Nitrifiers Cooperate to Produce Nitrous Oxide in Plateau Wetland Sediments
The thawing of dormant plateau permafrost emits nitrous oxide (N2O) through wetlands; however, the N2O production mechanism in plateau wetlands is still unclear. Here, we used the 15N-18O double tracer technique and metagenomic sequencing to analyze the N2O production mechanism in the Yunnan-Kweicho...
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ftchacadscircees:oai:/ir.rcees.ac.cn:311016/48568 2023-06-11T04:15:57+02:00 Nitrifiers Cooperate to Produce Nitrous Oxide in Plateau Wetland Sediments Yuan, Dongdan Zheng, Lei Liu, Yong-Xin Cheng, Hongguang Ding, Aizhong Wang, Xiaomin Tan, Qiuyang Wang, Xue Xing, Yuzi Xie, En Wu, Haoming Wang, Shanyun Zhu, Guibing 2022 https://ir.rcees.ac.cn/handle/311016/48568 unknown ENVIRONMENTAL SCIENCE & TECHNOLOGY https://ir.rcees.ac.cn/handle/311016/48568 cn.org.cspace.api.content.CopyrightPolicy@728a71fe plateau wetland sediments nitrous oxide nitrifier denitrification complete ammonia oxidizing bacteria ammonia oxidizing bacteria cooperation pattern 期刊论文 2022 ftchacadscircees 2023-05-28T12:17:54Z The thawing of dormant plateau permafrost emits nitrous oxide (N2O) through wetlands; however, the N2O production mechanism in plateau wetlands is still unclear. Here, we used the 15N-18O double tracer technique and metagenomic sequencing to analyze the N2O production mechanism in the Yunnan-Kweichow and Qinghai-Tibet plateau wetlands during the summer of 2020. N2O production activity was detected in all 16 sediment samples (elevation 1020-4601 m: 2.55 +/- 0.42-26.38 +/- 3.25 ng N g-1 d-1) and was promoted by nitrifier denitrification (ND). The key functional genes of ND (amoA, hao, and nirK) belonged to complete ammonia oxidizing (comammox) bacteria, and the key ND species was the comammox bacterium Nitrospira nitrificans. We found that the comammox bacterial species N. nitrificans and the ammonia oxidizing bacterial (AOB) species Nitrosomonas europaea cooperate to produce N2O in the plateau wetland sediments. Furthermore, we inferred that environmental factors (elevation and total organic matter (TOM)) influence the cooperation pattern via N. nitrificans, thus affecting the N2O production activity in the plateau wetland sediments. Our findings advance the mechanistic understanding of nitrifiers in biogeochemical cycles and global climate change. Report permafrost Research Center for Eco-Environmental Sciences: RCEES OpenIR (Chinese Academy of Sciences) |
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Open Polar |
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Research Center for Eco-Environmental Sciences: RCEES OpenIR (Chinese Academy of Sciences) |
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ftchacadscircees |
language |
unknown |
topic |
plateau wetland sediments nitrous oxide nitrifier denitrification complete ammonia oxidizing bacteria ammonia oxidizing bacteria cooperation pattern |
spellingShingle |
plateau wetland sediments nitrous oxide nitrifier denitrification complete ammonia oxidizing bacteria ammonia oxidizing bacteria cooperation pattern Yuan, Dongdan Zheng, Lei Liu, Yong-Xin Cheng, Hongguang Ding, Aizhong Wang, Xiaomin Tan, Qiuyang Wang, Xue Xing, Yuzi Xie, En Wu, Haoming Wang, Shanyun Zhu, Guibing Nitrifiers Cooperate to Produce Nitrous Oxide in Plateau Wetland Sediments |
topic_facet |
plateau wetland sediments nitrous oxide nitrifier denitrification complete ammonia oxidizing bacteria ammonia oxidizing bacteria cooperation pattern |
description |
The thawing of dormant plateau permafrost emits nitrous oxide (N2O) through wetlands; however, the N2O production mechanism in plateau wetlands is still unclear. Here, we used the 15N-18O double tracer technique and metagenomic sequencing to analyze the N2O production mechanism in the Yunnan-Kweichow and Qinghai-Tibet plateau wetlands during the summer of 2020. N2O production activity was detected in all 16 sediment samples (elevation 1020-4601 m: 2.55 +/- 0.42-26.38 +/- 3.25 ng N g-1 d-1) and was promoted by nitrifier denitrification (ND). The key functional genes of ND (amoA, hao, and nirK) belonged to complete ammonia oxidizing (comammox) bacteria, and the key ND species was the comammox bacterium Nitrospira nitrificans. We found that the comammox bacterial species N. nitrificans and the ammonia oxidizing bacterial (AOB) species Nitrosomonas europaea cooperate to produce N2O in the plateau wetland sediments. Furthermore, we inferred that environmental factors (elevation and total organic matter (TOM)) influence the cooperation pattern via N. nitrificans, thus affecting the N2O production activity in the plateau wetland sediments. Our findings advance the mechanistic understanding of nitrifiers in biogeochemical cycles and global climate change. |
format |
Report |
author |
Yuan, Dongdan Zheng, Lei Liu, Yong-Xin Cheng, Hongguang Ding, Aizhong Wang, Xiaomin Tan, Qiuyang Wang, Xue Xing, Yuzi Xie, En Wu, Haoming Wang, Shanyun Zhu, Guibing |
author_facet |
Yuan, Dongdan Zheng, Lei Liu, Yong-Xin Cheng, Hongguang Ding, Aizhong Wang, Xiaomin Tan, Qiuyang Wang, Xue Xing, Yuzi Xie, En Wu, Haoming Wang, Shanyun Zhu, Guibing |
author_sort |
Yuan, Dongdan |
title |
Nitrifiers Cooperate to Produce Nitrous Oxide in Plateau Wetland Sediments |
title_short |
Nitrifiers Cooperate to Produce Nitrous Oxide in Plateau Wetland Sediments |
title_full |
Nitrifiers Cooperate to Produce Nitrous Oxide in Plateau Wetland Sediments |
title_fullStr |
Nitrifiers Cooperate to Produce Nitrous Oxide in Plateau Wetland Sediments |
title_full_unstemmed |
Nitrifiers Cooperate to Produce Nitrous Oxide in Plateau Wetland Sediments |
title_sort |
nitrifiers cooperate to produce nitrous oxide in plateau wetland sediments |
publishDate |
2022 |
url |
https://ir.rcees.ac.cn/handle/311016/48568 |
genre |
permafrost |
genre_facet |
permafrost |
op_relation |
ENVIRONMENTAL SCIENCE & TECHNOLOGY https://ir.rcees.ac.cn/handle/311016/48568 |
op_rights |
cn.org.cspace.api.content.CopyrightPolicy@728a71fe |
_version_ |
1768373235628376064 |