Comparison of the Biochemical Composition and Nutritional Quality Between Diploid and Triploid Hong Kong Oysters, Crassostrea hongkongensis
This study is the first systematic comparison of the biochemical composition and nutritional quality between diploid and triploid Hong Kong oysters, Crassostrea hongkongensis. Results showed that in the reproductive season, the glycogen content in five tissues (gill, mantle, adductor muscle, labial...
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Language: | English |
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FRONTIERS MEDIA SA
2018
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Online Access: | http://ir.gig.ac.cn/handle/344008/40439 https://doi.org/10.3389/fphys.2018.01674 |
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ftchacadscgigcas:oai:ir.gig.ac.cn:344008/40439 2023-05-15T17:54:21+02:00 Comparison of the Biochemical Composition and Nutritional Quality Between Diploid and Triploid Hong Kong Oysters, Crassostrea hongkongensis Qin, Yanping Zhang, Yuehuan Ma, Haitao Wu, Xiangwei Xiao, Shu Li, Jun Mo, Riguan Yu, Ziniu 2018-11-26 http://ir.gig.ac.cn/handle/344008/40439 https://doi.org/10.3389/fphys.2018.01674 英语 eng FRONTIERS MEDIA SA FRONTIERS IN PHYSIOLOGY http://ir.gig.ac.cn/handle/344008/40439 doi:10.3389/fphys.2018.01674 Physiology Crassostrea hongkongensis diploid triploid biochemical compositions nutritional quality metabolic pathways FATTY-ACID-COMPOSITION PACIFIC OYSTER GLYCOGEN-CONTENT REPRODUCTIVE-CYCLE SEASONAL-VARIATION TAPES-PHILIPPINARUM MOLECULAR-CLONING CYTOCHALASIN-B GIGAS THUNBERG FUJIAN OYSTER 期刊论文 2018 ftchacadscgigcas https://doi.org/10.3389/fphys.2018.01674 2020-12-22T07:21:37Z This study is the first systematic comparison of the biochemical composition and nutritional quality between diploid and triploid Hong Kong oysters, Crassostrea hongkongensis. Results showed that in the reproductive season, the glycogen content in five tissues (gill, mantle, adductor muscle, labial palps and gonad) was significantly higher (P < 0.05) in triploids than in diploids, with odds ratios (ORs) of 96.26, 60.17, 72.59, 53.56, and 128.52%, respectively. In the non-reproductive phase, significant differences in glycogen content (P < 0.05) between diploid and triploid oysters existed only in gill and gonad. In both diploid and triploid Hong Kong oysters, quantitative real-time PCR analysis of the glycogen synthesis gene (ChGS) and glycogen phosphorylase gene (ChGP) showed that the gene expression patterns matched the pattern of variation in glycogen content. Moreover, in both the reproductive and the non-reproductive phases, triploid Hong Kong oysters had a well balance of essential amino acids and were thus a well source of high-quality protein. Surprisingly, in both phases, significantly higher (P < 0.05) percentages of four essential fatty acids (a-linolenic acid, linoleic acid, eicosapentaenoic acid, and docosahexaenoic acid) were observed in triploids than in diploids. Additionally, the ratio of n-3/n-6 polyunsaturated fatty acids (PUFAs) was much higher in triploids than that in diploids. Variations in Biochemical composition were consistent with the relative expression of the citrate synthase gene (ChCS) and the a-ketoglutarate dehydrogenase gene (ChKD), which are key enzyme genes of the tricarboxylic acid cycle. Overall, the triploid Hong Kong oyster has a better nutritional value and taste than the diploid in terms of glycogen content, protein quality and fatty acid content. Report Pacific oyster Guangzhou Institute of Geochemistry: GIG OpenIR (Chinese Academy of Sciences) Pacific Frontiers in Physiology 9 |
institution |
Open Polar |
collection |
Guangzhou Institute of Geochemistry: GIG OpenIR (Chinese Academy of Sciences) |
op_collection_id |
ftchacadscgigcas |
language |
English |
topic |
Physiology Crassostrea hongkongensis diploid triploid biochemical compositions nutritional quality metabolic pathways FATTY-ACID-COMPOSITION PACIFIC OYSTER GLYCOGEN-CONTENT REPRODUCTIVE-CYCLE SEASONAL-VARIATION TAPES-PHILIPPINARUM MOLECULAR-CLONING CYTOCHALASIN-B GIGAS THUNBERG FUJIAN OYSTER |
spellingShingle |
Physiology Crassostrea hongkongensis diploid triploid biochemical compositions nutritional quality metabolic pathways FATTY-ACID-COMPOSITION PACIFIC OYSTER GLYCOGEN-CONTENT REPRODUCTIVE-CYCLE SEASONAL-VARIATION TAPES-PHILIPPINARUM MOLECULAR-CLONING CYTOCHALASIN-B GIGAS THUNBERG FUJIAN OYSTER Qin, Yanping Zhang, Yuehuan Ma, Haitao Wu, Xiangwei Xiao, Shu Li, Jun Mo, Riguan Yu, Ziniu Comparison of the Biochemical Composition and Nutritional Quality Between Diploid and Triploid Hong Kong Oysters, Crassostrea hongkongensis |
topic_facet |
Physiology Crassostrea hongkongensis diploid triploid biochemical compositions nutritional quality metabolic pathways FATTY-ACID-COMPOSITION PACIFIC OYSTER GLYCOGEN-CONTENT REPRODUCTIVE-CYCLE SEASONAL-VARIATION TAPES-PHILIPPINARUM MOLECULAR-CLONING CYTOCHALASIN-B GIGAS THUNBERG FUJIAN OYSTER |
description |
This study is the first systematic comparison of the biochemical composition and nutritional quality between diploid and triploid Hong Kong oysters, Crassostrea hongkongensis. Results showed that in the reproductive season, the glycogen content in five tissues (gill, mantle, adductor muscle, labial palps and gonad) was significantly higher (P < 0.05) in triploids than in diploids, with odds ratios (ORs) of 96.26, 60.17, 72.59, 53.56, and 128.52%, respectively. In the non-reproductive phase, significant differences in glycogen content (P < 0.05) between diploid and triploid oysters existed only in gill and gonad. In both diploid and triploid Hong Kong oysters, quantitative real-time PCR analysis of the glycogen synthesis gene (ChGS) and glycogen phosphorylase gene (ChGP) showed that the gene expression patterns matched the pattern of variation in glycogen content. Moreover, in both the reproductive and the non-reproductive phases, triploid Hong Kong oysters had a well balance of essential amino acids and were thus a well source of high-quality protein. Surprisingly, in both phases, significantly higher (P < 0.05) percentages of four essential fatty acids (a-linolenic acid, linoleic acid, eicosapentaenoic acid, and docosahexaenoic acid) were observed in triploids than in diploids. Additionally, the ratio of n-3/n-6 polyunsaturated fatty acids (PUFAs) was much higher in triploids than that in diploids. Variations in Biochemical composition were consistent with the relative expression of the citrate synthase gene (ChCS) and the a-ketoglutarate dehydrogenase gene (ChKD), which are key enzyme genes of the tricarboxylic acid cycle. Overall, the triploid Hong Kong oyster has a better nutritional value and taste than the diploid in terms of glycogen content, protein quality and fatty acid content. |
format |
Report |
author |
Qin, Yanping Zhang, Yuehuan Ma, Haitao Wu, Xiangwei Xiao, Shu Li, Jun Mo, Riguan Yu, Ziniu |
author_facet |
Qin, Yanping Zhang, Yuehuan Ma, Haitao Wu, Xiangwei Xiao, Shu Li, Jun Mo, Riguan Yu, Ziniu |
author_sort |
Qin, Yanping |
title |
Comparison of the Biochemical Composition and Nutritional Quality Between Diploid and Triploid Hong Kong Oysters, Crassostrea hongkongensis |
title_short |
Comparison of the Biochemical Composition and Nutritional Quality Between Diploid and Triploid Hong Kong Oysters, Crassostrea hongkongensis |
title_full |
Comparison of the Biochemical Composition and Nutritional Quality Between Diploid and Triploid Hong Kong Oysters, Crassostrea hongkongensis |
title_fullStr |
Comparison of the Biochemical Composition and Nutritional Quality Between Diploid and Triploid Hong Kong Oysters, Crassostrea hongkongensis |
title_full_unstemmed |
Comparison of the Biochemical Composition and Nutritional Quality Between Diploid and Triploid Hong Kong Oysters, Crassostrea hongkongensis |
title_sort |
comparison of the biochemical composition and nutritional quality between diploid and triploid hong kong oysters, crassostrea hongkongensis |
publisher |
FRONTIERS MEDIA SA |
publishDate |
2018 |
url |
http://ir.gig.ac.cn/handle/344008/40439 https://doi.org/10.3389/fphys.2018.01674 |
geographic |
Pacific |
geographic_facet |
Pacific |
genre |
Pacific oyster |
genre_facet |
Pacific oyster |
op_relation |
FRONTIERS IN PHYSIOLOGY http://ir.gig.ac.cn/handle/344008/40439 doi:10.3389/fphys.2018.01674 |
op_doi |
https://doi.org/10.3389/fphys.2018.01674 |
container_title |
Frontiers in Physiology |
container_volume |
9 |
_version_ |
1766162101343092736 |