Grazer-induced transcriptomic and metabolomic response of the chain-forming diatom Skeletonema marinoi

• Published in: The ISME Journal
• Main Authors: Amato, Alberto, Sabatino, Valeria, Nylund, Göran, Bergkvist, Johanna, Basu, Swaraj, Andersson, Mats, Sanges, Remo, Godhe, Anna, Kiorboe, Thomas, Selander, Erik, Ferrante, Maria
• Other Authors: Physiologie cellulaire et végétale (LPCV), Institut National de la Recherche Agronomique (INRA)-Centre National de la Recherche Scientifique (CNRS)-Université Grenoble Alpes 2016-2019 (UGA 2016-2019 )-Institut de Recherche Interdisciplinaire de Grenoble (IRIG), Direction de Recherche Fondamentale (CEA) (DRF (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Direction de Recherche Fondamentale (CEA) (DRF (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA), Integrative Marine Ecology, Stazione Zoologica Anton Dohrn (SZN), Department of Marine Sciences Gothenburg, Göteborgs Universitet = University of Gothenburg (GU), Department of Biological and Environmental Sciences Gothenburg, National Institute of Aquatic Resources, Danmarks Tekniske Universitet = Technical University of Denmark (DTU), Swedish Research Council VR 2015-05491; European Union under FP7-People 600407; Swedish Research Council Formas VR 217-2008-1719, European Project: 227799,EC:FP7:INFRA,FP7-INFRASTRUCTURES-2008-1,ASSEMBLE(2009)
• Format: Article in Journal/Newspaper
• Language: English
• Published: HAL CCSD 2018
• Subjects: [SDV]Life Sciences [q-bio]; [SDV.BBM]Life Sciences [q-bio]/Biochemistry; Molecular Biology; Calanus finmarchicus; Copepods
• Online Access: https://hal.science/hal-01852344; https://doi.org/10.1038/s41396-018-0094-0

International audience Diatoms and copepods are main actors in marine food webs. The prey–predator interactions between them affect bloom dynamics, shape marine ecosystems and impact the energy transfer to higher trophic levels. Recently it has been demonstrated that the presence of grazers may affect the diatom prey beyond the direct effect of grazing. Here, we investigated the response of the chain-forming centric diatom Skeletonema marinoi to grazer cues, including changes in morphology, gene expression and metabolic profile. S. marinoi cells were incubated with Calanus finmarchicus or with Centropages typicus and in both cases responded by reducing the chain length, whereas changes in gene expression indicated an activation of stress response, changes in the lipid and nitrogen metabolism, in cell cycle regulation and in frustule formation. Transcripts linked to G protein-coupled receptors and to nitric oxide synthesis were differentially expressed suggesting involvement of these signalling transduction pathways in the response. Downregulation of a lipoxygenase in the transcriptomic data and of its products in the metabolomic data also indicate an involvement of oxylipins. Our data contribute to a better understanding of the gene function in diatoms, providing information on the nature of genes implicated in the interaction with grazers, a crucial process in marine ecosystems.