Characterization of mollivirus Kamchatka, the First modern representative of the proposed molliviridae family of giant viruses
International audience Microbes trapped in permanently frozen paleosoils (permafrost) are the focus of increasing research in the context of global warming. Our previous investigations led to the discovery and reactivation of two Acanthamoeba-infecting giant viruses, Mollivirus sibericum and Pithovi...
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ftceafr:oai:HAL:hal-04290133v1 2024-09-15T18:08:09+00:00 Characterization of mollivirus Kamchatka, the First modern representative of the proposed molliviridae family of giant viruses Christo-Foroux, Eugene Alempic, Jean-Marie Lartigue, Audrey Santini, Sebastien Labadie, Karine Legendre, Matthieu Abergel, Chantal Claverie, Jean-Michel Information génomique et structurale (IGS) Aix Marseille Université (AMU)-Centre National de la Recherche Scientifique (CNRS) Institut de Microbiologie de la Méditerranée (IMM) Genoscope - Centre national de séquençage Evry (GENOSCOPE) Université Paris-Saclay-Direction de Recherche Fondamentale (CEA) (DRF (CEA)) Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA) French National Research Center (grant PRC1484-2018 to C. Abergel) 2020 https://hal.science/hal-04290133 https://doi.org/10.1128/jvi.01997-19 en eng HAL CCSD American Society for Microbiology info:eu-repo/semantics/altIdentifier/doi/10.1128/jvi.01997-19 hal-04290133 https://hal.science/hal-04290133 doi:10.1128/jvi.01997-19 ISSN: 0022-538X EISSN: 1098-5514 Journal of Virology https://hal.science/hal-04290133 Journal of Virology, 2020, 94 (8), pp.e01997-19. ⟨10.1128/jvi.01997-19⟩ https://journals.asm.org/doi/10.1128/jvi.01997-19 Acanthamoeba comparative genomics Kamchatka NCLDV paleovirology Arctic [SDV]Life Sciences [q-bio] info:eu-repo/semantics/article Journal articles 2020 ftceafr https://doi.org/10.1128/jvi.01997-19 2024-07-22T13:03:08Z International audience Microbes trapped in permanently frozen paleosoils (permafrost) are the focus of increasing research in the context of global warming. Our previous investigations led to the discovery and reactivation of two Acanthamoeba-infecting giant viruses, Mollivirus sibericum and Pithovirus sibericum, from a 30,000-year old permafrost layer. While several modern pithovirus strains have since been isolated, no contemporary mollivirus relative was found. We now describe Mollivirus kamchatka, a close relative to M. sibericum, isolated from surface soil sampled on the bank of the Kronotsky River in Kamchatka, Russian Federation. This discovery confirms that molliviruses have not gone extinct and are at least present in a distant subarctic continental location. This modern isolate exhibits a nucleocytoplasmic replication cycle identical to that of M. sibericum. Its spherical particle (0.6 μm in diameter) encloses a 648-kb GC-rich double-stranded DNA genome coding for 480 proteins, of which 61% are unique to these two molliviruses. The 461 homologous proteins are highly conserved (92% identical residues, on average), despite the presumed stasis of M. sibericum for the last 30,000 years. Selection pressure analyses show that most of these proteins contribute to virus fitness. The comparison of these first two molliviruses clarify their evolutionary relationship with the pandoraviruses, supporting their provisional classification in a distinct family, the Molliviridae, pending the eventual discovery of intermediary missing links better demonstrating their common ancestry. Article in Journal/Newspaper Global warming Kamchatka permafrost Subarctic HAL-CEA (Commissariat à l'énergie atomique et aux énergies alternatives) Journal of Virology 94 8 |
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Open Polar |
collection |
HAL-CEA (Commissariat à l'énergie atomique et aux énergies alternatives) |
op_collection_id |
ftceafr |
language |
English |
topic |
Acanthamoeba comparative genomics Kamchatka NCLDV paleovirology Arctic [SDV]Life Sciences [q-bio] |
spellingShingle |
Acanthamoeba comparative genomics Kamchatka NCLDV paleovirology Arctic [SDV]Life Sciences [q-bio] Christo-Foroux, Eugene Alempic, Jean-Marie Lartigue, Audrey Santini, Sebastien Labadie, Karine Legendre, Matthieu Abergel, Chantal Claverie, Jean-Michel Characterization of mollivirus Kamchatka, the First modern representative of the proposed molliviridae family of giant viruses |
topic_facet |
Acanthamoeba comparative genomics Kamchatka NCLDV paleovirology Arctic [SDV]Life Sciences [q-bio] |
description |
International audience Microbes trapped in permanently frozen paleosoils (permafrost) are the focus of increasing research in the context of global warming. Our previous investigations led to the discovery and reactivation of two Acanthamoeba-infecting giant viruses, Mollivirus sibericum and Pithovirus sibericum, from a 30,000-year old permafrost layer. While several modern pithovirus strains have since been isolated, no contemporary mollivirus relative was found. We now describe Mollivirus kamchatka, a close relative to M. sibericum, isolated from surface soil sampled on the bank of the Kronotsky River in Kamchatka, Russian Federation. This discovery confirms that molliviruses have not gone extinct and are at least present in a distant subarctic continental location. This modern isolate exhibits a nucleocytoplasmic replication cycle identical to that of M. sibericum. Its spherical particle (0.6 μm in diameter) encloses a 648-kb GC-rich double-stranded DNA genome coding for 480 proteins, of which 61% are unique to these two molliviruses. The 461 homologous proteins are highly conserved (92% identical residues, on average), despite the presumed stasis of M. sibericum for the last 30,000 years. Selection pressure analyses show that most of these proteins contribute to virus fitness. The comparison of these first two molliviruses clarify their evolutionary relationship with the pandoraviruses, supporting their provisional classification in a distinct family, the Molliviridae, pending the eventual discovery of intermediary missing links better demonstrating their common ancestry. |
author2 |
Information génomique et structurale (IGS) Aix Marseille Université (AMU)-Centre National de la Recherche Scientifique (CNRS) Institut de Microbiologie de la Méditerranée (IMM) Genoscope - Centre national de séquençage Evry (GENOSCOPE) Université Paris-Saclay-Direction de Recherche Fondamentale (CEA) (DRF (CEA)) Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA) French National Research Center (grant PRC1484-2018 to C. Abergel) |
format |
Article in Journal/Newspaper |
author |
Christo-Foroux, Eugene Alempic, Jean-Marie Lartigue, Audrey Santini, Sebastien Labadie, Karine Legendre, Matthieu Abergel, Chantal Claverie, Jean-Michel |
author_facet |
Christo-Foroux, Eugene Alempic, Jean-Marie Lartigue, Audrey Santini, Sebastien Labadie, Karine Legendre, Matthieu Abergel, Chantal Claverie, Jean-Michel |
author_sort |
Christo-Foroux, Eugene |
title |
Characterization of mollivirus Kamchatka, the First modern representative of the proposed molliviridae family of giant viruses |
title_short |
Characterization of mollivirus Kamchatka, the First modern representative of the proposed molliviridae family of giant viruses |
title_full |
Characterization of mollivirus Kamchatka, the First modern representative of the proposed molliviridae family of giant viruses |
title_fullStr |
Characterization of mollivirus Kamchatka, the First modern representative of the proposed molliviridae family of giant viruses |
title_full_unstemmed |
Characterization of mollivirus Kamchatka, the First modern representative of the proposed molliviridae family of giant viruses |
title_sort |
characterization of mollivirus kamchatka, the first modern representative of the proposed molliviridae family of giant viruses |
publisher |
HAL CCSD |
publishDate |
2020 |
url |
https://hal.science/hal-04290133 https://doi.org/10.1128/jvi.01997-19 |
genre |
Global warming Kamchatka permafrost Subarctic |
genre_facet |
Global warming Kamchatka permafrost Subarctic |
op_source |
ISSN: 0022-538X EISSN: 1098-5514 Journal of Virology https://hal.science/hal-04290133 Journal of Virology, 2020, 94 (8), pp.e01997-19. ⟨10.1128/jvi.01997-19⟩ https://journals.asm.org/doi/10.1128/jvi.01997-19 |
op_relation |
info:eu-repo/semantics/altIdentifier/doi/10.1128/jvi.01997-19 hal-04290133 https://hal.science/hal-04290133 doi:10.1128/jvi.01997-19 |
op_doi |
https://doi.org/10.1128/jvi.01997-19 |
container_title |
Journal of Virology |
container_volume |
94 |
container_issue |
8 |
_version_ |
1810445486969061376 |