Surface ocean metabarcoding confirms limited diversity in planktonic foraminifera but reveals unknown hyper-abundant lineages
International audience Since the advent of DNA metabarcoding surveys, the planktonic realm is considered a treasure trove of diversity, inhabited by a small number of abundant taxa, and a hugely diverse and taxonomically uncharacterized consortium of rare species. Here we assess if the apparent unde...
Published in: | Scientific Reports |
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Main Authors: | , , , , , , |
Other Authors: | , , , , , , , , , , , , , |
Format: | Article in Journal/Newspaper |
Language: | English |
Published: |
HAL CCSD
2018
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Subjects: | |
Online Access: | https://hal.sorbonne-universite.fr/hal-01718031 https://hal.sorbonne-universite.fr/hal-01718031/document https://hal.sorbonne-universite.fr/hal-01718031/file/s41598-018-20833-z.pdf https://doi.org/10.1038/s41598-018-20833-z |
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ftceafr:oai:HAL:hal-01718031v1 |
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record_format |
openpolar |
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Open Polar |
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HAL-CEA (Commissariat à l'énergie atomique et aux énergies alternatives) |
op_collection_id |
ftceafr |
language |
English |
topic |
[SDV.BID.SPT]Life Sciences [q-bio]/Biodiversity/Systematics Phylogenetics and taxonomy [SDV.MP.PRO]Life Sciences [q-bio]/Microbiology and Parasitology/Protistology [SDU.STU.OC]Sciences of the Universe [physics]/Earth Sciences/Oceanography [SDE]Environmental Sciences |
spellingShingle |
[SDV.BID.SPT]Life Sciences [q-bio]/Biodiversity/Systematics Phylogenetics and taxonomy [SDV.MP.PRO]Life Sciences [q-bio]/Microbiology and Parasitology/Protistology [SDU.STU.OC]Sciences of the Universe [physics]/Earth Sciences/Oceanography [SDE]Environmental Sciences Morard, Raphael Garet-Delmas, Marie-José Mahé, Frédéric Romac, Sarah Poulain, Julie Kucera, Michal de Vargas, Colomban Surface ocean metabarcoding confirms limited diversity in planktonic foraminifera but reveals unknown hyper-abundant lineages |
topic_facet |
[SDV.BID.SPT]Life Sciences [q-bio]/Biodiversity/Systematics Phylogenetics and taxonomy [SDV.MP.PRO]Life Sciences [q-bio]/Microbiology and Parasitology/Protistology [SDU.STU.OC]Sciences of the Universe [physics]/Earth Sciences/Oceanography [SDE]Environmental Sciences |
description |
International audience Since the advent of DNA metabarcoding surveys, the planktonic realm is considered a treasure trove of diversity, inhabited by a small number of abundant taxa, and a hugely diverse and taxonomically uncharacterized consortium of rare species. Here we assess if the apparent underestimation of plankton diversity applies universally. We target planktonic foraminifera, a group of protists whose known morphological diversity is limited, taxonomically resolved and linked to ribosomal DNA barcodes. We generated a pyrosequencing dataset of ~100,000 partial 18S rRNA foraminiferal sequences from 32 size fractioned photic-zone plankton samples collected at 8 stations in the Indian and Atlantic Oceans during the Tara Oceans expedition (2009–2012). We identified 69 genetic types belonging to 41 morphotaxa in our metabarcoding dataset. The diversity saturated at local and regional scale as well as in the three size fractions and the two depths sampled indicating that the diversity of foraminifera is modest and finite. The large majority of the newly discovered lineages occur in the small size fraction, neglected by classical taxonomy. These unknown lineages dominate the bulk [>0.8 µm] size fraction, implying that a considerable part of the planktonic foraminifera community biomass has its origin in unknown lineages. |
author2 |
Center for Marine Environmental Sciences Bremen (MARUM) Universität Bremen Adaptation et diversité en milieu marin (ADMM) Centre National de la Recherche Scientifique (CNRS)-Station biologique de Roscoff = Roscoff Marine Station (SBR) Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS) DIVersité et COnnectivité dans le paysage marin côtier (DIVCO) Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS)-Station biologique de Roscoff = Roscoff Marine Station (SBR) Laboratoire des symbioses tropicales et méditerranéennes (UMR LSTM) Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-Université Montpellier 1 (UM1)-Institut de Recherche pour le Développement (IRD)-Institut National de la Recherche Agronomique (INRA)-Université Montpellier 2 - Sciences et Techniques (UM2)-Université de Montpellier (UM)-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro) Evolution des Protistes et Ecosystèmes Pélagiques (EPEP) Genoscope - Centre national de séquençage Evry (GENOSCOPE) Université Paris-Saclay-Direction de Recherche Fondamentale (CEA) (DRF (CEA)) Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA) ANR-11-BTBR-0008,OCEANOMICS,Biotechnologies et bioressources pour la valorisation des écosystèmes marins planctoniques(2011) |
format |
Article in Journal/Newspaper |
author |
Morard, Raphael Garet-Delmas, Marie-José Mahé, Frédéric Romac, Sarah Poulain, Julie Kucera, Michal de Vargas, Colomban |
author_facet |
Morard, Raphael Garet-Delmas, Marie-José Mahé, Frédéric Romac, Sarah Poulain, Julie Kucera, Michal de Vargas, Colomban |
author_sort |
Morard, Raphael |
title |
Surface ocean metabarcoding confirms limited diversity in planktonic foraminifera but reveals unknown hyper-abundant lineages |
title_short |
Surface ocean metabarcoding confirms limited diversity in planktonic foraminifera but reveals unknown hyper-abundant lineages |
title_full |
Surface ocean metabarcoding confirms limited diversity in planktonic foraminifera but reveals unknown hyper-abundant lineages |
title_fullStr |
Surface ocean metabarcoding confirms limited diversity in planktonic foraminifera but reveals unknown hyper-abundant lineages |
title_full_unstemmed |
Surface ocean metabarcoding confirms limited diversity in planktonic foraminifera but reveals unknown hyper-abundant lineages |
title_sort |
surface ocean metabarcoding confirms limited diversity in planktonic foraminifera but reveals unknown hyper-abundant lineages |
publisher |
HAL CCSD |
publishDate |
2018 |
url |
https://hal.sorbonne-universite.fr/hal-01718031 https://hal.sorbonne-universite.fr/hal-01718031/document https://hal.sorbonne-universite.fr/hal-01718031/file/s41598-018-20833-z.pdf https://doi.org/10.1038/s41598-018-20833-z |
genre |
Planktonic foraminifera |
genre_facet |
Planktonic foraminifera |
op_source |
ISSN: 2045-2322 EISSN: 2045-2322 Scientific Reports https://hal.sorbonne-universite.fr/hal-01718031 Scientific Reports, 2018, 8, pp.2539. ⟨10.1038/s41598-018-20833-z⟩ |
op_relation |
info:eu-repo/semantics/altIdentifier/doi/10.1038/s41598-018-20833-z hal-01718031 https://hal.sorbonne-universite.fr/hal-01718031 https://hal.sorbonne-universite.fr/hal-01718031/document https://hal.sorbonne-universite.fr/hal-01718031/file/s41598-018-20833-z.pdf doi:10.1038/s41598-018-20833-z |
op_rights |
http://creativecommons.org/licenses/by/ info:eu-repo/semantics/OpenAccess |
op_doi |
https://doi.org/10.1038/s41598-018-20833-z |
container_title |
Scientific Reports |
container_volume |
8 |
container_issue |
1 |
_version_ |
1810472395690999808 |
spelling |
ftceafr:oai:HAL:hal-01718031v1 2024-09-15T18:30:52+00:00 Surface ocean metabarcoding confirms limited diversity in planktonic foraminifera but reveals unknown hyper-abundant lineages Morard, Raphael Garet-Delmas, Marie-José Mahé, Frédéric Romac, Sarah Poulain, Julie Kucera, Michal de Vargas, Colomban Center for Marine Environmental Sciences Bremen (MARUM) Universität Bremen Adaptation et diversité en milieu marin (ADMM) Centre National de la Recherche Scientifique (CNRS)-Station biologique de Roscoff = Roscoff Marine Station (SBR) Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS) DIVersité et COnnectivité dans le paysage marin côtier (DIVCO) Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS)-Station biologique de Roscoff = Roscoff Marine Station (SBR) Laboratoire des symbioses tropicales et méditerranéennes (UMR LSTM) Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-Université Montpellier 1 (UM1)-Institut de Recherche pour le Développement (IRD)-Institut National de la Recherche Agronomique (INRA)-Université Montpellier 2 - Sciences et Techniques (UM2)-Université de Montpellier (UM)-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro) Evolution des Protistes et Ecosystèmes Pélagiques (EPEP) Genoscope - Centre national de séquençage Evry (GENOSCOPE) Université Paris-Saclay-Direction de Recherche Fondamentale (CEA) (DRF (CEA)) Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA) ANR-11-BTBR-0008,OCEANOMICS,Biotechnologies et bioressources pour la valorisation des écosystèmes marins planctoniques(2011) 2018 https://hal.sorbonne-universite.fr/hal-01718031 https://hal.sorbonne-universite.fr/hal-01718031/document https://hal.sorbonne-universite.fr/hal-01718031/file/s41598-018-20833-z.pdf https://doi.org/10.1038/s41598-018-20833-z en eng HAL CCSD Nature Publishing Group info:eu-repo/semantics/altIdentifier/doi/10.1038/s41598-018-20833-z hal-01718031 https://hal.sorbonne-universite.fr/hal-01718031 https://hal.sorbonne-universite.fr/hal-01718031/document https://hal.sorbonne-universite.fr/hal-01718031/file/s41598-018-20833-z.pdf doi:10.1038/s41598-018-20833-z http://creativecommons.org/licenses/by/ info:eu-repo/semantics/OpenAccess ISSN: 2045-2322 EISSN: 2045-2322 Scientific Reports https://hal.sorbonne-universite.fr/hal-01718031 Scientific Reports, 2018, 8, pp.2539. ⟨10.1038/s41598-018-20833-z⟩ [SDV.BID.SPT]Life Sciences [q-bio]/Biodiversity/Systematics Phylogenetics and taxonomy [SDV.MP.PRO]Life Sciences [q-bio]/Microbiology and Parasitology/Protistology [SDU.STU.OC]Sciences of the Universe [physics]/Earth Sciences/Oceanography [SDE]Environmental Sciences info:eu-repo/semantics/article Journal articles 2018 ftceafr https://doi.org/10.1038/s41598-018-20833-z 2024-07-22T13:27:26Z International audience Since the advent of DNA metabarcoding surveys, the planktonic realm is considered a treasure trove of diversity, inhabited by a small number of abundant taxa, and a hugely diverse and taxonomically uncharacterized consortium of rare species. Here we assess if the apparent underestimation of plankton diversity applies universally. We target planktonic foraminifera, a group of protists whose known morphological diversity is limited, taxonomically resolved and linked to ribosomal DNA barcodes. We generated a pyrosequencing dataset of ~100,000 partial 18S rRNA foraminiferal sequences from 32 size fractioned photic-zone plankton samples collected at 8 stations in the Indian and Atlantic Oceans during the Tara Oceans expedition (2009–2012). We identified 69 genetic types belonging to 41 morphotaxa in our metabarcoding dataset. The diversity saturated at local and regional scale as well as in the three size fractions and the two depths sampled indicating that the diversity of foraminifera is modest and finite. The large majority of the newly discovered lineages occur in the small size fraction, neglected by classical taxonomy. These unknown lineages dominate the bulk [>0.8 µm] size fraction, implying that a considerable part of the planktonic foraminifera community biomass has its origin in unknown lineages. Article in Journal/Newspaper Planktonic foraminifera HAL-CEA (Commissariat à l'énergie atomique et aux énergies alternatives) Scientific Reports 8 1 |