Genomic methods and microbiological technologies for profiling novel and extreme environments for the extreme microbiome project (XMP)
© 2017, Association of Biomolecular Resource Facilities. All rights reserved. The Extreme Microbiome Project (XMP) is a project launched by the Association of Biomolecular Resource Facilities Metagenomics Research Group (ABRF MGRG) that focuses on whole genome shotgun sequencing of extreme and uniqu...
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ftcdlib:qt1kc8j7m2 2023-05-15T13:43:56+02:00 Genomic methods and microbiological technologies for profiling novel and extreme environments for the extreme microbiome project (XMP) Tighe, S Afshinnekoo, E Rock, TM McGrath, K Alexander, N McIntyre, A Ahsanuddins, S Bezdan, D Green, SJ Joye, S Johnson, SS Baldwin, DA Bivens, N Ajami, N Carmical, JR Herriott, IC Colwell, R Donia, M Foox, J Greenfield, N Hunter, T Hoffman, J Hyman, J Jorgensen, E Krawczyk, D Lee, J Levy, S Garcia-Reyero, N Settles, M Thomas, K Gómez, F Schriml, L Kyrpides, N Zaikova, E Penterman, J Mason, CE 31 - 39 2017-04-01 application/pdf http://www.escholarship.org/uc/item/1kc8j7m2 english eng eScholarship, University of California qt1kc8j7m2 http://www.escholarship.org/uc/item/1kc8j7m2 public Tighe, S; Afshinnekoo, E; Rock, TM; McGrath, K; Alexander, N; McIntyre, A; et al.(2017). Genomic methods and microbiological technologies for profiling novel and extreme environments for the extreme microbiome project (XMP). Journal of Biomolecular Techniques, 28(1), 31 - 39. doi:10.7171/jbt.17-2801-004. Lawrence Berkeley National Laboratory: Retrieved from: http://www.escholarship.org/uc/item/1kc8j7m2 article 2017 ftcdlib https://doi.org/10.7171/jbt.17-2801-004 2018-07-13T22:57:40Z © 2017, Association of Biomolecular Resource Facilities. All rights reserved. The Extreme Microbiome Project (XMP) is a project launched by the Association of Biomolecular Resource Facilities Metagenomics Research Group (ABRF MGRG) that focuses on whole genome shotgun sequencing of extreme and unique environments using a wide variety of biomolecular techniques. The goals are multifaceted, including development and refinement of new techniques for the following: 1) the detection and characterization of novel microbes, 2) the evaluation of nucleic acid techniques for extremophilic samples, and 3) the identification and implementation of the appropriate bioinformatics pipelines. Here, we highlight the different ongoing projects that we have been working on, as well as details on the various methods we use to characterize the microbiome and metagenome of these complex samples. In particular, we present data of a novel multienzyme extraction protocol that we developed, called Polyzyme or MetaPolyZyme. Presently, the XMP is characterizing sample sites around the world with the intent of discovering new species, genes, and gene clusters. Once a project site is complete, the resulting data will be publically available. Sites include Lake Hillier in Western Australia, the “Door to Hell” crater in Turkmenistan, deep ocean brine lakes of the Gulf of Mexico, deep ocean sediments from Greenland, permafrost tunnels in Alaska, ancient microbial biofilms from Antarctica, Blue Lagoon Iceland, Ethiopian toxic hot springs, and the acidic hypersaline ponds in Western Australia. Article in Journal/Newspaper Antarc* Antarctica Greenland Iceland permafrost Alaska University of California: eScholarship Blue Lagoon ENVELOPE(-22.449,-22.449,63.880,63.880) Greenland Journal of Biomolecular Techniques : JBT 28 1 31 39 |
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Open Polar |
collection |
University of California: eScholarship |
op_collection_id |
ftcdlib |
language |
English |
description |
© 2017, Association of Biomolecular Resource Facilities. All rights reserved. The Extreme Microbiome Project (XMP) is a project launched by the Association of Biomolecular Resource Facilities Metagenomics Research Group (ABRF MGRG) that focuses on whole genome shotgun sequencing of extreme and unique environments using a wide variety of biomolecular techniques. The goals are multifaceted, including development and refinement of new techniques for the following: 1) the detection and characterization of novel microbes, 2) the evaluation of nucleic acid techniques for extremophilic samples, and 3) the identification and implementation of the appropriate bioinformatics pipelines. Here, we highlight the different ongoing projects that we have been working on, as well as details on the various methods we use to characterize the microbiome and metagenome of these complex samples. In particular, we present data of a novel multienzyme extraction protocol that we developed, called Polyzyme or MetaPolyZyme. Presently, the XMP is characterizing sample sites around the world with the intent of discovering new species, genes, and gene clusters. Once a project site is complete, the resulting data will be publically available. Sites include Lake Hillier in Western Australia, the “Door to Hell” crater in Turkmenistan, deep ocean brine lakes of the Gulf of Mexico, deep ocean sediments from Greenland, permafrost tunnels in Alaska, ancient microbial biofilms from Antarctica, Blue Lagoon Iceland, Ethiopian toxic hot springs, and the acidic hypersaline ponds in Western Australia. |
format |
Article in Journal/Newspaper |
author |
Tighe, S Afshinnekoo, E Rock, TM McGrath, K Alexander, N McIntyre, A Ahsanuddins, S Bezdan, D Green, SJ Joye, S Johnson, SS Baldwin, DA Bivens, N Ajami, N Carmical, JR Herriott, IC Colwell, R Donia, M Foox, J Greenfield, N Hunter, T Hoffman, J Hyman, J Jorgensen, E Krawczyk, D Lee, J Levy, S Garcia-Reyero, N Settles, M Thomas, K Gómez, F Schriml, L Kyrpides, N Zaikova, E Penterman, J Mason, CE |
spellingShingle |
Tighe, S Afshinnekoo, E Rock, TM McGrath, K Alexander, N McIntyre, A Ahsanuddins, S Bezdan, D Green, SJ Joye, S Johnson, SS Baldwin, DA Bivens, N Ajami, N Carmical, JR Herriott, IC Colwell, R Donia, M Foox, J Greenfield, N Hunter, T Hoffman, J Hyman, J Jorgensen, E Krawczyk, D Lee, J Levy, S Garcia-Reyero, N Settles, M Thomas, K Gómez, F Schriml, L Kyrpides, N Zaikova, E Penterman, J Mason, CE Genomic methods and microbiological technologies for profiling novel and extreme environments for the extreme microbiome project (XMP) |
author_facet |
Tighe, S Afshinnekoo, E Rock, TM McGrath, K Alexander, N McIntyre, A Ahsanuddins, S Bezdan, D Green, SJ Joye, S Johnson, SS Baldwin, DA Bivens, N Ajami, N Carmical, JR Herriott, IC Colwell, R Donia, M Foox, J Greenfield, N Hunter, T Hoffman, J Hyman, J Jorgensen, E Krawczyk, D Lee, J Levy, S Garcia-Reyero, N Settles, M Thomas, K Gómez, F Schriml, L Kyrpides, N Zaikova, E Penterman, J Mason, CE |
author_sort |
Tighe, S |
title |
Genomic methods and microbiological technologies for profiling novel and extreme environments for the extreme microbiome project (XMP) |
title_short |
Genomic methods and microbiological technologies for profiling novel and extreme environments for the extreme microbiome project (XMP) |
title_full |
Genomic methods and microbiological technologies for profiling novel and extreme environments for the extreme microbiome project (XMP) |
title_fullStr |
Genomic methods and microbiological technologies for profiling novel and extreme environments for the extreme microbiome project (XMP) |
title_full_unstemmed |
Genomic methods and microbiological technologies for profiling novel and extreme environments for the extreme microbiome project (XMP) |
title_sort |
genomic methods and microbiological technologies for profiling novel and extreme environments for the extreme microbiome project (xmp) |
publisher |
eScholarship, University of California |
publishDate |
2017 |
url |
http://www.escholarship.org/uc/item/1kc8j7m2 |
op_coverage |
31 - 39 |
long_lat |
ENVELOPE(-22.449,-22.449,63.880,63.880) |
geographic |
Blue Lagoon Greenland |
geographic_facet |
Blue Lagoon Greenland |
genre |
Antarc* Antarctica Greenland Iceland permafrost Alaska |
genre_facet |
Antarc* Antarctica Greenland Iceland permafrost Alaska |
op_source |
Tighe, S; Afshinnekoo, E; Rock, TM; McGrath, K; Alexander, N; McIntyre, A; et al.(2017). Genomic methods and microbiological technologies for profiling novel and extreme environments for the extreme microbiome project (XMP). Journal of Biomolecular Techniques, 28(1), 31 - 39. doi:10.7171/jbt.17-2801-004. Lawrence Berkeley National Laboratory: Retrieved from: http://www.escholarship.org/uc/item/1kc8j7m2 |
op_relation |
qt1kc8j7m2 http://www.escholarship.org/uc/item/1kc8j7m2 |
op_rights |
public |
op_doi |
https://doi.org/10.7171/jbt.17-2801-004 |
container_title |
Journal of Biomolecular Techniques : JBT |
container_volume |
28 |
container_issue |
1 |
container_start_page |
31 |
op_container_end_page |
39 |
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1766195187488391168 |