Integrative Transcriptomic and microRNAomic Profiling Reveals Immune Mechanism for the Resilience to Soybean Meal Stress in Fish Gut and Liver
In aquafeeds, fish-meal has been commonly replaced with plant protein, which often causes enteritis. Currently, foodborne enteritis has few solutions in regards to prevention or cures. The recovery mechanism from enteritis in herbivorous fish may further help understand prevention or therapy. Howeve...
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Online Access: | http://ir.ihb.ac.cn/handle/342005/29416 http://ir.ihb.ac.cn/handle/342005/29417 https://doi.org/10.3389/fphys.2018.01154 |
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ftchinacadsciihb:oai:ir.ihb.ac.cn:342005/29417 2023-05-15T15:32:50+02:00 Integrative Transcriptomic and microRNAomic Profiling Reveals Immune Mechanism for the Resilience to Soybean Meal Stress in Fish Gut and Liver Wu, Nan Wang, Biao Cui, Zheng-Wei Zhang, Xiang-Yang Cheng, Ying-Yin Xu, Xuan Li, Xian-Mei Wang, Zhao-Xi Chen, Dan-Dan Zhang, Yong-An 2018-09-10 http://ir.ihb.ac.cn/handle/342005/29416 http://ir.ihb.ac.cn/handle/342005/29417 https://doi.org/10.3389/fphys.2018.01154 英语 eng FRONTIERS MEDIA SA FRONTIERS IN PHYSIOLOGY http://ir.ihb.ac.cn/handle/342005/29416 http://ir.ihb.ac.cn/handle/342005/29417 doi:10.3389/fphys.2018.01154 soybean meal induced enteritis resilience transcriptome miRNAome gut-liver immunity grass carp Physiology SALMO-SALAR L PROLIFERATOR-ACTIVATED RECEPTOR CARP CTENOPHARYNGODON-IDELLA INFLAMMATORY-BOWEL-DISEASE ATLANTIC SALMON T-CELLS INTERLEUKIN-7 RECEPTOR ULCERATIVE-COLITIS DISTAL INTESTINE 期刊论文 2018 ftchinacadsciihb https://doi.org/10.3389/fphys.2018.01154 2019-08-30T00:03:58Z In aquafeeds, fish-meal has been commonly replaced with plant protein, which often causes enteritis. Currently, foodborne enteritis has few solutions in regards to prevention or cures. The recovery mechanism from enteritis in herbivorous fish may further help understand prevention or therapy. However, few reports could be found regarding the recovery or resilience to fish foodborne enteritis. In this study, grass carp was used as an animal model for soybean meal induced enteritis and it was found that the fish could adapt to the soybeanmeal at a moderate level of substitution. Resilience to soybeanmeal stress was found in the 40% soybean meal group for juvenile fish at growth performance, morphological and gene expression levels, after a 7-week feeding trial. Furthermore, the intestinal transcriptomic data, including transcriptome and miRNAome, was applied to demonstrate resilience mechanisms. The result of this study revealed that in juvenile grass carp after a 7-week feeding cycle with 40% soybean meal, the intestine recovered via enhancing both an immune tolerance and wound healing, the liver gradually adapted via re-balancing immune responses, such as phagosome and complement cascades. Also, many immune factors in the gut and liver were systemically revealed among stages of on-setting, remising, and recovering (or relief). In addition, miRNA regulation played a key role in switching immune states. Thus, the present data systemically demonstrated that the molecular adaptation mechanism of fish gut-liver immunity is involved in the resilience to soybean meal stress. Report Atlantic salmon Salmo salar Institute of Hydrobiology, Chinese Academy of Sciences: IHB OpenIR Frontiers in Physiology 9 |
institution |
Open Polar |
collection |
Institute of Hydrobiology, Chinese Academy of Sciences: IHB OpenIR |
op_collection_id |
ftchinacadsciihb |
language |
English |
topic |
soybean meal induced enteritis resilience transcriptome miRNAome gut-liver immunity grass carp Physiology SALMO-SALAR L PROLIFERATOR-ACTIVATED RECEPTOR CARP CTENOPHARYNGODON-IDELLA INFLAMMATORY-BOWEL-DISEASE ATLANTIC SALMON T-CELLS INTERLEUKIN-7 RECEPTOR ULCERATIVE-COLITIS DISTAL INTESTINE |
spellingShingle |
soybean meal induced enteritis resilience transcriptome miRNAome gut-liver immunity grass carp Physiology SALMO-SALAR L PROLIFERATOR-ACTIVATED RECEPTOR CARP CTENOPHARYNGODON-IDELLA INFLAMMATORY-BOWEL-DISEASE ATLANTIC SALMON T-CELLS INTERLEUKIN-7 RECEPTOR ULCERATIVE-COLITIS DISTAL INTESTINE Wu, Nan Wang, Biao Cui, Zheng-Wei Zhang, Xiang-Yang Cheng, Ying-Yin Xu, Xuan Li, Xian-Mei Wang, Zhao-Xi Chen, Dan-Dan Zhang, Yong-An Integrative Transcriptomic and microRNAomic Profiling Reveals Immune Mechanism for the Resilience to Soybean Meal Stress in Fish Gut and Liver |
topic_facet |
soybean meal induced enteritis resilience transcriptome miRNAome gut-liver immunity grass carp Physiology SALMO-SALAR L PROLIFERATOR-ACTIVATED RECEPTOR CARP CTENOPHARYNGODON-IDELLA INFLAMMATORY-BOWEL-DISEASE ATLANTIC SALMON T-CELLS INTERLEUKIN-7 RECEPTOR ULCERATIVE-COLITIS DISTAL INTESTINE |
description |
In aquafeeds, fish-meal has been commonly replaced with plant protein, which often causes enteritis. Currently, foodborne enteritis has few solutions in regards to prevention or cures. The recovery mechanism from enteritis in herbivorous fish may further help understand prevention or therapy. However, few reports could be found regarding the recovery or resilience to fish foodborne enteritis. In this study, grass carp was used as an animal model for soybean meal induced enteritis and it was found that the fish could adapt to the soybeanmeal at a moderate level of substitution. Resilience to soybeanmeal stress was found in the 40% soybean meal group for juvenile fish at growth performance, morphological and gene expression levels, after a 7-week feeding trial. Furthermore, the intestinal transcriptomic data, including transcriptome and miRNAome, was applied to demonstrate resilience mechanisms. The result of this study revealed that in juvenile grass carp after a 7-week feeding cycle with 40% soybean meal, the intestine recovered via enhancing both an immune tolerance and wound healing, the liver gradually adapted via re-balancing immune responses, such as phagosome and complement cascades. Also, many immune factors in the gut and liver were systemically revealed among stages of on-setting, remising, and recovering (or relief). In addition, miRNA regulation played a key role in switching immune states. Thus, the present data systemically demonstrated that the molecular adaptation mechanism of fish gut-liver immunity is involved in the resilience to soybean meal stress. |
format |
Report |
author |
Wu, Nan Wang, Biao Cui, Zheng-Wei Zhang, Xiang-Yang Cheng, Ying-Yin Xu, Xuan Li, Xian-Mei Wang, Zhao-Xi Chen, Dan-Dan Zhang, Yong-An |
author_facet |
Wu, Nan Wang, Biao Cui, Zheng-Wei Zhang, Xiang-Yang Cheng, Ying-Yin Xu, Xuan Li, Xian-Mei Wang, Zhao-Xi Chen, Dan-Dan Zhang, Yong-An |
author_sort |
Wu, Nan |
title |
Integrative Transcriptomic and microRNAomic Profiling Reveals Immune Mechanism for the Resilience to Soybean Meal Stress in Fish Gut and Liver |
title_short |
Integrative Transcriptomic and microRNAomic Profiling Reveals Immune Mechanism for the Resilience to Soybean Meal Stress in Fish Gut and Liver |
title_full |
Integrative Transcriptomic and microRNAomic Profiling Reveals Immune Mechanism for the Resilience to Soybean Meal Stress in Fish Gut and Liver |
title_fullStr |
Integrative Transcriptomic and microRNAomic Profiling Reveals Immune Mechanism for the Resilience to Soybean Meal Stress in Fish Gut and Liver |
title_full_unstemmed |
Integrative Transcriptomic and microRNAomic Profiling Reveals Immune Mechanism for the Resilience to Soybean Meal Stress in Fish Gut and Liver |
title_sort |
integrative transcriptomic and micrornaomic profiling reveals immune mechanism for the resilience to soybean meal stress in fish gut and liver |
publisher |
FRONTIERS MEDIA SA |
publishDate |
2018 |
url |
http://ir.ihb.ac.cn/handle/342005/29416 http://ir.ihb.ac.cn/handle/342005/29417 https://doi.org/10.3389/fphys.2018.01154 |
genre |
Atlantic salmon Salmo salar |
genre_facet |
Atlantic salmon Salmo salar |
op_relation |
FRONTIERS IN PHYSIOLOGY http://ir.ihb.ac.cn/handle/342005/29416 http://ir.ihb.ac.cn/handle/342005/29417 doi:10.3389/fphys.2018.01154 |
op_doi |
https://doi.org/10.3389/fphys.2018.01154 |
container_title |
Frontiers in Physiology |
container_volume |
9 |
_version_ |
1766363315522502656 |