Distribution of IgM, IgD and IgZ in mandarin fish, Siniperca chuatsi lymphoid tissues and their transcriptional changes after Flavobacterium columnare stimulation
The gene sequences of three different immunoglobulin (Ig) heavy chains, namely IgM, IgD and IgZ, were cloned from mandarin fish (Siniperca chuatsi) recently. In this study the distribution of these three kinds of Ig-producing cells in lymphoid-related tissues as head kidney, spleen, gill and intesti...
Published in: | Aquaculture |
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Main Authors: | , , , , , |
Format: | Article in Journal/Newspaper |
Language: | English |
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2009
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Online Access: | http://ir.ihb.ac.cn/handle/152342/7842 https://doi.org/10.1016/j.aquaculture.2008.11.023 |
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ftchinacadsciihb:oai:ir.ihb.ac.cn:152342/7842 |
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openpolar |
institution |
Open Polar |
collection |
Institute of Hydrobiology, Chinese Academy of Sciences: IHB OpenIR |
op_collection_id |
ftchinacadsciihb |
language |
English |
topic |
Mandarin Fish Siniperca Chuatsi In Situ Hybridization Quantitive Real-time Pcr Immunoglobulin Lymphoid Tissues Fisheries Marine & Freshwater Biology Science & Technology Life Sciences & Biomedicine IMMUNOGLOBULIN HEAVY-CHAIN BASS DICENTRARCHUS-LABRAX ANARHICHAS-MINOR OLAFSEN TROUT ONCORHYNCHUS-MYKISS IN-SITU HYBRIDIZATION CYPRINUS-CARPIO L RAINBOW-TROUT ATLANTIC SALMON CHANNEL CATFISH CONSTANT-REGION |
spellingShingle |
Mandarin Fish Siniperca Chuatsi In Situ Hybridization Quantitive Real-time Pcr Immunoglobulin Lymphoid Tissues Fisheries Marine & Freshwater Biology Science & Technology Life Sciences & Biomedicine IMMUNOGLOBULIN HEAVY-CHAIN BASS DICENTRARCHUS-LABRAX ANARHICHAS-MINOR OLAFSEN TROUT ONCORHYNCHUS-MYKISS IN-SITU HYBRIDIZATION CYPRINUS-CARPIO L RAINBOW-TROUT ATLANTIC SALMON CHANNEL CATFISH CONSTANT-REGION Tian, Jingyun Sun, Baojian Luo, Yanping Zhang, Yongan Nie, Pin Nie, P, Chinese Acad Sci, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Hubei Province, Peoples R China Distribution of IgM, IgD and IgZ in mandarin fish, Siniperca chuatsi lymphoid tissues and their transcriptional changes after Flavobacterium columnare stimulation |
topic_facet |
Mandarin Fish Siniperca Chuatsi In Situ Hybridization Quantitive Real-time Pcr Immunoglobulin Lymphoid Tissues Fisheries Marine & Freshwater Biology Science & Technology Life Sciences & Biomedicine IMMUNOGLOBULIN HEAVY-CHAIN BASS DICENTRARCHUS-LABRAX ANARHICHAS-MINOR OLAFSEN TROUT ONCORHYNCHUS-MYKISS IN-SITU HYBRIDIZATION CYPRINUS-CARPIO L RAINBOW-TROUT ATLANTIC SALMON CHANNEL CATFISH CONSTANT-REGION |
description |
The gene sequences of three different immunoglobulin (Ig) heavy chains, namely IgM, IgD and IgZ, were cloned from mandarin fish (Siniperca chuatsi) recently. In this study the distribution of these three kinds of Ig-producing cells in lymphoid-related tissues as head kidney, spleen, gill and intestine were investigated by using in situ hybridization, and their transcriptional changes were also analyzed by quantitative real-time PCR during 8 weeks after immunization. IgM-producing cells could be detected obviously and abundantly in all the tissues examined. A few numbers of IgD and IgZ positive cells were both detected in head kidney and spleen. IgZ positive cells could be detected in gill moderately while IgD showed negative results, otherwise no IgD or IgZ positive cells could be detected in intestine. After stimulated with bacterial pathogen Flavobacterium columnare G(4), the transcripts of these three Ig genes exhibited quite different kinetics. Significantly increased transcription of IgM gene was observed in almost all the tissues examined especially in boosted group. In contrast with IgM, seldom strong increase was examined for IgD and IgZ genes. For IgD, it seemed that the first injection could stimulate the immune response easier, since in almost all the tissues significant increase was detected at 1 or 2 weeks after injection. For IgZ, boosted injection could not enlarge the up-regulation of gene expression of first injection. This is the first case to report the transcriptional kinetics of three Ig genes in teleost after bacterin immunization. (C) 2008 Elsevier B.V. All rights reserved. The gene sequences of three different immunoglobulin (Ig) heavy chains, namely IgM, IgD and IgZ, were cloned from mandarin fish (Siniperca chuatsi) recently. In this study the distribution of these three kinds of Ig-producing cells in lymphoid-related tissues as head kidney, spleen, gill and intestine were investigated by using in situ hybridization, and their transcriptional changes were also analyzed by quantitative real-time PCR during 8 weeks after immunization. IgM-producing cells could be detected obviously and abundantly in all the tissues examined. A few numbers of IgD and IgZ positive cells were both detected in head kidney and spleen. IgZ positive cells could be detected in gill moderately while IgD showed negative results, otherwise no IgD or IgZ positive cells could be detected in intestine. After stimulated with bacterial pathogen Flavobacterium columnare G(4), the transcripts of these three Ig genes exhibited quite different kinetics. Significantly increased transcription of IgM gene was observed in almost all the tissues examined especially in boosted group. In contrast with IgM, seldom strong increase was examined for IgD and IgZ genes. For IgD, it seemed that the first injection could stimulate the immune response easier, since in almost all the tissues significant increase was detected at 1 or 2 weeks after injection. For IgZ, boosted injection could not enlarge the up-regulation of gene expression of first injection. This is the first case to report the transcriptional kinetics of three Ig genes in teleost after bacterin immunization. (C) 2008 Elsevier B.V. All rights reserved. |
format |
Article in Journal/Newspaper |
author |
Tian, Jingyun Sun, Baojian Luo, Yanping Zhang, Yongan Nie, Pin Nie, P, Chinese Acad Sci, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Hubei Province, Peoples R China |
author_facet |
Tian, Jingyun Sun, Baojian Luo, Yanping Zhang, Yongan Nie, Pin Nie, P, Chinese Acad Sci, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Hubei Province, Peoples R China |
author_sort |
Tian, Jingyun |
title |
Distribution of IgM, IgD and IgZ in mandarin fish, Siniperca chuatsi lymphoid tissues and their transcriptional changes after Flavobacterium columnare stimulation |
title_short |
Distribution of IgM, IgD and IgZ in mandarin fish, Siniperca chuatsi lymphoid tissues and their transcriptional changes after Flavobacterium columnare stimulation |
title_full |
Distribution of IgM, IgD and IgZ in mandarin fish, Siniperca chuatsi lymphoid tissues and their transcriptional changes after Flavobacterium columnare stimulation |
title_fullStr |
Distribution of IgM, IgD and IgZ in mandarin fish, Siniperca chuatsi lymphoid tissues and their transcriptional changes after Flavobacterium columnare stimulation |
title_full_unstemmed |
Distribution of IgM, IgD and IgZ in mandarin fish, Siniperca chuatsi lymphoid tissues and their transcriptional changes after Flavobacterium columnare stimulation |
title_sort |
distribution of igm, igd and igz in mandarin fish, siniperca chuatsi lymphoid tissues and their transcriptional changes after flavobacterium columnare stimulation |
publishDate |
2009 |
url |
http://ir.ihb.ac.cn/handle/152342/7842 https://doi.org/10.1016/j.aquaculture.2008.11.023 |
genre |
Atlantic salmon |
genre_facet |
Atlantic salmon |
op_relation |
AQUACULTURE Tian, Jingyun; Sun, Baojian; Luo, Yanping; Zhang, Yongan; Nie, Pin.Distribution of IgM, IgD and IgZ in mandarin fish, Siniperca chuatsi lymphoid tissues and their transcriptional changes after Flavobacterium columnare stimulation,AQUACULTURE,2009,288(1-2):14-21 http://ir.ihb.ac.cn/handle/152342/7842 doi:10.1016/j.aquaculture.2008.11.023 |
op_doi |
https://doi.org/10.1016/j.aquaculture.2008.11.023 |
container_title |
Aquaculture |
container_volume |
288 |
container_issue |
1-2 |
container_start_page |
14 |
op_container_end_page |
21 |
_version_ |
1766363626601447424 |
spelling |
ftchinacadsciihb:oai:ir.ihb.ac.cn:152342/7842 2023-05-15T15:33:09+02:00 Distribution of IgM, IgD and IgZ in mandarin fish, Siniperca chuatsi lymphoid tissues and their transcriptional changes after Flavobacterium columnare stimulation Tian, Jingyun Sun, Baojian Luo, Yanping Zhang, Yongan Nie, Pin Nie, P, Chinese Acad Sci, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Hubei Province, Peoples R China 2009-03-02 http://ir.ihb.ac.cn/handle/152342/7842 https://doi.org/10.1016/j.aquaculture.2008.11.023 英语 eng AQUACULTURE Tian, Jingyun; Sun, Baojian; Luo, Yanping; Zhang, Yongan; Nie, Pin.Distribution of IgM, IgD and IgZ in mandarin fish, Siniperca chuatsi lymphoid tissues and their transcriptional changes after Flavobacterium columnare stimulation,AQUACULTURE,2009,288(1-2):14-21 http://ir.ihb.ac.cn/handle/152342/7842 doi:10.1016/j.aquaculture.2008.11.023 Mandarin Fish Siniperca Chuatsi In Situ Hybridization Quantitive Real-time Pcr Immunoglobulin Lymphoid Tissues Fisheries Marine & Freshwater Biology Science & Technology Life Sciences & Biomedicine IMMUNOGLOBULIN HEAVY-CHAIN BASS DICENTRARCHUS-LABRAX ANARHICHAS-MINOR OLAFSEN TROUT ONCORHYNCHUS-MYKISS IN-SITU HYBRIDIZATION CYPRINUS-CARPIO L RAINBOW-TROUT ATLANTIC SALMON CHANNEL CATFISH CONSTANT-REGION Article 期刊论文 2009 ftchinacadsciihb https://doi.org/10.1016/j.aquaculture.2008.11.023 2019-07-01T11:26:42Z The gene sequences of three different immunoglobulin (Ig) heavy chains, namely IgM, IgD and IgZ, were cloned from mandarin fish (Siniperca chuatsi) recently. In this study the distribution of these three kinds of Ig-producing cells in lymphoid-related tissues as head kidney, spleen, gill and intestine were investigated by using in situ hybridization, and their transcriptional changes were also analyzed by quantitative real-time PCR during 8 weeks after immunization. IgM-producing cells could be detected obviously and abundantly in all the tissues examined. A few numbers of IgD and IgZ positive cells were both detected in head kidney and spleen. IgZ positive cells could be detected in gill moderately while IgD showed negative results, otherwise no IgD or IgZ positive cells could be detected in intestine. After stimulated with bacterial pathogen Flavobacterium columnare G(4), the transcripts of these three Ig genes exhibited quite different kinetics. Significantly increased transcription of IgM gene was observed in almost all the tissues examined especially in boosted group. In contrast with IgM, seldom strong increase was examined for IgD and IgZ genes. For IgD, it seemed that the first injection could stimulate the immune response easier, since in almost all the tissues significant increase was detected at 1 or 2 weeks after injection. For IgZ, boosted injection could not enlarge the up-regulation of gene expression of first injection. This is the first case to report the transcriptional kinetics of three Ig genes in teleost after bacterin immunization. (C) 2008 Elsevier B.V. All rights reserved. The gene sequences of three different immunoglobulin (Ig) heavy chains, namely IgM, IgD and IgZ, were cloned from mandarin fish (Siniperca chuatsi) recently. In this study the distribution of these three kinds of Ig-producing cells in lymphoid-related tissues as head kidney, spleen, gill and intestine were investigated by using in situ hybridization, and their transcriptional changes were also analyzed by quantitative real-time PCR during 8 weeks after immunization. IgM-producing cells could be detected obviously and abundantly in all the tissues examined. A few numbers of IgD and IgZ positive cells were both detected in head kidney and spleen. IgZ positive cells could be detected in gill moderately while IgD showed negative results, otherwise no IgD or IgZ positive cells could be detected in intestine. After stimulated with bacterial pathogen Flavobacterium columnare G(4), the transcripts of these three Ig genes exhibited quite different kinetics. Significantly increased transcription of IgM gene was observed in almost all the tissues examined especially in boosted group. In contrast with IgM, seldom strong increase was examined for IgD and IgZ genes. For IgD, it seemed that the first injection could stimulate the immune response easier, since in almost all the tissues significant increase was detected at 1 or 2 weeks after injection. For IgZ, boosted injection could not enlarge the up-regulation of gene expression of first injection. This is the first case to report the transcriptional kinetics of three Ig genes in teleost after bacterin immunization. (C) 2008 Elsevier B.V. All rights reserved. Article in Journal/Newspaper Atlantic salmon Institute of Hydrobiology, Chinese Academy of Sciences: IHB OpenIR Aquaculture 288 1-2 14 21 |