Dietary stachyose altered the intestinal microbiota profile and improved the intestinal mucosal barrier function of juvenile turbot, Scophthalmus maximus L.
Recent studies have revealed the beneficial effects of stachyose on intestinal histology and digestive function of fish. However, a comprehensive understanding of stachyose's impact on intestinal health of fish remains unclear, limiting its use in aqua-feed. In the present study, a 12-week feed...
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Online Access: | http://ir.ihb.ac.cn/handle/342005/29848 http://ir.ihb.ac.cn/handle/342005/29849 https://doi.org/10.1016/j.aquaculture.2017.12.014 |
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ftchinacadsciihb:oai:ir.ihb.ac.cn:342005/29849 2023-05-15T15:32:58+02:00 Dietary stachyose altered the intestinal microbiota profile and improved the intestinal mucosal barrier function of juvenile turbot, Scophthalmus maximus L. Yang, Pei Hu, Haibin Liu, Yang Li, Yanxian Ai, Qinghui Xu, Wei Zhang, Wenbing Zhang, Yongan Zhang, Yanjiao Mai, Kangsen 2018-02-03 http://ir.ihb.ac.cn/handle/342005/29848 http://ir.ihb.ac.cn/handle/342005/29849 https://doi.org/10.1016/j.aquaculture.2017.12.014 英语 eng ELSEVIER SCIENCE BV AQUACULTURE http://ir.ihb.ac.cn/handle/342005/29848 http://ir.ihb.ac.cn/handle/342005/29849 doi:10.1016/j.aquaculture.2017.12.014 Stachyose Intestinal microbiota Intestinal tight junction Turbot Fisheries Marine & Freshwater Biology SALMON SALMO-SALAR ALPHA-GALACTO-OLIGOSACCHARIDES CARP CTENOPHARYNGODON-IDELLA DIGESTIVE ENZYME-ACTIVITIES MUCIN GENE-EXPRESSION GUT MICROBIOTA ATLANTIC SALMON GROWTH-PERFORMANCE FISH BACTERIA 期刊论文 2018 ftchinacadsciihb https://doi.org/10.1016/j.aquaculture.2017.12.014 2019-08-30T00:04:01Z Recent studies have revealed the beneficial effects of stachyose on intestinal histology and digestive function of fish. However, a comprehensive understanding of stachyose's impact on intestinal health of fish remains unclear, limiting its use in aqua-feed. In the present study, a 12-week feeding trial was conducted to investigate the effects of dietary stachyose on intestinal microbiota and mucosal barrier function of turbot (S. maximus L). Three isonitrogenous and isolipidic experimental diets were formulated to contain 0%, 1.25% and 5% stachyose, respectively. Sequencing of bacterial 16s rRNA V-4 region indicated that dietary stachyose altered the intestinal adherent microbiota profile, which was supported by the diet-cluster of PCA, PCoA, beta diversity heatmap and phylogenetic tree. LEfSe and MetaStat analysis indicated that both 1.25% and 5% dietary stachyose significantly elevated the abundance of intestinal cellulose-degrading bacteria. However, the higher level of stachyose (5%) increased the abundance of intestinal beneficial bacteria as well as that of potential pathogenic bacteria. Moreover, 1.25% dietary stachyose significantly up-regulated the genes expression of occludin, claudin-3, and ZO-1, and down-regulated the gene expression of claudin-like in the intestine (P<0.05). Dietary stachyose at 5% significantly increased mucin-2 secretion and the gene expression of ZO-1, while significantly decreased the gene expression of claudin-like in the intestine (P<0.05). Collectively, our study showed that dietary stachyose supplementation could favorably modulate the profile of intestinal microbiota and enhance the intestinal mucosal barrier function in juvenile turbot. Stachyose showed promising potential of being used as prebiotic in diet for enhancing the intestinal health of turbot. Report Atlantic salmon Salmo salar Scophthalmus maximus Turbot Institute of Hydrobiology, Chinese Academy of Sciences: IHB OpenIR Aquaculture 486 98 106 |
institution |
Open Polar |
collection |
Institute of Hydrobiology, Chinese Academy of Sciences: IHB OpenIR |
op_collection_id |
ftchinacadsciihb |
language |
English |
topic |
Stachyose Intestinal microbiota Intestinal tight junction Turbot Fisheries Marine & Freshwater Biology SALMON SALMO-SALAR ALPHA-GALACTO-OLIGOSACCHARIDES CARP CTENOPHARYNGODON-IDELLA DIGESTIVE ENZYME-ACTIVITIES MUCIN GENE-EXPRESSION GUT MICROBIOTA ATLANTIC SALMON GROWTH-PERFORMANCE FISH BACTERIA |
spellingShingle |
Stachyose Intestinal microbiota Intestinal tight junction Turbot Fisheries Marine & Freshwater Biology SALMON SALMO-SALAR ALPHA-GALACTO-OLIGOSACCHARIDES CARP CTENOPHARYNGODON-IDELLA DIGESTIVE ENZYME-ACTIVITIES MUCIN GENE-EXPRESSION GUT MICROBIOTA ATLANTIC SALMON GROWTH-PERFORMANCE FISH BACTERIA Yang, Pei Hu, Haibin Liu, Yang Li, Yanxian Ai, Qinghui Xu, Wei Zhang, Wenbing Zhang, Yongan Zhang, Yanjiao Mai, Kangsen Dietary stachyose altered the intestinal microbiota profile and improved the intestinal mucosal barrier function of juvenile turbot, Scophthalmus maximus L. |
topic_facet |
Stachyose Intestinal microbiota Intestinal tight junction Turbot Fisheries Marine & Freshwater Biology SALMON SALMO-SALAR ALPHA-GALACTO-OLIGOSACCHARIDES CARP CTENOPHARYNGODON-IDELLA DIGESTIVE ENZYME-ACTIVITIES MUCIN GENE-EXPRESSION GUT MICROBIOTA ATLANTIC SALMON GROWTH-PERFORMANCE FISH BACTERIA |
description |
Recent studies have revealed the beneficial effects of stachyose on intestinal histology and digestive function of fish. However, a comprehensive understanding of stachyose's impact on intestinal health of fish remains unclear, limiting its use in aqua-feed. In the present study, a 12-week feeding trial was conducted to investigate the effects of dietary stachyose on intestinal microbiota and mucosal barrier function of turbot (S. maximus L). Three isonitrogenous and isolipidic experimental diets were formulated to contain 0%, 1.25% and 5% stachyose, respectively. Sequencing of bacterial 16s rRNA V-4 region indicated that dietary stachyose altered the intestinal adherent microbiota profile, which was supported by the diet-cluster of PCA, PCoA, beta diversity heatmap and phylogenetic tree. LEfSe and MetaStat analysis indicated that both 1.25% and 5% dietary stachyose significantly elevated the abundance of intestinal cellulose-degrading bacteria. However, the higher level of stachyose (5%) increased the abundance of intestinal beneficial bacteria as well as that of potential pathogenic bacteria. Moreover, 1.25% dietary stachyose significantly up-regulated the genes expression of occludin, claudin-3, and ZO-1, and down-regulated the gene expression of claudin-like in the intestine (P<0.05). Dietary stachyose at 5% significantly increased mucin-2 secretion and the gene expression of ZO-1, while significantly decreased the gene expression of claudin-like in the intestine (P<0.05). Collectively, our study showed that dietary stachyose supplementation could favorably modulate the profile of intestinal microbiota and enhance the intestinal mucosal barrier function in juvenile turbot. Stachyose showed promising potential of being used as prebiotic in diet for enhancing the intestinal health of turbot. |
format |
Report |
author |
Yang, Pei Hu, Haibin Liu, Yang Li, Yanxian Ai, Qinghui Xu, Wei Zhang, Wenbing Zhang, Yongan Zhang, Yanjiao Mai, Kangsen |
author_facet |
Yang, Pei Hu, Haibin Liu, Yang Li, Yanxian Ai, Qinghui Xu, Wei Zhang, Wenbing Zhang, Yongan Zhang, Yanjiao Mai, Kangsen |
author_sort |
Yang, Pei |
title |
Dietary stachyose altered the intestinal microbiota profile and improved the intestinal mucosal barrier function of juvenile turbot, Scophthalmus maximus L. |
title_short |
Dietary stachyose altered the intestinal microbiota profile and improved the intestinal mucosal barrier function of juvenile turbot, Scophthalmus maximus L. |
title_full |
Dietary stachyose altered the intestinal microbiota profile and improved the intestinal mucosal barrier function of juvenile turbot, Scophthalmus maximus L. |
title_fullStr |
Dietary stachyose altered the intestinal microbiota profile and improved the intestinal mucosal barrier function of juvenile turbot, Scophthalmus maximus L. |
title_full_unstemmed |
Dietary stachyose altered the intestinal microbiota profile and improved the intestinal mucosal barrier function of juvenile turbot, Scophthalmus maximus L. |
title_sort |
dietary stachyose altered the intestinal microbiota profile and improved the intestinal mucosal barrier function of juvenile turbot, scophthalmus maximus l. |
publisher |
ELSEVIER SCIENCE BV |
publishDate |
2018 |
url |
http://ir.ihb.ac.cn/handle/342005/29848 http://ir.ihb.ac.cn/handle/342005/29849 https://doi.org/10.1016/j.aquaculture.2017.12.014 |
genre |
Atlantic salmon Salmo salar Scophthalmus maximus Turbot |
genre_facet |
Atlantic salmon Salmo salar Scophthalmus maximus Turbot |
op_relation |
AQUACULTURE http://ir.ihb.ac.cn/handle/342005/29848 http://ir.ihb.ac.cn/handle/342005/29849 doi:10.1016/j.aquaculture.2017.12.014 |
op_doi |
https://doi.org/10.1016/j.aquaculture.2017.12.014 |
container_title |
Aquaculture |
container_volume |
486 |
container_start_page |
98 |
op_container_end_page |
106 |
_version_ |
1766363449609158656 |