Protofibril assemblies of the arctic, dutch, and flemish mutants of the alzheimer's Aβ 1-40 peptide
Using a coarse-grained model of the Aβ peptide, we analyze the Arctic (E22G), Dutch (E22Q), and Flemish (A21G) familial Alzheimer's disease (FAD) mutants for any changes in the stability of amyloid assemblies with respect to the wild-type (WT) sequence. Based on a structural reference state of...
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Language: | English |
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2008
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ftcdlib:qt8qw2m08v 2023-05-15T14:25:42+02:00 Protofibril assemblies of the arctic, dutch, and flemish mutants of the alzheimer's Aβ 1-40 peptide Fawzi, NL Kohlstedt, KL Okabe, Y Head-Gordon, T 2007 - 2016 2008-03-15 application/pdf http://www.escholarship.org/uc/item/8qw2m08v english eng eScholarship, University of California qt8qw2m08v http://www.escholarship.org/uc/item/8qw2m08v public Fawzi, NL; Kohlstedt, KL; Okabe, Y; & Head-Gordon, T. (2008). Protofibril assemblies of the arctic, dutch, and flemish mutants of the alzheimer's Aβ 1-40 peptide. Biophysical Journal, 94(6), 2007 - 2016. doi:10.1529/biophysj.107.121467. UC Berkeley: Retrieved from: http://www.escholarship.org/uc/item/8qw2m08v article 2008 ftcdlib https://doi.org/10.1529/biophysj.107.121467 2018-11-09T23:51:57Z Using a coarse-grained model of the Aβ peptide, we analyze the Arctic (E22G), Dutch (E22Q), and Flemish (A21G) familial Alzheimer's disease (FAD) mutants for any changes in the stability of amyloid assemblies with respect to the wild-type (WT) sequence. Based on a structural reference state of two protofilaments aligned to create the ''agitated'' protofibril as determined by solid-state NMR, we determine free energy trends for Aβ assemblies for the WT and FAD familial sequences. We find that the structural characteristics and oligomer size of the critical nucleus vary dramatically among the hereditary mutants. The Arctic mutant's disorder in the turn region introduces new stabilizing interactions that better align the two protofilaments, yielding a well-defined protofibril axis at relatively small oligomer sizes with respect to WT. By contrast, the critical nucleus for the Flemish mutant is beyond the 20 chains characterized in this study, thereby showing a strong shift in the equilibrium toward monomers with respect to larger protofibril assemblies. The Dutch mutant forms more ordered protofilaments than WT, but exhibits greater disorder in protofibril structure that includes an alternative polymorph of the WT fibril. An important conclusion of this work is that the Dutch mutant does not support the agitated protofibril assembly. We discuss the implications of the structural ensembles and free energy profiles for the FAD mutants in regards to interpretation of the kinetics of fibril assembly using chromatography and dye-binding experiments. © 2008 by the Biophysical Society. Article in Journal/Newspaper Arctic Arctic University of California: eScholarship Arctic Biophysical Journal 94 6 2007 2016 |
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Open Polar |
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University of California: eScholarship |
op_collection_id |
ftcdlib |
language |
English |
description |
Using a coarse-grained model of the Aβ peptide, we analyze the Arctic (E22G), Dutch (E22Q), and Flemish (A21G) familial Alzheimer's disease (FAD) mutants for any changes in the stability of amyloid assemblies with respect to the wild-type (WT) sequence. Based on a structural reference state of two protofilaments aligned to create the ''agitated'' protofibril as determined by solid-state NMR, we determine free energy trends for Aβ assemblies for the WT and FAD familial sequences. We find that the structural characteristics and oligomer size of the critical nucleus vary dramatically among the hereditary mutants. The Arctic mutant's disorder in the turn region introduces new stabilizing interactions that better align the two protofilaments, yielding a well-defined protofibril axis at relatively small oligomer sizes with respect to WT. By contrast, the critical nucleus for the Flemish mutant is beyond the 20 chains characterized in this study, thereby showing a strong shift in the equilibrium toward monomers with respect to larger protofibril assemblies. The Dutch mutant forms more ordered protofilaments than WT, but exhibits greater disorder in protofibril structure that includes an alternative polymorph of the WT fibril. An important conclusion of this work is that the Dutch mutant does not support the agitated protofibril assembly. We discuss the implications of the structural ensembles and free energy profiles for the FAD mutants in regards to interpretation of the kinetics of fibril assembly using chromatography and dye-binding experiments. © 2008 by the Biophysical Society. |
format |
Article in Journal/Newspaper |
author |
Fawzi, NL Kohlstedt, KL Okabe, Y Head-Gordon, T |
spellingShingle |
Fawzi, NL Kohlstedt, KL Okabe, Y Head-Gordon, T Protofibril assemblies of the arctic, dutch, and flemish mutants of the alzheimer's Aβ 1-40 peptide |
author_facet |
Fawzi, NL Kohlstedt, KL Okabe, Y Head-Gordon, T |
author_sort |
Fawzi, NL |
title |
Protofibril assemblies of the arctic, dutch, and flemish mutants of the alzheimer's Aβ 1-40 peptide |
title_short |
Protofibril assemblies of the arctic, dutch, and flemish mutants of the alzheimer's Aβ 1-40 peptide |
title_full |
Protofibril assemblies of the arctic, dutch, and flemish mutants of the alzheimer's Aβ 1-40 peptide |
title_fullStr |
Protofibril assemblies of the arctic, dutch, and flemish mutants of the alzheimer's Aβ 1-40 peptide |
title_full_unstemmed |
Protofibril assemblies of the arctic, dutch, and flemish mutants of the alzheimer's Aβ 1-40 peptide |
title_sort |
protofibril assemblies of the arctic, dutch, and flemish mutants of the alzheimer's aβ 1-40 peptide |
publisher |
eScholarship, University of California |
publishDate |
2008 |
url |
http://www.escholarship.org/uc/item/8qw2m08v |
op_coverage |
2007 - 2016 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic Arctic |
genre_facet |
Arctic Arctic |
op_source |
Fawzi, NL; Kohlstedt, KL; Okabe, Y; & Head-Gordon, T. (2008). Protofibril assemblies of the arctic, dutch, and flemish mutants of the alzheimer's Aβ 1-40 peptide. Biophysical Journal, 94(6), 2007 - 2016. doi:10.1529/biophysj.107.121467. UC Berkeley: Retrieved from: http://www.escholarship.org/uc/item/8qw2m08v |
op_relation |
qt8qw2m08v http://www.escholarship.org/uc/item/8qw2m08v |
op_rights |
public |
op_doi |
https://doi.org/10.1529/biophysj.107.121467 |
container_title |
Biophysical Journal |
container_volume |
94 |
container_issue |
6 |
container_start_page |
2007 |
op_container_end_page |
2016 |
_version_ |
1766298159126937600 |