河川底泥中六氯苯污染之環境復育研究

本研究在測試台灣與泰國底泥環境之原生菌種的脫氯能力，在台灣地區採集二仁溪及後勁溪兩條河川之底泥進行六氯苯(Hexachlorobenzene, HCB)脫氯測試，在各種培養條件下其結果均顯示遲滯期甚長(>120天)，且無法將HCB完全的降解，只有在直接添加HCB粉未的這組實驗中有較短的遲滯期(30及90天)，但仍無法將HCB完全脫氯降解，而在多氯聯苯的實驗中，經120天的培養也都沒有脫氯作用發生，另外在泰國運河的底泥中進行HCB分析，結果顯示有4個點發現HCB和1.3.5-三氯苯(1.3.5-trichlorobenzene, 1.3.5-TCB)，因為1.3.5-TCB皆由高氯數之氯苯...

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Main Authors:	王炯強, Chiung-Chiang Wang
Other Authors:	陳意銘, 嘉南藥理科技大學：環境工程與科學系碩士班
Format:	Thesis
Language:	Chinese English
Published:	2006
Subjects:	泰國運河脫氯作用多氯聯苯六氯苯 Thai Canal dechlorintion hexachlorobenzene polychlorinated biphenyls Arctic
Online Access:	https://ir.cnu.edu.tw/handle/310902800/9094 https://ir.cnu.edu.tw/bitstream/310902800/9094/3/index.html

id	ftchiananuniv:oai:ir.cnu.edu.tw:310902800/9094
record_format	openpolar
institution	Open Polar
collection	Chia Nan University of Pharmacy & Science Institutional Repository (CHNAIR)
op_collection_id	ftchiananuniv
language	Chinese English
topic	泰國運河脫氯作用多氯聯苯六氯苯 Thai Canal dechlorintion hexachlorobenzene polychlorinated biphenyls
spellingShingle	泰國運河脫氯作用多氯聯苯六氯苯 Thai Canal dechlorintion hexachlorobenzene polychlorinated biphenyls 王炯強 Chiung-Chiang Wang 河川底泥中六氯苯污染之環境復育研究
topic_facet	泰國運河脫氯作用多氯聯苯六氯苯 Thai Canal dechlorintion hexachlorobenzene polychlorinated biphenyls
description	本研究在測試台灣與泰國底泥環境之原生菌種的脫氯能力，在台灣地區採集二仁溪及後勁溪兩條河川之底泥進行六氯苯(Hexachlorobenzene, HCB)脫氯測試，在各種培養條件下其結果均顯示遲滯期甚長(>120天)，且無法將HCB完全的降解，只有在直接添加HCB粉未的這組實驗中有較短的遲滯期(30及90天)，但仍無法將HCB完全脫氯降解，而在多氯聯苯的實驗中，經120天的培養也都沒有脫氯作用發生，另外在泰國運河的底泥中進行HCB分析，結果顯示有4個點發現HCB和1.3.5-三氯苯(1.3.5-trichlorobenzene, 1.3.5-TCB)，因為1.3.5-TCB皆由高氯數之氯苯化合物降解而來，這顯示出泰國運河底泥微生物有自行脫氯能力，因此在泰國研究中則繼續以運河底泥中的厭氧微生物來進行HCB的還原脫氯作用，實驗分兩個階段，第一階段是由10點取得底泥微生物並應用於脫氯實驗上，過程中使用底泥加河水之底泥水再添加5g/L的yeast extract (YE)或是不添加。所有點中之HCB在14天後開始脫氯， 28-42天完全脫氯，未添加YE部份則在14至28天後有較緩慢的脫氯活動。事先添加養份和厭氧處理之組別在7天後，運河底泥微生物即有HCB之脫氯能力，並在6星期內完全脫氯，第二階段為評估HCB在經前處理及未處理的新鮮底泥水中之脫氯作用，結果顯示，底泥與水比例越高的組別其脫氯能力越強，底泥水不論過濾與否，都有HCB脫氯作用發生，另外，底泥水經前厭氧處理的時間越長，則對HCB的脫氯能力越佳。本研究結果顯示HCB脫氯菌廣闊的分佈在泰國運河沿岸的底泥，而且也顯示HCB污染的運河底泥之中有環境復育的可行性。 In order to investigate the hexachlorobenzene (HCB) dechlorintion ability of indigenous microbes in Taiwan and Thailand, river and canal sediments were collected and introduced in the HCB dechlorination tests. Most sets by using anaerobic microbes from Er-Jen River and Ho-Tsin River slowly dechlorinated HCB with a longer lag phase more than 120 days, and only those sets that added powder-like HCB showed short lag phase. Futhermore, these microbes cannot dechlorinate polychlorinated biphenyls within 120 days of incubation. In contrast to the weak HCB dechlorination ability of Taiwan river sediment anaerobes, indigenous microbes from Thai canal sediments showed more active dechlorination. The reason for Thai canal bacteria possessing novel HCB dechlorination potential might because the Canal was found contaminated by HCB. The HCB dechlorination experiments by Thai Canal microbes included two stages. In the first stage, sediments from 10 different sites were collected and the HCB dechlorination ability of the sediment microbes was also tested. In the sets with yeast extracts (YE), all 10 sites of microbes showed dechlorination activity after 14 days of incubation and completed the dechlorination within 28 ~ 42 days. In the sets without YE, the lag phase was about 14 ~ 28 days. In the second stage, HCB ...
author2	陳意銘嘉南藥理科技大學：環境工程與科學系碩士班
format	Thesis
author	王炯強 Chiung-Chiang Wang
author_facet	王炯強 Chiung-Chiang Wang
author_sort	王炯強
title	河川底泥中六氯苯污染之環境復育研究
title_short	河川底泥中六氯苯污染之環境復育研究
title_full	河川底泥中六氯苯污染之環境復育研究
title_fullStr	河川底泥中六氯苯污染之環境復育研究
title_full_unstemmed	河川底泥中六氯苯污染之環境復育研究
title_sort	河川底泥中六氯苯污染之環境復育研究
publishDate	2006
url	https://ir.cnu.edu.tw/handle/310902800/9094 https://ir.cnu.edu.tw/bitstream/310902800/9094/3/index.html
genre	Arctic
genre_facet	Arctic
op_relation	校內外完全公開參考文獻 1. Simonich, S. L., and Hites, R. A, “ Global distribution of persistent organochlorine compounds”, Science , 269: 1851-1854, 1995 2. Fellin, P., Barrie, L. A., Dougherty, D., Toom, D., Muir, D., Grift, N., Lockhart, L., and Billeck, B, “Air monitoring in the arctic: results for selected persistent organic pollutants for 1992”, Environ. Toxicol. Chem., 15: 253-261, 1996 3. Pearson, C.R., ”Halogenated aromatics,” The handbook of environmental chemistry”, O. Huntzinger,ed., Vol 3, Part B, Springer, New York, 1982. 4. Weber, K., and Goerke, H,” Organochlorine compounds in fish off the Antarctic Peninsula”, Chemosphere 33: 377-392, 1996 5. Kuhn, W.; Brauch, J.-Fresenius Z. Anal,” Vinyl Chloride: Still a Cause for Concern”, Chem., 330:324-325, 1988 6. Kypke-Hutter, K.,Vogelgesang, J., M.,” The origin of contamination of fish from the River Neckar with hexachlorobenzene octachlorostyrene and pentachlorobenzene: formation in an industrial process. 1. The course of the contamination in the upper section of the River Neckar Z”, Unters. Forsh., 182: 464-470, 1986 7. Jacobus E. M. Beurskens, Connle G. C. Dekker, ” Dechlorinayion of Chlorinated Benzenes by an Anaerobic Microbial Consortium That Selectively Mediates the Thermodynamic Most Favorable Reaction”, Environ Sci. Technol., 25: 701-706, 1994 8. Bestteti, G., Galli, B., Leoni, F. Pelizzoni, and G. Sello,” Regioselective hydroxylation of chlorobenzene and chlorophenols by a Pseudomonas pudita”, Appl. Microbiol. Biotechnol., 37: 260-263, 1992 9. De Bont, J. A. M.,Vorage, M. J. A.W.,Hartmans, S., and Van Den Tweel, w.t.j, ” Microbial degradation of 1,3- dichlorobenzene”, Appl. Environ. Microbiol., 52: 677-680, 1986. 10. Haiger, B. E., Pettigrew, C.A., and Spain J. C,” Biodegradation of mixtures of substituted benzenes by Psudomonas sp.Strain JS150”, Appl. Environ. Microbiol., 58: 348-353, 1992. 11. Kuhn, E. P., A. J. B. Zehnder., and R. P. Schwarzenbach, “ Microbial transformation of river water to ground water: labolatory column studies”, Environ. Sci. Technol., 19: 961-968, 1985. 12. Holliger C., Schraa, G., Stams, A. J. M., and Zehnder, A.J.B, ” Enrichment and properties of an anaerobic mixed culture reductively dechlorinating 1,2,3-trichlorobenzene to 1,3-dichlorobenzene”, Applied and Environmental Microbiology., 58: 1636-1644, 1992 13. Chang B.V., Chen, I. M., Yuan, S. Y., and Wang Y.S, ” Reductive dechlorination of Hexachlorobenzene by an anaerobic mixed culture”, Water Air Soil poll., 100, 25, 1997 14. Quensen J. f. Ⅲ, Boyd, S. A.,Tiedje, J.M, ” Dechlorination of four commercial polychlorinated biphenyls mixtures(Aroclors) by anaerobic microorganism from sediments”, Appl. Enverion.Microbiol., 56: 2360-2369, 1990 15. Fathepure, B. Z., Tiedje, J. M., and Boyd, S. A, “ Reductive dechlorination of hexachlorobenzene to tri- and dichlorobenzenes in anaerobic sewage sludge”, Appl. Environ. Microbiol., 54: 327-330, 1988 16. Nowak, J., Kirsch, N.H., Hegemann, W., and Stan, H. J, “ Total reductive dechlorination of chlorobenzenes to benzene by a methanogenic mixed culture enriched from Saale river sediment”, Appl. Microbiol. Biotechnol., 45: 700-709, 1996 17. Chen, I.M., Chang, F. C., and Wang, Y. S,“ Correlation of gas chromatographic properties of chlorobenzenes and polychlorinated biphenyls with the occurrence of reductive dechlorination by untamed microorganisms”, Chemosphere, 45 (2): 223-229, 2001 18. Chen, I.M., Chang, B. V., Yuan, S. Y., and Wang, Y. S,” Reductive dechlorination of hexachlorobenzene under various additions. Water, Air, and Soil Poll. 139, 61-74, 2002 19. Builey, E,”Global hexachlorobenzene emissions”, Chemosphere., 43: 167-182, 2001 20. 陳國樹，厭氧菌分解氯酚化合物及金屬離子影響之研究碩士論文，東吳微生物研究所，1993 21. Chang B.V.,SU C. J.,Yuan S. Y, “ Microbial Hexachlorobenzene dechlorination under three reducing conditions”, Chemosphere, 36: 2721-2730, 1998. 22. Kohring, G. W., J. E. 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J, ” Polychlorinated biphenyls in sediments and soil along the Erh-Jen River”, Master Thesis, Tainan, Taiwan., National Cheng Kung university, 1991 28. Chen, I. M., Chang F.C. and Wang Y.S,” Reductive dechlorination of Hexachlorobenzene and Polychlorinated biphenyls in anaerobic sediments from tropical rivers with enrichment”, Proceeding of the Symposium on Chlorinated Dioxins and Related Compounds, 17th, 33, 189., Indianapolis,Indiana, 1997. 29. 陳健民著，環境毒物學，初版，台北縣中和市，新文京開發出版社有限公司 315-355，2002 30. 陳意銘，馴化厭氧菌對六氯苯還原性脫氯作用影響因子之探討，碩士論文，私立東吳大學微生物學研究所，1994。 31. C. Lamglolis, and H. Sloterdijk,” Contamination of Lake Saint-Pierre(St. Lawrence River) by organic and inorganic pollutants”, Rev. Sic. Eau, 2: 659-670, 1989. 32. Brahushi F., Dorfler, U.,Schroll, R., Munch, J.C,” Stimulatiom of reductive dechlorination of hexachlorobenzene in soil by inducing the native microbial activity”, Chemosphere, 55: 1477-1484, 2004 33. 盧志人，地下水的整治污染，國立編譯館，1998。 34. Suthan S. Suthersan,” Remediation engineering desing concepts” CRC Press, Inc, 1996. 35. Peikang Jin and Sanjoy K,” Bhattacharya. Anaerobic removal of pentachlorophenol in presence of Zinc”, Journal of Environmental Engineering, Vol, 122: 590-598, 1996 36. Chen, I. M., Chang, F.C., and Wang, Y.S,” Correlation of gas chromatographic properties of chlorobenzenes and polychlorinated biphenyls with the occurrence of reductive dechlorination by untamed microorganisms”, Chemosphere, 45(2): 223-229, 2001. 37. Brown J.F., Wagner, R.E., Feng, H., Bedard, D. L., Brennan, M. J.,Carnahan, J. C.,May,R .J,” Envrionmental dechlorination of PCBs.Envrion.”, Toxicol. Chem, 6: 579-593, 1987 38. Frank M. Dunnivant, Anne L. Polansky and Alan W. Elzerman,” Persistence and distribution of PCBs in the sediments of a reservoir(Lake Hartwell, South Carolina)”, Bull. Environ. Contam. Toxicol, 43: 870-878, 1989 39. 劉孟駮，不同濃度氯酚化合物在汙泥中之厭氧分解，東吳微生物碩士論文，1992 40. Morris, P. J., W. W. Mohn, J F. Quensen Ⅲ, J. M. Tiedje, and S. A. 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C,” Initiation of [36Cl] hexachlorobenzene dechlorination in three different soils under artificially induced anaerobic conditions”, Appl. Microbiol. Biotechnol, 48: 115-120. 1997 46. Chen, I. M., Chang, Y. F., and Lin, H,” Microbial Dechlorination of hexachlorobenzene by untamed sediment microorganisms in Taiwan”, Practice Periodical of Hazardous, Toxic, and Radioactive Waste Management 8(2): 73-78, 2004 47. 行政院環保署 48. Sugiura, K., M.Kitamura, E. Matsumoto, and M. Goto,“ polychlorinated biphenyls(PCBs) in sediments of Tokyo Bay”, Japan. ARCH. Envrion. Contam. Toxicol, 15: 69-76, 1986 49. Gagnon, M. M., and J. J. Dodson,” Congener-specific analysis of the accumulation of polychlorinated biphenyls(PCBs) by aquatic organisms in the maximum turbidity zone of the St”, Lawrence Estuary, Quebec, Canada, Sci. total. Environ, 97/98：739-759, 1990. 50. Brannon, J. M., T. E. Myers, D. Gunnison, and C.B.Price,” Nonconstant polychlorinated biphenyls partitioning in new Bedford harbor sediment during sequential batch leaching”, Environ. Sci. Technol, 25：1082-1087, 1991 https://ir.cnu.edu.tw/handle/310902800/9094 https://ir.cnu.edu.tw/bitstream/310902800/9094/3/index.html
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spelling	ftchiananuniv:oai:ir.cnu.edu.tw:310902800/9094 2023-05-15T14:28:29+02:00 河川底泥中六氯苯污染之環境復育研究 Environmental remediation of HCB contaminated river sediment 王炯強 Chiung-Chiang Wang 陳意銘嘉南藥理科技大學：環境工程與科學系碩士班 2006 application/pdf 0 bytes https://ir.cnu.edu.tw/handle/310902800/9094 https://ir.cnu.edu.tw/bitstream/310902800/9094/3/index.html zh_TW en-US en_US chi eng 校內外完全公開參考文獻 1. Simonich, S. L., and Hites, R. A, “ Global distribution of persistent organochlorine compounds”, Science , 269: 1851-1854, 1995 2. Fellin, P., Barrie, L. A., Dougherty, D., Toom, D., Muir, D., Grift, N., Lockhart, L., and Billeck, B, “Air monitoring in the arctic: results for selected persistent organic pollutants for 1992”, Environ. Toxicol. Chem., 15: 253-261, 1996 3. Pearson, C.R., ”Halogenated aromatics,” The handbook of environmental chemistry”, O. Huntzinger,ed., Vol 3, Part B, Springer, New York, 1982. 4. Weber, K., and Goerke, H,” Organochlorine compounds in fish off the Antarctic Peninsula”, Chemosphere 33: 377-392, 1996 5. Kuhn, W.; Brauch, J.-Fresenius Z. Anal,” Vinyl Chloride: Still a Cause for Concern”, Chem., 330:324-325, 1988 6. Kypke-Hutter, K.,Vogelgesang, J., M.,” The origin of contamination of fish from the River Neckar with hexachlorobenzene octachlorostyrene and pentachlorobenzene: formation in an industrial process. 1. The course of the contamination in the upper section of the River Neckar Z”, Unters. Forsh., 182: 464-470, 1986 7. Jacobus E. M. Beurskens, Connle G. C. Dekker, ” Dechlorinayion of Chlorinated Benzenes by an Anaerobic Microbial Consortium That Selectively Mediates the Thermodynamic Most Favorable Reaction”, Environ Sci. Technol., 25: 701-706, 1994 8. Bestteti, G., Galli, B., Leoni, F. Pelizzoni, and G. Sello,” Regioselective hydroxylation of chlorobenzene and chlorophenols by a Pseudomonas pudita”, Appl. Microbiol. Biotechnol., 37: 260-263, 1992 9. De Bont, J. A. M.,Vorage, M. J. A.W.,Hartmans, S., and Van Den Tweel, w.t.j, ” Microbial degradation of 1,3- dichlorobenzene”, Appl. Environ. Microbiol., 52: 677-680, 1986. 10. Haiger, B. E., Pettigrew, C.A., and Spain J. C,” Biodegradation of mixtures of substituted benzenes by Psudomonas sp.Strain JS150”, Appl. Environ. Microbiol., 58: 348-353, 1992. 11. Kuhn, E. P., A. J. B. Zehnder., and R. P. Schwarzenbach, “ Microbial transformation of river water to ground water: labolatory column studies”, Environ. Sci. Technol., 19: 961-968, 1985. 12. Holliger C., Schraa, G., Stams, A. J. M., and Zehnder, A.J.B, ” Enrichment and properties of an anaerobic mixed culture reductively dechlorinating 1,2,3-trichlorobenzene to 1,3-dichlorobenzene”, Applied and Environmental Microbiology., 58: 1636-1644, 1992 13. Chang B.V., Chen, I. M., Yuan, S. Y., and Wang Y.S, ” Reductive dechlorination of Hexachlorobenzene by an anaerobic mixed culture”, Water Air Soil poll., 100, 25, 1997 14. Quensen J. f. Ⅲ, Boyd, S. A.,Tiedje, J.M, ” Dechlorination of four commercial polychlorinated biphenyls mixtures(Aroclors) by anaerobic microorganism from sediments”, Appl. Enverion.Microbiol., 56: 2360-2369, 1990 15. Fathepure, B. Z., Tiedje, J. M., and Boyd, S. A, “ Reductive dechlorination of hexachlorobenzene to tri- and dichlorobenzenes in anaerobic sewage sludge”, Appl. Environ. Microbiol., 54: 327-330, 1988 16. Nowak, J., Kirsch, N.H., Hegemann, W., and Stan, H. J, “ Total reductive dechlorination of chlorobenzenes to benzene by a methanogenic mixed culture enriched from Saale river sediment”, Appl. Microbiol. Biotechnol., 45: 700-709, 1996 17. Chen, I.M., Chang, F. C., and Wang, Y. S,“ Correlation of gas chromatographic properties of chlorobenzenes and polychlorinated biphenyls with the occurrence of reductive dechlorination by untamed microorganisms”, Chemosphere, 45 (2): 223-229, 2001 18. Chen, I.M., Chang, B. V., Yuan, S. Y., and Wang, Y. S,” Reductive dechlorination of hexachlorobenzene under various additions. Water, Air, and Soil Poll. 139, 61-74, 2002 19. Builey, E,”Global hexachlorobenzene emissions”, Chemosphere., 43: 167-182, 2001 20. 陳國樹，厭氧菌分解氯酚化合物及金屬離子影響之研究碩士論文，東吳微生物研究所，1993 21. Chang B.V.,SU C. J.,Yuan S. Y, “ Microbial Hexachlorobenzene dechlorination under three reducing conditions”, Chemosphere, 36: 2721-2730, 1998. 22. Kohring, G. W., J. E. Rogers., and J. Wiegel,” Anaerobic biodegradation of 2,4-chlorophenol in fresh water lake sediments at different temperatures”, Appl, Environ, Microbiol, 53: 348-353, 1989. 23. Larsen, S., H. V. Hendriksen, and B. K. Ahring,” Potentail for thermopilic(50℃) anaerobic dechlorination of pentachlorophenol in different ecosystem”, Appl, Environ, Microbiol, 57: 2085-2096, 1991. 24. Tiedje, J. M., J. F. Quensen Ⅲ., J. C. Sanford. J. P. Schimel, and S. A. Boyd,” Microbiol reductive dechlorination of PCB” Section 15. In Pacific Basin Conferece on hazardous Wastes., 1992 25. Valo, R., J. Apajalahti, and M. S. Sulonen.,” Studies on the physiology of microbial degradation of pentachlorophenol” Appl, Environ, Microbiol, 21: 313-319, 1985. 26. Pavlostathis, S. G.,and P. Zhuang,“ Reductive dechlorination of chloroalkenes on microcosms developed with a field contaminated soil” Chemosphere, 27: 585-595, 1993 27. Hu A. J, ” Polychlorinated biphenyls in sediments and soil along the Erh-Jen River”, Master Thesis, Tainan, Taiwan., National Cheng Kung university, 1991 28. Chen, I. M., Chang F.C. and Wang Y.S,” Reductive dechlorination of Hexachlorobenzene and Polychlorinated biphenyls in anaerobic sediments from tropical rivers with enrichment”, Proceeding of the Symposium on Chlorinated Dioxins and Related Compounds, 17th, 33, 189., Indianapolis,Indiana, 1997. 29. 陳健民著，環境毒物學，初版，台北縣中和市，新文京開發出版社有限公司 315-355，2002 30. 陳意銘，馴化厭氧菌對六氯苯還原性脫氯作用影響因子之探討，碩士論文，私立東吳大學微生物學研究所，1994。 31. C. Lamglolis, and H. Sloterdijk,” Contamination of Lake Saint-Pierre(St. Lawrence River) by organic and inorganic pollutants”, Rev. Sic. Eau, 2: 659-670, 1989. 32. Brahushi F., Dorfler, U.,Schroll, R., Munch, J.C,” Stimulatiom of reductive dechlorination of hexachlorobenzene in soil by inducing the native microbial activity”, Chemosphere, 55: 1477-1484, 2004 33. 盧志人，地下水的整治污染，國立編譯館，1998。 34. Suthan S. Suthersan,” Remediation engineering desing concepts” CRC Press, Inc, 1996. 35. Peikang Jin and Sanjoy K,” Bhattacharya. Anaerobic removal of pentachlorophenol in presence of Zinc”, Journal of Environmental Engineering, Vol, 122: 590-598, 1996 36. Chen, I. M., Chang, F.C., and Wang, Y.S,” Correlation of gas chromatographic properties of chlorobenzenes and polychlorinated biphenyls with the occurrence of reductive dechlorination by untamed microorganisms”, Chemosphere, 45(2): 223-229, 2001. 37. Brown J.F., Wagner, R.E., Feng, H., Bedard, D. L., Brennan, M. J.,Carnahan, J. 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Technol, 25：1082-1087, 1991 https://ir.cnu.edu.tw/handle/310902800/9094 https://ir.cnu.edu.tw/bitstream/310902800/9094/3/index.html 泰國運河脫氯作用多氯聯苯六氯苯 Thai Canal dechlorintion hexachlorobenzene polychlorinated biphenyls thesis 2006 ftchiananuniv 2022-05-15T05:23:21Z 本研究在測試台灣與泰國底泥環境之原生菌種的脫氯能力，在台灣地區採集二仁溪及後勁溪兩條河川之底泥進行六氯苯(Hexachlorobenzene, HCB)脫氯測試，在各種培養條件下其結果均顯示遲滯期甚長(>120天)，且無法將HCB完全的降解，只有在直接添加HCB粉未的這組實驗中有較短的遲滯期(30及90天)，但仍無法將HCB完全脫氯降解，而在多氯聯苯的實驗中，經120天的培養也都沒有脫氯作用發生，另外在泰國運河的底泥中進行HCB分析，結果顯示有4個點發現HCB和1.3.5-三氯苯(1.3.5-trichlorobenzene, 1.3.5-TCB)，因為1.3.5-TCB皆由高氯數之氯苯化合物降解而來，這顯示出泰國運河底泥微生物有自行脫氯能力，因此在泰國研究中則繼續以運河底泥中的厭氧微生物來進行HCB的還原脫氯作用，實驗分兩個階段，第一階段是由10點取得底泥微生物並應用於脫氯實驗上，過程中使用底泥加河水之底泥水再添加5g/L的yeast extract (YE)或是不添加。所有點中之HCB在14天後開始脫氯， 28-42天完全脫氯，未添加YE部份則在14至28天後有較緩慢的脫氯活動。事先添加養份和厭氧處理之組別在7天後，運河底泥微生物即有HCB之脫氯能力，並在6星期內完全脫氯，第二階段為評估HCB在經前處理及未處理的新鮮底泥水中之脫氯作用，結果顯示，底泥與水比例越高的組別其脫氯能力越強，底泥水不論過濾與否，都有HCB脫氯作用發生，另外，底泥水經前厭氧處理的時間越長，則對HCB的脫氯能力越佳。本研究結果顯示HCB脫氯菌廣闊的分佈在泰國運河沿岸的底泥，而且也顯示HCB污染的運河底泥之中有環境復育的可行性。 In order to investigate the hexachlorobenzene (HCB) dechlorintion ability of indigenous microbes in Taiwan and Thailand, river and canal sediments were collected and introduced in the HCB dechlorination tests. Most sets by using anaerobic microbes from Er-Jen River and Ho-Tsin River slowly dechlorinated HCB with a longer lag phase more than 120 days, and only those sets that added powder-like HCB showed short lag phase. Futhermore, these microbes cannot dechlorinate polychlorinated biphenyls within 120 days of incubation. In contrast to the weak HCB dechlorination ability of Taiwan river sediment anaerobes, indigenous microbes from Thai canal sediments showed more active dechlorination. The reason for Thai canal bacteria possessing novel HCB dechlorination potential might because the Canal was found contaminated by HCB. The HCB dechlorination experiments by Thai Canal microbes included two stages. In the first stage, sediments from 10 different sites were collected and the HCB dechlorination ability of the sediment microbes was also tested. In the sets with yeast extracts (YE), all 10 sites of microbes showed dechlorination activity after 14 days of incubation and completed the dechlorination within 28 ~ 42 days. In the sets without YE, the lag phase was about 14 ~ 28 days. In the second stage, HCB ... Thesis Arctic Chia Nan University of Pharmacy & Science Institutional Repository (CHNAIR)

河川底泥中六氯苯污染之環境復育研究

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