The effect of enzyme loading, alcohol/acid ratio and temperature on the enzymatic esterification of levulinic acid with methanol for methyl levulinate production: a kinetic study
As an important bio-based chemical, methyl levulinate (ML) can be produced via enzymatic esterification of levulinic acid with methanol. A kinetic model is developed in this work based on the law of mass action and reaction reversibility, to investigate the effect of enzyme loading, alcohol/acid rat...
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ROYAL SOC CHEMISTRY
2021
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ftchacadsciegiec:oai:ir.giec.ac.cn:344007/33162 2023-05-15T14:01:45+02:00 The effect of enzyme loading, alcohol/acid ratio and temperature on the enzymatic esterification of levulinic acid with methanol for methyl levulinate production: a kinetic study Song, Miaojia Di, Xiaohui Zhang, Yu Sun, Yongming Wang, Zhongming Yuan, Zhenhong Guo, Ying 2021-05-04 http://ir.giec.ac.cn/handle/344007/33162 https://doi.org/10.1039/d1ra01780b 英语 eng ROYAL SOC CHEMISTRY RSC ADVANCES http://ir.giec.ac.cn/handle/344007/33162 doi:10.1039/d1ra01780b LIPASE-CATALYZED ESTERIFICATION CANDIDA-ANTARCTICA IMMOBILIZATION RESOLUTION Chemistry Multidisciplinary 期刊论文 2021 ftchacadsciegiec https://doi.org/10.1039/d1ra01780b 2022-09-23T14:17:59Z As an important bio-based chemical, methyl levulinate (ML) can be produced via enzymatic esterification of levulinic acid with methanol. A kinetic model is developed in this work based on the law of mass action and reaction reversibility, to investigate the effect of enzyme loading, alcohol/acid ratio and temperature on ML yield. Data analysis shows that newly developed binary regression is apparently more persuasive than the commonly used unitary regression. Kinetic study reveals: (1) rate constants of esterification/hydrolysis increase with increasing enzyme loading, while their ratio (equilibrium constant) remains invariant. (2) Methanol has no toxicity towards lipase, and hence, neither the rate constants of esterification/hydrolysis nor the equilibrium constant are affected by alcohol/acid ratio. (3) Both rate constants of esterification/hydrolysis and the equilibrium constant increase with temperature elevation, and their relationships agree with Arrhenius equation and Van't Hoff equation, respectively. (4) The esterification is endothermic and spontaneous. In total, the application of binary regression analysis for the developed model to study the enzymatic esterification kinetics is quite successful. Report Antarc* Antarctica Guangzhou Institute of Energy Conversion, Chinese Academy of Sciences: GIEC OpenIR RSC Advances 11 25 15054 15059 |
institution |
Open Polar |
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Guangzhou Institute of Energy Conversion, Chinese Academy of Sciences: GIEC OpenIR |
op_collection_id |
ftchacadsciegiec |
language |
English |
topic |
LIPASE-CATALYZED ESTERIFICATION CANDIDA-ANTARCTICA IMMOBILIZATION RESOLUTION Chemistry Multidisciplinary |
spellingShingle |
LIPASE-CATALYZED ESTERIFICATION CANDIDA-ANTARCTICA IMMOBILIZATION RESOLUTION Chemistry Multidisciplinary Song, Miaojia Di, Xiaohui Zhang, Yu Sun, Yongming Wang, Zhongming Yuan, Zhenhong Guo, Ying The effect of enzyme loading, alcohol/acid ratio and temperature on the enzymatic esterification of levulinic acid with methanol for methyl levulinate production: a kinetic study |
topic_facet |
LIPASE-CATALYZED ESTERIFICATION CANDIDA-ANTARCTICA IMMOBILIZATION RESOLUTION Chemistry Multidisciplinary |
description |
As an important bio-based chemical, methyl levulinate (ML) can be produced via enzymatic esterification of levulinic acid with methanol. A kinetic model is developed in this work based on the law of mass action and reaction reversibility, to investigate the effect of enzyme loading, alcohol/acid ratio and temperature on ML yield. Data analysis shows that newly developed binary regression is apparently more persuasive than the commonly used unitary regression. Kinetic study reveals: (1) rate constants of esterification/hydrolysis increase with increasing enzyme loading, while their ratio (equilibrium constant) remains invariant. (2) Methanol has no toxicity towards lipase, and hence, neither the rate constants of esterification/hydrolysis nor the equilibrium constant are affected by alcohol/acid ratio. (3) Both rate constants of esterification/hydrolysis and the equilibrium constant increase with temperature elevation, and their relationships agree with Arrhenius equation and Van't Hoff equation, respectively. (4) The esterification is endothermic and spontaneous. In total, the application of binary regression analysis for the developed model to study the enzymatic esterification kinetics is quite successful. |
format |
Report |
author |
Song, Miaojia Di, Xiaohui Zhang, Yu Sun, Yongming Wang, Zhongming Yuan, Zhenhong Guo, Ying |
author_facet |
Song, Miaojia Di, Xiaohui Zhang, Yu Sun, Yongming Wang, Zhongming Yuan, Zhenhong Guo, Ying |
author_sort |
Song, Miaojia |
title |
The effect of enzyme loading, alcohol/acid ratio and temperature on the enzymatic esterification of levulinic acid with methanol for methyl levulinate production: a kinetic study |
title_short |
The effect of enzyme loading, alcohol/acid ratio and temperature on the enzymatic esterification of levulinic acid with methanol for methyl levulinate production: a kinetic study |
title_full |
The effect of enzyme loading, alcohol/acid ratio and temperature on the enzymatic esterification of levulinic acid with methanol for methyl levulinate production: a kinetic study |
title_fullStr |
The effect of enzyme loading, alcohol/acid ratio and temperature on the enzymatic esterification of levulinic acid with methanol for methyl levulinate production: a kinetic study |
title_full_unstemmed |
The effect of enzyme loading, alcohol/acid ratio and temperature on the enzymatic esterification of levulinic acid with methanol for methyl levulinate production: a kinetic study |
title_sort |
effect of enzyme loading, alcohol/acid ratio and temperature on the enzymatic esterification of levulinic acid with methanol for methyl levulinate production: a kinetic study |
publisher |
ROYAL SOC CHEMISTRY |
publishDate |
2021 |
url |
http://ir.giec.ac.cn/handle/344007/33162 https://doi.org/10.1039/d1ra01780b |
genre |
Antarc* Antarctica |
genre_facet |
Antarc* Antarctica |
op_relation |
RSC ADVANCES http://ir.giec.ac.cn/handle/344007/33162 doi:10.1039/d1ra01780b |
op_doi |
https://doi.org/10.1039/d1ra01780b |
container_title |
RSC Advances |
container_volume |
11 |
container_issue |
25 |
container_start_page |
15054 |
op_container_end_page |
15059 |
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1766271792906764288 |