Bioactivity of Cod and Chicken Protein Hydrolysates before and after in vitro Gastrointestinal Digestion
Bioactivity of cod (Gadus morhua) and chicken (Gallus domesticus) protein hydrolysates before and aft er in vitro gastrointestinal (GI) digestion was investigated using yeast Saccharomyces cerevisiae as a model organism. Both hydrolysates were exposed to in vitro GI digestion prior to cellular expos...
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ftchalmersuniv:oai:research.chalmers.se:252556 2023-06-11T04:11:48+02:00 Bioactivity of Cod and Chicken Protein Hydrolysates before and after in vitro Gastrointestinal Digestion Jamnik, P. Istenic, K. Kostomaj, T. Wulff, T. Geirsdottir, M. Almgren, Annette Jonsdottir, R. Kristinsson, H. G. Undeland, Ingrid 2017 text https://doi.org/10.17113/ftb.55.03.17.5117 https://research.chalmers.se/en/publication/252556 unknown http://dx.doi.org/10.17113/ftb.55.03.17.5117 https://research.chalmers.se/en/publication/252556 Biochemistry and Molecular Biology cod protein hydrolysates proteomics chicken in vitro gastrointestinal digestion yeast 2017 ftchalmersuniv https://doi.org/10.17113/ftb.55.03.17.5117 2023-05-31T22:35:38Z Bioactivity of cod (Gadus morhua) and chicken (Gallus domesticus) protein hydrolysates before and aft er in vitro gastrointestinal (GI) digestion was investigated using yeast Saccharomyces cerevisiae as a model organism. Both hydrolysates were exposed to in vitro GI digestion prior to cellular exposure to simulate digestion conditions in the human body and therefore investigate the role of modulations in the GI tract on the cell response. The effect of digested and undigested hydrolysates on intracellular oxidation, cellular metabolic energy and proteome level was investigated. No diff erence in the eff ect on intracellular oxidation activity was obtained between cod and chicken hydrolysates, while higher aff ect on intracellular oxidation was provided by digested hydrolysates, with relative values of intracellular oxidation of cod of (70.2 +/- 0.8) and chicken of (74.5 +/- 1.4) % than by undigested ones, where values of cod and chicken were (95.5 +/- 1.2) and (90.5 +/- 0.7) %, respectively. Neither species nor digestion had any eff ect on cellular metabolic energy. At proteome level, digested hydrolysates gave again signifi cantly stronger responses than undigested counterparts; cod peptides here also gave somewhat stronger response than chicken peptides. The knowledge of the action of food protein hydrolysates and their digests within live cells, also at proteome level, is important for further validation of their activity in higher eukaryotes to develop new functional food ingredients, such as in this case chicken and cod muscle-derived peptides. Other/Unknown Material Gadus morhua Chalmers University of Technology: Chalmers research Food Technology and Biotechnology 55 3 |
institution |
Open Polar |
collection |
Chalmers University of Technology: Chalmers research |
op_collection_id |
ftchalmersuniv |
language |
unknown |
topic |
Biochemistry and Molecular Biology cod protein hydrolysates proteomics chicken in vitro gastrointestinal digestion yeast |
spellingShingle |
Biochemistry and Molecular Biology cod protein hydrolysates proteomics chicken in vitro gastrointestinal digestion yeast Jamnik, P. Istenic, K. Kostomaj, T. Wulff, T. Geirsdottir, M. Almgren, Annette Jonsdottir, R. Kristinsson, H. G. Undeland, Ingrid Bioactivity of Cod and Chicken Protein Hydrolysates before and after in vitro Gastrointestinal Digestion |
topic_facet |
Biochemistry and Molecular Biology cod protein hydrolysates proteomics chicken in vitro gastrointestinal digestion yeast |
description |
Bioactivity of cod (Gadus morhua) and chicken (Gallus domesticus) protein hydrolysates before and aft er in vitro gastrointestinal (GI) digestion was investigated using yeast Saccharomyces cerevisiae as a model organism. Both hydrolysates were exposed to in vitro GI digestion prior to cellular exposure to simulate digestion conditions in the human body and therefore investigate the role of modulations in the GI tract on the cell response. The effect of digested and undigested hydrolysates on intracellular oxidation, cellular metabolic energy and proteome level was investigated. No diff erence in the eff ect on intracellular oxidation activity was obtained between cod and chicken hydrolysates, while higher aff ect on intracellular oxidation was provided by digested hydrolysates, with relative values of intracellular oxidation of cod of (70.2 +/- 0.8) and chicken of (74.5 +/- 1.4) % than by undigested ones, where values of cod and chicken were (95.5 +/- 1.2) and (90.5 +/- 0.7) %, respectively. Neither species nor digestion had any eff ect on cellular metabolic energy. At proteome level, digested hydrolysates gave again signifi cantly stronger responses than undigested counterparts; cod peptides here also gave somewhat stronger response than chicken peptides. The knowledge of the action of food protein hydrolysates and their digests within live cells, also at proteome level, is important for further validation of their activity in higher eukaryotes to develop new functional food ingredients, such as in this case chicken and cod muscle-derived peptides. |
author |
Jamnik, P. Istenic, K. Kostomaj, T. Wulff, T. Geirsdottir, M. Almgren, Annette Jonsdottir, R. Kristinsson, H. G. Undeland, Ingrid |
author_facet |
Jamnik, P. Istenic, K. Kostomaj, T. Wulff, T. Geirsdottir, M. Almgren, Annette Jonsdottir, R. Kristinsson, H. G. Undeland, Ingrid |
author_sort |
Jamnik, P. |
title |
Bioactivity of Cod and Chicken Protein Hydrolysates before and after in vitro Gastrointestinal Digestion |
title_short |
Bioactivity of Cod and Chicken Protein Hydrolysates before and after in vitro Gastrointestinal Digestion |
title_full |
Bioactivity of Cod and Chicken Protein Hydrolysates before and after in vitro Gastrointestinal Digestion |
title_fullStr |
Bioactivity of Cod and Chicken Protein Hydrolysates before and after in vitro Gastrointestinal Digestion |
title_full_unstemmed |
Bioactivity of Cod and Chicken Protein Hydrolysates before and after in vitro Gastrointestinal Digestion |
title_sort |
bioactivity of cod and chicken protein hydrolysates before and after in vitro gastrointestinal digestion |
publishDate |
2017 |
url |
https://doi.org/10.17113/ftb.55.03.17.5117 https://research.chalmers.se/en/publication/252556 |
genre |
Gadus morhua |
genre_facet |
Gadus morhua |
op_relation |
http://dx.doi.org/10.17113/ftb.55.03.17.5117 https://research.chalmers.se/en/publication/252556 |
op_doi |
https://doi.org/10.17113/ftb.55.03.17.5117 |
container_title |
Food Technology and Biotechnology |
container_volume |
55 |
container_issue |
3 |
_version_ |
1768387119470870528 |