Tissue distribution and depuration of the extracted hepatotoxic cyanotoxin microcystins in crucian carp (Carassius carassius) intraperitoneally injected at a sublethal dose
An acute toxicity experiment was conducted by intraperitoneal injection with a sublethal dose of extracted microcystins (MCs), 50 mu g MC-LR (where L = leucine and R = arginine) equivalent/kg body weight (BW), to examine tissue distribution and depuration of MCs in crucian carp (Carassius carassius)...
Published in: | The Scientific World JOURNAL |
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Main Authors: | , , , , , , |
Format: | Article in Journal/Newspaper |
Language: | English |
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2008
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Online Access: | http://ir.ihb.ac.cn/handle/152342/8046 https://doi.org/10.1100/tsw.2008.101 |
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ftchinacadsciihb:oai:ir.ihb.ac.cn:152342/8046 |
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record_format |
openpolar |
institution |
Open Polar |
collection |
Institute of Hydrobiology, Chinese Academy of Sciences: IHB OpenIR |
op_collection_id |
ftchinacadsciihb |
language |
English |
topic |
Microcystins Intraperitoneal Injection Crucian Carp Tissue Distribution Depuration Environmental Sciences Multidisciplinary Sciences Science & Technology Life Sciences & Biomedicine Environmental Sciences & Ecology Science & Technology - Other Topics PHYTOPLANKTIVOROUS SILVER CARP TROUT ONCORHYNCHUS-MYKISS BIOCHEMICAL-CHARACTERIZATION ATLANTIC SALMON CYPRINUS-CARPIO CELL-EXTRACTS BIGHEAD CARP BROWN TROUT IN-VIVO LR |
spellingShingle |
Microcystins Intraperitoneal Injection Crucian Carp Tissue Distribution Depuration Environmental Sciences Multidisciplinary Sciences Science & Technology Life Sciences & Biomedicine Environmental Sciences & Ecology Science & Technology - Other Topics PHYTOPLANKTIVOROUS SILVER CARP TROUT ONCORHYNCHUS-MYKISS BIOCHEMICAL-CHARACTERIZATION ATLANTIC SALMON CYPRINUS-CARPIO CELL-EXTRACTS BIGHEAD CARP BROWN TROUT IN-VIVO LR Lei, Hehua Xie, Ping Chen, Jun Liang, Gaodao Yu, Ting Jiang, Yan Xie, P, Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol China, Donghu Expt Stn Lake Ecosyst, Wuhan 430072, Peoples R China Tissue distribution and depuration of the extracted hepatotoxic cyanotoxin microcystins in crucian carp (Carassius carassius) intraperitoneally injected at a sublethal dose |
topic_facet |
Microcystins Intraperitoneal Injection Crucian Carp Tissue Distribution Depuration Environmental Sciences Multidisciplinary Sciences Science & Technology Life Sciences & Biomedicine Environmental Sciences & Ecology Science & Technology - Other Topics PHYTOPLANKTIVOROUS SILVER CARP TROUT ONCORHYNCHUS-MYKISS BIOCHEMICAL-CHARACTERIZATION ATLANTIC SALMON CYPRINUS-CARPIO CELL-EXTRACTS BIGHEAD CARP BROWN TROUT IN-VIVO LR |
description |
An acute toxicity experiment was conducted by intraperitoneal injection with a sublethal dose of extracted microcystins (MCs), 50 mu g MC-LR (where L = leucine and R = arginine) equivalent/kg body weight (BW), to examine tissue distribution and depuration of MCs in crucian carp (Carassius carassius). Liver to body weight ratio increased at 3, 12, 24, and 48 h postinjection compared with that at 0 h (p < 0.05). MC concentrations in various tissues and aquaria water were analyzed at 1, 3, 12, 24, 48, and 168 h postinjection using liquid chromatography coupled with mass spectrometry (LC-MS). The highest concentration of MCs (MC-RR + MC-LR) was found in blood, 2 -270 ng/g dry weight (DW), followed by heart (3 -100 ng/g DW) and kidney (13 -88 ng/g DW). MC levels were relatively low in liver, gonad, intestine, spleen, and brain. MC contents in gills, gallbladder, and muscle were below the limit of detection. Significant negative correlation was present between MC-RR concentration in blood and that in kidney, confirming that blood was important in the transportation of MC-RR to kidney for excretion. Rapid accumulation and slow degradation of MCs were observed in gonad, liver, intestine, spleen, and brain. Only 0.07% of injected MCs were detected in liver. The recovery of MCs in liver of crucian carp seemed to be dose dependent. An acute toxicity experiment was conducted by intraperitoneal injection with a sublethal dose of extracted microcystins (MCs), 50 mu g MC-LR (where L = leucine and R = arginine) equivalent/kg body weight (BW), to examine tissue distribution and depuration of MCs in crucian carp (Carassius carassius). Liver to body weight ratio increased at 3, 12, 24, and 48 h postinjection compared with that at 0 h (p < 0.05). MC concentrations in various tissues and aquaria water were analyzed at 1, 3, 12, 24, 48, and 168 h postinjection using liquid chromatography coupled with mass spectrometry (LC-MS). The highest concentration of MCs (MC-RR + MC-LR) was found in blood, 2 -270 ng/g dry weight (DW), followed by heart (3 -100 ng/g DW) and kidney (13 -88 ng/g DW). MC levels were relatively low in liver, gonad, intestine, spleen, and brain. MC contents in gills, gallbladder, and muscle were below the limit of detection. Significant negative correlation was present between MC-RR concentration in blood and that in kidney, confirming that blood was important in the transportation of MC-RR to kidney for excretion. Rapid accumulation and slow degradation of MCs were observed in gonad, liver, intestine, spleen, and brain. Only 0.07% of injected MCs were detected in liver. The recovery of MCs in liver of crucian carp seemed to be dose dependent. |
format |
Article in Journal/Newspaper |
author |
Lei, Hehua Xie, Ping Chen, Jun Liang, Gaodao Yu, Ting Jiang, Yan Xie, P, Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol China, Donghu Expt Stn Lake Ecosyst, Wuhan 430072, Peoples R China |
author_facet |
Lei, Hehua Xie, Ping Chen, Jun Liang, Gaodao Yu, Ting Jiang, Yan Xie, P, Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol China, Donghu Expt Stn Lake Ecosyst, Wuhan 430072, Peoples R China |
author_sort |
Lei, Hehua |
title |
Tissue distribution and depuration of the extracted hepatotoxic cyanotoxin microcystins in crucian carp (Carassius carassius) intraperitoneally injected at a sublethal dose |
title_short |
Tissue distribution and depuration of the extracted hepatotoxic cyanotoxin microcystins in crucian carp (Carassius carassius) intraperitoneally injected at a sublethal dose |
title_full |
Tissue distribution and depuration of the extracted hepatotoxic cyanotoxin microcystins in crucian carp (Carassius carassius) intraperitoneally injected at a sublethal dose |
title_fullStr |
Tissue distribution and depuration of the extracted hepatotoxic cyanotoxin microcystins in crucian carp (Carassius carassius) intraperitoneally injected at a sublethal dose |
title_full_unstemmed |
Tissue distribution and depuration of the extracted hepatotoxic cyanotoxin microcystins in crucian carp (Carassius carassius) intraperitoneally injected at a sublethal dose |
title_sort |
tissue distribution and depuration of the extracted hepatotoxic cyanotoxin microcystins in crucian carp (carassius carassius) intraperitoneally injected at a sublethal dose |
publishDate |
2008 |
url |
http://ir.ihb.ac.cn/handle/152342/8046 https://doi.org/10.1100/tsw.2008.101 |
genre |
Atlantic salmon |
genre_facet |
Atlantic salmon |
op_relation |
THESCIENTIFICWORLDJOURNAL Lei, Hehua; Xie, Ping; Chen, Jun; Liang, Gaodao; Yu, Ting; Jiang, Yan.Tissue distribution and depuration of the extracted hepatotoxic cyanotoxin microcystins in crucian carp (Carassius carassius) intraperitoneally injected at a sublethal dose,THESCIENTIFICWORLDJOURNAL,2008,8():713-719 http://ir.ihb.ac.cn/handle/152342/8046 doi:10.1100/tsw.2008.101 |
op_doi |
https://doi.org/10.1100/tsw.2008.101 |
container_title |
The Scientific World JOURNAL |
container_volume |
8 |
container_start_page |
713 |
op_container_end_page |
719 |
_version_ |
1766363614045798400 |
spelling |
ftchinacadsciihb:oai:ir.ihb.ac.cn:152342/8046 2023-05-15T15:33:09+02:00 Tissue distribution and depuration of the extracted hepatotoxic cyanotoxin microcystins in crucian carp (Carassius carassius) intraperitoneally injected at a sublethal dose Lei, Hehua Xie, Ping Chen, Jun Liang, Gaodao Yu, Ting Jiang, Yan Xie, P, Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol China, Donghu Expt Stn Lake Ecosyst, Wuhan 430072, Peoples R China 2008 http://ir.ihb.ac.cn/handle/152342/8046 https://doi.org/10.1100/tsw.2008.101 英语 eng THESCIENTIFICWORLDJOURNAL Lei, Hehua; Xie, Ping; Chen, Jun; Liang, Gaodao; Yu, Ting; Jiang, Yan.Tissue distribution and depuration of the extracted hepatotoxic cyanotoxin microcystins in crucian carp (Carassius carassius) intraperitoneally injected at a sublethal dose,THESCIENTIFICWORLDJOURNAL,2008,8():713-719 http://ir.ihb.ac.cn/handle/152342/8046 doi:10.1100/tsw.2008.101 Microcystins Intraperitoneal Injection Crucian Carp Tissue Distribution Depuration Environmental Sciences Multidisciplinary Sciences Science & Technology Life Sciences & Biomedicine Environmental Sciences & Ecology Science & Technology - Other Topics PHYTOPLANKTIVOROUS SILVER CARP TROUT ONCORHYNCHUS-MYKISS BIOCHEMICAL-CHARACTERIZATION ATLANTIC SALMON CYPRINUS-CARPIO CELL-EXTRACTS BIGHEAD CARP BROWN TROUT IN-VIVO LR Article 期刊论文 2008 ftchinacadsciihb https://doi.org/10.1100/tsw.2008.101 2019-07-01T11:26:46Z An acute toxicity experiment was conducted by intraperitoneal injection with a sublethal dose of extracted microcystins (MCs), 50 mu g MC-LR (where L = leucine and R = arginine) equivalent/kg body weight (BW), to examine tissue distribution and depuration of MCs in crucian carp (Carassius carassius). Liver to body weight ratio increased at 3, 12, 24, and 48 h postinjection compared with that at 0 h (p < 0.05). MC concentrations in various tissues and aquaria water were analyzed at 1, 3, 12, 24, 48, and 168 h postinjection using liquid chromatography coupled with mass spectrometry (LC-MS). The highest concentration of MCs (MC-RR + MC-LR) was found in blood, 2 -270 ng/g dry weight (DW), followed by heart (3 -100 ng/g DW) and kidney (13 -88 ng/g DW). MC levels were relatively low in liver, gonad, intestine, spleen, and brain. MC contents in gills, gallbladder, and muscle were below the limit of detection. Significant negative correlation was present between MC-RR concentration in blood and that in kidney, confirming that blood was important in the transportation of MC-RR to kidney for excretion. Rapid accumulation and slow degradation of MCs were observed in gonad, liver, intestine, spleen, and brain. Only 0.07% of injected MCs were detected in liver. The recovery of MCs in liver of crucian carp seemed to be dose dependent. An acute toxicity experiment was conducted by intraperitoneal injection with a sublethal dose of extracted microcystins (MCs), 50 mu g MC-LR (where L = leucine and R = arginine) equivalent/kg body weight (BW), to examine tissue distribution and depuration of MCs in crucian carp (Carassius carassius). Liver to body weight ratio increased at 3, 12, 24, and 48 h postinjection compared with that at 0 h (p < 0.05). MC concentrations in various tissues and aquaria water were analyzed at 1, 3, 12, 24, 48, and 168 h postinjection using liquid chromatography coupled with mass spectrometry (LC-MS). The highest concentration of MCs (MC-RR + MC-LR) was found in blood, 2 -270 ng/g dry weight (DW), followed by heart (3 -100 ng/g DW) and kidney (13 -88 ng/g DW). MC levels were relatively low in liver, gonad, intestine, spleen, and brain. MC contents in gills, gallbladder, and muscle were below the limit of detection. Significant negative correlation was present between MC-RR concentration in blood and that in kidney, confirming that blood was important in the transportation of MC-RR to kidney for excretion. Rapid accumulation and slow degradation of MCs were observed in gonad, liver, intestine, spleen, and brain. Only 0.07% of injected MCs were detected in liver. The recovery of MCs in liver of crucian carp seemed to be dose dependent. Article in Journal/Newspaper Atlantic salmon Institute of Hydrobiology, Chinese Academy of Sciences: IHB OpenIR The Scientific World JOURNAL 8 713 719 |