Characterization of bacterial communities in lithobionts and soil niches from Victoria Valley, Antarctica
Here we provide the first exploration of microbial diversity from three distinct Victoria Valley edaphic habitats, namely lithobionts (hypoliths, endoliths) and surface soils. Using a combination of terminal restriction fragment length polymorphism (T-RFLP) analysis and 16S rRNA gene amplicon pyrose...
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Language: | English |
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ftcdlib:qt33v85617 2023-05-15T13:50:54+02:00 Characterization of bacterial communities in lithobionts and soil niches from Victoria Valley, Antarctica Van Goethem, MW Makhalanyane, TP Valverde, A Cary, SC Cowan, DA 1 - 22 2016-03-04 application/pdf http://www.escholarship.org/uc/item/33v85617 english eng eScholarship, University of California qt33v85617 http://www.escholarship.org/uc/item/33v85617 public Van Goethem, MW; Makhalanyane, TP; Valverde, A; Cary, SC; & Cowan, DA. (2016). Characterization of bacterial communities in lithobionts and soil niches from Victoria Valley, Antarctica. FEMS Microbiology Ecology, 92(4), 1 - 22. doi:10.1093/femsec/fiw051. Lawrence Berkeley National Laboratory: Retrieved from: http://www.escholarship.org/uc/item/33v85617 article 2016 ftcdlib https://doi.org/10.1093/femsec/fiw051 2018-07-13T22:56:20Z Here we provide the first exploration of microbial diversity from three distinct Victoria Valley edaphic habitats, namely lithobionts (hypoliths, endoliths) and surface soils. Using a combination of terminal restriction fragment length polymorphism (T-RFLP) analysis and 16S rRNA gene amplicon pyrosequencing we assess community structure and diversity patterns, respectively. Our analysis revealed that habitat type (endolithic vs hypolithic vs surface soils) significantly influenced bacterial community composition, even though dominant phyla such as Actinobacteria (41% of total reads) were common to all samples. Consistent with previous surveys in other Dry Valley ecosystems, we found that lithobionts were colonised by a few highly-dominant phylotypes (such as Gemmatimonas and Leptolyngbya). Our analyses also show that soil bacteria were more diverse and evenly distributed than initially expected based on previous evidence. In contrast to total bacteria, the distribution of Cyanobacteria was not strongly influenced by habitat type, although soil- and endolith-specific cyanobacterial lineages were found. The detection of cyanobacterial lineages in these habitats appears to be influenced by the dispersal of aquatic inocula from lacustrine communities or benthic mats which are abundant in Victoria Valley. Together, our results provide insights into the phylogenetic variation and community structure across niche habitats in Victoria Valley. Article in Journal/Newspaper Antarc* Antarctica University of California: eScholarship Victoria Valley ENVELOPE(162.000,162.000,-77.383,-77.383) FEMS Microbiology Ecology 92 4 fiw051 |
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Open Polar |
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University of California: eScholarship |
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ftcdlib |
language |
English |
description |
Here we provide the first exploration of microbial diversity from three distinct Victoria Valley edaphic habitats, namely lithobionts (hypoliths, endoliths) and surface soils. Using a combination of terminal restriction fragment length polymorphism (T-RFLP) analysis and 16S rRNA gene amplicon pyrosequencing we assess community structure and diversity patterns, respectively. Our analysis revealed that habitat type (endolithic vs hypolithic vs surface soils) significantly influenced bacterial community composition, even though dominant phyla such as Actinobacteria (41% of total reads) were common to all samples. Consistent with previous surveys in other Dry Valley ecosystems, we found that lithobionts were colonised by a few highly-dominant phylotypes (such as Gemmatimonas and Leptolyngbya). Our analyses also show that soil bacteria were more diverse and evenly distributed than initially expected based on previous evidence. In contrast to total bacteria, the distribution of Cyanobacteria was not strongly influenced by habitat type, although soil- and endolith-specific cyanobacterial lineages were found. The detection of cyanobacterial lineages in these habitats appears to be influenced by the dispersal of aquatic inocula from lacustrine communities or benthic mats which are abundant in Victoria Valley. Together, our results provide insights into the phylogenetic variation and community structure across niche habitats in Victoria Valley. |
format |
Article in Journal/Newspaper |
author |
Van Goethem, MW Makhalanyane, TP Valverde, A Cary, SC Cowan, DA |
spellingShingle |
Van Goethem, MW Makhalanyane, TP Valverde, A Cary, SC Cowan, DA Characterization of bacterial communities in lithobionts and soil niches from Victoria Valley, Antarctica |
author_facet |
Van Goethem, MW Makhalanyane, TP Valverde, A Cary, SC Cowan, DA |
author_sort |
Van Goethem, MW |
title |
Characterization of bacterial communities in lithobionts and soil niches from Victoria Valley, Antarctica |
title_short |
Characterization of bacterial communities in lithobionts and soil niches from Victoria Valley, Antarctica |
title_full |
Characterization of bacterial communities in lithobionts and soil niches from Victoria Valley, Antarctica |
title_fullStr |
Characterization of bacterial communities in lithobionts and soil niches from Victoria Valley, Antarctica |
title_full_unstemmed |
Characterization of bacterial communities in lithobionts and soil niches from Victoria Valley, Antarctica |
title_sort |
characterization of bacterial communities in lithobionts and soil niches from victoria valley, antarctica |
publisher |
eScholarship, University of California |
publishDate |
2016 |
url |
http://www.escholarship.org/uc/item/33v85617 |
op_coverage |
1 - 22 |
long_lat |
ENVELOPE(162.000,162.000,-77.383,-77.383) |
geographic |
Victoria Valley |
geographic_facet |
Victoria Valley |
genre |
Antarc* Antarctica |
genre_facet |
Antarc* Antarctica |
op_source |
Van Goethem, MW; Makhalanyane, TP; Valverde, A; Cary, SC; & Cowan, DA. (2016). Characterization of bacterial communities in lithobionts and soil niches from Victoria Valley, Antarctica. FEMS Microbiology Ecology, 92(4), 1 - 22. doi:10.1093/femsec/fiw051. Lawrence Berkeley National Laboratory: Retrieved from: http://www.escholarship.org/uc/item/33v85617 |
op_relation |
qt33v85617 http://www.escholarship.org/uc/item/33v85617 |
op_rights |
public |
op_doi |
https://doi.org/10.1093/femsec/fiw051 |
container_title |
FEMS Microbiology Ecology |
container_volume |
92 |
container_issue |
4 |
container_start_page |
fiw051 |
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1766254265418907648 |