Probing Amyloid-β Pathology in transgenic Alzheimer's disease (tgArcSwe) mice using MALDI Imaging Mass Spectrometry
The pathological mechanisms underlying Alzheimer's disease (AD) are still not understood. The disease pathology is characterized by accumulation and aggregation of amyloid-β (Aβ) peptides into extracellular plaques, however the factors that promote neurotoxic Aβ aggregation remain elusive. Imag...
Published in: | Journal of Neurochemistry |
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Online Access: | https://doi.org/10.1111/jnc.13645 https://research.chalmers.se/en/publication/235423 |
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ftchalmersuniv:oai:research.chalmers.se:235423 2023-05-15T15:10:15+02:00 Probing Amyloid-β Pathology in transgenic Alzheimer's disease (tgArcSwe) mice using MALDI Imaging Mass Spectrometry Carlred, Louise M Michno, W. Kaya, I. Sjövall, Peter Syvanen, S. Hanrieder, Jörg 2016 text https://doi.org/10.1111/jnc.13645 https://research.chalmers.se/en/publication/235423 unknown http://dx.doi.org/10.1111/jnc.13645 https://research.chalmers.se/en/publication/235423 Biochemistry and Molecular Biology Analytical Chemistry Neurosciences Cell and Molecular Biology Alzheimer's disease MALDI imaging amyloid beta dementia 2016 ftchalmersuniv https://doi.org/10.1111/jnc.13645 2022-12-11T07:05:50Z The pathological mechanisms underlying Alzheimer's disease (AD) are still not understood. The disease pathology is characterized by accumulation and aggregation of amyloid-β (Aβ) peptides into extracellular plaques, however the factors that promote neurotoxic Aβ aggregation remain elusive. Imaging mass spectrometry (IMS) is a powerful technique to comprehensively elucidate the spatial distribution patterns of lipids, peptides and proteins in biological tissues. In the present study, matrix-assisted laser desorption/ionization (MALDI) mass spectrometry (MS) based imaging was used to study Aβ deposition in transgenic mouse brain tissue and to elucidate the plaque associated chemical microenvironment. The imaging experiments were performed in brain sections of transgenic Alzheimer's disease mice carrying the Arctic and Swedish mutation of amyloid-beta precursor protein (tgArcSwe). Multivariate image analysis was used to interrogate the IMS data for identifying pathologically relevant, anatomical features based on their chemical identity. This include cortical and hippocampal Aβ deposits, whose amyloid peptide content was further verified using immunohistochemistry and laser micro dissection followed by MALDI MS analysis. Subsequent statistical analysis on spectral data of regions of interest (ROI) revealed brain region specific differences in Aβ peptide aggregation. Moreover, other plaque associated protein species were identified including macrophage migration inhibitory factor (MIF) suggesting neuroinflammatory processes and glial cell reactivity to be involved in AD pathology. The presented data further highlight the potential of IMS as powerful approach in neuropathology. Other/Unknown Material Arctic Chalmers University of Technology: Chalmers research Arctic Journal of Neurochemistry 138 3 469 478 |
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Chalmers University of Technology: Chalmers research |
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Biochemistry and Molecular Biology Analytical Chemistry Neurosciences Cell and Molecular Biology Alzheimer's disease MALDI imaging amyloid beta dementia |
spellingShingle |
Biochemistry and Molecular Biology Analytical Chemistry Neurosciences Cell and Molecular Biology Alzheimer's disease MALDI imaging amyloid beta dementia Carlred, Louise M Michno, W. Kaya, I. Sjövall, Peter Syvanen, S. Hanrieder, Jörg Probing Amyloid-β Pathology in transgenic Alzheimer's disease (tgArcSwe) mice using MALDI Imaging Mass Spectrometry |
topic_facet |
Biochemistry and Molecular Biology Analytical Chemistry Neurosciences Cell and Molecular Biology Alzheimer's disease MALDI imaging amyloid beta dementia |
description |
The pathological mechanisms underlying Alzheimer's disease (AD) are still not understood. The disease pathology is characterized by accumulation and aggregation of amyloid-β (Aβ) peptides into extracellular plaques, however the factors that promote neurotoxic Aβ aggregation remain elusive. Imaging mass spectrometry (IMS) is a powerful technique to comprehensively elucidate the spatial distribution patterns of lipids, peptides and proteins in biological tissues. In the present study, matrix-assisted laser desorption/ionization (MALDI) mass spectrometry (MS) based imaging was used to study Aβ deposition in transgenic mouse brain tissue and to elucidate the plaque associated chemical microenvironment. The imaging experiments were performed in brain sections of transgenic Alzheimer's disease mice carrying the Arctic and Swedish mutation of amyloid-beta precursor protein (tgArcSwe). Multivariate image analysis was used to interrogate the IMS data for identifying pathologically relevant, anatomical features based on their chemical identity. This include cortical and hippocampal Aβ deposits, whose amyloid peptide content was further verified using immunohistochemistry and laser micro dissection followed by MALDI MS analysis. Subsequent statistical analysis on spectral data of regions of interest (ROI) revealed brain region specific differences in Aβ peptide aggregation. Moreover, other plaque associated protein species were identified including macrophage migration inhibitory factor (MIF) suggesting neuroinflammatory processes and glial cell reactivity to be involved in AD pathology. The presented data further highlight the potential of IMS as powerful approach in neuropathology. |
author |
Carlred, Louise M Michno, W. Kaya, I. Sjövall, Peter Syvanen, S. Hanrieder, Jörg |
author_facet |
Carlred, Louise M Michno, W. Kaya, I. Sjövall, Peter Syvanen, S. Hanrieder, Jörg |
author_sort |
Carlred, Louise M |
title |
Probing Amyloid-β Pathology in transgenic Alzheimer's disease (tgArcSwe) mice using MALDI Imaging Mass Spectrometry |
title_short |
Probing Amyloid-β Pathology in transgenic Alzheimer's disease (tgArcSwe) mice using MALDI Imaging Mass Spectrometry |
title_full |
Probing Amyloid-β Pathology in transgenic Alzheimer's disease (tgArcSwe) mice using MALDI Imaging Mass Spectrometry |
title_fullStr |
Probing Amyloid-β Pathology in transgenic Alzheimer's disease (tgArcSwe) mice using MALDI Imaging Mass Spectrometry |
title_full_unstemmed |
Probing Amyloid-β Pathology in transgenic Alzheimer's disease (tgArcSwe) mice using MALDI Imaging Mass Spectrometry |
title_sort |
probing amyloid-β pathology in transgenic alzheimer's disease (tgarcswe) mice using maldi imaging mass spectrometry |
publishDate |
2016 |
url |
https://doi.org/10.1111/jnc.13645 https://research.chalmers.se/en/publication/235423 |
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Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
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Arctic |
op_relation |
http://dx.doi.org/10.1111/jnc.13645 https://research.chalmers.se/en/publication/235423 |
op_doi |
https://doi.org/10.1111/jnc.13645 |
container_title |
Journal of Neurochemistry |
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138 |
container_issue |
3 |
container_start_page |
469 |
op_container_end_page |
478 |
_version_ |
1766341302207643648 |