Enhanced grass carp reovirus resistance of Mx-transgenic rare minnow (Gobiocypris rarus)
In the interferon-induced antiviral mechanisms, the Mx pathway is one of the most powerful. Mx proteins have direct antiviral activity and inhibit a wide range of viruses by blocking an early stage of the viral genome replication cycle. However, antiviral activity of piscine Mx remains unclear in vi...
Published in: | Fish & Shellfish Immunology |
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Main Authors: | , , , , , , |
Format: | Article in Journal/Newspaper |
Language: | English |
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2009
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Online Access: | http://ir.ihb.ac.cn/handle/152342/7708 https://doi.org/10.1016/j.fsi.2008.12.007 |
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ftchinacadsciihb:oai:ir.ihb.ac.cn:152342/7708 |
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record_format |
openpolar |
institution |
Open Polar |
collection |
Institute of Hydrobiology, Chinese Academy of Sciences: IHB OpenIR |
op_collection_id |
ftchinacadsciihb |
language |
English |
topic |
Rare Minnow (Gobiocypris Rarus) Mx Gene Grass Carp Reovirus Transgenic Fish Antiviral Activity Gene Cloning Fisheries Immunology Marine & Freshwater Biology Veterinary Sciences Science & Technology Life Sciences & Biomedicine VESICULAR STOMATITIS-VIRUS IN-VITRO INHIBITION INFLUENZA-VIRUS ATLANTIC SALMON EXPRESSION ANALYSIS SOLE AQUABIRNAVIRUS CELL-LINES PROTEIN GENE |
spellingShingle |
Rare Minnow (Gobiocypris Rarus) Mx Gene Grass Carp Reovirus Transgenic Fish Antiviral Activity Gene Cloning Fisheries Immunology Marine & Freshwater Biology Veterinary Sciences Science & Technology Life Sciences & Biomedicine VESICULAR STOMATITIS-VIRUS IN-VITRO INHIBITION INFLUENZA-VIRUS ATLANTIC SALMON EXPRESSION ANALYSIS SOLE AQUABIRNAVIRUS CELL-LINES PROTEIN GENE Su, Jianguo Yang, Chunrong Zhu, Zuoyan Wang, Yaping Jang, Songhun Liao, Lanjie Wang, YP, Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China Enhanced grass carp reovirus resistance of Mx-transgenic rare minnow (Gobiocypris rarus) |
topic_facet |
Rare Minnow (Gobiocypris Rarus) Mx Gene Grass Carp Reovirus Transgenic Fish Antiviral Activity Gene Cloning Fisheries Immunology Marine & Freshwater Biology Veterinary Sciences Science & Technology Life Sciences & Biomedicine VESICULAR STOMATITIS-VIRUS IN-VITRO INHIBITION INFLUENZA-VIRUS ATLANTIC SALMON EXPRESSION ANALYSIS SOLE AQUABIRNAVIRUS CELL-LINES PROTEIN GENE |
description |
In the interferon-induced antiviral mechanisms, the Mx pathway is one of the most powerful. Mx proteins have direct antiviral activity and inhibit a wide range of viruses by blocking an early stage of the viral genome replication cycle. However, antiviral activity of piscine Mx remains unclear in vivo. In the present study, an Mx-like gene was cloned, characterized and gene-transferred in rare minnow Gobiocypris rarus, and its antiviral activity was confirmed in vivo. The full length of the rare minnow Mx-like cDNA is 2241 bp in length and encodes a polypeptide of 625 amino acids with an estimated molecular mass of 70.928 kDa and a predicted isoelectric point of 7.33. Analysis of the deduced amino acid sequence indicated that the mature peptide contains an amino-terminal tripartite GTP-binding motif, a dynamin family signature sequence, a GTPase effector domain and two carboxy-terminal leucine zipper motifs, and is the most similar to the crucian carp (Carassius auratus) Mx3 sequence with an identity of 89%. Both P0 and F1 generations of Mx-transgenic rare minnow demonstrated very significantly high survival rate to GCRV infection (P < 0.01). The mRNA expression of Mx gene was consistent with survival rate in F1 generation. The virus yield was also concurrent with survival time using electron microscope technology. Rare minnow has Mx gene(s) of its own but introducing more Mx gene improves their resistance to GCRV. Mx-transgenic rare minnow might contribute to control the GCRV diseases. (C) 2008 Published by Elsevier Ltd. In the interferon-induced antiviral mechanisms, the Mx pathway is one of the most powerful. Mx proteins have direct antiviral activity and inhibit a wide range of viruses by blocking an early stage of the viral genome replication cycle. However, antiviral activity of piscine Mx remains unclear in vivo. In the present study, an Mx-like gene was cloned, characterized and gene-transferred in rare minnow Gobiocypris rarus, and its antiviral activity was confirmed in vivo. The full length of the rare minnow Mx-like cDNA is 2241 bp in length and encodes a polypeptide of 625 amino acids with an estimated molecular mass of 70.928 kDa and a predicted isoelectric point of 7.33. Analysis of the deduced amino acid sequence indicated that the mature peptide contains an amino-terminal tripartite GTP-binding motif, a dynamin family signature sequence, a GTPase effector domain and two carboxy-terminal leucine zipper motifs, and is the most similar to the crucian carp (Carassius auratus) Mx3 sequence with an identity of 89%. Both P0 and F1 generations of Mx-transgenic rare minnow demonstrated very significantly high survival rate to GCRV infection (P < 0.01). The mRNA expression of Mx gene was consistent with survival rate in F1 generation. The virus yield was also concurrent with survival time using electron microscope technology. Rare minnow has Mx gene(s) of its own but introducing more Mx gene improves their resistance to GCRV. Mx-transgenic rare minnow might contribute to control the GCRV diseases. (C) 2008 Published by Elsevier Ltd. |
format |
Article in Journal/Newspaper |
author |
Su, Jianguo Yang, Chunrong Zhu, Zuoyan Wang, Yaping Jang, Songhun Liao, Lanjie Wang, YP, Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China |
author_facet |
Su, Jianguo Yang, Chunrong Zhu, Zuoyan Wang, Yaping Jang, Songhun Liao, Lanjie Wang, YP, Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China |
author_sort |
Su, Jianguo |
title |
Enhanced grass carp reovirus resistance of Mx-transgenic rare minnow (Gobiocypris rarus) |
title_short |
Enhanced grass carp reovirus resistance of Mx-transgenic rare minnow (Gobiocypris rarus) |
title_full |
Enhanced grass carp reovirus resistance of Mx-transgenic rare minnow (Gobiocypris rarus) |
title_fullStr |
Enhanced grass carp reovirus resistance of Mx-transgenic rare minnow (Gobiocypris rarus) |
title_full_unstemmed |
Enhanced grass carp reovirus resistance of Mx-transgenic rare minnow (Gobiocypris rarus) |
title_sort |
enhanced grass carp reovirus resistance of mx-transgenic rare minnow (gobiocypris rarus) |
publishDate |
2009 |
url |
http://ir.ihb.ac.cn/handle/152342/7708 https://doi.org/10.1016/j.fsi.2008.12.007 |
genre |
Atlantic salmon |
genre_facet |
Atlantic salmon |
op_relation |
FISH & SHELLFISH IMMUNOLOGY Su, Jianguo; Yang, Chunrong; Zhu, Zuoyan; Wang, Yaping; Jang, Songhun; Liao, Lanjie.Enhanced grass carp reovirus resistance of Mx-transgenic rare minnow (Gobiocypris rarus),FISH & SHELLFISH IMMUNOLOGY,2009,26(6):828-835 http://ir.ihb.ac.cn/handle/152342/7708 doi:10.1016/j.fsi.2008.12.007 |
op_doi |
https://doi.org/10.1016/j.fsi.2008.12.007 |
container_title |
Fish & Shellfish Immunology |
container_volume |
26 |
container_issue |
6 |
container_start_page |
828 |
op_container_end_page |
835 |
_version_ |
1766363626411655168 |
spelling |
ftchinacadsciihb:oai:ir.ihb.ac.cn:152342/7708 2023-05-15T15:33:09+02:00 Enhanced grass carp reovirus resistance of Mx-transgenic rare minnow (Gobiocypris rarus) Su, Jianguo Yang, Chunrong Zhu, Zuoyan Wang, Yaping Jang, Songhun Liao, Lanjie Wang, YP, Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China 2009-06-01 http://ir.ihb.ac.cn/handle/152342/7708 https://doi.org/10.1016/j.fsi.2008.12.007 英语 eng FISH & SHELLFISH IMMUNOLOGY Su, Jianguo; Yang, Chunrong; Zhu, Zuoyan; Wang, Yaping; Jang, Songhun; Liao, Lanjie.Enhanced grass carp reovirus resistance of Mx-transgenic rare minnow (Gobiocypris rarus),FISH & SHELLFISH IMMUNOLOGY,2009,26(6):828-835 http://ir.ihb.ac.cn/handle/152342/7708 doi:10.1016/j.fsi.2008.12.007 Rare Minnow (Gobiocypris Rarus) Mx Gene Grass Carp Reovirus Transgenic Fish Antiviral Activity Gene Cloning Fisheries Immunology Marine & Freshwater Biology Veterinary Sciences Science & Technology Life Sciences & Biomedicine VESICULAR STOMATITIS-VIRUS IN-VITRO INHIBITION INFLUENZA-VIRUS ATLANTIC SALMON EXPRESSION ANALYSIS SOLE AQUABIRNAVIRUS CELL-LINES PROTEIN GENE Article 期刊论文 2009 ftchinacadsciihb https://doi.org/10.1016/j.fsi.2008.12.007 2019-07-01T11:26:37Z In the interferon-induced antiviral mechanisms, the Mx pathway is one of the most powerful. Mx proteins have direct antiviral activity and inhibit a wide range of viruses by blocking an early stage of the viral genome replication cycle. However, antiviral activity of piscine Mx remains unclear in vivo. In the present study, an Mx-like gene was cloned, characterized and gene-transferred in rare minnow Gobiocypris rarus, and its antiviral activity was confirmed in vivo. The full length of the rare minnow Mx-like cDNA is 2241 bp in length and encodes a polypeptide of 625 amino acids with an estimated molecular mass of 70.928 kDa and a predicted isoelectric point of 7.33. Analysis of the deduced amino acid sequence indicated that the mature peptide contains an amino-terminal tripartite GTP-binding motif, a dynamin family signature sequence, a GTPase effector domain and two carboxy-terminal leucine zipper motifs, and is the most similar to the crucian carp (Carassius auratus) Mx3 sequence with an identity of 89%. Both P0 and F1 generations of Mx-transgenic rare minnow demonstrated very significantly high survival rate to GCRV infection (P < 0.01). The mRNA expression of Mx gene was consistent with survival rate in F1 generation. The virus yield was also concurrent with survival time using electron microscope technology. Rare minnow has Mx gene(s) of its own but introducing more Mx gene improves their resistance to GCRV. Mx-transgenic rare minnow might contribute to control the GCRV diseases. (C) 2008 Published by Elsevier Ltd. In the interferon-induced antiviral mechanisms, the Mx pathway is one of the most powerful. Mx proteins have direct antiviral activity and inhibit a wide range of viruses by blocking an early stage of the viral genome replication cycle. However, antiviral activity of piscine Mx remains unclear in vivo. In the present study, an Mx-like gene was cloned, characterized and gene-transferred in rare minnow Gobiocypris rarus, and its antiviral activity was confirmed in vivo. The full length of the rare minnow Mx-like cDNA is 2241 bp in length and encodes a polypeptide of 625 amino acids with an estimated molecular mass of 70.928 kDa and a predicted isoelectric point of 7.33. Analysis of the deduced amino acid sequence indicated that the mature peptide contains an amino-terminal tripartite GTP-binding motif, a dynamin family signature sequence, a GTPase effector domain and two carboxy-terminal leucine zipper motifs, and is the most similar to the crucian carp (Carassius auratus) Mx3 sequence with an identity of 89%. Both P0 and F1 generations of Mx-transgenic rare minnow demonstrated very significantly high survival rate to GCRV infection (P < 0.01). The mRNA expression of Mx gene was consistent with survival rate in F1 generation. The virus yield was also concurrent with survival time using electron microscope technology. Rare minnow has Mx gene(s) of its own but introducing more Mx gene improves their resistance to GCRV. Mx-transgenic rare minnow might contribute to control the GCRV diseases. (C) 2008 Published by Elsevier Ltd. Article in Journal/Newspaper Atlantic salmon Institute of Hydrobiology, Chinese Academy of Sciences: IHB OpenIR Fish & Shellfish Immunology 26 6 828 835 |