Dietary pyridoxine deficiency reduced growth performance and impaired intestinal immune function associated with TOR and NF-kappa B signalling of young grass carp (Ctenopharyngodon idella)
The objective of this study was to evaluate the effects of dietary pyridoxine (PN) deficiency on growth performance, intestinal immune function and the potential regulation mechanisms in young grass carp (Ctenopharyngodon idella). Fish were fed six diets containing graded levels of PN (0.12-7.48 mg/...
Published in: | Fish & Shellfish Immunology |
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Language: | English |
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ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD
2017
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Online Access: | http://ir.ihb.ac.cn/handle/342005/30185 http://ir.ihb.ac.cn/handle/342005/30186 https://doi.org/10.1016/j.fsi.2017.09.055 |
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ftchinacadsciihb:oai:ir.ihb.ac.cn:342005/30186 2023-05-15T18:10:03+02:00 Dietary pyridoxine deficiency reduced growth performance and impaired intestinal immune function associated with TOR and NF-kappa B signalling of young grass carp (Ctenopharyngodon idella) Zheng, Xin Feng, Lin Jiang, Wei-Dan Wu, Pei Liu, Yang Jiang, Jun Kuang, Sheng-Yao Tang, Ling Tang, Wu-Neng Zhang, Yong-An Zhou, Xiao-Qiu 2017-11-01 http://ir.ihb.ac.cn/handle/342005/30185 http://ir.ihb.ac.cn/handle/342005/30186 https://doi.org/10.1016/j.fsi.2017.09.055 英语 eng ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD FISH & SHELLFISH IMMUNOLOGY http://ir.ihb.ac.cn/handle/342005/30185 http://ir.ihb.ac.cn/handle/342005/30186 doi:10.1016/j.fsi.2017.09.055 Pyridoxine deficiency Intestinal immune TOR signalling NF-kappa B signalling Grass carp (Ctenopharyngodon idella) Fisheries Immunology Marine & Freshwater Biology Veterinary Sciences AEROMONAS-HYDROPHILA INFECTION MESSENGER-RNA EXPRESSION TIGHT JUNCTION PROTEINS SALMON SALMO-SALAR VITAMIN-B-6 SUPPLEMENTATION INCREASES PHYSICAL BARRIER FUNCTION JUVENILE JIAN CARP CYPRINUS-CARPIO VAR. JIAN DISEASE RESISTANCE 期刊论文 2017 ftchinacadsciihb https://doi.org/10.1016/j.fsi.2017.09.055 2019-08-30T00:04:01Z The objective of this study was to evaluate the effects of dietary pyridoxine (PN) deficiency on growth performance, intestinal immune function and the potential regulation mechanisms in young grass carp (Ctenopharyngodon idella). Fish were fed six diets containing graded levels of PN (0.12-7.48 mg/kg) for 70 days. After that, a challenge test was conducted by infection of Aeromonas hydrophila for 14 days. The results showed that compared with the optimal PN level, PN deficiency: (1) reduced the production of innate immune components such as lysozyme (LZ), acid phosphatase (ACP), complements and antimicrobial peptides and adaptive immune components such as immunoglobulins in three intestinal segments of young grass carp (P < 0.05); (2) down-regulated the mRNA levels of anti-inflammatory cytokines such as transforming growth factor beta (TGF-beta), interleukin 4/13A (IL-4/13A) (rather than IL-4/13B), IL-10 and IL-11 partly relating to target of rapamycin (TOR) signalling [TOR/ribosomal protein S6 kinases 1 (S6K1) and eIF4E-binding proteins (4E-BP)] in three intestinal segments of young grass carp; (3) up-regulated the mRNA levels of pro-inflammatory cytokines such as tumour necrosis factor alpha (TNF-alpha) [not in the proximal intestine (PI) and distal intestine (DI)], IL-1 beta, IL-6, IL-8, IL-12p35, IL-12p40, IL-15 and IL-17D [(rather than interferon gamma 2 (IFN-gamma 2)] partly relating to nuclear factor kappa B (NF-kappa B) signalling [I kappa B kinase beta (IKK beta) and IKK gamma/inhibitor of kappa B alpha (I kappa B alpha)/NF-kappa B (p65 and c-Rel)] in three intestinal segments of young grass carp. These results suggest that PN deficiency could impair the intestinal immune function, and the potential regulation mechanisms were partly associated with TOR and NF-kappa B signalling pathways. In addition, based on percent weight gain (PWG), the ability against enteritis and LZ activity, the dietary PN requirements for young grass carp were estimated to be 4.43, 4.75 and 5.07 mg/kg diet, respectively. (C) 2017 Published by Elsevier Ltd. Report Salmo salar Institute of Hydrobiology, Chinese Academy of Sciences: IHB OpenIR Fish & Shellfish Immunology 70 682 700 |
institution |
Open Polar |
collection |
Institute of Hydrobiology, Chinese Academy of Sciences: IHB OpenIR |
op_collection_id |
ftchinacadsciihb |
language |
English |
topic |
Pyridoxine deficiency Intestinal immune TOR signalling NF-kappa B signalling Grass carp (Ctenopharyngodon idella) Fisheries Immunology Marine & Freshwater Biology Veterinary Sciences AEROMONAS-HYDROPHILA INFECTION MESSENGER-RNA EXPRESSION TIGHT JUNCTION PROTEINS SALMON SALMO-SALAR VITAMIN-B-6 SUPPLEMENTATION INCREASES PHYSICAL BARRIER FUNCTION JUVENILE JIAN CARP CYPRINUS-CARPIO VAR. JIAN DISEASE RESISTANCE |
spellingShingle |
Pyridoxine deficiency Intestinal immune TOR signalling NF-kappa B signalling Grass carp (Ctenopharyngodon idella) Fisheries Immunology Marine & Freshwater Biology Veterinary Sciences AEROMONAS-HYDROPHILA INFECTION MESSENGER-RNA EXPRESSION TIGHT JUNCTION PROTEINS SALMON SALMO-SALAR VITAMIN-B-6 SUPPLEMENTATION INCREASES PHYSICAL BARRIER FUNCTION JUVENILE JIAN CARP CYPRINUS-CARPIO VAR. JIAN DISEASE RESISTANCE Zheng, Xin Feng, Lin Jiang, Wei-Dan Wu, Pei Liu, Yang Jiang, Jun Kuang, Sheng-Yao Tang, Ling Tang, Wu-Neng Zhang, Yong-An Zhou, Xiao-Qiu Dietary pyridoxine deficiency reduced growth performance and impaired intestinal immune function associated with TOR and NF-kappa B signalling of young grass carp (Ctenopharyngodon idella) |
topic_facet |
Pyridoxine deficiency Intestinal immune TOR signalling NF-kappa B signalling Grass carp (Ctenopharyngodon idella) Fisheries Immunology Marine & Freshwater Biology Veterinary Sciences AEROMONAS-HYDROPHILA INFECTION MESSENGER-RNA EXPRESSION TIGHT JUNCTION PROTEINS SALMON SALMO-SALAR VITAMIN-B-6 SUPPLEMENTATION INCREASES PHYSICAL BARRIER FUNCTION JUVENILE JIAN CARP CYPRINUS-CARPIO VAR. JIAN DISEASE RESISTANCE |
description |
The objective of this study was to evaluate the effects of dietary pyridoxine (PN) deficiency on growth performance, intestinal immune function and the potential regulation mechanisms in young grass carp (Ctenopharyngodon idella). Fish were fed six diets containing graded levels of PN (0.12-7.48 mg/kg) for 70 days. After that, a challenge test was conducted by infection of Aeromonas hydrophila for 14 days. The results showed that compared with the optimal PN level, PN deficiency: (1) reduced the production of innate immune components such as lysozyme (LZ), acid phosphatase (ACP), complements and antimicrobial peptides and adaptive immune components such as immunoglobulins in three intestinal segments of young grass carp (P < 0.05); (2) down-regulated the mRNA levels of anti-inflammatory cytokines such as transforming growth factor beta (TGF-beta), interleukin 4/13A (IL-4/13A) (rather than IL-4/13B), IL-10 and IL-11 partly relating to target of rapamycin (TOR) signalling [TOR/ribosomal protein S6 kinases 1 (S6K1) and eIF4E-binding proteins (4E-BP)] in three intestinal segments of young grass carp; (3) up-regulated the mRNA levels of pro-inflammatory cytokines such as tumour necrosis factor alpha (TNF-alpha) [not in the proximal intestine (PI) and distal intestine (DI)], IL-1 beta, IL-6, IL-8, IL-12p35, IL-12p40, IL-15 and IL-17D [(rather than interferon gamma 2 (IFN-gamma 2)] partly relating to nuclear factor kappa B (NF-kappa B) signalling [I kappa B kinase beta (IKK beta) and IKK gamma/inhibitor of kappa B alpha (I kappa B alpha)/NF-kappa B (p65 and c-Rel)] in three intestinal segments of young grass carp. These results suggest that PN deficiency could impair the intestinal immune function, and the potential regulation mechanisms were partly associated with TOR and NF-kappa B signalling pathways. In addition, based on percent weight gain (PWG), the ability against enteritis and LZ activity, the dietary PN requirements for young grass carp were estimated to be 4.43, 4.75 and 5.07 mg/kg diet, respectively. (C) 2017 Published by Elsevier Ltd. |
format |
Report |
author |
Zheng, Xin Feng, Lin Jiang, Wei-Dan Wu, Pei Liu, Yang Jiang, Jun Kuang, Sheng-Yao Tang, Ling Tang, Wu-Neng Zhang, Yong-An Zhou, Xiao-Qiu |
author_facet |
Zheng, Xin Feng, Lin Jiang, Wei-Dan Wu, Pei Liu, Yang Jiang, Jun Kuang, Sheng-Yao Tang, Ling Tang, Wu-Neng Zhang, Yong-An Zhou, Xiao-Qiu |
author_sort |
Zheng, Xin |
title |
Dietary pyridoxine deficiency reduced growth performance and impaired intestinal immune function associated with TOR and NF-kappa B signalling of young grass carp (Ctenopharyngodon idella) |
title_short |
Dietary pyridoxine deficiency reduced growth performance and impaired intestinal immune function associated with TOR and NF-kappa B signalling of young grass carp (Ctenopharyngodon idella) |
title_full |
Dietary pyridoxine deficiency reduced growth performance and impaired intestinal immune function associated with TOR and NF-kappa B signalling of young grass carp (Ctenopharyngodon idella) |
title_fullStr |
Dietary pyridoxine deficiency reduced growth performance and impaired intestinal immune function associated with TOR and NF-kappa B signalling of young grass carp (Ctenopharyngodon idella) |
title_full_unstemmed |
Dietary pyridoxine deficiency reduced growth performance and impaired intestinal immune function associated with TOR and NF-kappa B signalling of young grass carp (Ctenopharyngodon idella) |
title_sort |
dietary pyridoxine deficiency reduced growth performance and impaired intestinal immune function associated with tor and nf-kappa b signalling of young grass carp (ctenopharyngodon idella) |
publisher |
ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD |
publishDate |
2017 |
url |
http://ir.ihb.ac.cn/handle/342005/30185 http://ir.ihb.ac.cn/handle/342005/30186 https://doi.org/10.1016/j.fsi.2017.09.055 |
genre |
Salmo salar |
genre_facet |
Salmo salar |
op_relation |
FISH & SHELLFISH IMMUNOLOGY http://ir.ihb.ac.cn/handle/342005/30185 http://ir.ihb.ac.cn/handle/342005/30186 doi:10.1016/j.fsi.2017.09.055 |
op_doi |
https://doi.org/10.1016/j.fsi.2017.09.055 |
container_title |
Fish & Shellfish Immunology |
container_volume |
70 |
container_start_page |
682 |
op_container_end_page |
700 |
_version_ |
1766182786482307072 |