Comparative study of the immunoprotective effect of two DNA vaccines against grass carp reovirus
Grass carp reovirus II (GCRV II) causes severe hemorrhagic disease with high mortality in grass carp, Cyenopharyngodon idellus. DNA vaccination has been proven to be a very effective method in conferring protection against fish viruses. However, DNA vaccines for GCRV II have not yet been conducted o...
Published in: | Fish & Shellfish Immunology |
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Language: | English |
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ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD
2018
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Online Access: | http://ir.ihb.ac.cn/handle/342005/29573 http://ir.ihb.ac.cn/handle/342005/29574 https://doi.org/10.1016/j.fsi.2018.01.047 |
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ftchinacadsciihb:oai:ir.ihb.ac.cn:342005/29574 2023-05-15T18:10:02+02:00 Comparative study of the immunoprotective effect of two DNA vaccines against grass carp reovirus Chen, Dan-Dan Yao, Yuan-Yuan Cui, Zheng-Wei Zhang, Xiang-Yang Peng, Kai-Song Guo, Xia Wang, Biao Zhou, Yuan-Yuan Li, Shun Wu, Nan Zhang, Yong-An 2018-04-01 http://ir.ihb.ac.cn/handle/342005/29573 http://ir.ihb.ac.cn/handle/342005/29574 https://doi.org/10.1016/j.fsi.2018.01.047 英语 eng ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD FISH & SHELLFISH IMMUNOLOGY http://ir.ihb.ac.cn/handle/342005/29573 http://ir.ihb.ac.cn/handle/342005/29574 doi:10.1016/j.fsi.2018.01.047 Grass carp reovirus DNA vaccine Antibodies Immunoprotection Immune response Fisheries Immunology Marine & Freshwater Biology Veterinary Sciences HEMATOPOIETIC NECROSIS VIRUS HEMORRHAGIC SEPTICEMIA VIRUS COMPLETE GENOME SEQUENCE SALMON SALMO-SALAR RAINBOW-TROUT PROTECTIVE IMMUNITY ONCORHYNCHUS-MYKISS CARBON NANOTUBES YERSINIA-RUCKERI TELEOST FISH 期刊论文 2018 ftchinacadsciihb https://doi.org/10.1016/j.fsi.2018.01.047 2019-08-30T00:03:58Z Grass carp reovirus II (GCRV II) causes severe hemorrhagic disease with high mortality in grass carp, Cyenopharyngodon idellus. DNA vaccination has been proven to be a very effective method in conferring protection against fish viruses. However, DNA vaccines for GCRV II have not yet been conducted on grass carp. In the current work, we vaccinated grass carp with a DNA vaccine consisting of the segment 6 (pC-S6; encoding VP4) or 10 (pC-S10; encoding NS38) of GCRV II and comparatively analyzed the immune responses induced by these two vaccines. The protective efficacy of pC-S6 and pC-S10, in terms of relative percentage survival (RPS), was 59.9% and 23.1% respectively. This suggests that pC-S6 and pC-S10 DNA vaccines could increase the survival rate of grass carp against GCRV, albeit with variations in immunoprotective effect. Immunological analyses indicated the following. First, post-vaccination (pv), both pC-S6 and pC-S10 up-regulated the expression of interferon (IFN-1), Mx1, IL-1 beta, and TNF-alpha. However, CD4 and CD8 alpha were up-regulated in the case of pC-S6 but not pC-S10. Second, comparing non-vaccinated and pC-S10-vaccinated fish, the T cell response related genes, such as CD4, CD8 alpha, and GATA3, were elevated in pC-S6-vaccinated fish at 48 h post-challenge (pc). Third, pC-S6 and pC-S10 induced similar patterns of specific antibody response pv. However, only anti-VP4 IgM in the sera of surviving fish infected with GCRV was significantly increased pc compared with that pre-challenge. Taken together, these results indicate that pC-S6 promotes both innate (IFN-1 and Mx1 induction) and adaptive CT cell and specific antibody response) immunity pv and that the induction of a memory state promptly primes the immune response upon later encounters with the virus, whereas pC-S10 only induces the type I IFN-related response pv and a lower inflammatory response pc. Report Salmo salar Institute of Hydrobiology, Chinese Academy of Sciences: IHB OpenIR Fish & Shellfish Immunology 75 66 73 |
institution |
Open Polar |
collection |
Institute of Hydrobiology, Chinese Academy of Sciences: IHB OpenIR |
op_collection_id |
ftchinacadsciihb |
language |
English |
topic |
Grass carp reovirus DNA vaccine Antibodies Immunoprotection Immune response Fisheries Immunology Marine & Freshwater Biology Veterinary Sciences HEMATOPOIETIC NECROSIS VIRUS HEMORRHAGIC SEPTICEMIA VIRUS COMPLETE GENOME SEQUENCE SALMON SALMO-SALAR RAINBOW-TROUT PROTECTIVE IMMUNITY ONCORHYNCHUS-MYKISS CARBON NANOTUBES YERSINIA-RUCKERI TELEOST FISH |
spellingShingle |
Grass carp reovirus DNA vaccine Antibodies Immunoprotection Immune response Fisheries Immunology Marine & Freshwater Biology Veterinary Sciences HEMATOPOIETIC NECROSIS VIRUS HEMORRHAGIC SEPTICEMIA VIRUS COMPLETE GENOME SEQUENCE SALMON SALMO-SALAR RAINBOW-TROUT PROTECTIVE IMMUNITY ONCORHYNCHUS-MYKISS CARBON NANOTUBES YERSINIA-RUCKERI TELEOST FISH Chen, Dan-Dan Yao, Yuan-Yuan Cui, Zheng-Wei Zhang, Xiang-Yang Peng, Kai-Song Guo, Xia Wang, Biao Zhou, Yuan-Yuan Li, Shun Wu, Nan Zhang, Yong-An Comparative study of the immunoprotective effect of two DNA vaccines against grass carp reovirus |
topic_facet |
Grass carp reovirus DNA vaccine Antibodies Immunoprotection Immune response Fisheries Immunology Marine & Freshwater Biology Veterinary Sciences HEMATOPOIETIC NECROSIS VIRUS HEMORRHAGIC SEPTICEMIA VIRUS COMPLETE GENOME SEQUENCE SALMON SALMO-SALAR RAINBOW-TROUT PROTECTIVE IMMUNITY ONCORHYNCHUS-MYKISS CARBON NANOTUBES YERSINIA-RUCKERI TELEOST FISH |
description |
Grass carp reovirus II (GCRV II) causes severe hemorrhagic disease with high mortality in grass carp, Cyenopharyngodon idellus. DNA vaccination has been proven to be a very effective method in conferring protection against fish viruses. However, DNA vaccines for GCRV II have not yet been conducted on grass carp. In the current work, we vaccinated grass carp with a DNA vaccine consisting of the segment 6 (pC-S6; encoding VP4) or 10 (pC-S10; encoding NS38) of GCRV II and comparatively analyzed the immune responses induced by these two vaccines. The protective efficacy of pC-S6 and pC-S10, in terms of relative percentage survival (RPS), was 59.9% and 23.1% respectively. This suggests that pC-S6 and pC-S10 DNA vaccines could increase the survival rate of grass carp against GCRV, albeit with variations in immunoprotective effect. Immunological analyses indicated the following. First, post-vaccination (pv), both pC-S6 and pC-S10 up-regulated the expression of interferon (IFN-1), Mx1, IL-1 beta, and TNF-alpha. However, CD4 and CD8 alpha were up-regulated in the case of pC-S6 but not pC-S10. Second, comparing non-vaccinated and pC-S10-vaccinated fish, the T cell response related genes, such as CD4, CD8 alpha, and GATA3, were elevated in pC-S6-vaccinated fish at 48 h post-challenge (pc). Third, pC-S6 and pC-S10 induced similar patterns of specific antibody response pv. However, only anti-VP4 IgM in the sera of surviving fish infected with GCRV was significantly increased pc compared with that pre-challenge. Taken together, these results indicate that pC-S6 promotes both innate (IFN-1 and Mx1 induction) and adaptive CT cell and specific antibody response) immunity pv and that the induction of a memory state promptly primes the immune response upon later encounters with the virus, whereas pC-S10 only induces the type I IFN-related response pv and a lower inflammatory response pc. |
format |
Report |
author |
Chen, Dan-Dan Yao, Yuan-Yuan Cui, Zheng-Wei Zhang, Xiang-Yang Peng, Kai-Song Guo, Xia Wang, Biao Zhou, Yuan-Yuan Li, Shun Wu, Nan Zhang, Yong-An |
author_facet |
Chen, Dan-Dan Yao, Yuan-Yuan Cui, Zheng-Wei Zhang, Xiang-Yang Peng, Kai-Song Guo, Xia Wang, Biao Zhou, Yuan-Yuan Li, Shun Wu, Nan Zhang, Yong-An |
author_sort |
Chen, Dan-Dan |
title |
Comparative study of the immunoprotective effect of two DNA vaccines against grass carp reovirus |
title_short |
Comparative study of the immunoprotective effect of two DNA vaccines against grass carp reovirus |
title_full |
Comparative study of the immunoprotective effect of two DNA vaccines against grass carp reovirus |
title_fullStr |
Comparative study of the immunoprotective effect of two DNA vaccines against grass carp reovirus |
title_full_unstemmed |
Comparative study of the immunoprotective effect of two DNA vaccines against grass carp reovirus |
title_sort |
comparative study of the immunoprotective effect of two dna vaccines against grass carp reovirus |
publisher |
ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD |
publishDate |
2018 |
url |
http://ir.ihb.ac.cn/handle/342005/29573 http://ir.ihb.ac.cn/handle/342005/29574 https://doi.org/10.1016/j.fsi.2018.01.047 |
genre |
Salmo salar |
genre_facet |
Salmo salar |
op_relation |
FISH & SHELLFISH IMMUNOLOGY http://ir.ihb.ac.cn/handle/342005/29573 http://ir.ihb.ac.cn/handle/342005/29574 doi:10.1016/j.fsi.2018.01.047 |
op_doi |
https://doi.org/10.1016/j.fsi.2018.01.047 |
container_title |
Fish & Shellfish Immunology |
container_volume |
75 |
container_start_page |
66 |
op_container_end_page |
73 |
_version_ |
1766182759748861952 |