Exploring the utility of three nuclear regions to reconstruct reticulate evolution in the fern genus Asplenium
Three nuclear regions nuclear ribosomal internal transcribed spacer (nrITS) and intron regions of two nuclear low-copy genesgapCp, and pgiC, in combination with one chloroplast genome region were employed to explore patterns of reticulate evolution in the fern genus Asplenium. This is the one of the...
Published in: | Journal of Systematics and Evolution |
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WILEY
2013
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Online Access: | http://ir.ibcas.ac.cn/handle/2S10CLM1/28140 https://doi.org/10.1111/j.1759-6831.2012.00226.x |
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ftchiacadscibcas:oai:ir.ibcas.ac.cn:2S10CLM1/28140 2023-06-18T03:39:47+02:00 Exploring the utility of three nuclear regions to reconstruct reticulate evolution in the fern genus Asplenium Schneider, Harald Navarro-Gomez, Adriana Russell, Stephen J. Ansell, Stephen Grundmann, Michal Vogel, Johannes 2013 http://ir.ibcas.ac.cn/handle/2S10CLM1/28140 https://doi.org/10.1111/j.1759-6831.2012.00226.x 英语 eng WILEY JOURNAL OF SYSTEMATICS AND EVOLUTION http://ir.ibcas.ac.cn/handle/2S10CLM1/28140 doi:10.1111/j.1759-6831.2012.00226.x cn.org.cspace.api.content.CopyrightPolicy@162689bc ferns gapCp GAPDH hybridization introgression nrITS nuclear genes pgiC reticulate evolution Plant Sciences NEW-ZEALAND ASPLENIUM CHLOROPLAST DNA PHYLOGENETIC-RELATIONSHIPS RIBOSOMAL DNA SEQUENCES COMPLEX CETERACH ORIGIN RBCL PHYLOGEOGRAPHY Science Citation Index Expanded (SCI-EXPANDED) Article 期刊论文 2013 ftchiacadscibcas https://doi.org/10.1111/j.1759-6831.2012.00226.x 2023-06-07T12:28:39Z Three nuclear regions nuclear ribosomal internal transcribed spacer (nrITS) and intron regions of two nuclear low-copy genesgapCp, and pgiC, in combination with one chloroplast genome region were employed to explore patterns of reticulate evolution in the fern genus Asplenium. This is the one of the first studies using DNA sequences of multiple nuclear markers in ferns. All three nuclear markers amplified well with PCR and several copies were recovered by cloning PCR products. All three nuclear regions showed congruent results by recovering the neo-allotetraploid Asplenium adulterinum as the hybrid of diploid A. trichomanes and diploid A. viride. Conflicting results were obtained for several nodes. First, gapCp did not discriminate between A. aethiopicum and A. praegracile whereas the other markers recovered these two taxa as distinct. Conflicts among gene-trees were found in respect to A. monanthes, chloroplast and pgiC suggested a sister relationship of A. monanthes and A. trichomanes but gapCp and nrITS nested A. monanthes within A. normale. Our results confirm: (i) the usefulness of several nuclear regions, in particular gapCp and pgiC, to unravel reticulate evolution in ferns and species differentiation and (ii) highlights the need to employ more than one nuclear region to obtain reliable hypotheses on reticulate events versus incomplete lineage sorting. Especially, if one assumes that the reticulation event might have occurred in the more distant past. Considering the expected high frequency of reticulate evolution in ferns, the establishment of robust and informative nuclear genomic markers is critical to achieve further progress in our efforts to elucidate fern evolution. Article in Journal/Newspaper Asplenium adulterinum Institute of Botany: IBCAS OpenIR (Chinese Academy Of Sciences) New Zealand Journal of Systematics and Evolution 51 2 142 153 |
institution |
Open Polar |
collection |
Institute of Botany: IBCAS OpenIR (Chinese Academy Of Sciences) |
op_collection_id |
ftchiacadscibcas |
language |
English |
topic |
ferns gapCp GAPDH hybridization introgression nrITS nuclear genes pgiC reticulate evolution Plant Sciences NEW-ZEALAND ASPLENIUM CHLOROPLAST DNA PHYLOGENETIC-RELATIONSHIPS RIBOSOMAL DNA SEQUENCES COMPLEX CETERACH ORIGIN RBCL PHYLOGEOGRAPHY Science Citation Index Expanded (SCI-EXPANDED) |
spellingShingle |
ferns gapCp GAPDH hybridization introgression nrITS nuclear genes pgiC reticulate evolution Plant Sciences NEW-ZEALAND ASPLENIUM CHLOROPLAST DNA PHYLOGENETIC-RELATIONSHIPS RIBOSOMAL DNA SEQUENCES COMPLEX CETERACH ORIGIN RBCL PHYLOGEOGRAPHY Science Citation Index Expanded (SCI-EXPANDED) Schneider, Harald Navarro-Gomez, Adriana Russell, Stephen J. Ansell, Stephen Grundmann, Michal Vogel, Johannes Exploring the utility of three nuclear regions to reconstruct reticulate evolution in the fern genus Asplenium |
topic_facet |
ferns gapCp GAPDH hybridization introgression nrITS nuclear genes pgiC reticulate evolution Plant Sciences NEW-ZEALAND ASPLENIUM CHLOROPLAST DNA PHYLOGENETIC-RELATIONSHIPS RIBOSOMAL DNA SEQUENCES COMPLEX CETERACH ORIGIN RBCL PHYLOGEOGRAPHY Science Citation Index Expanded (SCI-EXPANDED) |
description |
Three nuclear regions nuclear ribosomal internal transcribed spacer (nrITS) and intron regions of two nuclear low-copy genesgapCp, and pgiC, in combination with one chloroplast genome region were employed to explore patterns of reticulate evolution in the fern genus Asplenium. This is the one of the first studies using DNA sequences of multiple nuclear markers in ferns. All three nuclear markers amplified well with PCR and several copies were recovered by cloning PCR products. All three nuclear regions showed congruent results by recovering the neo-allotetraploid Asplenium adulterinum as the hybrid of diploid A. trichomanes and diploid A. viride. Conflicting results were obtained for several nodes. First, gapCp did not discriminate between A. aethiopicum and A. praegracile whereas the other markers recovered these two taxa as distinct. Conflicts among gene-trees were found in respect to A. monanthes, chloroplast and pgiC suggested a sister relationship of A. monanthes and A. trichomanes but gapCp and nrITS nested A. monanthes within A. normale. Our results confirm: (i) the usefulness of several nuclear regions, in particular gapCp and pgiC, to unravel reticulate evolution in ferns and species differentiation and (ii) highlights the need to employ more than one nuclear region to obtain reliable hypotheses on reticulate events versus incomplete lineage sorting. Especially, if one assumes that the reticulation event might have occurred in the more distant past. Considering the expected high frequency of reticulate evolution in ferns, the establishment of robust and informative nuclear genomic markers is critical to achieve further progress in our efforts to elucidate fern evolution. |
format |
Article in Journal/Newspaper |
author |
Schneider, Harald Navarro-Gomez, Adriana Russell, Stephen J. Ansell, Stephen Grundmann, Michal Vogel, Johannes |
author_facet |
Schneider, Harald Navarro-Gomez, Adriana Russell, Stephen J. Ansell, Stephen Grundmann, Michal Vogel, Johannes |
author_sort |
Schneider, Harald |
title |
Exploring the utility of three nuclear regions to reconstruct reticulate evolution in the fern genus Asplenium |
title_short |
Exploring the utility of three nuclear regions to reconstruct reticulate evolution in the fern genus Asplenium |
title_full |
Exploring the utility of three nuclear regions to reconstruct reticulate evolution in the fern genus Asplenium |
title_fullStr |
Exploring the utility of three nuclear regions to reconstruct reticulate evolution in the fern genus Asplenium |
title_full_unstemmed |
Exploring the utility of three nuclear regions to reconstruct reticulate evolution in the fern genus Asplenium |
title_sort |
exploring the utility of three nuclear regions to reconstruct reticulate evolution in the fern genus asplenium |
publisher |
WILEY |
publishDate |
2013 |
url |
http://ir.ibcas.ac.cn/handle/2S10CLM1/28140 https://doi.org/10.1111/j.1759-6831.2012.00226.x |
geographic |
New Zealand |
geographic_facet |
New Zealand |
genre |
Asplenium adulterinum |
genre_facet |
Asplenium adulterinum |
op_relation |
JOURNAL OF SYSTEMATICS AND EVOLUTION http://ir.ibcas.ac.cn/handle/2S10CLM1/28140 doi:10.1111/j.1759-6831.2012.00226.x |
op_rights |
cn.org.cspace.api.content.CopyrightPolicy@162689bc |
op_doi |
https://doi.org/10.1111/j.1759-6831.2012.00226.x |
container_title |
Journal of Systematics and Evolution |
container_volume |
51 |
container_issue |
2 |
container_start_page |
142 |
op_container_end_page |
153 |
_version_ |
1769004573520822272 |