goldfish Carassius auratus. To understand the underlying mechanisms of goldfish against infection of gyrodactylids, transcription responses of immune-related genes including IL-1 beta 2, TNF...
Published in: | Fish & Shellfish Immunology |
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ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD
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Online Access: | http://ir.ihb.ac.cn/handle/342005/30439 https://doi.org/10.1016/j.fsi.2018.03.033 |
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ftchinacadsciihb:oai:ir.ihb.ac.cn:342005/30439 2023-05-15T15:32:39+02:00 Expression analysis of immune genes in goldfish (Carassius auratus) infected with the monogenean parasite Gyrodactylus kobayashii Zhou, Shun Li, Wen X. Zou, Hong Zhang, Jing Wu, Shan G. Li, Ming Wang, Gui T. 2018-06-01 http://ir.ihb.ac.cn/handle/342005/30439 https://doi.org/10.1016/j.fsi.2018.03.033 英语 eng ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD FISH & SHELLFISH IMMUNOLOGY http://ir.ihb.ac.cn/handle/342005/30439 doi:10.1016/j.fsi.2018.03.033 Gyrodactylus kobayashii Goldfish Infection Immune gene transcription Fisheries Immunology Marine & Freshwater Biology Veterinary Sciences TROUT ONCORHYNCHUS-MYKISS POECILIA-RETICULATA PETERS SALMO-SALAR L CHALLENGE INFECTIONS ATLANTIC SALMON RESPONSE GENES SKIN BULLATARUDIS GUPPIES PLATYHELMINTHES 期刊论文 2018 ftchinacadsciihb https://doi.org/10.1016/j.fsi.2018.03.033 2019-07-12T00:04:04Z Heavy infection with the ectoparasite Gyrodactylus kobayashii commonly leads to high mortality of ornamental goldfish Carassius auratus. To understand the underlying mechanisms of goldfish against infection of gyrodactylids, transcription responses of immune-related genes including IL-1 beta 2, TNF alpha 1, TNF alpha 2, IFN-gamma, TGF beta, MHC II beta, TCR beta 1 and complement factor C3 were studied by real-time quantitative PCR analysis. Significant increases of expression of inflammatory genes such as IL-1 beta 2, TNF alpha 1, TNF alpha 2 and TGF beta were detected at days 7 and 14 post-infection (pi). No significant differences of G. kobayashii load were observed in response to primary infection and re-infection. In addition, the transcript levels of genes involved in adaptive immunity such as MHC II beta and TCR beta 1 remained unchanged. Curiously, fish moderately infected with gyrodactylid showed elevated expression IL-1 beta 2, TNFa alpha 1 and TNF alpha 2. In all the gyrodactylids-infected fish, expression of complement factor C3 was consistently inhibited. The results extend current knowledge to the understanding of gyrodactylid infection in fish and support the previous findings that innate immunity is indispensable for controlling parasite infection. Report Atlantic salmon Salmo salar Institute of Hydrobiology, Chinese Academy of Sciences: IHB OpenIR Fish & Shellfish Immunology 77 40 45 |
institution |
Open Polar |
collection |
Institute of Hydrobiology, Chinese Academy of Sciences: IHB OpenIR |
op_collection_id |
ftchinacadsciihb |
language |
English |
topic |
Gyrodactylus kobayashii Goldfish Infection Immune gene transcription Fisheries Immunology Marine & Freshwater Biology Veterinary Sciences TROUT ONCORHYNCHUS-MYKISS POECILIA-RETICULATA PETERS SALMO-SALAR L CHALLENGE INFECTIONS ATLANTIC SALMON RESPONSE GENES SKIN BULLATARUDIS GUPPIES PLATYHELMINTHES |
spellingShingle |
Gyrodactylus kobayashii Goldfish Infection Immune gene transcription Fisheries Immunology Marine & Freshwater Biology Veterinary Sciences TROUT ONCORHYNCHUS-MYKISS POECILIA-RETICULATA PETERS SALMO-SALAR L CHALLENGE INFECTIONS ATLANTIC SALMON RESPONSE GENES SKIN BULLATARUDIS GUPPIES PLATYHELMINTHES Zhou, Shun Li, Wen X. Zou, Hong Zhang, Jing Wu, Shan G. Li, Ming Wang, Gui T. Expression analysis of immune genes in goldfish (Carassius auratus) infected with the monogenean parasite Gyrodactylus kobayashii |
topic_facet |
Gyrodactylus kobayashii Goldfish Infection Immune gene transcription Fisheries Immunology Marine & Freshwater Biology Veterinary Sciences TROUT ONCORHYNCHUS-MYKISS POECILIA-RETICULATA PETERS SALMO-SALAR L CHALLENGE INFECTIONS ATLANTIC SALMON RESPONSE GENES SKIN BULLATARUDIS GUPPIES PLATYHELMINTHES |
description |
Heavy infection with the ectoparasite Gyrodactylus kobayashii commonly leads to high mortality of ornamental goldfish Carassius auratus. To understand the underlying mechanisms of goldfish against infection of gyrodactylids, transcription responses of immune-related genes including IL-1 beta 2, TNF alpha 1, TNF alpha 2, IFN-gamma, TGF beta, MHC II beta, TCR beta 1 and complement factor C3 were studied by real-time quantitative PCR analysis. Significant increases of expression of inflammatory genes such as IL-1 beta 2, TNF alpha 1, TNF alpha 2 and TGF beta were detected at days 7 and 14 post-infection (pi). No significant differences of G. kobayashii load were observed in response to primary infection and re-infection. In addition, the transcript levels of genes involved in adaptive immunity such as MHC II beta and TCR beta 1 remained unchanged. Curiously, fish moderately infected with gyrodactylid showed elevated expression IL-1 beta 2, TNFa alpha 1 and TNF alpha 2. In all the gyrodactylids-infected fish, expression of complement factor C3 was consistently inhibited. The results extend current knowledge to the understanding of gyrodactylid infection in fish and support the previous findings that innate immunity is indispensable for controlling parasite infection. |
format |
Report |
author |
Zhou, Shun Li, Wen X. Zou, Hong Zhang, Jing Wu, Shan G. Li, Ming Wang, Gui T. |
author_facet |
Zhou, Shun Li, Wen X. Zou, Hong Zhang, Jing Wu, Shan G. Li, Ming Wang, Gui T. |
author_sort |
Zhou, Shun |
title |
Expression analysis of immune genes in goldfish (Carassius auratus) infected with the monogenean parasite Gyrodactylus kobayashii |
title_short |
Expression analysis of immune genes in goldfish (Carassius auratus) infected with the monogenean parasite Gyrodactylus kobayashii |
title_full |
Expression analysis of immune genes in goldfish (Carassius auratus) infected with the monogenean parasite Gyrodactylus kobayashii |
title_fullStr |
Expression analysis of immune genes in goldfish (Carassius auratus) infected with the monogenean parasite Gyrodactylus kobayashii |
title_full_unstemmed |
Expression analysis of immune genes in goldfish (Carassius auratus) infected with the monogenean parasite Gyrodactylus kobayashii |
title_sort |
expression analysis of immune genes in goldfish (carassius auratus) infected with the monogenean parasite gyrodactylus kobayashii |
publisher |
ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD |
publishDate |
2018 |
url |
http://ir.ihb.ac.cn/handle/342005/30439 https://doi.org/10.1016/j.fsi.2018.03.033 |
genre |
Atlantic salmon Salmo salar |
genre_facet |
Atlantic salmon Salmo salar |
op_relation |
FISH & SHELLFISH IMMUNOLOGY http://ir.ihb.ac.cn/handle/342005/30439 doi:10.1016/j.fsi.2018.03.033 |
op_doi |
https://doi.org/10.1016/j.fsi.2018.03.033 |
container_title |
Fish & Shellfish Immunology |
container_volume |
77 |
container_start_page |
40 |
op_container_end_page |
45 |
_version_ |
1766363145024045056 |