不同乾燥方式對銀杏葉抗氧化能力及酪胺酸酶活性之影響

本研究目的是探討不同乾燥方式對銀杏葉(Ginkgo biloba leaf)抗氧化能力及酪胺酸酶活性之影響。研究結果顯示，銀杏葉之一般成分，水分為74.15%，粗蛋白為3.80%，粗脂肪為2.41%，灰分為3.15%及碳水化合物為16.49%，每克銀杏葉中含有抗氧化營養素維生素C 1.08毫克、維生素E 2.29毫克、硒 76.20微克及鋅 4.15微克。不同乾燥方式處理銀杏葉之乙醇萃取物及銀杏葉水萃取物產率為5.67-25.40%及6.20-26.93%。有效成分方面，銀杏葉乙醇萃取物每毫克之總酚含量為24.07-60.00微克；不同乙醇萃取銀杏葉時間，以乙醇萃取12小時之總酚含量較高；不同...

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Main Authors:	陳緯諭, Wei-Yu Chen
Other Authors:	呂昆霖, 林美芳, 嘉南藥理科技大學：化妝品科技研究所
Format:	Thesis
Language:	Chinese English
Published:	2008
Subjects:	銀杏葉類黃酮類抗氧化酪胺酸酶 Arctic
Online Access:	https://ir.cnu.edu.tw/handle/310902800/9733 https://ir.cnu.edu.tw/bitstream/310902800/9733/4/index.html

id	ftchiananuniv:oai:ir.cnu.edu.tw:310902800/9733
record_format	openpolar
institution	Open Polar
collection	Chia Nan University of Pharmacy & Science Institutional Repository (CHNAIR)
op_collection_id	ftchiananuniv
language	Chinese English
topic	銀杏葉類黃酮類抗氧化酪胺酸酶
spellingShingle	銀杏葉類黃酮類抗氧化酪胺酸酶陳緯諭 Wei-Yu Chen 不同乾燥方式對銀杏葉抗氧化能力及酪胺酸酶活性之影響
topic_facet	銀杏葉類黃酮類抗氧化酪胺酸酶
description	本研究目的是探討不同乾燥方式對銀杏葉(Ginkgo biloba leaf)抗氧化能力及酪胺酸酶活性之影響。研究結果顯示，銀杏葉之一般成分，水分為74.15%，粗蛋白為3.80%，粗脂肪為2.41%，灰分為3.15%及碳水化合物為16.49%，每克銀杏葉中含有抗氧化營養素維生素C 1.08毫克、維生素E 2.29毫克、硒 76.20微克及鋅 4.15微克。不同乾燥方式處理銀杏葉之乙醇萃取物及銀杏葉水萃取物產率為5.67-25.40%及6.20-26.93%。有效成分方面，銀杏葉乙醇萃取物每毫克之總酚含量為24.07-60.00微克；不同乙醇萃取銀杏葉時間，以乙醇萃取12小時之總酚含量較高；不同乾燥方式處理銀杏葉乙醇萃取物，以室溫乾燥銀杏葉乙醇萃取物總酚含量較高；銀杏葉乙醇萃取物之類黃酮含量(檞皮素、異鼠李素及堪非黃酮醇)為每毫克萃取物中含8.00-26.56微克。銀杏葉水萃取物每毫克之總酚含量為10.69-39.07微克；不同溫度水萃取銀杏葉，以60℃水萃取銀杏葉之總酚含量較高；不同乾燥方式處理銀杏葉水萃取物以室溫乾燥銀杏葉水萃取物總酚含量較高；銀杏葉水萃取物之類黃酮含量(檞皮素、異鼠李素及堪非黃酮醇)為每毫克萃取物中含2.60-12.95微克。在抗氧化能力方面，商業配方(60毫克銀杏葉萃取物中包含14.4毫克黃酮糖苷及3.6毫克類萜類三內酯)、銀杏葉乙醇萃取物及銀杏葉水萃取物之DPPH自由基半數清除劑量分別為每毫升76.48微克、574.80-902.95微克及244.17-375.89微克；不同乾燥方式處理銀杏葉之DPPH自由基半數清除能力均以新鮮銀杏葉乙醇萃取物或新鮮銀杏葉水萃取物較佳。每毫克商業配方、銀杏葉乙醇萃取物及銀杏葉水萃取物之還原力分別相當91.21微克、20.00-28.63微克及19.29-28.09微克之維生素C含量；乾燥方式以室溫、熱風及冷凍乾燥銀杏葉乙醇萃取物或新鮮銀杏葉水萃取物及室溫乾燥銀杏葉水萃取物之還原力較佳。商業配方、銀杏葉乙醇萃取物及銀杏葉水萃取物之Trolox能力分別為每毫克80.56微克、5.90-13.73微克及7.95-12.21微克；乾燥方式以室溫乾燥銀杏葉乙醇萃取物及冷凍乾燥銀杏葉水萃取物之Trolox能力較佳。商業配方及銀杏葉乙醇萃取物之螯合亞鐵離子能力為相當36.91微克EDTA (ethylenediaminetetraacetic acid)及60.28-88.72微克EDTA；螯合亞鐵離子能力以銀杏葉乙醇萃取物高於商業配方。銀杏葉水萃取物之酪胺酸酶半數抑制劑量為每毫升19.34-38.98毫克；乾燥方式以新鮮銀杏葉水萃取物較佳。整體而言，以室溫乾燥方式處理銀杏葉能保留較多的有效成分，亦呈現較佳的抗氧化能力；酪胺酸酶之抑制能力則以新鮮銀杏葉抑制能力較佳。 The objective of the present study was to investigate the effect of drying methods on antioxidant capacity and tyrosinase activity of Ginkgo biloba leaf. The proximate composition of Ginkgo biloba leaf were 74.60%, 3.80%, 2.41%, 3.15% and 16.40%, respectively, for moisture, protein, fat, ash and carbohydrate. The leaves contained antioxidant nutrients for 1.8mg vitamin C, 2.29 mg vitamin E, 76.20 μg Se and 4.15 μg Zn per gram of homogenate. The total yields of ethanol or water Ginkgo biloba extracts by different drying methods were 5.67-25.40% or 6.20-26.93%. Total phenol contents of ethanol extracts were 24.07-60.00 μg per mg, and showed higher (p<0.05) phenolic compounds obtained from Ginkgo biloba leaves by ethanol extracted for 12 hours, the other by room temperature drying. The flavonoids (i.e. quercetin, isorharmnetin and kaempferol) concentrations ...
author2	呂昆霖林美芳嘉南藥理科技大學：化妝品科技研究所
format	Thesis
author	陳緯諭 Wei-Yu Chen
author_facet	陳緯諭 Wei-Yu Chen
author_sort	陳緯諭
title	不同乾燥方式對銀杏葉抗氧化能力及酪胺酸酶活性之影響
title_short	不同乾燥方式對銀杏葉抗氧化能力及酪胺酸酶活性之影響
title_full	不同乾燥方式對銀杏葉抗氧化能力及酪胺酸酶活性之影響
title_fullStr	不同乾燥方式對銀杏葉抗氧化能力及酪胺酸酶活性之影響
title_full_unstemmed	不同乾燥方式對銀杏葉抗氧化能力及酪胺酸酶活性之影響
title_sort	不同乾燥方式對銀杏葉抗氧化能力及酪胺酸酶活性之影響
publishDate	2008
url	https://ir.cnu.edu.tw/handle/310902800/9733 https://ir.cnu.edu.tw/bitstream/310902800/9733/4/index.html
genre	Arctic
genre_facet	Arctic
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spelling	ftchiananuniv:oai:ir.cnu.edu.tw:310902800/9733 2023-05-15T14:28:32+02:00 不同乾燥方式對銀杏葉抗氧化能力及酪胺酸酶活性之影響 Effects of drying methods on antioxidant capacity and tyrosinase activity of Ginkgo biloba leaf 陳緯諭 Wei-Yu Chen 呂昆霖林美芳嘉南藥理科技大學：化妝品科技研究所 2008 142 bytes application/octet-stream https://ir.cnu.edu.tw/handle/310902800/9733 https://ir.cnu.edu.tw/bitstream/310902800/9733/4/index.html zh_TW en_US chi eng 校內外均一年後公開 1. Le Bars P. L., Katz M. M., Berman N., Itil T. M., Freedman A. M., Schatzberg A. F., A placebo-controlled, double-blind, randomized trial of an extract of Ginkgo biloba for dementia. North American EGb Study Group, The Journal of the American Medical Association, 278: 1327-1332, 1997. 2. Wang H., Ng T. B., Ginkbilobin, novel antifungal protein from Ginkgo biloba seeds with sequence similarity to Embryo-Abundant protein, Biochemical and Biophysical Research Communications, 279: 407-411, 2000. 3. Xin W., Wei T., Chen C., Ni Y., Zhao B., Hou J., Mechanisms of apoptosis in rat cerebellar granule cells induced by hydroxyl radicals and the effects of EGb761 and its constituents, Toxicology, 148: 103-10, 2000. 4. Cheng S. M., Yang S. P., Ho L. J., Tsao T. P., Juan T. Y., Chang D. M., Chang S. Y., Lai J. H., Down-regulation of c-jun N-terminal kinase-activator protein-1 signaling pathway by Ginkgo biloba extract in human peripheral blood T cells, Biochem Pharmacol, 66: 679-689, 2003. 5. Wang Q., Zhao W. Z., Ma C. G., Protective effects of Ginkgo biloba extract on gastric mucosa, Acta Pharmacologica Sinica, 21: 1153-1156, 2000. 6. Soybir G., Koksoy F., Ekiz F., Yalcn O., Fincan K., Haklar G., Yuksel M., The effects of free oxygen radical scavenger and platelet-activating factor antagonist agents in experimental acute pancreatitis, Pancreas, 19(2): 143-149, 1999. 7. 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X., Inhibitory effects of 4-chlorosalicylic acid on mushroom tryosinase and its antimicrobial activities, Food Chemistry, 107: 797-803, 2008. 58. 李穩宏、王鋒、李多偉、高麗雅、韓楓，超聲提取銀杏外種皮中黃酮類物質工藝條件。西北大學學報，35(4)，416-418頁，2005。 59. 莫曉燕、徐靜、張安寧，銀杏中種皮提取物抗氧化活性的研究。西北農林科技大學學報(自然科學版)，33(11)，pp.83-88頁，2005。 https://ir.cnu.edu.tw/handle/310902800/9733 https://ir.cnu.edu.tw/bitstream/310902800/9733/4/index.html 銀杏葉類黃酮類抗氧化酪胺酸酶 thesis 2008 ftchiananuniv 2022-05-15T05:25:33Z 本研究目的是探討不同乾燥方式對銀杏葉(Ginkgo biloba leaf)抗氧化能力及酪胺酸酶活性之影響。研究結果顯示，銀杏葉之一般成分，水分為74.15%，粗蛋白為3.80%，粗脂肪為2.41%，灰分為3.15%及碳水化合物為16.49%，每克銀杏葉中含有抗氧化營養素維生素C 1.08毫克、維生素E 2.29毫克、硒 76.20微克及鋅 4.15微克。不同乾燥方式處理銀杏葉之乙醇萃取物及銀杏葉水萃取物產率為5.67-25.40%及6.20-26.93%。有效成分方面，銀杏葉乙醇萃取物每毫克之總酚含量為24.07-60.00微克；不同乙醇萃取銀杏葉時間，以乙醇萃取12小時之總酚含量較高；不同乾燥方式處理銀杏葉乙醇萃取物，以室溫乾燥銀杏葉乙醇萃取物總酚含量較高；銀杏葉乙醇萃取物之類黃酮含量(檞皮素、異鼠李素及堪非黃酮醇)為每毫克萃取物中含8.00-26.56微克。銀杏葉水萃取物每毫克之總酚含量為10.69-39.07微克；不同溫度水萃取銀杏葉，以60℃水萃取銀杏葉之總酚含量較高；不同乾燥方式處理銀杏葉水萃取物以室溫乾燥銀杏葉水萃取物總酚含量較高；銀杏葉水萃取物之類黃酮含量(檞皮素、異鼠李素及堪非黃酮醇)為每毫克萃取物中含2.60-12.95微克。在抗氧化能力方面，商業配方(60毫克銀杏葉萃取物中包含14.4毫克黃酮糖苷及3.6毫克類萜類三內酯)、銀杏葉乙醇萃取物及銀杏葉水萃取物之DPPH自由基半數清除劑量分別為每毫升76.48微克、574.80-902.95微克及244.17-375.89微克；不同乾燥方式處理銀杏葉之DPPH自由基半數清除能力均以新鮮銀杏葉乙醇萃取物或新鮮銀杏葉水萃取物較佳。每毫克商業配方、銀杏葉乙醇萃取物及銀杏葉水萃取物之還原力分別相當91.21微克、20.00-28.63微克及19.29-28.09微克之維生素C含量；乾燥方式以室溫、熱風及冷凍乾燥銀杏葉乙醇萃取物或新鮮銀杏葉水萃取物及室溫乾燥銀杏葉水萃取物之還原力較佳。商業配方、銀杏葉乙醇萃取物及銀杏葉水萃取物之Trolox能力分別為每毫克80.56微克、5.90-13.73微克及7.95-12.21微克；乾燥方式以室溫乾燥銀杏葉乙醇萃取物及冷凍乾燥銀杏葉水萃取物之Trolox能力較佳。商業配方及銀杏葉乙醇萃取物之螯合亞鐵離子能力為相當36.91微克EDTA (ethylenediaminetetraacetic acid)及60.28-88.72微克EDTA；螯合亞鐵離子能力以銀杏葉乙醇萃取物高於商業配方。銀杏葉水萃取物之酪胺酸酶半數抑制劑量為每毫升19.34-38.98毫克；乾燥方式以新鮮銀杏葉水萃取物較佳。整體而言，以室溫乾燥方式處理銀杏葉能保留較多的有效成分，亦呈現較佳的抗氧化能力；酪胺酸酶之抑制能力則以新鮮銀杏葉抑制能力較佳。 The objective of the present study was to investigate the effect of drying methods on antioxidant capacity and tyrosinase activity of Ginkgo biloba leaf. The proximate composition of Ginkgo biloba leaf were 74.60%, 3.80%, 2.41%, 3.15% and 16.40%, respectively, for moisture, protein, fat, ash and carbohydrate. The leaves contained antioxidant nutrients for 1.8mg vitamin C, 2.29 mg vitamin E, 76.20 μg Se and 4.15 μg Zn per gram of homogenate. The total yields of ethanol or water Ginkgo biloba extracts by different drying methods were 5.67-25.40% or 6.20-26.93%. Total phenol contents of ethanol extracts were 24.07-60.00 μg per mg, and showed higher (p<0.05) phenolic compounds obtained from Ginkgo biloba leaves by ethanol extracted for 12 hours, the other by room temperature drying. The flavonoids (i.e. quercetin, isorharmnetin and kaempferol) concentrations ... Thesis Arctic Chia Nan University of Pharmacy & Science Institutional Repository (CHNAIR)

不同乾燥方式對銀杏葉抗氧化能力及酪胺酸酶活性之影響

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