Dietary arginine supplementation mitigates the soybean meal induced enteropathy in juvenile turbot, Scophthalmus maximus L.
A 12-week feeding trial was conducted to investigate the protective effects of arginine on the intestinal health of juvenile turbot (Scophthalmus maximus L.) fed high doses of dietary soybean meal. Sextuple replicates of 30 fish were fed each of four isonitrogenous and isolipidic experimental diets:...
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WILEY
2018
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Online Access: | http://ir.ihb.ac.cn/handle/342005/30337 http://ir.ihb.ac.cn/handle/342005/30338 https://doi.org/10.1111/are.13608 |
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ftchinacadsciihb:oai:ir.ihb.ac.cn:342005/30338 2023-05-15T18:10:01+02:00 Dietary arginine supplementation mitigates the soybean meal induced enteropathy in juvenile turbot, Scophthalmus maximus L. Chen, Zhichu Liu, Yang Li, Yanxian Yang, Pei Hu, Haibin Yu, Guijuan Ai, Qinghui Xu, Wei Zhang, Wenbing Zhang, Yongan Zhang, Yanjiao Mai, Kangsen 2018-04-01 http://ir.ihb.ac.cn/handle/342005/30337 http://ir.ihb.ac.cn/handle/342005/30338 https://doi.org/10.1111/are.13608 英语 eng WILEY AQUACULTURE RESEARCH http://ir.ihb.ac.cn/handle/342005/30337 http://ir.ihb.ac.cn/handle/342005/30338 doi:10.1111/are.13608 arginine enteropathy intestinal mucosal barrier soybean meal turbot Fisheries MESSENGER-RNA EXPRESSION SALMON SALMO-SALAR CARPIO VAR. JIAN NF-KAPPA-B GROWTH-PERFORMANCE IMMUNE-RESPONSES EXPERIMENTAL COLITIS DISTAL INTESTINE BARRIER FUNCTION GENE-EXPRESSION 期刊论文 2018 ftchinacadsciihb https://doi.org/10.1111/are.13608 2019-08-23T00:03:43Z A 12-week feeding trial was conducted to investigate the protective effects of arginine on the intestinal health of juvenile turbot (Scophthalmus maximus L.) fed high doses of dietary soybean meal. Sextuple replicates of 30 fish were fed each of four isonitrogenous and isolipidic experimental diets: fish-meal-based diet (FM); FM with 40% fish meal protein replaced by soybean meal protein (SBM); SBM+1% arginine (ARG1) and SBM+2% arginine (ARG2). Turbot-fed SBM showed typical soybean meal-induced enteropathy, characterized by an increase in the thickness of lamina propria, as well as significant decreases in the absorptive surface and activities of intestinal brush border membrane enzymes and Na+, K+-ATPase. On the contrary, fish-fed ARG1 showed enhanced intestinal mucosal barrier function in terms of the enhanced gene expression of anti-inflammatory cytokine and barrier-forming tight junction proteins, as well as depressed gene expression of pro-inflammatory cytokines and pore-forming tight junction proteins. Fish-fed AGR2 showed intermediate intestinal performances between SBM and AGR1. Dietary arginine (1%) also significantly regulated the expression of AMP-activated protein kinase alpha 1 (AMPK alpha 1), myosin light chain kinase (MLCK) and nuclear transcription factor-kappa B p65 (NF-kappa B p65), and these regulations correlated well with its regulations on intestinal mucosal barrier related genes at all sampling time-points. In conclusion, arginine supplementation (1%) in diet for turbot mitigated the soybean meal-induced enteropathy by enhancing the intestinal mucosal barrier function. The activation of AMPK alpha 1 signalling molecule as well as the suppression of NF-kappa B p65 and MLCK signalling molecules may mediate the beneficial effects of arginine. Report Salmo salar Scophthalmus maximus Turbot Institute of Hydrobiology, Chinese Academy of Sciences: IHB OpenIR Aquaculture Research 49 4 1535 1545 |
institution |
Open Polar |
collection |
Institute of Hydrobiology, Chinese Academy of Sciences: IHB OpenIR |
op_collection_id |
ftchinacadsciihb |
language |
English |
topic |
arginine enteropathy intestinal mucosal barrier soybean meal turbot Fisheries MESSENGER-RNA EXPRESSION SALMON SALMO-SALAR CARPIO VAR. JIAN NF-KAPPA-B GROWTH-PERFORMANCE IMMUNE-RESPONSES EXPERIMENTAL COLITIS DISTAL INTESTINE BARRIER FUNCTION GENE-EXPRESSION |
spellingShingle |
arginine enteropathy intestinal mucosal barrier soybean meal turbot Fisheries MESSENGER-RNA EXPRESSION SALMON SALMO-SALAR CARPIO VAR. JIAN NF-KAPPA-B GROWTH-PERFORMANCE IMMUNE-RESPONSES EXPERIMENTAL COLITIS DISTAL INTESTINE BARRIER FUNCTION GENE-EXPRESSION Chen, Zhichu Liu, Yang Li, Yanxian Yang, Pei Hu, Haibin Yu, Guijuan Ai, Qinghui Xu, Wei Zhang, Wenbing Zhang, Yongan Zhang, Yanjiao Mai, Kangsen Dietary arginine supplementation mitigates the soybean meal induced enteropathy in juvenile turbot, Scophthalmus maximus L. |
topic_facet |
arginine enteropathy intestinal mucosal barrier soybean meal turbot Fisheries MESSENGER-RNA EXPRESSION SALMON SALMO-SALAR CARPIO VAR. JIAN NF-KAPPA-B GROWTH-PERFORMANCE IMMUNE-RESPONSES EXPERIMENTAL COLITIS DISTAL INTESTINE BARRIER FUNCTION GENE-EXPRESSION |
description |
A 12-week feeding trial was conducted to investigate the protective effects of arginine on the intestinal health of juvenile turbot (Scophthalmus maximus L.) fed high doses of dietary soybean meal. Sextuple replicates of 30 fish were fed each of four isonitrogenous and isolipidic experimental diets: fish-meal-based diet (FM); FM with 40% fish meal protein replaced by soybean meal protein (SBM); SBM+1% arginine (ARG1) and SBM+2% arginine (ARG2). Turbot-fed SBM showed typical soybean meal-induced enteropathy, characterized by an increase in the thickness of lamina propria, as well as significant decreases in the absorptive surface and activities of intestinal brush border membrane enzymes and Na+, K+-ATPase. On the contrary, fish-fed ARG1 showed enhanced intestinal mucosal barrier function in terms of the enhanced gene expression of anti-inflammatory cytokine and barrier-forming tight junction proteins, as well as depressed gene expression of pro-inflammatory cytokines and pore-forming tight junction proteins. Fish-fed AGR2 showed intermediate intestinal performances between SBM and AGR1. Dietary arginine (1%) also significantly regulated the expression of AMP-activated protein kinase alpha 1 (AMPK alpha 1), myosin light chain kinase (MLCK) and nuclear transcription factor-kappa B p65 (NF-kappa B p65), and these regulations correlated well with its regulations on intestinal mucosal barrier related genes at all sampling time-points. In conclusion, arginine supplementation (1%) in diet for turbot mitigated the soybean meal-induced enteropathy by enhancing the intestinal mucosal barrier function. The activation of AMPK alpha 1 signalling molecule as well as the suppression of NF-kappa B p65 and MLCK signalling molecules may mediate the beneficial effects of arginine. |
format |
Report |
author |
Chen, Zhichu Liu, Yang Li, Yanxian Yang, Pei Hu, Haibin Yu, Guijuan Ai, Qinghui Xu, Wei Zhang, Wenbing Zhang, Yongan Zhang, Yanjiao Mai, Kangsen |
author_facet |
Chen, Zhichu Liu, Yang Li, Yanxian Yang, Pei Hu, Haibin Yu, Guijuan Ai, Qinghui Xu, Wei Zhang, Wenbing Zhang, Yongan Zhang, Yanjiao Mai, Kangsen |
author_sort |
Chen, Zhichu |
title |
Dietary arginine supplementation mitigates the soybean meal induced enteropathy in juvenile turbot, Scophthalmus maximus L. |
title_short |
Dietary arginine supplementation mitigates the soybean meal induced enteropathy in juvenile turbot, Scophthalmus maximus L. |
title_full |
Dietary arginine supplementation mitigates the soybean meal induced enteropathy in juvenile turbot, Scophthalmus maximus L. |
title_fullStr |
Dietary arginine supplementation mitigates the soybean meal induced enteropathy in juvenile turbot, Scophthalmus maximus L. |
title_full_unstemmed |
Dietary arginine supplementation mitigates the soybean meal induced enteropathy in juvenile turbot, Scophthalmus maximus L. |
title_sort |
dietary arginine supplementation mitigates the soybean meal induced enteropathy in juvenile turbot, scophthalmus maximus l. |
publisher |
WILEY |
publishDate |
2018 |
url |
http://ir.ihb.ac.cn/handle/342005/30337 http://ir.ihb.ac.cn/handle/342005/30338 https://doi.org/10.1111/are.13608 |
genre |
Salmo salar Scophthalmus maximus Turbot |
genre_facet |
Salmo salar Scophthalmus maximus Turbot |
op_relation |
AQUACULTURE RESEARCH http://ir.ihb.ac.cn/handle/342005/30337 http://ir.ihb.ac.cn/handle/342005/30338 doi:10.1111/are.13608 |
op_doi |
https://doi.org/10.1111/are.13608 |
container_title |
Aquaculture Research |
container_volume |
49 |
container_issue |
4 |
container_start_page |
1535 |
op_container_end_page |
1545 |
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1766182732037095424 |