The Effects of Ocean Acidification on the Development, Behavior and Survival of Marine Fish Eggs and Larvae Inferred from Laboratory and Natural Experiments
The physiology, development, behavior and survival of the early life history stagesof marine fish are challenged by increasing carbon dioxide concentrations in the ocean,known as ocean acidification. A widespread effect of elevated pCO2 on fish larvae isincreased otolith size. To understand the func...
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ftcdlib:qt5mp4g3x1 2023-05-15T17:50:11+02:00 The Effects of Ocean Acidification on the Development, Behavior and Survival of Marine Fish Eggs and Larvae Inferred from Laboratory and Natural Experiments Shen, Sara 111 2016-01-01 application/pdf http://www.escholarship.org/uc/item/5mp4g3x1 en eng eScholarship, University of California http://www.escholarship.org/uc/item/5mp4g3x1 qt5mp4g3x1 public Shen, Sara. (2016). The Effects of Ocean Acidification on the Development, Behavior and Survival of Marine Fish Eggs and Larvae Inferred from Laboratory and Natural Experiments. UC San Diego: Oceanography. Retrieved from: http://www.escholarship.org/uc/item/5mp4g3x1 Biological oceanography Ecology Early life history Fish Ocean acidification Otolith Physiology Upwelling dissertation 2016 ftcdlib 2016-12-23T23:51:00Z The physiology, development, behavior and survival of the early life history stagesof marine fish are challenged by increasing carbon dioxide concentrations in the ocean,known as ocean acidification. A widespread effect of elevated pCO2 on fish larvae isincreased otolith size. To understand the functional consequences of larger otoliths on thevestibular system of fish larvae, Chapter 2 investigated the vestibulo-ocular reflex (VOR)of white seabass (Atractoscion nobilis) larvae reared at 2500 μatm pCO2. The VOR isan otolith-dependent response in fish that stabilizes vision during body movement. Larvaereared at high pCO2 possessed saccular and utricular otoliths that were 17% and 38% larger in size. Despite the increased otolith size, the gain of the VOR, which describes theratio of eye to head amplitude, was not statistically different between treatment (0.39 +- 0.05, n = 28) and control (0.30 +- 0.03, n = 20) larvae.Fish spawning habitat and survival of offspring are greatly influenced by environmentalconditions. In Chapter 3, the effects of pCO2 on the spawning habitat of Anchoveta(Engraulis ringens) and mortality of eggs and early stage larvae were investigated. Eggs,larvae, and oceanographic data, were collected across an onshore-offshore gradient in pCO2 that ranged from 167-1392 μatm. pCO2 was statistically significant in explaining egg presence. The abundance of eggs and relative absence of larvae at high pCO2 suggests that Anchoveta preferentially spawned at high pCO2 (>800 μatm) and that these eggs had lower survival.Fish living in a high-pCO2 world may have to spend more energy on acid-basebalance. Chapter 4 explores the effects of elevated pCO2 on the oxygen consumption rate(OCR) and abundance of Na+-K+-ATPase (NKA) proteins in white seabass larvae reared at 2000 μatm pCO2. OCR, a proxy for aerobic metabolic rate, did not differ significantlybetween larvae reared at present-day (0.18 +- 0.03 μL O2 individual-1 h-1, n = 80) and future (0.19 +- 0.03 μL O2 individual-1 h-1, n = 80) pCO2. Consistent with this finding, the relative abundance of NKA proteins that fuel important ion exchangers for acid-base balance did not differ between control and treatment larvae in Western blot and immunohistochemistry analyses. Mass and length were also unchanged at high pCO2, suggesting larvae were physiologically robust in these variables to ocean acidification conditions. Doctoral or Postdoctoral Thesis Ocean acidification University of California: eScholarship |
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Open Polar |
collection |
University of California: eScholarship |
op_collection_id |
ftcdlib |
language |
English |
topic |
Biological oceanography Ecology Early life history Fish Ocean acidification Otolith Physiology Upwelling |
spellingShingle |
Biological oceanography Ecology Early life history Fish Ocean acidification Otolith Physiology Upwelling Shen, Sara The Effects of Ocean Acidification on the Development, Behavior and Survival of Marine Fish Eggs and Larvae Inferred from Laboratory and Natural Experiments |
topic_facet |
Biological oceanography Ecology Early life history Fish Ocean acidification Otolith Physiology Upwelling |
description |
The physiology, development, behavior and survival of the early life history stagesof marine fish are challenged by increasing carbon dioxide concentrations in the ocean,known as ocean acidification. A widespread effect of elevated pCO2 on fish larvae isincreased otolith size. To understand the functional consequences of larger otoliths on thevestibular system of fish larvae, Chapter 2 investigated the vestibulo-ocular reflex (VOR)of white seabass (Atractoscion nobilis) larvae reared at 2500 μatm pCO2. The VOR isan otolith-dependent response in fish that stabilizes vision during body movement. Larvaereared at high pCO2 possessed saccular and utricular otoliths that were 17% and 38% larger in size. Despite the increased otolith size, the gain of the VOR, which describes theratio of eye to head amplitude, was not statistically different between treatment (0.39 +- 0.05, n = 28) and control (0.30 +- 0.03, n = 20) larvae.Fish spawning habitat and survival of offspring are greatly influenced by environmentalconditions. In Chapter 3, the effects of pCO2 on the spawning habitat of Anchoveta(Engraulis ringens) and mortality of eggs and early stage larvae were investigated. Eggs,larvae, and oceanographic data, were collected across an onshore-offshore gradient in pCO2 that ranged from 167-1392 μatm. pCO2 was statistically significant in explaining egg presence. The abundance of eggs and relative absence of larvae at high pCO2 suggests that Anchoveta preferentially spawned at high pCO2 (>800 μatm) and that these eggs had lower survival.Fish living in a high-pCO2 world may have to spend more energy on acid-basebalance. Chapter 4 explores the effects of elevated pCO2 on the oxygen consumption rate(OCR) and abundance of Na+-K+-ATPase (NKA) proteins in white seabass larvae reared at 2000 μatm pCO2. OCR, a proxy for aerobic metabolic rate, did not differ significantlybetween larvae reared at present-day (0.18 +- 0.03 μL O2 individual-1 h-1, n = 80) and future (0.19 +- 0.03 μL O2 individual-1 h-1, n = 80) pCO2. Consistent with this finding, the relative abundance of NKA proteins that fuel important ion exchangers for acid-base balance did not differ between control and treatment larvae in Western blot and immunohistochemistry analyses. Mass and length were also unchanged at high pCO2, suggesting larvae were physiologically robust in these variables to ocean acidification conditions. |
format |
Doctoral or Postdoctoral Thesis |
author |
Shen, Sara |
author_facet |
Shen, Sara |
author_sort |
Shen, Sara |
title |
The Effects of Ocean Acidification on the Development, Behavior and Survival of Marine Fish Eggs and Larvae Inferred from Laboratory and Natural Experiments |
title_short |
The Effects of Ocean Acidification on the Development, Behavior and Survival of Marine Fish Eggs and Larvae Inferred from Laboratory and Natural Experiments |
title_full |
The Effects of Ocean Acidification on the Development, Behavior and Survival of Marine Fish Eggs and Larvae Inferred from Laboratory and Natural Experiments |
title_fullStr |
The Effects of Ocean Acidification on the Development, Behavior and Survival of Marine Fish Eggs and Larvae Inferred from Laboratory and Natural Experiments |
title_full_unstemmed |
The Effects of Ocean Acidification on the Development, Behavior and Survival of Marine Fish Eggs and Larvae Inferred from Laboratory and Natural Experiments |
title_sort |
effects of ocean acidification on the development, behavior and survival of marine fish eggs and larvae inferred from laboratory and natural experiments |
publisher |
eScholarship, University of California |
publishDate |
2016 |
url |
http://www.escholarship.org/uc/item/5mp4g3x1 |
op_coverage |
111 |
genre |
Ocean acidification |
genre_facet |
Ocean acidification |
op_source |
Shen, Sara. (2016). The Effects of Ocean Acidification on the Development, Behavior and Survival of Marine Fish Eggs and Larvae Inferred from Laboratory and Natural Experiments. UC San Diego: Oceanography. Retrieved from: http://www.escholarship.org/uc/item/5mp4g3x1 |
op_relation |
http://www.escholarship.org/uc/item/5mp4g3x1 qt5mp4g3x1 |
op_rights |
public |
_version_ |
1766156831364743168 |