Differential regulation of luteinizing hormone and follicle-stimulating hormone expression during ovarian development and under sexual steroid feedback in the European eel
Pituitary gonadotropins, luteinizing hormone (LH) and follicle-stimulating hormone (FSH) are, in teleosts as in mammals, under the control of hypothalamic factors and steroid feedbacks. In teleosts, feedback regulations largely vary depending on species and physiological stage. In the present study...
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ftcemoa:oai:irsteadoc.irstea.fr:PUB00017482 2023-05-15T13:28:12+02:00 Differential regulation of luteinizing hormone and follicle-stimulating hormone expression during ovarian development and under sexual steroid feedback in the European eel Schmitz, M. Aroua, S. Vidal, B. Le Belle, N. Elie, P. Dufour, S. SWEDISH UNIVERSITY OF AGRICULTURAL SCIENCE UMEA SWE MUSEUM NATIONAL D'HISTOIRE NATURELLE PARIS CEMAGREF BORDEAUX EPBX 2005 application/pdf https://irsteadoc.irstea.fr/cemoa/PUB00017482 Anglais eng https://irsteadoc.irstea.fr/cemoa/PUB00017482 Date de dépôt: 2005-09-12 - Tous les documents et informations contenus dans la base CemOA Publications sont protégés en vertu du droit de propriété intellectuelle, en particulier par le droit d'auteur. La personne consultant la base CemOA Publications peut visualiser, reproduire, ou stocker des copies des publications, à condition que l'information soit seulement pour son usage personnel et non commercial. L'utilisation des travaux universitaires est soumise à autorisation préalable de leurs auteurs. Toute information relative au signalement d'une publication contenue dans CemOA Publications doit inclure la citation bibliographique usuelle : Nom du ou des auteurs, titre et source du document, date et URL de la notice (dc_identifier). 15020 ANGUILLIDAE ANGUILLA ANGUILLA HORMONE GONADE COMPORTEMENT SEXUEL ENDOCRINOLOGIE HORMONES GONADS SEXUAL BEHAVIOUR ENDOCRINOLOGY Article de revue scientifique à comité de lecture 2005 ftcemoa 2021-06-29T08:39:28Z Pituitary gonadotropins, luteinizing hormone (LH) and follicle-stimulating hormone (FSH) are, in teleosts as in mammals, under the control of hypothalamic factors and steroid feedbacks. In teleosts, feedback regulations largely vary depending on species and physiological stage. In the present study the regulation of FSH and LH expression was investigated in the European eel, a fish of biological and phylogenetical interest as a representative of an early group of teleosts. The eel FSH beta subunit was cloned, sequenced and together with earlier isolated eel LH beta and glycoprotein hormone alpha (GP alpha) subunits used to study the differential regulation of LH and FSH. In situ hybridization indicated that FSH beta and LH beta are expressed by separate cells of the proximal pars distalis of the adenohypophysis, differently from the situation in mammals. The profiles of LH beta and FSH beta subunit expression were compared during experimental ovarian maturation, using dot-blot assays. Expression levels for LH beta and GP alpha increased throughout ovarian development with a positive correlation between these two subunits. Conversely, FSH beta mRNA levels decreased. To understand the role of sex steroids in these opposite variations, immature eels were treated with estradiol (E-2) and testosterone (T), both steroids being produced in eel ovaries during gonadal development. E-2 treatment induced increases in both LH beta and GP alpha mRNA levels, without any significant effect on FSH beta. In contrast, T treatment induced a decrease in FSH beta mRNA levels, without any significant effect on the other subunits. These data demonstrate that steroids exert a differential feedback on eel gonadotropin expression, with an E-2-specific positive feedback on LH and a T-specific negative feedback on FSH, leading to an opposite regulation of LH and FSH during ovarian development. Article in Journal/Newspaper Anguilla anguilla European eel Irstea Publications et Bases documentaires (Irstea@doc/CemOA) |
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Open Polar |
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Irstea Publications et Bases documentaires (Irstea@doc/CemOA) |
op_collection_id |
ftcemoa |
language |
English |
topic |
ANGUILLIDAE ANGUILLA ANGUILLA HORMONE GONADE COMPORTEMENT SEXUEL ENDOCRINOLOGIE HORMONES GONADS SEXUAL BEHAVIOUR ENDOCRINOLOGY |
spellingShingle |
ANGUILLIDAE ANGUILLA ANGUILLA HORMONE GONADE COMPORTEMENT SEXUEL ENDOCRINOLOGIE HORMONES GONADS SEXUAL BEHAVIOUR ENDOCRINOLOGY Schmitz, M. Aroua, S. Vidal, B. Le Belle, N. Elie, P. Dufour, S. Differential regulation of luteinizing hormone and follicle-stimulating hormone expression during ovarian development and under sexual steroid feedback in the European eel |
topic_facet |
ANGUILLIDAE ANGUILLA ANGUILLA HORMONE GONADE COMPORTEMENT SEXUEL ENDOCRINOLOGIE HORMONES GONADS SEXUAL BEHAVIOUR ENDOCRINOLOGY |
description |
Pituitary gonadotropins, luteinizing hormone (LH) and follicle-stimulating hormone (FSH) are, in teleosts as in mammals, under the control of hypothalamic factors and steroid feedbacks. In teleosts, feedback regulations largely vary depending on species and physiological stage. In the present study the regulation of FSH and LH expression was investigated in the European eel, a fish of biological and phylogenetical interest as a representative of an early group of teleosts. The eel FSH beta subunit was cloned, sequenced and together with earlier isolated eel LH beta and glycoprotein hormone alpha (GP alpha) subunits used to study the differential regulation of LH and FSH. In situ hybridization indicated that FSH beta and LH beta are expressed by separate cells of the proximal pars distalis of the adenohypophysis, differently from the situation in mammals. The profiles of LH beta and FSH beta subunit expression were compared during experimental ovarian maturation, using dot-blot assays. Expression levels for LH beta and GP alpha increased throughout ovarian development with a positive correlation between these two subunits. Conversely, FSH beta mRNA levels decreased. To understand the role of sex steroids in these opposite variations, immature eels were treated with estradiol (E-2) and testosterone (T), both steroids being produced in eel ovaries during gonadal development. E-2 treatment induced increases in both LH beta and GP alpha mRNA levels, without any significant effect on FSH beta. In contrast, T treatment induced a decrease in FSH beta mRNA levels, without any significant effect on the other subunits. These data demonstrate that steroids exert a differential feedback on eel gonadotropin expression, with an E-2-specific positive feedback on LH and a T-specific negative feedback on FSH, leading to an opposite regulation of LH and FSH during ovarian development. |
author2 |
SWEDISH UNIVERSITY OF AGRICULTURAL SCIENCE UMEA SWE MUSEUM NATIONAL D'HISTOIRE NATURELLE PARIS CEMAGREF BORDEAUX EPBX |
format |
Article in Journal/Newspaper |
author |
Schmitz, M. Aroua, S. Vidal, B. Le Belle, N. Elie, P. Dufour, S. |
author_facet |
Schmitz, M. Aroua, S. Vidal, B. Le Belle, N. Elie, P. Dufour, S. |
author_sort |
Schmitz, M. |
title |
Differential regulation of luteinizing hormone and follicle-stimulating hormone expression during ovarian development and under sexual steroid feedback in the European eel |
title_short |
Differential regulation of luteinizing hormone and follicle-stimulating hormone expression during ovarian development and under sexual steroid feedback in the European eel |
title_full |
Differential regulation of luteinizing hormone and follicle-stimulating hormone expression during ovarian development and under sexual steroid feedback in the European eel |
title_fullStr |
Differential regulation of luteinizing hormone and follicle-stimulating hormone expression during ovarian development and under sexual steroid feedback in the European eel |
title_full_unstemmed |
Differential regulation of luteinizing hormone and follicle-stimulating hormone expression during ovarian development and under sexual steroid feedback in the European eel |
title_sort |
differential regulation of luteinizing hormone and follicle-stimulating hormone expression during ovarian development and under sexual steroid feedback in the european eel |
publishDate |
2005 |
url |
https://irsteadoc.irstea.fr/cemoa/PUB00017482 |
genre |
Anguilla anguilla European eel |
genre_facet |
Anguilla anguilla European eel |
op_source |
15020 |
op_relation |
https://irsteadoc.irstea.fr/cemoa/PUB00017482 |
op_rights |
Date de dépôt: 2005-09-12 - Tous les documents et informations contenus dans la base CemOA Publications sont protégés en vertu du droit de propriété intellectuelle, en particulier par le droit d'auteur. La personne consultant la base CemOA Publications peut visualiser, reproduire, ou stocker des copies des publications, à condition que l'information soit seulement pour son usage personnel et non commercial. L'utilisation des travaux universitaires est soumise à autorisation préalable de leurs auteurs. Toute information relative au signalement d'une publication contenue dans CemOA Publications doit inclure la citation bibliographique usuelle : Nom du ou des auteurs, titre et source du document, date et URL de la notice (dc_identifier). |
_version_ |
1766402770488786944 |