Dietary citric acid supplementation alleviates soybean meal-induced intestinal oxidative damage and micro-ecological imbalance in juvenile turbot, Scophthalmus maximus L
A 12-week feeding trial was conducted to investigate the potential protective effects of citric acid against soybean meal-induced intestinal oxidative damage and micro-ecological imbalance in juvenile turbot (S. maximus L.). Four isonitrogenous and isolipidic experimental diets, that is fish meal-ba...
Published in: | Aquaculture Research |
---|---|
Main Authors: | , , , , , , , , , |
Format: | Report |
Language: | English |
Published: |
WILEY
2018
|
Subjects: | |
Online Access: | http://ir.ihb.ac.cn/handle/342005/29027 http://ir.ihb.ac.cn/handle/342005/29028 https://doi.org/10.1111/are.13847 |
id |
ftchinacadsciihb:oai:ir.ihb.ac.cn:342005/29028 |
---|---|
record_format |
openpolar |
spelling |
ftchinacadsciihb:oai:ir.ihb.ac.cn:342005/29028 2023-05-15T18:09:58+02:00 Dietary citric acid supplementation alleviates soybean meal-induced intestinal oxidative damage and micro-ecological imbalance in juvenile turbot, Scophthalmus maximus L Chen, Zhichu Zhao, Sifan Liu, Yang Yang, Pei Ai, Qinghui Zhang, Wenbing Xu, Weiqi Zhang, Yongan Zhang, Yanjiao Mai, Kangsen 2018-12-01 http://ir.ihb.ac.cn/handle/342005/29027 http://ir.ihb.ac.cn/handle/342005/29028 https://doi.org/10.1111/are.13847 英语 eng WILEY AQUACULTURE RESEARCH http://ir.ihb.ac.cn/handle/342005/29027 http://ir.ihb.ac.cn/handle/342005/29028 doi:10.1111/are.13847 citric acid intestinal barrier function oxidative stress soybean meal turbot Fisheries DIGESTIVE ENZYME-ACTIVITIES SHRIMP LITOPENAEUS-VANNAMEI AIRWAY EPITHELIAL-CELLS TILAPIA OREOCHROMIS SP CHAIN FATTY-ACIDS SALMO-SALAR L GROWTH-PERFORMANCE ORGANIC-ACIDS GUT MICROBIOTA HOST-DEFENSE 期刊论文 2018 ftchinacadsciihb https://doi.org/10.1111/are.13847 2019-08-30T00:03:55Z A 12-week feeding trial was conducted to investigate the potential protective effects of citric acid against soybean meal-induced intestinal oxidative damage and micro-ecological imbalance in juvenile turbot (S. maximus L.). Four isonitrogenous and isolipidic experimental diets, that is fish meal-based diet (FM), FM with 40% fish meal protein replaced with soybean meal protein diet (SBM), supplemented with 1.5% citric acid (1.5% CA) or 3% citric acid (3% CA). Results showed that both citric acid diets significantly enhanced the total antioxidative capacity and the gene expression of superoxide dismutase, glutathione peroxidase and heme oxygenase 1, while also decreasing the malondialdehyde content in the distal intestine. Compared to diet SBM, the genes expression of p53, protein kinase C and Caspase-3 were remarkably declined by dietary citric acid supplementation, while the genes expression of proliferating cell nuclear antigen and mucins showed an opposite trend. The structural integrity of the distal intestine in fish fed citric acid was showed in the histological results. Sequencing of bacterial 16s rRNA V4 region showed that the profile of intestinal bacteria was altered by dietary citric acid supplementation, which was supported by the diet-cluster of PCoA and phylogenetic tree. MetaStat analysis indicated that dietary citric acid dramatically reduced the relative abundance of the Vibrio genus. In conclusion, dietary citric acid mitigated soybean meal-induced intestinal oxidative damage, beneficially alleviated the micro-ecological imbalance and specifically reduced the relative abundance of the Vibrio genus in the distal intestine of juvenile turbot. Report Salmo salar Scophthalmus maximus Turbot Institute of Hydrobiology, Chinese Academy of Sciences: IHB OpenIR Aquaculture Research 49 12 3804 3816 |
institution |
Open Polar |
collection |
Institute of Hydrobiology, Chinese Academy of Sciences: IHB OpenIR |
op_collection_id |
ftchinacadsciihb |
language |
English |
topic |
citric acid intestinal barrier function oxidative stress soybean meal turbot Fisheries DIGESTIVE ENZYME-ACTIVITIES SHRIMP LITOPENAEUS-VANNAMEI AIRWAY EPITHELIAL-CELLS TILAPIA OREOCHROMIS SP CHAIN FATTY-ACIDS SALMO-SALAR L GROWTH-PERFORMANCE ORGANIC-ACIDS GUT MICROBIOTA HOST-DEFENSE |
spellingShingle |
citric acid intestinal barrier function oxidative stress soybean meal turbot Fisheries DIGESTIVE ENZYME-ACTIVITIES SHRIMP LITOPENAEUS-VANNAMEI AIRWAY EPITHELIAL-CELLS TILAPIA OREOCHROMIS SP CHAIN FATTY-ACIDS SALMO-SALAR L GROWTH-PERFORMANCE ORGANIC-ACIDS GUT MICROBIOTA HOST-DEFENSE Chen, Zhichu Zhao, Sifan Liu, Yang Yang, Pei Ai, Qinghui Zhang, Wenbing Xu, Weiqi Zhang, Yongan Zhang, Yanjiao Mai, Kangsen Dietary citric acid supplementation alleviates soybean meal-induced intestinal oxidative damage and micro-ecological imbalance in juvenile turbot, Scophthalmus maximus L |
topic_facet |
citric acid intestinal barrier function oxidative stress soybean meal turbot Fisheries DIGESTIVE ENZYME-ACTIVITIES SHRIMP LITOPENAEUS-VANNAMEI AIRWAY EPITHELIAL-CELLS TILAPIA OREOCHROMIS SP CHAIN FATTY-ACIDS SALMO-SALAR L GROWTH-PERFORMANCE ORGANIC-ACIDS GUT MICROBIOTA HOST-DEFENSE |
description |
A 12-week feeding trial was conducted to investigate the potential protective effects of citric acid against soybean meal-induced intestinal oxidative damage and micro-ecological imbalance in juvenile turbot (S. maximus L.). Four isonitrogenous and isolipidic experimental diets, that is fish meal-based diet (FM), FM with 40% fish meal protein replaced with soybean meal protein diet (SBM), supplemented with 1.5% citric acid (1.5% CA) or 3% citric acid (3% CA). Results showed that both citric acid diets significantly enhanced the total antioxidative capacity and the gene expression of superoxide dismutase, glutathione peroxidase and heme oxygenase 1, while also decreasing the malondialdehyde content in the distal intestine. Compared to diet SBM, the genes expression of p53, protein kinase C and Caspase-3 were remarkably declined by dietary citric acid supplementation, while the genes expression of proliferating cell nuclear antigen and mucins showed an opposite trend. The structural integrity of the distal intestine in fish fed citric acid was showed in the histological results. Sequencing of bacterial 16s rRNA V4 region showed that the profile of intestinal bacteria was altered by dietary citric acid supplementation, which was supported by the diet-cluster of PCoA and phylogenetic tree. MetaStat analysis indicated that dietary citric acid dramatically reduced the relative abundance of the Vibrio genus. In conclusion, dietary citric acid mitigated soybean meal-induced intestinal oxidative damage, beneficially alleviated the micro-ecological imbalance and specifically reduced the relative abundance of the Vibrio genus in the distal intestine of juvenile turbot. |
format |
Report |
author |
Chen, Zhichu Zhao, Sifan Liu, Yang Yang, Pei Ai, Qinghui Zhang, Wenbing Xu, Weiqi Zhang, Yongan Zhang, Yanjiao Mai, Kangsen |
author_facet |
Chen, Zhichu Zhao, Sifan Liu, Yang Yang, Pei Ai, Qinghui Zhang, Wenbing Xu, Weiqi Zhang, Yongan Zhang, Yanjiao Mai, Kangsen |
author_sort |
Chen, Zhichu |
title |
Dietary citric acid supplementation alleviates soybean meal-induced intestinal oxidative damage and micro-ecological imbalance in juvenile turbot, Scophthalmus maximus L |
title_short |
Dietary citric acid supplementation alleviates soybean meal-induced intestinal oxidative damage and micro-ecological imbalance in juvenile turbot, Scophthalmus maximus L |
title_full |
Dietary citric acid supplementation alleviates soybean meal-induced intestinal oxidative damage and micro-ecological imbalance in juvenile turbot, Scophthalmus maximus L |
title_fullStr |
Dietary citric acid supplementation alleviates soybean meal-induced intestinal oxidative damage and micro-ecological imbalance in juvenile turbot, Scophthalmus maximus L |
title_full_unstemmed |
Dietary citric acid supplementation alleviates soybean meal-induced intestinal oxidative damage and micro-ecological imbalance in juvenile turbot, Scophthalmus maximus L |
title_sort |
dietary citric acid supplementation alleviates soybean meal-induced intestinal oxidative damage and micro-ecological imbalance in juvenile turbot, scophthalmus maximus l |
publisher |
WILEY |
publishDate |
2018 |
url |
http://ir.ihb.ac.cn/handle/342005/29027 http://ir.ihb.ac.cn/handle/342005/29028 https://doi.org/10.1111/are.13847 |
genre |
Salmo salar Scophthalmus maximus Turbot |
genre_facet |
Salmo salar Scophthalmus maximus Turbot |
op_relation |
AQUACULTURE RESEARCH http://ir.ihb.ac.cn/handle/342005/29027 http://ir.ihb.ac.cn/handle/342005/29028 doi:10.1111/are.13847 |
op_doi |
https://doi.org/10.1111/are.13847 |
container_title |
Aquaculture Research |
container_volume |
49 |
container_issue |
12 |
container_start_page |
3804 |
op_container_end_page |
3816 |
_version_ |
1766182687434866688 |