The protective role of glutamine on enteropathy induced by high dose of soybean meal in turbot, Scophthalmus maximus L.
The aim of this study is to investigate the protective effect and regulatory mechanism of Gln on the enteropathy of turbot induced by soybean meal. Three isonitrogenous and isolipidic practical diets were formulated with fish meal basis (FM), soy protein replacing 40% fish meal protein (SBM) and die...
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Language: | English |
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Online Access: | http://ir.ihb.ac.cn/handle/342005/29469 https://doi.org/10.1016/j.aquaculture.2018.08.021 |
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ftchinacadsciihb:oai:ir.ihb.ac.cn:342005/29469 2023-05-15T15:32:58+02:00 The protective role of glutamine on enteropathy induced by high dose of soybean meal in turbot, Scophthalmus maximus L. Liu, Yang Chen, Zhichu Dai, Jihong Yang, Pei Hu, Haibin Ai, Qinghui Zhang, Wenbing Zhang, Yongan Zhang, Yanjiao Mai, Kangsen 2018-12-01 http://ir.ihb.ac.cn/handle/342005/29469 https://doi.org/10.1016/j.aquaculture.2018.08.021 英语 eng ELSEVIER SCIENCE BV AQUACULTURE http://ir.ihb.ac.cn/handle/342005/29469 doi:10.1016/j.aquaculture.2018.08.021 Glutamine Soybean meal Enteropathy Intestinal mucosal barrier Intestinal microbiota Turbot Fisheries Marine & Freshwater Biology NF-KAPPA-B TURBOT SCOPHTHALMUS-MAXIMUS SALMO-SALAR L ACTIVATED-RECEPTOR-GAMMA LIGHT-CHAIN KINASE BARRIER FUNCTION ATLANTIC SALMON PPAR-GAMMA EPITHELIAL BARRIER 期刊论文 2018 ftchinacadsciihb https://doi.org/10.1016/j.aquaculture.2018.08.021 2019-07-12T00:03:44Z The aim of this study is to investigate the protective effect and regulatory mechanism of Gln on the enteropathy of turbot induced by soybean meal. Three isonitrogenous and isolipidic practical diets were formulated with fish meal basis (FM), soy protein replacing 40% fish meal protein (SBM) and dietary 2% Ala-Gln in SBM (Ala-Gin) for sextuple replicates of 30 fish each tank for 12 weeks. The fish in SBM group presented distinct enteropathy including declined absorptive surface and overt infiltration of mixed leukocytes in the lamina propria. However, the turbot fed Ala-Gln diet showed the integrity of the intestinal tissue structure and higher ADC and AKP levels like fish in FM group. Compared to SBM group, the inclusion of 2% Ala-Gln in SBM-basal diet significantly elevated the expression of barrier-forming tight junction proteins claudin-4, occludin and ZO-1 and depressed the expression of pro-inflammatory cytokines TNF-alpha in the intestine. Fish fed Ala-Gln diet showed remarkable increased MUC-2 and PPAR-gamma gene expression as well as reduced expression of NF-kappa B and MLCK equivalent to the level of FM group. Additionally, Gln significantly elevated the relative abundance of Proteobacteria (Vibrio spp.) and declined Bacteroidetes (Bacteroides spp.) relative abundance. The similarity of intestinal microbial communities in Ala-Gln group was notably closer to FM group from PCoA, UPGMA and Heatmap analyses based on weighted UniFrac distance. In conclusion, dietary Gln could probably enhance intestinal barrier function to alleviate enteropathy by improving MUC-2 and PPAR-gamma expression and inhibiting NF-kappa B-MLCK signaling pathway, as well as altering microbiota. Report Atlantic salmon Salmo salar Scophthalmus maximus Turbot Institute of Hydrobiology, Chinese Academy of Sciences: IHB OpenIR Aquaculture 497 510 519 |
institution |
Open Polar |
collection |
Institute of Hydrobiology, Chinese Academy of Sciences: IHB OpenIR |
op_collection_id |
ftchinacadsciihb |
language |
English |
topic |
Glutamine Soybean meal Enteropathy Intestinal mucosal barrier Intestinal microbiota Turbot Fisheries Marine & Freshwater Biology NF-KAPPA-B TURBOT SCOPHTHALMUS-MAXIMUS SALMO-SALAR L ACTIVATED-RECEPTOR-GAMMA LIGHT-CHAIN KINASE BARRIER FUNCTION ATLANTIC SALMON PPAR-GAMMA EPITHELIAL BARRIER |
spellingShingle |
Glutamine Soybean meal Enteropathy Intestinal mucosal barrier Intestinal microbiota Turbot Fisheries Marine & Freshwater Biology NF-KAPPA-B TURBOT SCOPHTHALMUS-MAXIMUS SALMO-SALAR L ACTIVATED-RECEPTOR-GAMMA LIGHT-CHAIN KINASE BARRIER FUNCTION ATLANTIC SALMON PPAR-GAMMA EPITHELIAL BARRIER Liu, Yang Chen, Zhichu Dai, Jihong Yang, Pei Hu, Haibin Ai, Qinghui Zhang, Wenbing Zhang, Yongan Zhang, Yanjiao Mai, Kangsen The protective role of glutamine on enteropathy induced by high dose of soybean meal in turbot, Scophthalmus maximus L. |
topic_facet |
Glutamine Soybean meal Enteropathy Intestinal mucosal barrier Intestinal microbiota Turbot Fisheries Marine & Freshwater Biology NF-KAPPA-B TURBOT SCOPHTHALMUS-MAXIMUS SALMO-SALAR L ACTIVATED-RECEPTOR-GAMMA LIGHT-CHAIN KINASE BARRIER FUNCTION ATLANTIC SALMON PPAR-GAMMA EPITHELIAL BARRIER |
description |
The aim of this study is to investigate the protective effect and regulatory mechanism of Gln on the enteropathy of turbot induced by soybean meal. Three isonitrogenous and isolipidic practical diets were formulated with fish meal basis (FM), soy protein replacing 40% fish meal protein (SBM) and dietary 2% Ala-Gln in SBM (Ala-Gin) for sextuple replicates of 30 fish each tank for 12 weeks. The fish in SBM group presented distinct enteropathy including declined absorptive surface and overt infiltration of mixed leukocytes in the lamina propria. However, the turbot fed Ala-Gln diet showed the integrity of the intestinal tissue structure and higher ADC and AKP levels like fish in FM group. Compared to SBM group, the inclusion of 2% Ala-Gln in SBM-basal diet significantly elevated the expression of barrier-forming tight junction proteins claudin-4, occludin and ZO-1 and depressed the expression of pro-inflammatory cytokines TNF-alpha in the intestine. Fish fed Ala-Gln diet showed remarkable increased MUC-2 and PPAR-gamma gene expression as well as reduced expression of NF-kappa B and MLCK equivalent to the level of FM group. Additionally, Gln significantly elevated the relative abundance of Proteobacteria (Vibrio spp.) and declined Bacteroidetes (Bacteroides spp.) relative abundance. The similarity of intestinal microbial communities in Ala-Gln group was notably closer to FM group from PCoA, UPGMA and Heatmap analyses based on weighted UniFrac distance. In conclusion, dietary Gln could probably enhance intestinal barrier function to alleviate enteropathy by improving MUC-2 and PPAR-gamma expression and inhibiting NF-kappa B-MLCK signaling pathway, as well as altering microbiota. |
format |
Report |
author |
Liu, Yang Chen, Zhichu Dai, Jihong Yang, Pei Hu, Haibin Ai, Qinghui Zhang, Wenbing Zhang, Yongan Zhang, Yanjiao Mai, Kangsen |
author_facet |
Liu, Yang Chen, Zhichu Dai, Jihong Yang, Pei Hu, Haibin Ai, Qinghui Zhang, Wenbing Zhang, Yongan Zhang, Yanjiao Mai, Kangsen |
author_sort |
Liu, Yang |
title |
The protective role of glutamine on enteropathy induced by high dose of soybean meal in turbot, Scophthalmus maximus L. |
title_short |
The protective role of glutamine on enteropathy induced by high dose of soybean meal in turbot, Scophthalmus maximus L. |
title_full |
The protective role of glutamine on enteropathy induced by high dose of soybean meal in turbot, Scophthalmus maximus L. |
title_fullStr |
The protective role of glutamine on enteropathy induced by high dose of soybean meal in turbot, Scophthalmus maximus L. |
title_full_unstemmed |
The protective role of glutamine on enteropathy induced by high dose of soybean meal in turbot, Scophthalmus maximus L. |
title_sort |
protective role of glutamine on enteropathy induced by high dose of soybean meal in turbot, scophthalmus maximus l. |
publisher |
ELSEVIER SCIENCE BV |
publishDate |
2018 |
url |
http://ir.ihb.ac.cn/handle/342005/29469 https://doi.org/10.1016/j.aquaculture.2018.08.021 |
genre |
Atlantic salmon Salmo salar Scophthalmus maximus Turbot |
genre_facet |
Atlantic salmon Salmo salar Scophthalmus maximus Turbot |
op_relation |
AQUACULTURE http://ir.ihb.ac.cn/handle/342005/29469 doi:10.1016/j.aquaculture.2018.08.021 |
op_doi |
https://doi.org/10.1016/j.aquaculture.2018.08.021 |
container_title |
Aquaculture |
container_volume |
497 |
container_start_page |
510 |
op_container_end_page |
519 |
_version_ |
1766363448388616192 |