Metagenomic insights into diazotrophic communities across Arctic glacier forefields
© FEMS 2018. Microbial nitrogen fixation is crucial for building labile nitrogen stocks and facilitating higher plant colonisation in oligotrophic glacier forefield soils. Here, the diazotrophic bacterial community structure across four Arctic glacier forefields was investigated using metagenomic an...
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ftcdlib:qt7kc4x6f3 2023-05-15T14:25:43+02:00 Metagenomic insights into diazotrophic communities across Arctic glacier forefields Nash, MV Anesio, AM Barker, G Tranter, M Varliero, G Eloe-Fadrosh, EA Nielsen, T Turpin-Jelfs, T Benning, LG Sánchez-Baracaldo, P 2018-09-01 application/pdf http://www.escholarship.org/uc/item/7kc4x6f3 english eng eScholarship, University of California qt7kc4x6f3 http://www.escholarship.org/uc/item/7kc4x6f3 public Nash, MV; Anesio, AM; Barker, G; Tranter, M; Varliero, G; Eloe-Fadrosh, EA; et al.(2018). Metagenomic insights into diazotrophic communities across Arctic glacier forefields. FEMS Microbiology Ecology, 94(9). doi:10.1093/femsec/fiy114. Lawrence Berkeley National Laboratory: Retrieved from: http://www.escholarship.org/uc/item/7kc4x6f3 article 2018 ftcdlib https://doi.org/10.1093/femsec/fiy114 2018-08-10T22:52:03Z © FEMS 2018. Microbial nitrogen fixation is crucial for building labile nitrogen stocks and facilitating higher plant colonisation in oligotrophic glacier forefield soils. Here, the diazotrophic bacterial community structure across four Arctic glacier forefields was investigated using metagenomic analysis. In total, 70 soil metagenomes were used for taxonomic interpretation based on 185 nitrogenase (nif) sequences, extracted from assembled contigs. The low number of recovered genes highlights the need for deeper sequencing in some diverse samples, to uncover the complete microbial populations. A key group of forefield diazotrophs, found throughout the forefields, was identified using a nifH phylogeny, associated with nifH Cluster I and III. Sequences related most closely to groups including Alphaproteobacteria, Betaproteobacteria, Cyanobacteria and Firmicutes. Using multiple nif genes in a Last Common Ancestor analysis revealed a diverse range of diazotrophs across the forefields. Key organisms identified across the forefields included Nostoc, Geobacter, Polaromonas and Frankia. Nitrogen fixers that are symbiotic with plants were also identified, through the presence of root associated diazotrophs, which fix nitrogen in return for reduced carbon. Additional nitrogen fixers identified in forefield soils were metabolically diverse, including fermentative and sulphur cycling bacteria, halophiles and anaerobes. Article in Journal/Newspaper Arctic Arctic University of California: eScholarship Arctic FEMS Microbiology Ecology 94 9 |
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description |
© FEMS 2018. Microbial nitrogen fixation is crucial for building labile nitrogen stocks and facilitating higher plant colonisation in oligotrophic glacier forefield soils. Here, the diazotrophic bacterial community structure across four Arctic glacier forefields was investigated using metagenomic analysis. In total, 70 soil metagenomes were used for taxonomic interpretation based on 185 nitrogenase (nif) sequences, extracted from assembled contigs. The low number of recovered genes highlights the need for deeper sequencing in some diverse samples, to uncover the complete microbial populations. A key group of forefield diazotrophs, found throughout the forefields, was identified using a nifH phylogeny, associated with nifH Cluster I and III. Sequences related most closely to groups including Alphaproteobacteria, Betaproteobacteria, Cyanobacteria and Firmicutes. Using multiple nif genes in a Last Common Ancestor analysis revealed a diverse range of diazotrophs across the forefields. Key organisms identified across the forefields included Nostoc, Geobacter, Polaromonas and Frankia. Nitrogen fixers that are symbiotic with plants were also identified, through the presence of root associated diazotrophs, which fix nitrogen in return for reduced carbon. Additional nitrogen fixers identified in forefield soils were metabolically diverse, including fermentative and sulphur cycling bacteria, halophiles and anaerobes. |
format |
Article in Journal/Newspaper |
author |
Nash, MV Anesio, AM Barker, G Tranter, M Varliero, G Eloe-Fadrosh, EA Nielsen, T Turpin-Jelfs, T Benning, LG Sánchez-Baracaldo, P |
spellingShingle |
Nash, MV Anesio, AM Barker, G Tranter, M Varliero, G Eloe-Fadrosh, EA Nielsen, T Turpin-Jelfs, T Benning, LG Sánchez-Baracaldo, P Metagenomic insights into diazotrophic communities across Arctic glacier forefields |
author_facet |
Nash, MV Anesio, AM Barker, G Tranter, M Varliero, G Eloe-Fadrosh, EA Nielsen, T Turpin-Jelfs, T Benning, LG Sánchez-Baracaldo, P |
author_sort |
Nash, MV |
title |
Metagenomic insights into diazotrophic communities across Arctic glacier forefields |
title_short |
Metagenomic insights into diazotrophic communities across Arctic glacier forefields |
title_full |
Metagenomic insights into diazotrophic communities across Arctic glacier forefields |
title_fullStr |
Metagenomic insights into diazotrophic communities across Arctic glacier forefields |
title_full_unstemmed |
Metagenomic insights into diazotrophic communities across Arctic glacier forefields |
title_sort |
metagenomic insights into diazotrophic communities across arctic glacier forefields |
publisher |
eScholarship, University of California |
publishDate |
2018 |
url |
http://www.escholarship.org/uc/item/7kc4x6f3 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic Arctic |
genre_facet |
Arctic Arctic |
op_source |
Nash, MV; Anesio, AM; Barker, G; Tranter, M; Varliero, G; Eloe-Fadrosh, EA; et al.(2018). Metagenomic insights into diazotrophic communities across Arctic glacier forefields. FEMS Microbiology Ecology, 94(9). doi:10.1093/femsec/fiy114. Lawrence Berkeley National Laboratory: Retrieved from: http://www.escholarship.org/uc/item/7kc4x6f3 |
op_relation |
qt7kc4x6f3 http://www.escholarship.org/uc/item/7kc4x6f3 |
op_rights |
public |
op_doi |
https://doi.org/10.1093/femsec/fiy114 |
container_title |
FEMS Microbiology Ecology |
container_volume |
94 |
container_issue |
9 |
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1766298161371938816 |